Erratum to:Vibration analysis of a gear-rotor-bearing system with outer-ring spalling and misalignment

Because of an unfortunate mistake during the production of this article,Figure 13 was wrongly inserted.The corrected Figure 13 is shown as follows.

Analysis and optimization of stamping and forming process of bearing outer ring

To address the common issues of wrinkling,tearing,and uneven wall thickness in the actual sheet metal stamp-ing process of the outer ring of needle roller bearings,this study analyzes critical technical indicators such asforming limits,thickness distribution,and principal strains in the forming process in detail.Three-dimensionalmodels of the concave and convex dies were constructed.The effects of different process parameters,includingstamping speed,edge pressure,sheet metal thickness,and friction coefficient,on the quality of the forming partswere investigated by varying these parameters.Subsequently,the orthogonal experimental method was used todetermine an optimal experimental group from multiple sets of experiments.It was found that under the processparameters of a stamping speed of 3000 mm/s,edge pressure of 2000 N,sheet metal thickness of 0.9 mm,andfriction coefficient of 0.125,the forming quality of the outer ring of the bearing is ideal.

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain unclear.Therefore,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under microgravity.After 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic cells.To investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail suspension.The results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly upregulated.In addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated weightlessness.These findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

Pepper mild mottle virus coat protein interacts with pepper chloroplast outer envelope membrane protein OMP24 to inhibit antiviral immunity in plants

Pepper mild mottle virus(PMMoV)is a devastating viral pathogen of pepper(Capsicum annuum)but it is unclear whether and how peppers protect against PMMoV infection.The expression of the chloroplast outer membrane protein 24(OMP24)of C.annuum was upregulated under PMMoV infection and it interacted with PMMoV coat protein(CP).Silencing of OMP24 in either C.annuum or Nicotiana benthamiana facilitated PMMoV infection,whereas overexpression of N.benthamiana OMP24 in transgenic plants inhibited PMMoV infection.Both C.annuum OMP24(CaOMP24)and N.benthamiana OMP24(NbOMP24)localized to the chloroplast and have a moderately hydrophobic transmembrane domain that is necessary for their localization.Overexpression of CaOMP24 induced stromules,perinuclear chloroplast clustering,and accumulation of reactive oxygen species(ROS),the typical defense responses of chloroplasts transferring the retrograde signaling to the nucleus to regulate resistance genes.The expression of PR1 and PR2 was also upregulated significantly in plants overexpressing OMP24.Self-interaction of OMP24 was demonstrated and was required for OMP24-mediated plant defense.Interaction with PMMoV CP interfered with the self-interaction of OMP24 and impaired OMP24-induced stromules,perinuclear chloroplast clustering and ROS accumulation.The results demonstrate the defense function of OMP24 in pepper during viral infection and suggest a possible mechanism by which PMMoV CP modulates the plant defense to facilitate viral infection.

A data assimilation-based forecast model of outer radiation belt electron fluxes

Because radiation belt electrons can pose a potential threat to the safety of satellites orbiting in space,it is of great importance to develop a reliable model that can predict the highly dynamic variations in outer radiation belt electron fluxes.In the present study,we develop a forecast model of radiation belt electron fluxes based on the data assimilation method,in terms of Van Allen Probe measurements combined with three-dimensional radiation belt numerical simulations.Our forecast model can cover the entire outer radiation belt with a high temporal resolution(1 hour)and a spatial resolution of 0.25 L over a wide range of both electron energy(0.1-5.0 MeV)and pitch angle(5°-90°).On the basis of this model,we forecast hourly electron fluxes for the next 1,2,and 3 days during an intense geomagnetic storm and evaluate the corresponding prediction performance.Our model can reasonably predict the stormtime evolution of radiation belt electrons with high prediction efficiency(up to~0.8-1).The best prediction performance is found for~0.3-3 MeV electrons at L=~3.25-4.5,which extends to higher L and lower energies with increasing pitch angle.Our results demonstrate that the forecast model developed can be a powerful tool to predict the spatiotemporal changes in outer radiation belt electron fluxes,and the model has both scientific significance and practical implications.

