The Comparative Performance of Nutrient-Film Technique and Deep-Water Culture Method of Hydroponics for GREENBOX Technology

With the rising pressures on food security, GREENBOX technology was developed as an avenue for fresh leafy vegetable crop production in urban settings. GREENBOX units were designed to be thermally insulated and climate controlled, with an artificial lighting source that utilized soilless cultivation techniques. Previous studies conducted on GREENBOX technology used the Nutrient Film Technique (NFT);however, various hydroponic methods exist, such as the Deep-Water Culture (DWC) method being the most used. The APS Laboratory for Sustainable Food at Florida Gulf Coast University (FGCU) compared the crop growth performance between DWC and NFT systems using GREENBOX technology. The following study monitored environmental conditions and compared productivity and biomass data of Rex Butterhead Lettuce crops between DWC and NFT systems. We assembled two GREENBOX units using commercially available materials and the standard nutrient solution for fertigation. The crops grown in DWC and NFT were in a 4 × 6 configuration. The DWC and NFT systems were used to grow Lettuce Lactuca sativa “Rex Butterhead” over 30 days to full bloom from prepared plugs grown for 14 days. We collected environmental data including Photosynthetic Photon Flux Density (PPFD, μmol/m2∙s), Daily Light Integral (DLI, mol/ m2∙d), temperature (˚C), relative humidity (%), and Vapor Pressure Deficit (VPD, kPa). We collected lettuce crop growth data, which included wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (μmol/m2). We derived data, including the Specific Leaf Area (SLA, cm2/g) and biomass productivity (kg/m2), from previously collected data. We used descriptive statistics to present the collected data. A paired t-test was performed to understand the differences in biomass and productivity parameters between the DWC and NFT-grown lettuce crops. Both the DWC and NFT-grown crops could grow lettuce crops to harvest weight at full bloom. Observed data demonstrated that the biomass parameters and productivity did not differ significantly between the two hydroponics techniques. Therefore, we believe both hydroponic methods may be similar in growth performance and may be used in future iterations of GREENBOX design and prove suitable for fresh vegetable crop production in urban settings.

The Comparative Performance of Soil-Based Systems with Hydroponics

Conventional soil-based agriculture is resource-intensive, utilizing large amounts of land and water, thereby placing a strain on Earth’s natural resources. Soil-based agricultural techniques create environmental issues such as soil degradation, deforestation, and groundwater pollution from the mass implementation of fertilizers and pesticides. Agricultural crop production using hydroponics has shown promise to be less resource intensive and provide a faster turnaround in crop production. Soilless cultivation using hydroponics promises to relieve some pressure on Earth’s ecosystems and resources by utilizing lesser land and water footprint. The APS Laboratory for Sustainable Food at Florida Gulf Coast University (FGCU) compared the growth of Lettuce Lactuca sativa “Rex Butterhead” crop grown using soil and soilless methods to analyze the growth performance in each setting. Crops grown in the soil-based medium were raised in the FGCU Food Forest, used a mix of soil and potting mix, watered regularly, and followed standard Integrated Pest Management (IPM) practices. Crops grown hydroponically were grown in a thermally insulated grow tent with an artificial lighting source, ventilation, environmental controls, and the Deep-Water Culture (DWC) method. Lettuce plugs were grown for 15 days in controlled environments until two leaves after the cotyledons had developed and were ready for transplant. Plugs were transplanted into a 4 × 6 matrix at the FGCU Food Forest and the DWC growth system. Crops were grown to full bloom and ready for harvest in the soil (60 days) and soilless (30 days) based setups. We collected crop growth data, including wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (μmol/m2). From the collected data, we derived the Specific Leaf Area (SLA, cm2/g) and biomass productivity (kg/m2). Descriptive statistics were used to describe the collected and derived data. We investigated the slopes of regression lines for each growth curve which derived the differences in biomass and productivity parameters between lettuce grown using soil and hydroponics. Both growing methods can grow lettuce crops to full bloom and to adequate harvest weight. The biomass parameters and productivity differ significantly between the growing methods. The lettuce crops grown using hydroponics increase in wet weight statistically and significantly faster than those grown in soil (p < 0.0001). Therefore, we determined that a hydroponic method of crop production may provide better crop output and biomass indicators measured than soil-based growth.

