The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2）...The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2） solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine （BA） and α-naphthaleneacetic acid （NAA） added to Murashige and Skoog （MS） medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips （96.7%, 9.8） and leaves （90.0%, 8.7） were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid （IBA） with the highest rooting frequency （93.3%） and numbers of roots/explant （8.2）. For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat （1： 1）. After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana ＇Neva＇.展开更多
Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one ...Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one of the main obstacles to continuous cropping of Populus×euramericana ‘Neva’,we examined gene expression dynamics in Neva leaves induced by pHBA.The expression of genes related to photosynthesis and respiration changed,while mRNA involved in regulating gene expression during allelopathy was degraded.Some miRNAs that are involved in plant allelopathy target crucial genes for regulating reactive oxygen species.Moreover,coexpression regulatory networks were constructed based on profiles of the identified miRNA-target interactions and the differentially expressed miRNA-target pairs.These findings provide a mechanistic framework for understanding allelopathy in plants.展开更多
Extreme waves have a profound impact on coastal infrastructure;thus,understanding the variation law of risky analysis and disaster prevention in coastal zones is necessary.This paper analyzed the spatiotemporal charac...Extreme waves have a profound impact on coastal infrastructure;thus,understanding the variation law of risky analysis and disaster prevention in coastal zones is necessary.This paper analyzed the spatiotemporal characteristics of extreme wave heights adjacent to China from 1979 to 2018 based on the ERA5 datasets.Nonstationary extreme value analysis is undertaken in eight repre-sentative points to investigate the trends in the values of 50-and 100-year wave heights.Results show that the mean value of extreme waves is the largest in the eastern part of Taiwan Island and the smallest in the Bohai Sea from 1979 to 2018.Only the extreme wave height in the northeastern part of Taiwan Island shows a significant increase trend in the study area.Nonstationary analysis shows remarkable variations in the values of 50-and 100-year significant wave heights in eight points.Considering the annual mean change,E1,E2,S1,and S2 present an increasing trend,while S3 shows a decreasing trend.Most points for the seasonal mean change demon-strate an increasing trend in spring and winter,while other points show a decreasing trend in summer and autumn.Notably,the E1 point growth rate is large in autumn,which is related to the change in typhoon intensity and the northward movement of the typhoon path.展开更多
The absorption and enrichment of heavy metal elements by leaves of Populus×euramericana cv.’Neva’ were analyzed through content assessing of heavy metal elements in soils of three forest lands at Fangshan Distr...The absorption and enrichment of heavy metal elements by leaves of Populus×euramericana cv.’Neva’ were analyzed through content assessing of heavy metal elements in soils of three forest lands at Fangshan District in the southern suburb of Beijing. These forest lands with P.×euramericana cv. ’Neva’plantation were around with the various sources of pollution such as cement mill,stone mill, sewage and garbage.Some of heavy metal elements such as Cd,Pb,Cu,and Zn were found in the soil samples and leaves of P.×euramericana cv.’Neva’ grown on the three forest lands which were investigated.The results of investigation showed that the soils of three forest lands were polluted with Pb, Cd,Cu and Zn in varying degrees.The contents of Pb,Cu and Zn in soils were significantly different among the three forest lands,whereas the average content of Cd(0.077 6 mg·kg<sup>-1</sup>) was less but that of Pb,Cu,and Zn(37.61,26.25 and 90.3 mg·kg<sup>-1</sup> ) were more than the average background value of Beijing.The contents of Pb,Cd,Cu and Zn in leaves of P.×euramericana cv.’Neva’ were different among three forestlands.With the changes of heavy metal contents in soil samples,there was no regularity on the changes of different heavy metal contents in leaves of P.×euramericana cv.’Neva’ grown on different forest land.P.×euramericana cv.’Neva’ can absorb and accumulate Pb,Cd,Cu and Zn in soil but showed different capacities of absorption and accumulation with the order of Cd】Zn】Cu】Pb.Especially,its absorption and accumulation capacity for Cd was strong with maximum enrichment factor higher than 16 and showed high accumulation on low background,whereas its enrichment capacities for Pb and Cu were relatively poorer with average enrichment factor of less than 1.At different forest lands,there existed great difference on absorption capacity of P.×euramericana cv.’Neva’ with the same heavy metal.展开更多
文摘The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2） solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine （BA） and α-naphthaleneacetic acid （NAA） added to Murashige and Skoog （MS） medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips （96.7%, 9.8） and leaves （90.0%, 8.7） were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid （IBA） with the highest rooting frequency （93.3%） and numbers of roots/explant （8.2）. For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat （1： 1）. After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana ＇Neva＇.