Newly Detected Transmission of bla_(KPC-2) by Outer Membrane Vesicles in Klebsiella Pneumoniae

Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins and other factors involved in various biological processes,includingβ-lactamase and antibiotic-resistance genes.This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella(K.)pneumoniae.Methods We selected CR-KP producing K.pneumoniae carbapenemase-2(KPC-2)to study whether they can transfer resistance genes through OMVs.The OMVs of CR-KP were obtained by ultracentrifugation,and incubated with carbapenem-sensitive K.pneumoniae for 4 h.Finally,the carbapenem-sensitive K.pneumoniae was tested for the presence of bla_(KPC-2)resistance gene and its sensitivity to carbapenem antibiotics.Results The existence of OMVs was observed by the electron microscopy.The extracted OMVs had bla_(KPC-2)resistance gene.After incubation with OMVs,bla_(KPC-2)resistance gene was detected in sensitive K.pneumoniae,and it became resistant to imipenem and meropenem.Conclusion This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver bla_(KPC-2)to sensitive K.pneumoniae,allowing the bacteria to produce carbapenemase,which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.

A Lagrangian Trajectory Analysis of Azimuthally Asymmetric Equivalent Potential Temperature in the Outer Core of Sheared Tropical Cyclones

In this study,the characteristics of azimuthally asymmetric equivalent potential temperature(θ_(e))distributions in the outer core of tropical cyclones(TCs)encountering weak and strong vertical wind shear are examined using a Lagrangian trajectory method.Evaporatively forced downdrafts in the outer rainbands can transport low-entropy air downward,resulting in the lowestθ_(e)in the downshear-left boundary layer.Quantitative estimations ofθ_(e)recovery indicate that air parcels,especially those originating from the downshear-left outer core,can gradually revive from a low entropy state through surface enthalpy fluxes as the parcels move cyclonically.As a result,the maximumθ_(e)is observed in the downshear-right quadrant of a highly sheared TC.The trajectory analyses also indicate that parcels that move upward in the outer rainbands and those that travel through the inner core due to shear make a dominant contribution to the midlevel enhancement ofθ_(e)in the downshear-left outer core.In particular,the former plays a leading role in suchθ_(e)enhancements,while the latter plays a secondary role.As a result,moist potential stability occurs in the middle-to-lower troposphere in the downshear-left outer core.

Proteomic and Functional Analyses of Outer Membrane Vesicles Secreted by Vibrio splendidus

Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured animals.The extracellular products secreted by V.splendidus are crucial to bacterial survival and virulence.In this study,the secretion of outer membrane vesicles(OMVs)by V.splendidus was determined,purified,and morphologically characterized.The protein composition of OMVs was analyzed by proteomic analysis.The results showed that approximately 120 proteins were contained in these OMVs,including outer membrane proteins,flagellins,ABC transporters,protease,and iron regulation proteins,etc.,which were involved in bacterial motility,formation of biofilms and the cell membrane components,and cellular localization based on their structural molecule activity,passive transmembrane transporter activity,channel activity,neurotransmitter receptor activity,extracellular ligand-gated ion channel activity,glutamate receptor activity,ligand-gated ion channel activity,and transmembrane signaling receptor activity.To explore the biological functions of OMVs in V.splendidus,the effects of OMVs on the bacterial adaption to iron limitation,antibiotic,and the coelomic fluid of the Apostichopus japonicus were confirmed.This study is the first time to show that V.splendidus secretes OMVs,and OMVs carry functional proteins that enhance bacterial survival under various stresses.

On-Site Assessment of a Cryogenic Disinfectant for the Alpine Environment and Outer Packaging of Frozen Items