A Comparative Study on the Leaf Characteristics and Root Vigor of Bowl Lotus under Hydroponics and Soil Culture

[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture were set to measure their photosynthetic indices, chlorophyll content and root vigor, and to observe their leaf tissue structure and stomatal characteristics. [Result] The findings indicated that there are no differences in the leaf physiological indices between bowl lotus under hydroponics and soil culture, while the leaf stomata of hydroponic bowl lotus is bigger and its amount is larger than that of soil-culture bowl lotus. At the same time, the ratio of the palisade tissue thickness to spongy tissue thickness is small,and its leaf tissue structure is loose. The root vigor of hydroponic bowl lotus reached its summit earlier, then began to drop. Whereas, the root activity of soil-culture lotus sustained increasing, with vigorous growth. [Conclusion] Therefore, it indicated that hydroponic bowl lotus can adapt to the aquatic-culture environment well and quickly, meanwhile, it also enters into its aging period quickly and its growth cycle gets shorter.

Treatment and utilization of septic tank effluent using vertical-flow constructed wetlands and vegetable hydroponics

Vertical flow constructed wetlands is a typical ecological sanitation system for sewage treatment. The removal rates for COD, BOD 5, SS, TN, and TP were 60%, 80%, 74%, 49% and 79%, respectively, when septic tank effluent was treated by vertical flow filter. So the concentration of COD and BOD\-5 in the treated effluent could meet the quality standard for irrigation water. After that the treated effluent was used for hydroponic cultivation of water spinach and romaine lettuce, the removal efficiencies of the whole system for COD, BOD\-5, SS, TN and TP were 71 4%, 97 5%, 96 9%, 86 3%, and 87 4%, respectively. And it could meet the integrated wastewater discharge standard for secondary biological treatment plant. It was found that using treated effluent for hydroponic cultivation of vegetables could reduce the nitrate content in vegetables. The removal rates for total bacteria and coliform index by using vertical flow bed system with cinder substrate were 80%—90% and 85%—96%, respectively.

Growth Responses of Wheat Cultivars to Rock Phosphate in Hydroponics

Screening cultivars to grow under conditions of low phosphorus (P) availability and utilize P efficiently from compounds of low solubility in soils may be beneficial to overcome poor plant growth in P-deficient soils. The growth behavior and P utilization efficiency of seven wheat cultivars grown in hydroponics were studied, using rock phosphate as P source. The wheat cultivars grown for 30 days were significantly different in biomass accumulation, P uptake and P utilization efficiency. The dry matter production of all the cultivars was significantly correlated with P uptake, which in turn correlated to the drop in the root medium pH. The ranking of wheat cultivars on the basis of dry matter yield, P uptake and P utilization efficiency was Zamindar 80 > Yecora > C 271 > WL 711 > Barani 83 > PARI 73 > Rohtas. The cultivar Zamindar 80 appeared to possess the best growth potential in P-deficient soils.

Response of Lettuce to the Coupling Electrical Conductivity and Circulation Rate of the Nutrient Solution in Hydroponics

The coupling effect of nutrient solution EC （electrical conductivity） levels and CR （circulation rate） on the morphology and quality, in hydroponically grown lettuce was assessed. Lettuce was grown at 5 treatments fi＇om High EC ＆ Low CR to Low EC ＆ High CR. The environmental parameters were controlled in a 20 m^2 plant factory during the hydroponic cultivation with the following values： irradiated by blue and red light-emitting diode lighting with PPFD （photosynthetic photon flux density） value of 150 mol·m^-2·s^-1 for 16 hours per day; Temperature was maintained at 22.0 ℃ during the photoperiod and 16.0 ℃ in dark cycle. The results demonstrate that growing lettuce can be adopted using nutrient solution with lower EC levels and higher CR. The results also indicate that the effect of bi-directional coupled EC and CR resulted in expansion of root length but reducing the root biomass. Nitrate content was significantly reduced.

The Function of Roots of Tea Plant (<i>Camellia sinensis</i>) Cultured by a Novel Form of Hydroponics and Soil Acidification

A novel form of hydroponic culture was employed to explore the physiological function of roots of a tea plant (Camellia sinensis). The pH of the nutrient solution with an actively growing tea plant decreased during cultivation. Furthermore, no oxalic acid, tartaric acid, malic acid or citric acid, all possible factors in acidification, was detected in the nutrient solution of a growing plant. A proton pump inhibitor suppressed the acidification of the solution. Soil acidification might have been accelerated with a proton released from ammoniacal nitrogen preferentially for the growth, suggesting the specific mechanism of tea plant as a functional food.

