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高温胁迫下厚壳贻贝(Mytilus coruscus)消化腺代谢组学研究 被引量:1
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作者 郭锋 王玉霞 +5 位作者 董震宇 姚荣辉 王伟峰 叶莹莹 严小军 郭宝英 《海洋与湖沼》 CAS CSCD 北大核心 2024年第1期171-181,共11页
在全球变暖的背景下,海水温度不断升高、海洋暖化逐渐加剧,高温严重影响着海洋生物的各种生命过程,但对于海洋双壳贝类如何应对热应激的研究仍然不足。为此,开展了以18℃(CT)为对照组在26℃(ST)和33℃(HT)下对厚壳贻贝消化腺组织进行了... 在全球变暖的背景下,海水温度不断升高、海洋暖化逐渐加剧,高温严重影响着海洋生物的各种生命过程,但对于海洋双壳贝类如何应对热应激的研究仍然不足。为此,开展了以18℃(CT)为对照组在26℃(ST)和33℃(HT)下对厚壳贻贝消化腺组织进行了急性热胁迫下的代谢组学分析,以便于研究其代谢反应。采用LC-MS/MS技术,并结合生物信息分析手段对差异代谢物进行筛选,并分析确定相关的代谢通路的变化,共有2532种代谢物在厚壳贻贝消化腺中被鉴定。KEGG富集分析用于探索差异代谢物的潜在代谢途径,共有29条代谢通路被显著富集,与对照组相比,ST组显著富集于牛磺酸和次牛磺酸代谢、神经活性配体-受体相互作用、鞘脂类代谢和视黄醇代谢等代谢通路;HT组显著富集于酪氨酸代谢、亚油酸代谢、丙氨酸新陈代谢、酪氨酸代谢、色氨酸代谢、苯丙氨酸代谢等代谢通路。研究结果显示,厚壳贻贝消化腺主要通过调节色氨酸代谢、酪氨酸代谢、鞘脂代谢、苯丙氨酸代谢、氧化磷酸化,脂肪酸、赖氨酸降解等信号通路应对热应激,从而帮助维持身体内部环境的稳定状态。上述研究为多视角探究厚壳贻贝耐热机制与应对环境中温度变化的适应性进化提供理论基础。 展开更多
关键词 厚壳贻贝(mytilus coruscus) 消化腺 高温胁迫 代谢组学
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Genetic Analysis of Cultured and Wild Populations of Mytilus coruscus Based on Mitochondrial DNA 被引量:18
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作者 沈玉帮 李家乐 冯冰冰 《Zoological Research》 CAS CSCD 北大核心 2009年第3期240-246,共7页
DNA sequences from the mitochondrial gene cytochrome oxidase subunit I (mtDNA CO I) were used to estimate the genetic variability in two wild populations and two cultured populations of the hard shelled mussel, Myti... DNA sequences from the mitochondrial gene cytochrome oxidase subunit I (mtDNA CO I) were used to estimate the genetic variability in two wild populations and two cultured populations of the hard shelled mussel, Mytilus coruscus. Thirty haplotypes were identified in the four populations. The cultured populations exhibited a lower number of haplotypes and genetic diversity than those of the wild populations, suggesting that a small number of effective founding breeders contributed to the genetic variation of the cultured populations. No significant differentiation was observed between the cultured population and local wild population, implying that persistent gene flow occurred in these populations. This genetic survey is intended as a baseline for future genetic monitoring of M. coruseus aquaculture stocks. 展开更多
关键词 mytilus coruscus Population differentiation Genetic diversity mtDNA COI gene
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A Preliminary Analysis on the Construction and Expression of Eukaryotic Expression Vectors of Mytilin and Myticin from Mytilus coruscus 被引量:10
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作者 武梅 范美华 +2 位作者 廖智 石戈 王日昕 《Agricultural Science & Technology》 CAS 2010年第7期72-75,共4页
[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides (mytilin and myticin) from Mytilus coruscus.[Method] By the screening of antibacterial pept... [Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides (mytilin and myticin) from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this. 展开更多
关键词 mytilus coruscus Antibiotic peptide Mytilin Myticin Expression vector
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Biochemical response of the mussel Mytilus coruscus(Mytiloida:Mytilidae) exposed to in vivo sub-lethal copper concentrations 被引量:9
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作者 李一峰 顾忠旗 +2 位作者 刘红 沈和定 杨金龙 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第5期738-745,共8页
Many aquatic organisms are negatively affected by exposure to high copper concentrations. We investigated the biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) to copper exposure. In vivo b... Many aquatic organisms are negatively affected by exposure to high copper concentrations. We investigated the biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) to copper exposure. In vivo bioassays using M. coruscus and different copper concentrations were conducted. The activity of six biomarkers, namely superoxide dismutase (SOD), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were measured. Survival rates decreased with increased copper concentrations and exposure times. The LCs0 values at 48, 72, and 96 h exposure were 0.48, 0.37, and 0.32 rag/L, respectively. Within digestive glands, CAT activity increased with increasing Cu concentrations. The activity of AKP showed no significant change, while the remaining four enzymes showed decreasing activity with increasing Cu concentrations. Within the gills, AKP activity increased when the Cu concentration was 0.05 mg/L, but showed no significant changes at higher concentrations. Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration. The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L. GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L. Within the adductor muscle, AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations. ACP activity within adductor muscle tissue showed no change, while activities of CAT, GOT and GPT decreased with increasing Cu concentrations. SOD activity within the adductor muscle tissue significantly decreased at the 0.02, 0.05 and 0.2 mg/L exposure concentrations. Our results show tissue specific differences for the six biomarkers in for M. coruscus. Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated, and are essential preliminary steps in development of in vivo bioassays. 展开更多
关键词 mytilus coruscus COPPER biomarker antioxidant enzymes metabolic enzyme
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海水酸化对厚壳贻贝(Mytilus coruscus)外套膜代谢组的急性影响 被引量:2
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作者 范孝俊 王莹 +6 位作者 蔡吕萍 黄键锋 刘菲 李迎澳 张晓林 严小军 廖智 《海洋与湖沼》 CAS CSCD 北大核心 2023年第3期921-933,共13页
海洋酸化是当前全球面临的最为紧迫的环境问题之一,已显现出对海洋生物的严重影响。贻贝是我国重要的经济贝类之一,但目前尚缺乏海洋酸化背景下厚壳贻贝的分子响应相关研究。为此,对厚壳贻贝外套膜组织开展了急性酸化条件下的代谢组学分... 海洋酸化是当前全球面临的最为紧迫的环境问题之一,已显现出对海洋生物的严重影响。贻贝是我国重要的经济贝类之一,但目前尚缺乏海洋酸化背景下厚壳贻贝的分子响应相关研究。为此,对厚壳贻贝外套膜组织开展了急性酸化条件下的代谢组学分析,以期了解厚壳贻贝在酸化条件下,其外套膜组织的代谢物组成和含量变化。采用超高压液相色谱-串联质谱技术,结合代谢物数据库搜索和鉴定,从厚壳贻贝外套膜中鉴定代谢物总计7882种。与对照组相比,酸化处理后的厚壳贻贝外套膜组织中共有497种代谢物含量发生显著变化(P<0.05),其中,320种差异代谢物显著上调,177种显著下调。差异代谢物富集的代谢通路主要包括脂代谢、信号转导、维生素代谢、核酸代谢、氨基酸代谢以及ABC转运体。结合外套膜游离氨基酸组成分析以及抗氧化活性分析,推测厚壳贻贝通过激活尿素合成、增强细胞膜流动性以及加强渗透压调节和钙离子转运等方式来应对海洋酸化的威胁。上述研究为深入了解贻贝应对海洋酸化的分子策略,以及在海洋酸化大背景下的贻贝健康养殖奠定了基础。 展开更多
关键词 厚壳贻贝(mytilus coruscus) 外套膜 海洋酸化 代谢组学
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Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection 被引量:1
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作者 Chen PAN Zhi LIAO +7 位作者 Jianyu HE Zhongqi GU Chunyue WANG Meihua FAN Isabella BUTTINO Baoying GUO Xiaojun YAN Xiaolin ZHANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第3期1121-1134,共14页
Carnosine and its analogues are histidine-containing dipeptides(HCDs)playing diverse functions in vertebrates.However,the distribution and the metabolism of carnosine in invertebrates are still unknown.In this study,M... Carnosine and its analogues are histidine-containing dipeptides(HCDs)playing diverse functions in vertebrates.However,the distribution and the metabolism of carnosine in invertebrates are still unknown.In this study,Mytilus coruscus,a shellfish with important economic value in China,was selected for the investigation of HCD content and the expression profiling of carnosine-related genes in various mussel tissues.Quantification of HCD by amino acids analyzer revealed a low concentration of anserine in muscular tissues in Mytilus,indicating the presence of HCD even in an invertebrate.mRNA expression of five carnosine metabolic-related genes was profiled in various tissues,and the results highlighted the relative higher expression level of these genes in muscular tissues.Considering the fact that beta-alanine supplementation can increase the HCD content in vertebrates,a beta-alanine injection was performed and the changes of HCD concentration and the mRNA expression of carnosine related genes were investigated in five mussel tissues.The results revealed the increase of HCD concentration,as well as the up-regulated expression level of related genes,in tested tissues of beta-alanine injected mussels.Transcriptomic analysis for the whole soft tissue of mussel before and after beta-alanine injection were performed,and 3569 differential expression genes(DEGs)were identified in the beta-alanine injected group when compared to their expression levels in the control.These data indicated the complex eff ects of betaalanine on M.coruscus metabolism,and those DEGs enriched in pathways of cancers,muscle contraction,and tyrosine metabolism highlighted the possible functions of beta-alanine in cell proliferation,sports,and melanogenesis,respectively. 展开更多