Objective To study the effectiveness and feasibility of cryogenic disinfectants in different cold scenarios and analyze the key points of on-site cryogenic disinfection.Methods Qingdao and Suifenhe were selected as application sites for the manual or mechanical spraying of cryogenic disinfectants.The same amount of disinfectant(3,000 mg/L)was applied on cold chain food packaging,cold chain containers,transport vehicles,alpine environments,and article surfaces.The killing log value of the cryogenic disinfectant against the indicator microorganisms(Staphylococcus aureus and Escherichia coli)was used to evaluate the on-site disinfection effect.Results When using 3,000 mg/L with an action time of 10 min on the ground in alpine regions,the surface of frozen items,cold-chain containers,and cold chain food packaging in supermarkets,all external surfaces were successfully disinfected,with a pass rate of 100%.The disinfection pass rates for cold chain food packaging and cold chain transport vehicles of centralized supervised warehouses and food processing enterprises were 12.5%(15/120),81.67%(49/60),and 93.33%(14/15),respectively;yet,the surfaces were not fully sprayed.Conclusion Cryogenic disinfectants are effective in disinfecting alpine environments and the outer packaging of frozen items.The application of cryogenic disinfectants should be regulated to ensure that they cover all surfaces of the disinfected object,thus ensuring effective cryogenic disinfection.

Effect of High-Speed Solar Winds Turbulence Upstream of the Earth’s Magnetosphere: Case of the Outer Minima of Solar Cycles 20, 21, 22, 23 and 24

Highly turbulent environment, the solar wind is a stream of very energetic particles mainly made of protons and electrons. During its trip in the interplanetary space, this solar flow becomes more accelerated during the outer minima (descending phases) of the solar cycles and can therefore influence all of humanity and its technology. These disturbances lead to socio-economic consequences requiring a precise knowledge of the climate variability. Using a statistical approach, we evaluate the response of the Earth’s magnetosphere to the High-Speed Solar Winds (HSSW) forcing during the peaks of the last five outer minima. To do so, 1UA data of solar wind and magnetic field parameters were extracted from OMNI browser. Analysis of the energetic solar plasma particles shows that strong geomagnetic field variations can occur even in the absence of large solar disturbances. While the normalized reconnection rate was estimated to be ~21% of the total variance of the magnetospheric variables, the upstream of the magnetic cavity was perturbed 80% of the time with large energies recorded. As a result, Earth’s magnetosphere becomes denser (i.e., more drag), which is a problem for spacecraft. Thus, the coupled solar wind-magnetosphere system follows scale-invariant dynamics and is in a state far from equilibrium. Our analysis provides insight into the main cause of geomagnetic storms with more than 97% of HSSW imposed in the range 300 - 850 km/s. These high-speeds lead to auroras that can disrupt electrical and communication systems.

RELEASE KINETICS OF MONOLITHIC MATRIX COATED WITH OUTER MEMBRANE SYSTEM

The reservoir-monolithic type of the controlled release systems is investigated currently,however,the existing kinetic model could not describe the release process well because the release kinetics is rather complicated.In this paper,a simplified release kinetic model for diffusion-controlled monolithic matrix coated with outer membrane systems is proposed and verified by the experimental data of mercaptopurinum release experiment.It shows that the model can well describe the release mechanism (the relative error is under 3%) when drug loading (C d) is above its solubility limit (C s).At the same time,the release characteristics of special cases (D mD f and D mD f) are discussed theoretically.When D mD f the release rate becomes constant,namely,zero order release,and the release rate is independent of the drug membrane.This result provides the theoretical basis for the system of zero order release as well as how to control the release rate and the amount of drug release.When D mD f,the release rate is dependent on the drug release coefficient in the monolithic matrix,solubility and drug loading but independent of the process in the outer membrane,and it is similar to monolithic matrix type.

Malfunction of outer retinal barrier and choroid in the occurrence and progression of diabetic macular edema

Diabetic macular edema(DME) is the most common cause of vision loss in diabetic retinopathy,affecting 1 in 15 patients with diabetes mellitus(DM).The disruption of the inner blood-retina barrier(BRB) has been largely investigated and attributed the primary role in the pathogenesis and progression in DME, but there is increasing evidence regarding the role of outer BRB, separating the RPE from the underlying choriocapillaris,in the occurrence and evolution of DME.The development of novel imaging technologies has led to major improvement in the field of in vivo structural analysis of the macula allowing us to delve deeper into the pathogenesis of DME and expanding our vision regarding this condition.In this review we gathered the results of studies that investigated specific outer BRB optical coherence tomography parameters in patients with DM with the aim to outline the current status of its role in the pathogenesis and progression of DME and identify new research pathways contributing to the advancement of knowledge in the understanding of this condition.