Characteristics of <i>Morus alba</i>L. Cultured by In-Room Hydroponics

Morus alba L., (cv Ichinose) was cultivated by in-room hydroponics. The flavor and texture of leaves were markedly improved enough to be edible. When the contents of 1-deoxynojirimycin (DNJ) and polyphenols were measured in the hydroponic cultivar, DNJ increased in the leaf compared to the field grown M. alba. However, polyphenols, in contrast, decreased compared to the field cultivar. HPLC profiling revealed marked difference in leaf components between hydroponic and field cultivars indicating relative contents of lipophilic polyphenols were increased. The polyphenols contents, especially, lipophilic polyphenols in the root were remarkably high compared to So-Haku-Hi (Sang Bai Pi in Chinese). The anti-obesity effect of the hydroponically grown Morus was further studied in rats by feeding high-fat, high-sucrose (HFHS) diet with and without supplementation of dried leaf and root powders for 15 weeks. As the result, both the leaf and root from the hydroponic cultivar showed potential anti-obesity and anti-hyperlipidemic functions through amelioration of insulin resistance. Differential effects of leaf and root powders indicated that besides DNJ, the lipophilic polyphenols may play a crucial role in the anti-diabetic function of hydroponically grown Morus alba L. The hydroponics will provide an alternate way to cultivate a novel resource of Morus for developing functional foods and medicines.

Development of a Cylindrical Hydroponics System for Vertical Farming

The study aimed to develop a vertical crop cultivation system for leafy plants based on cylindrical hydroponics and light emitting diode （LED） technology. Investigations were conducted on growing lettuce （Lactuca sativa cv. ＂Rex＂, ＂Nanda＂ and ＂Canasta＂） indoors in a rotary system and Chinese cabbage （Brassica chinensis） in a multi-tier cylindrical hydroponics system under red and blue （RB） LED lightings. Light intensity from different light sources have an influence on the yield and growth behaviour of indoor lettuce. Photosynthetically active radiation （PAR） levels at 63 μmol/m2·s produced low fresh weights （FW） and leaf areas of lettuce ＂Rex＂ and ＂Nanda＂ were grown under rotating conditions. The effect was, however, different on the better developed ＂Canasta＂. Stem etiolation was a common occurrence under such influence. Chlorosis was not observed on all plant types grown under the LEDs. Cultivating Chinese cabbage plants （FW： 28 g/plant） in cylindrical units stacked vertically above another, increased planting density by 47% when compared to the rotary system,

Organic Hydroponics Production

Hydroponic culture is a controlled systems use a soilless growing media,supply all of the plant’s nutrition in the plant’s solutions(water with dissolved fertilizers),result in higher yields of vegetables,flowers,herbs and others crops.Hydroponic systems derive in many various forms and types.Most traditional hydroponic systems are extremely specialized,controlled-environment production systems.Organic hydroponics is a system that is arranged based on organic agriculture of culture.Different approaches are used for controlling of plant pathogens such as physical,chemical,biological controls,biofertilizers,bioremediators and integrated pest management.All the required nutrients are supplied in controlled amounts,including organic crops.This article discuss the way for promoting organic hydroponics systems and to help the small-scale producer make decisions about follow this markets,production methods,and disease control.