关键词 mytilus coruscus BETA-ALANINE CARNOSINE histidine-containing dipeptides TRANSCRIPTOME
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基于静态模型的五种苯系物在海水和厚壳贻贝中的消除规律
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作者 宋立玲 周旭 +3 位作者 廖思扬 於佩佩 胡芬静 周秀锦 《湖北农业科学》 2023年第6期29-32,共4页
对静态模型中的苯(BZ)、甲苯(TU)、对二甲苯(PX)、邻二甲苯(MX)和间二甲苯(OX)5种苯系物在海水和厚壳贻贝(Mytilus coruscus)中的消除规律进行研究,在(20±0.5)℃环境温度下,以海水和厚壳贻贝为研究对象,分别添加一定浓度的5种苯系... 对静态模型中的苯(BZ)、甲苯(TU)、对二甲苯(PX)、邻二甲苯(MX)和间二甲苯(OX)5种苯系物在海水和厚壳贻贝(Mytilus coruscus)中的消除规律进行研究,在(20±0.5)℃环境温度下,以海水和厚壳贻贝为研究对象,分别添加一定浓度的5种苯系物,用顶空进样-气相色谱氢火焰离子化检测器法(HS-GCFID)分析不同时间点的5种苯系物在海水和厚壳贻贝中的残留浓度。结果表明,海水和厚壳贻贝中苯系物各组分浓度随时间变化规律基本相同,海水中5种苯系物的消除速率由大到小依次为MX、PX、TU、BZ、OX,厚壳贻贝中5种苯系物的消除速率由大到小依次为TU、BZ、MX、OX、PX,厚壳贻贝与海水的消除速率不同,其原因可能是厚壳贻贝富含脂肪、蛋白质等复杂基质,苯系物与厚壳贻贝基质的结合程度较强。 展开更多
关键词 HS-GC-FID 苯系物 海水 厚壳贻贝(mytilus coruscus)
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High Fischer ratio oligopeptides from hard-shelled mussel:Preparation and hepatoprotective effect against acetaminophen-induced liver injury in mice
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作者 Shuo-Lei Zheng Yue-Zheng Wang +3 位作者 Yu-Qin Zhao Chang-Feng Chi Wang-Yu Zhu Bin Wang 《Food Bioscience》 SCIE 2023年第3期1551-1564,共14页
High Fischer ratio oligopeptides(HFOPs)have a variety of physiological activities,but there are few literatures reporting the hepatoprotective effects of HFOPs against acetaminophen(APAP)-induced liver injury.The ob-j... High Fischer ratio oligopeptides(HFOPs)have a variety of physiological activities,but there are few literatures reporting the hepatoprotective effects of HFOPs against acetaminophen(APAP)-induced liver injury.The ob-jectives of this study were to establish the preparation process of HFOPs from hard-shelled mussel(Mytilus coruscus)(HFOPs-HM)and investigate the hepatoprotective effects and potential mechanisms of HFOPs-HM against APAP-induced liver injury in mice.Therefore,trypsin and papain were selected as the endo and exog-enous proteases for preparing HFOPs-HM according to the degree of hydrolysis(DH)of hard-shelled mussel hydrolysates,and activated carbon of XHJ-200 displayed the best effect to remove aromatic amino acids ac-cording to the Fischer ratio(OD220/OD280)and its parameters were optimized as adsorption time of 3.0 h and solid-liquid ratio of 1:10.The HFOPs-HM’s Fischer ratio and average molecular weight were 26.03(>20)and 878.54 Da,respectively.Furthermore,a mice model of liver injury induced by APAP was established.HFOPs-HM could significantly improve liver index and histopathological features,decrease the serum alanine aminotrans-ferase(ALT)and aspartate transaminase(AST)activities,increase the glutathione content and superoxide dis-mutase(SOD)and catalase(CAT)activities.Further research demonstrated that HFOPs-HM could alleviate liver oxidative stress in liver tissue via activating the Nrf2 pathway to initiate the intracellular antioxidant enzyme system.In addition,HFOPs-HM also suppressed liver inflammation by downregulating the secretion of TNF-α,IL-1β,and IL-6.Thus,HFOPs-HM could serve as an auxiliary functional dietary molecule applied in function products to alleviate drug-induced liver injury. 展开更多
关键词 Hard-shelled mussel(mytilus coruscus) High Fischer ratio oligopeptides(HFOPs) Hepatoprotective effect Acetaminophen(APAP)-Induced liver injury Oxidative stress Inflammatory
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