Earth's deformation due to the dynamical perturbations of the fluid outer core

The elasto-gravitational deformation response of the Earths solid parts to the perturbations of the pressure and gravity on the core-mantle boundary (CMB) and the solid inner core boundary (ICB), due to the dynamical behaviors of the fluid outer core (FOC), is discussed. The internal load Love numbers, which are formulized in a general form in this study, are employed to describe the Earths deformation. The preliminary reference Earth model (PREM) is used as an example to calculate the internal load Love numbers on the Earths surface, CMB and ICB, respectively. The characteristics of the Earths deformation variation with the depth and the perturbation periods on the boundaries of the FOC are also investigated. The numerical results indicate that the internal load Love numbers decrease quickly with the increasing degree of the spherical harmonics of the displacement and depend strongly on the perturbation frequencies, especially on the high frequencies. The results, obtained in this work, can be used to construct the boundary conditions for the core dynamics of the long-period oscillations of the Earths fluid outer core.

Study on Outer Membrane Protein Patterns of Escherichia coli O38,O53 and O75 Isolated from Chickens

[Objective] This study aimed to investigate the outer membrane protein （OMP） patterns of Escherichia coli 038, 053 and 075 isolates from chickens. [Method] Eight pathogenic E. coil isolates with various serotypes were used as experimental materials to extract OMP by using supersonic schizolysis method and Sarcosyl. After SDS-PAGE electrophoresis, OMP patterns of the extracted products were determined based on the OMP model diagram. [Result] OMP of eight E. coil isolates with three serotypes were divided into three patterns, to be specific, 2 075 isolates respectively belonged to OMP-I and OMP-II pattern, 1 053 isolate belonged to OMP-II pattern, and 5 038 isolates belonged to OMP-I and OMP-III pattern. [Conclusion] Experimental results showed that E. coli isolates with the same serotype may belong to completely different OMP patterns, while serologically unrelated isolates may belong to the same OMP pattern. OMP of E. coil isolates with the same serotype may generate genetic differentiation; in addition, OMP of E. coli isolates with different serotypes may have different genetic correlation.

Prokaryotic Expression and Identification of Outer Membrane Protein 2 of Chlamydia trachomatis

Objective: To construct a recombinant plasmid containing the outer membrane protein 2 (Omp2) gene of Chlamydia trachomatis and express Omp2 in E.coli. Methods: The omp2 gene of C. trachomatis serovar D was cloned into pQE30 vector following PCR amplification from genomic DNA. E. coli M15 transformants were induced to express the fusion protein by IPTG and the product was identified by SDS-PAGE and Western blot. Results: Confirmed by enzyme cleavage analysis and DNA sequencing, a correct recombinant plasmid pQE30/omp2 was constructed. The fusion protein from the transformants was approximately 60 kDa in size in SDS-PAGE analysis, which could specially react with anti-6 X His mouse monoclonal IgG antibodies. Conclusion: We successfully expressed Omp2 in E. coli M15, providing an efficient and simple system for assaying the immunological properties of Omp2.

Effects of Semi-floating Ring Bearing Outer Clearance on the Subsynchronous Oscillation of Turbocharger Rotor

Semi-floating ring bearing（SFRB） is developed to control the vibration of turbocharger rotor. The outer clearance of SFRB affects the magnitude and frequency of nonlinear whirl motion, which is significant for the design of turbocharger. In order to explore the effects of outer clearance, a transient finite element analysis program for rotor and oil film bearing is built and validated by a published experimental case. The nonlinear dynamic behaviors ofrotor-SFRB system are simulated. According to the simulation results, two representative subsynchronous oscillations excited by the two hearings respectively are discovered. As the outer clearance of SFRB increases from 24 ~tm to 60 pro, the low-frequency subsynchronous oscillation experiences three steps, including a strong start, a gradual recession and a combination with the other one. At the same time, the high-frequency subsynchronous oscillation starts to appear gradually, then strengthens, and finally combines. If gravity and unbalance are neglected, the combination will start starts from high rotor speed and extents to low rotor speed, just like a ＂zipper＂. It is found from the quantitative analysis that when the outer clearance increases, the vibration amplitude experiences large value firstly, then reduction, and suddenly increasing after combination. A useful design principle of SFRB outer clearance for minimum vibration amplitude is proposed： the outer clearance value should be chosen to keep the frequency of two subsynchronous oscillations clearly separated and their amplitudes close.