Evaluation and Enhanced Use of Light Emitting Diodes for Hydroponics

Hydroponic farming is a viable and economical farming method,which can produce safe and healthy greens and vegetables conveniently and at a relatively low cost.It is essential to provide supplemental lighting for crops grown in greenhouses to meet the daily light requirement,Daily Light Integral(DLI).The present paper investigates how effectively and efficiently LEDs can be used as a light source in hydroponics.It is important for a hydroponic grower to assess the requirement of photo synthetically active radiation(PAR)or the Photosynthetic Photon Flux Density(PPFD),in a greenhouse,and adjust the quality and quantity of supplemental lighting accordingly.A Quantum sensor(or PAR sensor)can measure PAR more accurately than a digital light meter,which measures the light intensity or illuminance in the SI unit Lux,but a PAR sensor is relatively expensive and normally not affordable by an ordinary farmer.Therefore,based on the present investigation and experimental results,a very simple way to convert light intensity measured with a Lux meter into PAR is proposed,using a simple conversion factor(41.75 according to the present work).This allows a small-scale hydroponic farmer to use a simple and inexpensive technique to assess the day to day DLI values of PAR in a greenhouse accurately using just an inexpensive light meter.The present paper also proposes a more efficient way of using LED light panels in a hydroponic system.By moving the LED light panels closer to the crop,LED light source can use a fewer number of LEDs to produce the same required daily light requirement and can increase the efficiency of the power usage to more than 80%.Specifically,the present work has determined that it is important to design more efficient vertically movable LED light panels with capabilities of switching individual LEDs on and off,for the use in greenhouses.This allows a user to control the number of LEDs that can be lit at a particular time,as required.By doing so it is possible to increase the efficiency of a LED lighting system by reducing its cost of the electricity usage.

不同培育方式对橡胶树实生苗生理指标、养分及长势的影响

橡胶树(Hevea brasiliensis)种子催芽生长一般使用沙床培育,沙子是不可再生资源,为了选择一种适合橡胶树种子培育方式来替代对沙子的依赖,该研究通过水培、悬空培育和传统的沙培比较橡胶树实生苗第1蓬叶稳定时,苗木的生长势、生理指标及养分含量。结果表明,水培实生苗地上部株高、茎粗、叶面积的长势最佳,壮苗指数和生物量的含量最高,但其根太长,根相对较细。水培的叶、茎、根的可溶性糖、丙二醛、游离脯氨酸、超氧化物歧化酶的含量均较低;水培和悬空培育的叶片和茎的叶绿素、类胡萝卜素及根系活力的含量没有显著性差异,均高于沙培。水培的叶、茎、根中的氮和磷含量最低,沙培的最高;而水培实生苗根和茎中钾的含量较高,叶片中含量与悬空培育、沙培均没有显著性差异;悬空培育在叶、茎、根中钾的含量最低。水培促进了苗木的生长,降低干旱胁迫,提高养分利用率,但后续还需调控根系,建设良好根团。悬空培育的苗木长势较弱,还需进一步完善方法。

水培大麦苗在畜牧养殖应用的研究进展

大麦籽粒通过水培技术短期培育可得到水培大麦苗,其具有适口性好、易于消化、生长周期短、产出稳定等优点,在畜牧业中可作为青绿饲料,缓解我国畜禽饲料短缺的问题。本文主要对水培大麦苗的生产工艺、营养价值以及其作为饲料资源在畜牧养殖中的应用研究进展进行综述,为水培大麦苗在畜牧生产中的合理应用提供理论参考。

土培和水培条件下两种党参有效成分含量的比较

采用反相高效液相色谱(RP-HPLC)法测定了室内土培和水培条件下渭党和纹党中紫丁香苷、党参炔苷和苍术内酯Ⅲ3种活性成分的含量,以期比较室内不同栽养方式对2种党参有效成分积累水平的影响.色谱流动相采用乙腈(A)-0.3%磷酸水溶液(B),体积流量为1 mL·min^(-1),检测波长为220 nm,柱温为30℃.结果表明,3种受测成分在各自质量浓度范围内线性关系良好(R 2>0.9998),土培纹党中紫丁香苷、苍术内酯Ⅲ含量较高,分别为0.025,0.020 mg·g^(-1);水培渭党中党参炔苷含量较高,为3.944 mg·g^(-1).实验重复性和稳定性良好,精密度和准确性可靠.2种栽养条件下,纹党和渭党中3种活性成分含量差异显著,研究结果可为党参栽培方式的选择和优良品种的筛选提供数据参考.

