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棉花GhPIF4调控高温下花药败育机制初探 被引量:6
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作者 陈淼 谢赛 +3 位作者 王超智 李焱龙 张献龙 闵玲 《作物学报》 CAS CSCD 北大核心 2020年第9期1368-1379,共12页
PIF4属于PIF家族(PHYTOCHROME INTERACTING FACTORS FAMILY),是一个响应温度变化的关键性转录因子,广泛参与植物热信号传导和激素信号通路。本研究从陆地棉‘YZ1’中克隆获得GhPIF4基因,亚细胞定位结果表明,GhPIF4是一个核蛋白。qRT-PC... PIF4属于PIF家族(PHYTOCHROME INTERACTING FACTORS FAMILY),是一个响应温度变化的关键性转录因子,广泛参与植物热信号传导和激素信号通路。本研究从陆地棉‘YZ1’中克隆获得GhPIF4基因,亚细胞定位结果表明,GhPIF4是一个核蛋白。qRT-PCR分析和proGhPIF4:GUS转基因棉花GUS染色结果显示,GhPIF4基因在棉花花药中高量表达,且其表达在高温敏感型材料‘H05’中受到高温的显著诱导。在棉花中超量表达GhPIF4,不同的转基因株系表现不同的育性,表达量高的3个转基因系(OE5、OE7和OE19)开花当天的花药不开裂,花粉的活力均低于对照‘YZ1’,而超表达程度略低的OE10,花药正常开裂,花粉活性正常,表明GhPIF4导致的花药败育具有剂量效应。对OE7和OE19在9~14 mm(绒毡层降解期)、14~19 mm(有丝分裂I期)中生长素的含量以及生长素合成关键基因GhTAA1、GhYUC2、GhCYP71A13的表达分析发现基因的表达与生长素含量下降变化一致。推测超表达GhPIF4在花药后期可能部分模拟棉花的高温响应状态,而组成型超表达GhPIF4可能改变了营养器官或早期花药中生长素的含量,亦或花药中生长素含量过低也会导致花药败育。以上结果为深入解析GhPIF4基因功能及了解高温胁迫下棉花花药败育的机制提供参考。 展开更多
关键词 棉花 高温 pif4 生长素 花药/花粉败育
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拟南芥热形态建成中PIF4下游基因研究 被引量:5
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作者 汪硕 丁岚 +1 位作者 刘建祥 韩佳嘉 《生物技术通报》 CAS CSCD 北大核心 2018年第7期57-65,共9页
植物细胞伸长是植物生长和形态建成的主要方式之一,受到激素和环境信号等许多因素调控,而光敏色素PHY B互作蛋白PIF4可以促进植物在环境温度升高后的形态建成即热形态建成,但PIF4在这个响应过程中全基因组水平调控的下游基因未见报道。... 植物细胞伸长是植物生长和形态建成的主要方式之一,受到激素和环境信号等许多因素调控,而光敏色素PHY B互作蛋白PIF4可以促进植物在环境温度升高后的形态建成即热形态建成,但PIF4在这个响应过程中全基因组水平调控的下游基因未见报道。本研究进一步通过遗传实验证明PIF4在热形态建成中发挥着重要的作用。通过RNA-Seq实验分析得到热形态建成中248个PIF4依赖的和21个PIF4部分依赖的下游基因,包括生长素信号通路和非生物逆境响应基因。结合已有CHIP-Seq数据得到74个热形态建成中PIF4结合靶标基因。拟南芥热形态建成过程中PIF4下游基因的研究为更好理解植物对环境温度升高的响应奠定了基础。 展开更多
关键词 环境温度升高 下胚轴伸长 pif4 下游基因 RNA-SEQ CH IP-q PCR
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The BHLH Transcriptional Factor PIF4 Competes with the R2R3-MYB Transcriptional Factor MYB75 to Fine-Tune Seeds Germination under High Glucose Stress 被引量:2
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作者 Xiaoli Li Shiyan Lu +4 位作者 Yaru Yang Wenjie Wei Jiali Wei Xiaojun Yuan Ping Li 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第5期1387-1400,共14页
It is known that the high level of sugar including glucose suppresses seed germination through ABA signal.ABI5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism ne... It is known that the high level of sugar including glucose suppresses seed germination through ABA signal.ABI5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism needs more investigation.Previous study demonstrated the PIF4 activated the expression of ABI5 to suppress seed germination in darkness.Here we reported that PIF4 also mediated the seed germination inhibition through ABI5 under high concentration of glucose treatment.Furthermore,we found that PIF4 interacted