Characteristics of the Outer Rainband Stratiform Sector in Numerically Simulated Tropical Cyclones:Lower-Layer Shear versus Upper-Layer Shear

Idealized numerical simulations are conducted in this study to comparatively investigate the characteristics of the stratiform sector in the outer rainbands of tropical cyclones(TCs)in lower-and upper-layer vertical wind shear(VWS)with moderate magnitude.Consistent with the results in previous studies,the outer rainband stratiform sector of the TCs simulated in both experiments is generally located downshear left.Upper-layer VWS tends to produce stronger asymmetric outflow at upper levels in the downshear-left quadrant than lower-layer shear.This stronger asymmetric outflow transports more water vapor radially outward from the inner core to the outer core at upper levels in the downshear-left quadrant in the upper-layer shear experiment.More depositional growth of both graupel and cloud ice thus occurs downshear left in upper layers in the outer core,yielding more diabatic heating and stronger upward motions,particularly in the stratiformdominated part of the stratiform sector in the upper-layer shear experiment.Resultingly,a better-organized stratiform sector in the outer rainbands is found in the upper-layer VWS experiment than in the lower-layer VWS experiment.The diabatic heating associated with the stratiform sector produces strong midlevel outflow on the radially inward side of,and weak midlevel inflow on the radially outward side of,the heating core,with lower-level inflow beneath the midlevel outflow and upper-level inflow above.The upper-layer VWS tends to produce a deeper asymmetric inflow layer in the outer rainband stratiform sector,with more significant lower-level inflow and tangential jets in the upper-layer VWS experiment.

DS-CDMA system outer loop power control and improvement for multi-service

When a new user accesses the CDMA system, the load will change drastically, and therefore, the advanced outer loop power control （OLPC） technology has to be adopted to enrich the target signal interference ratio （Silt） and improve the system performance. The existing problems about DS-CDMA outer loop power control for multi-service are introduced and the power control theoretical model is analyzed. System simulation is adopted on how to obtain the theoretical performance and parameter optimization of the power control algorithm. The OLPC algorithm is improved and the performance comparisons between the old algorithm and the improved algorithm are given. The results show good performance of the improved OLPC algorithm and prove the validity of the improved method for multi-service.

A Mathematical Model for Diffusion-Controlled Monolithic Matrix Coated with outer Membrane System

A release model for diffusion-controlled monolithic matrix coated with outer membrane system is proposed and solved by using the refined double integral method. The calculated results are in satisfactory agreement with the experimental release data. The present model can be well used to describe the release process for all cd/cs values. In addition, the release effects of the monolithic matrix coated with outer membrane system are discussed theoretically.

Diversity of metabolite accumulation patterns in inner and outer seed coats of pomegranate:exploring their relationship with genetic mechanisms of seed coat development

The expanded outer seed coat and the rigid inner seed coat of pomegranate seeds,both affect the sensory qualities of the fruit and its acceptability to consumers.Pomegranate seeds are also an appealing model for the study of seed coat differentiation and development.We conducted nontarget metabolic profiling to detect metabolites that contribute to the morphological differentiation of the seed coats along with transcriptomic profiling to unravel the genetic mechanisms underlying this process.Comparisons of metabolites in the lignin biosynthetic pathway accumulating in seed coat layers at different developmental stages revealed that monolignols,including coniferyl alcohol and sinapyl alcohol,greatly accumulated in inner seed coats and monolignol glucosides greatly accumulated in outer seed coats.Strong expression of genes involved in monolignol biosynthesis and transport might explain the spatial patterns of biosynthesis and accumulation of these metabolites.Hemicellulose constituents and flavonoids in particular accumulated in the inner seed coat,and candidate genes that might be involved in their accumulation were also identified.Genes encoding transcription factors regulating monolignol,cellulose,and hemicellulose metabolism were chosen by coexpression analysis.These results provide insights into metabolic factors influencing seed coat differentiation and a reference for studying seed coat developmental biology and pomegranate genetic improvement.