穗材差异对刺龙牙水培产量的影响

刺龙牙嫩芽味美香甜、清嫩醇厚、野味浓郁,被誉为“山野菜之王”。然而其野生产量远不能满足市场需求,因此,研发一种方便快捷、易于推广且操作性强的刺龙牙栽培技术显得尤为迫切。以辽东山区2种刺龙牙穗材为试材,探讨穗材品种、茎杆长度及梢径对刺龙牙水培生产过程及产量的影响,旨在寻找适合水培生产的刺龙牙穗材指标,提升水培生产效率。结果表明:品种2较品种1开始采摘提前约1.8 d;茎杆长度越长,发芽时间越早,且发芽时间相对集中,从而更早开始采摘,采摘时间更长,采摘次数更多;同样,茎杆越细,发芽时间也越早,且结束发芽时间更早,发芽时间更为集中。此外,刺龙牙穗材水培嫩芽的单根产量达到(18.9±6.2)g,且品种2的水培嫩芽产量高于品种1。当茎杆长度控制在30~60 cm、梢径在10~16 mm范围内时,茎杆长度和梢径均与水培嫩芽产量呈现正相关。

养殖尾水混合不同比例水溶肥种植水培生菜的综合效益评价

以“奶油生菜”为研究对象,将不同量的山崎配方水溶肥溶于工厂化养鱼尾水,配制成混合营养液,进行营养液膜栽培(NFT)水培生菜实验,以期筛选出最佳水肥配比,为实现工厂化养鱼尾水资源化利用提供理论依据。实验设置5个处理,分别为S1(123g水溶肥+150L养鱼尾水)、S2(98.4g水溶肥+150L养鱼尾水)、S3(73.8g水溶肥+150L养鱼尾水)、S4(49.2g水溶肥+150L养鱼尾水)、S5(24.6g水溶肥+150L养鱼尾水),以CK(123g水溶肥+150L清水)作为对照,探究不同处理对生菜产量和品质的影响。采用基于熵权法的TOPSIS综合效益评价模型分析确定最佳水肥配比参数。结果表明,混合营养液能提高生菜对水肥的吸收量,但随水溶肥浓度增加,生菜耗肥量呈先增大后减小的趋势;不同处理下生菜耗水耗肥量均低于CK处理,S1-S5处理相比CK处理分别节肥25.01%、19.15%、34.12%、51.89%和74.10%。混合营养液能有效促进生菜生长,但生菜各项生长指标均随水溶肥浓度上升呈先增大后减少的趋势,S2处理下生菜产量最高,为1.88kg·m^(-2),相比CK处理增加15.32%。此外,混合营养液可提高生菜品质,可溶性糖和可溶性蛋白含量呈先增大后减小的趋势,S2处理下生菜可溶性糖和可溶性蛋白含量最高;维生素C(VC)含量、硝酸盐含量均与水溶肥浓度呈正相关,S1处理下VC含量最高,S1-S5处理下硝酸盐含量均低于CK处理。综合效益评价结果表明,S2处理对生菜的生长最为有利,可以获得较高的产量并兼具较好的品质,为最佳配比,养鱼尾水与水溶肥合理配施后,可显著促进水肥吸收,提高生菜产量和品质,实现作物生产效益最佳。

基于粒子群优化算法的太阳能水培智能控制系统设计与实现

为实现水培营养液水质参数的高效、精确控制,减少设备供能产生的碳排量,构建了一个基于粒子群优化(Particle Swarm Optimization,PSO)算法和最大功率点跟踪(Maximum Power Point Tracking,MPPT)算法的水培智能控制系统。用PSO算法优化模糊控制器的量化、比例因子,加入Smith预估器补偿反馈时延,对pH为4.5、电导率(Electrical Conductivity,EC)为0 mS/cm的营养液进行精确调控。经过优化,分别在44 s和43 s后达到预设值,并能维持稳定状态。建立光伏发电模块,引入MPPT算法,缩短跟踪时长至0.04 s。结果表明,该系统能提高营养液水质参数的调节精度,缩短控制时长,增强水培环境的稳定性;同时,能提升发电效率,实现节能减排。

盐胁迫对水培海巴戟幼苗生理特性的影响

为明确海巴戟幼苗对盐的耐受范围及盐胁迫对海巴戟幼苗生长的影响,以水培180d的海巴戟苗为材料,基本培养基为1/8 MS液体培养基,其中分别加入质量分数为0、0.2%、0.4%、0.6%、0.8%和1.0%的NaCl进行胁迫处理。结果表明:随着NaCl胁迫浓度的增加与胁迫时间的推移,海巴戟叶片相对含水量(relative water content,RWC)与生物量逐渐降低,可溶性蛋白(solubleprotein,SP)、丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)活性均表现为先增加后减少的趋势。海巴戟幼苗的保护酶活性随着NaCl胁迫浓度的增加与胁迫时间的推移发生显著变化,在NaCl浓度0.8%以下范围,海巴戟可以通过调节渗透物质和抗氧化物酶来减轻伤害,保证生长;当NaCl浓度在0.8%及以上范围时,保护酶系统与细胞膜受损严重,进而影响海巴戟生长。因此,海巴戟幼苗可以在0.8%以下NaCl浓度范围的区域试种。

