The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demon...The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.展开更多
Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-P...Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.展开更多
AIM:The heptadecapeptide nociceptin alias orphanin FQ is the endogenous agonist of opioid receptor-likel receptor. It is involved in modulation of pain and cognition.High blood level was reported in patients with acut...AIM:The heptadecapeptide nociceptin alias orphanin FQ is the endogenous agonist of opioid receptor-likel receptor. It is involved in modulation of pain and cognition.High blood level was reported in patients with acute and chronic pain, and in Wilson disease.An accidental observation led us to investigate nociceptin in hepatocellular carcinoma. METHODS:Plasma nociceptin level was measured by radioimmunoassay,aprotinin was used as protease inhibitor. Hepatocellular carcinoma was diagnosed by laboratory, ultrasound,other imaging,and confirmed by fine needle biopsy.Results were compared to healthy controls and patients with other chronic liver diseases. RESULTS:Although nociceptin levels were elevated in patients with Wilson disease (14.0±2.7 pg/mL,n=26), primary biliary cirrhosis (12.1±3.2 pg/mL,n=21) and liver cirrhosis (12.8±4.0 pg/mL,n=15) compared to the healthy controls (9.2±1.8 pg/mL,n=29, P<0.001 for each),in patients with hepatocellular carcinoma a ten-fold increase was found (105.9±14.4 pg/mL,n=29,P<0.0001).High plasma levels were found in each hepatocellular carcinoma patient including those with normal alpha fetoprotein and those with pain (104.9±14.9 pg/mL,n=12) and without (107.7±14.5 pg/mL,n=6). CONCLUSION:A very high nociceptin plasma level seems to be an indicator for hepatocellular carcinoma.Further research is needed to clarify the mechanism and clinical significance of this novel finding.展开更多
AIM:Although liver cirrhosis is a predisposing factor for hepatocellular carcinoma (HCC),relatively few reports are available on HCC in primary biliary cirrhosis.High plasma nociceptin (N/OFQ) level has been shown in ...AIM:Although liver cirrhosis is a predisposing factor for hepatocellular carcinoma (HCC),relatively few reports are available on HCC in primary biliary cirrhosis.High plasma nociceptin (N/OFQ) level has been shown in Wilson disease and in patients with acute and chronic pain. METHODS:We report a follow-up case of HCC,which developed in a patient with primary biliary cirrhosis.The tumor appeared 18 years after the diagnosis of PBC and led to death within two years.Alfa fetoprotein and serum nociceptin levels were monitored before and during the development of HCC. Nociceptin content was also measured in the tumor tissue. RESULTS:The importance and the curiosity of the presented case was the novel finding of the progressive elevation of plasma nociceptin level up to 17-fold (172 pg/mL) above the baseline (9.2±1.8 pg/mL),parallel with the elevation of alpha fetoprotein (from 13 ng/mL up to 3 480 ng/mL) during tumor development.Nociceptin content was more than 15-fold higher in the neoplastic tissue (0.16 pg/mg) than that in the tumor- free liver tissue samples (0.01 pg/mg) taken during the autopsy. CONCLUSION:Results are in concordance with our previous observation that a very high plasma nociceptin level may be considered as an indicator for hepatocellular carcinoma.展开更多
文摘The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.
文摘Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.
文摘AIM:The heptadecapeptide nociceptin alias orphanin FQ is the endogenous agonist of opioid receptor-likel receptor. It is involved in modulation of pain and cognition.High blood level was reported in patients with acute and chronic pain, and in Wilson disease.An accidental observation led us to investigate nociceptin in hepatocellular carcinoma. METHODS:Plasma nociceptin level was measured by radioimmunoassay,aprotinin was used as protease inhibitor. Hepatocellular carcinoma was diagnosed by laboratory, ultrasound,other imaging,and confirmed by fine needle biopsy.Results were compared to healthy controls and patients with other chronic liver diseases. RESULTS:Although nociceptin levels were elevated in patients with Wilson disease (14.0±2.7 pg/mL,n=26), primary biliary cirrhosis (12.1±3.2 pg/mL,n=21) and liver cirrhosis (12.8±4.0 pg/mL,n=15) compared to the healthy controls (9.2±1.8 pg/mL,n=29, P<0.001 for each),in patients with hepatocellular carcinoma a ten-fold increase was found (105.9±14.4 pg/mL,n=29,P<0.0001).High plasma levels were found in each hepatocellular carcinoma patient including those with normal alpha fetoprotein and those with pain (104.9±14.9 pg/mL,n=12) and without (107.7±14.5 pg/mL,n=6). CONCLUSION:A very high nociceptin plasma level seems to be an indicator for hepatocellular carcinoma.Further research is needed to clarify the mechanism and clinical significance of this novel finding.
文摘AIM:Although liver cirrhosis is a predisposing factor for hepatocellular carcinoma (HCC),relatively few reports are available on HCC in primary biliary cirrhosis.High plasma nociceptin (N/OFQ) level has been shown in Wilson disease and in patients with acute and chronic pain. METHODS:We report a follow-up case of HCC,which developed in a patient with primary biliary cirrhosis.The tumor appeared 18 years after the diagnosis of PBC and led to death within two years.Alfa fetoprotein and serum nociceptin levels were monitored before and during the development of HCC. Nociceptin content was also measured in the tumor tissue. RESULTS:The importance and the curiosity of the presented case was the novel finding of the progressive elevation of plasma nociceptin level up to 17-fold (172 pg/mL) above the baseline (9.2±1.8 pg/mL),parallel with the elevation of alpha fetoprotein (from 13 ng/mL up to 3 480 ng/mL) during tumor development.Nociceptin content was more than 15-fold higher in the neoplastic tissue (0.16 pg/mg) than that in the tumor- free liver tissue samples (0.01 pg/mg) taken during the autopsy. CONCLUSION:Results are in concordance with our previous observation that a very high plasma nociceptin level may be considered as an indicator for hepatocellular carcinoma.