with PAP1,the central factor to control anthocyanin biosynthesis.Such interaction was confirmed in vitro and in planta.Biochemical and physiological analysis revealed that PAP1 bond the promoter of ABI5 to suppress its expression,thus enhanced seed germination under high concentration of glucose treatment.Specially,PAP1 competed with PIF4 to antagonize the activation of PIF4 on ABI5 expression,thus promoted seed germination under high glucose treatment.Given these,we uncover a novel role for PIF4 and PAP1 in controlling seed germination under high glucose treatment,and reveal their antagonistic mechanism by which coordinates ABI5 expression to control seed germination in response to the glucose signal. 展开更多
关键词 Seeds germination GLUCOSE MYB75 pif4 ABI5
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HY5通过PIF4基因控制高温诱导的拟南芥下胚轴伸长反应 被引量:2
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作者 郑兰兰 李琛 +3 位作者 张璟璇 兰红梦 周蒙 张勇洪 《湖北农业科学》 2017年第18期3554-3558,共5页
考察了拟南芥野生型、hy5、pif4及hy5pif4双突变体在22℃和30℃培养下的下胚轴伸长表型;利用q RT-PCR监测PIF4基因在野生型和hy5突变体30℃处理后持续多个时间点的动态表达。结果表明,HY5通过抑制下游基因PIF4调控高温诱导的下胚轴伸长... 考察了拟南芥野生型、hy5、pif4及hy5pif4双突变体在22℃和30℃培养下的下胚轴伸长表型;利用q RT-PCR监测PIF4基因在野生型和hy5突变体30℃处理后持续多个时间点的动态表达。结果表明,HY5通过抑制下游基因PIF4调控高温诱导的下胚轴伸长。PIF4::GUS启动子融合报告基因株系的染色结果表明,高温对PIF4转录水平的诱导主要发生在子叶而不是下胚轴。通过q RT-PCR检测PIF4同源基因PIF5,生长素合成基因YUC2、YUC3、YUC8和生长素反应基因IAA29在野生型和hy5突变体的动态表达,HY5通过调控PIF4介导的生长素通路来控制拟南芥下胚轴的伸长反应。 展开更多
关键词 拟南芥(Arabidopsis thaliana) HY5 pif4 生长素 高温 下胚轴
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温度形态建成中关键转录因子PIF4所受调控的研究进展
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作者 张敏 苏立新 王昕 《安徽农业科学》 CAS 2021年第20期22-24,35,共4页
温度形态建成是非逆境高温引起的植物的一系列形态变化,是植物适应高温环境的重要机制。PIF4是温度形态建成的正向调控因子,在温度形态建成中发挥着非常重要的调控作用。因此在长期的自然选择过程中,植物进化出了复杂的调控PIF4活性的... 温度形态建成是非逆境高温引起的植物的一系列形态变化,是植物适应高温环境的重要机制。PIF4是温度形态建成的正向调控因子,在温度形态建成中发挥着非常重要的调控作用。因此在长期的自然选择过程中,植物进化出了复杂的调控PIF4活性的分子机制。将从转录、蛋白质稳定性、与靶序列的结合强度和调控转录的活性4个方面来阐述调控PIF4活性的分子机制,并讨论了今后可能的研究方向。 展开更多
关键词 温度形态建成 pif4 转录水平调控 蛋白质水平调控
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PIF4整合植物的光照和温度信号转导通路
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作者 张婉月 王鹤潼 王昕 《中文科技期刊数据库(全文版)自然科学》 2022年第8期283-285,共3页
作为固着生长的生物,植物无法通过移动来躲避不利的环境条件。因此,它们进化出了复杂的信号转导系统来根据环境条件的变化调控自身的生长发育。光敏色素互作因子4(PHYTOCHROME-INTERCTING FACTOR 4, PIF4)是光信号转导途径中的重要调控... 作为固着生长的生物,植物无法通过移动来躲避不利的环境条件。因此,它们进化出了复杂的信号转导系统来根据环境条件的变化调控自身的生长发育。光敏色素互作因子4(PHYTOCHROME-INTERCTING FACTOR 4, PIF4)是光信号转导途径中的重要调控因子。接下来,PIF4又被鉴定为温度信号转导途径中的重要调控因子。因此,PIF4被认为是整合光信号和温度信号的分子枢纽。本文综述了PIF4在光信号和温度信号转导通路中发挥的功能和PIF4在整合这两条通路过程中的作用。此外,我们还讨论了PIF4研究中尚未被回答的问题以及未来可能的研究方向。 展开更多
关键词 pif4转录因子 温度信号转导途径 光信号转导途径
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PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling 被引量:2