水培条件下花生品种铁素吸收/活化能力鉴定方法

为建立快速鉴定不同花生品种铁素吸收、活化能力的方法,以耐缺铁花生品种农大818、鲁花11、山花11号,铁敏感花生品种鲁花12、ICG6848、白沙1016为材料进行水培试验。以缺铁营养液培养花生幼苗,观察并记录在缺铁环境中幼苗新叶黄化时间;待幼苗新叶全部黄化后恢复供铁,分别以20μmol/L FeSO_(4)处理2 d/4 d,25μmol/L FeSO_(4)处理2 d/3 d,30μmol/L FeSO_(4)处理1 d/2 d/4 d,通过测定叶绿素值增加量(ΔSPAD)、干物重、铁吸收量等指标以鉴定花生铁素吸收能力;以25μmol/L Fe_(2)(SO_(4))_(3)处理2 d/4 d,30μmol/L Fe_(2)(SO_(4))_(3)处理2 d/3 d,35μmol/L Fe_(2)(SO_(4))_(3)处理1 d/2 d/4 d以鉴定花生铁素活化能力。结果表明,所有花生品种的幼苗在缺铁处理第10 d均出现了黄化现象,此时用25μmol/L FeSO_(4)恢复供铁处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁吸收量差异显著;用30μmol/L Fe_(2)(SO_(4))_(3)处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁活化量也有显著差异。因此可在缺铁黄化后补充25μmol/L FeSO_(4)或30μmol/L Fe_(2)(SO_(4))_(3),用处理2 d时花生幼苗ΔSPAD、单株铁吸收量或单株活化量作为评价花生铁素吸收/活化能力的指标。

水培环境下茶树嫩枝扦插的叶绿素荧光特性

【目的】研究水培环境下茶树嫩枝扦插的叶绿素荧光特性。【方法】以福鼎大白茶树嫩枝插穗为材料,先用4种质量浓度(0、50、100、200 mg/L)的萘乙酸(naphthalene acetic acid,NAA)浸泡12 h,再分别对插穗进行水培和土培扦插;采用PAM-2500便携式调制叶绿素荧光仪及生物学统计方法,测定不同质量浓度NAA处理下不同基质培养茶树嫩枝插穗的叶绿素荧光参数和生长发育指标。【结果】茶树嫩枝插穗培养中,89.4%~96.1%的光能被耗散,仅有3.9%~10.6%的光能用于光系统Ⅱ光化学反应。与土培扦插相比,水培嫩枝插穗的可变荧光(F_(v))、潜在光化学活性(F_(v)/F_(o))、光化学猝灭系数(qP)、电子传递速率(electron transport rate,ETR)和愈伤率显著提升,而初始荧光(F_(o))和死亡率显著降低。与水培对照相比,经100 mg/L NAA浸泡的水培插穗,其F_(v)/F_(o)、最大电子传递效率(maximum electron transfer efficiency,ETRmax)、光化学反应[Y(II)]、光响应曲线斜率和愈伤率显著增加,而F_(o)和死亡率显著降低。与土培对照相比,经50 mg/L NAA浸泡的土培插穗,其最大荧光(F_(m))、F_(v)、原初光能转化效率(F_(v)/F_(m))和F_(v)/F_(o)显著降低,生根率显著升高;土培环境下的生根率显著高于水培,其中100和200 mg/L NAA浸泡处理组的插穗生根率均达到30%以上。【结论】茶树嫩枝扦插的光能利用率较低,应避免强光胁迫损伤。水培能显著提升插穗的自我保护能力,增强光合作用能力,加快愈伤组织形成,且不同质量浓度的NAA浸泡处理对水培嫩枝插穗的潜在光化学活性、光启动速率和愈伤率有提升作用,其中插穗前期100 mg/L NAA浸泡12 h处理的效果最佳。研究结果可为茶树嫩枝的规模化水培扦插技术优化提供理论参考。