基金the National Public Welfare Industry Research Project of China(201504406)National Natural Science Foundation of China(31770706)+1 种基金National Natural Science Foundation of China(31870379)Science Research Projects of Facility Horticulture Laboratory of Universities in Shandong(2018YY031)。
文摘Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one of the main obstacles to continuous cropping of Populus×euramericana ‘Neva’,we examined gene expression dynamics in Neva leaves induced by pHBA.The expression of genes related to photosynthesis and respiration changed,while mRNA involved in regulating gene expression during allelopathy was degraded.Some miRNAs that are involved in plant allelopathy target crucial genes for regulating reactive oxygen species.Moreover,coexpression regulatory networks were constructed based on profiles of the identified miRNA-target interactions and the differentially expressed miRNA-target pairs.These findings provide a mechanistic framework for understanding allelopathy in plants.
基金support of the Natural Science Foundation of China(No.51909114)the Major Research Grant(Nos.U1806227,U1906231)from the National Natural Science Foundation of China(NSFC).
文摘Extreme waves have a profound impact on coastal infrastructure;thus,understanding the variation law of risky analysis and disaster prevention in coastal zones is necessary.This paper analyzed the spatiotemporal characteristics of extreme wave heights adjacent to China from 1979 to 2018 based on the ERA5 datasets.Nonstationary extreme value analysis is undertaken in eight repre-sentative points to investigate the trends in the values of 50-and 100-year wave heights.Results show that the mean value of extreme waves is the largest in the eastern part of Taiwan Island and the smallest in the Bohai Sea from 1979 to 2018.Only the extreme wave height in the northeastern part of Taiwan Island shows a significant increase trend in the study area.Nonstationary analysis shows remarkable variations in the values of 50-and 100-year significant wave heights in eight points.Considering the annual mean change,E1,E2,S1,and S2 present an increasing trend,while S3 shows a decreasing trend.Most points for the seasonal mean change demon-strate an increasing trend in spring and winter,while other points show a decreasing trend in summer and autumn.Notably,the E1 point growth rate is large in autumn,which is related to the change in typhoon intensity and the northward movement of the typhoon path.
文摘The absorption and enrichment of heavy metal elements by leaves of Populus×euramericana cv.’Neva’ were analyzed through content assessing of heavy metal elements in soils of three forest lands at Fangshan District in the southern suburb of Beijing. These forest lands with P.×euramericana cv. ’Neva’plantation were around with the various sources of pollution such as cement mill,stone mill, sewage and garbage.Some of heavy metal elements such as Cd,Pb,Cu,and Zn were found in the soil samples and leaves of P.×euramericana cv.’Neva’ grown on the three forest lands which were investigated.The results of investigation showed that the soils of three forest lands were polluted with Pb, Cd,Cu and Zn in varying degrees.The contents of Pb,Cu and Zn in soils were significantly different among the three forest lands,whereas the average content of Cd(0.077 6 mg·kg<sup>-1</sup>) was less but that of Pb,Cu,and Zn(37.61,26.25 and 90.3 mg·kg<sup>-1</sup> ) were more than the average background value of Beijing.The contents of Pb,Cd,Cu and Zn in leaves of P.×euramericana cv.’Neva’ were different among three forestlands.With the changes of heavy metal contents in soil samples,there was no regularity on the changes of different heavy metal contents in leaves of P.×euramericana cv.’Neva’ grown on different forest land.P.×euramericana cv.’Neva’ can absorb and accumulate Pb,Cd,Cu and Zn in soil but showed different capacities of absorption and accumulation with the order of Cd】Zn】Cu】Pb.Especially,its absorption and accumulation capacity for Cd was strong with maximum enrichment factor higher than 16 and showed high accumulation on low background,whereas its enrichment capacities for Pb and Cu were relatively poorer with average enrichment factor of less than 1.At different forest lands,there existed great difference on absorption capacity of P.×euramericana cv.’Neva’ with the same heavy metal.