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作者 Xiaofeng Luo Yujia Dai +15 位作者 Baoshan Xian Jiahui Xu Ranran Zhang Muhammad Saad Rehmani Chuan Zheng Xiaoting Zhao Kaitao Mao Xiaotong Ren Shaowei Wei Lei Wang Juan He Weiming Tan Junbo Du Weiguo Liu Shu Yuan Kai Shu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第5期909-927,共19页
Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been documented.However,the mechanisms underlying the interactions between different TFs within a tra... Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been documented.However,the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely unknown.Here,we showed that TF PHYTOCHROME-INTERACTING FACTOR4(PIF4)physically interacted with the abscisic acid(ABA)signaling responsive TF ABSCISIC ACID INSENSITIVE4(ABI4)to act as a transcriptional complex to promote ABA biosynthesis and signaling,finally deepening primary seed dormancy.Both pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype,with a synergistic effect in the pif4/abi4 double mutant.PIF4 binds to ABI4 to form a heterodimer,and ABI4 stabilizes PIF4 at the protein level,whereas PIF4 does not affect the protein stabilization of ABI4.Subsequently,both TFs independently and synergistically promoted the expression of ABI4 and NCED6,a key gene for ABA anabolism.The genetic evidence is also consistent with the phenotypic,physiological and biochemical analysis results.Altogether,this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling. 展开更多
关键词 ABA ABI4 pif4 seed dormancy and germination transcriptional complex
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Near-infrared light and PIF4 promote plant antiviral defense by enhancing RNA interference 被引量:1
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作者 Xuan Zhang Duan Wang +3 位作者 Pingzhi Zhao Yanwei Sun Rong-Xiang Fang Jian Ye 《Plant Communications》 SCIE CSCD 2024年第1期41-54,共14页
The molecular mechanism underlying phototherapy and light treatment,which utilize various wavelength spectra of light,including near-infrared(NIR),to cure human and plant diseases,is obscure.Here we re-vealed that NIR... The molecular mechanism underlying phototherapy and light treatment,which utilize various wavelength spectra of light,including near-infrared(NIR),to cure human and plant diseases,is obscure.Here we re-vealed that NIR light confers antiviral immunity by positively regulating PHYTOCHROME-INTERACTING FACTOR 4(PIF4)-activated RNA interference(RNAi)in plants.PIF4,a central transcription factor involved in light signaling,accumulates to high levels under NIR light in plants.PIF4 directly induces the transcription of two essential components of RNAi,RNA-DEPENDENT RNA POLYMERASE 6(RDR6)and ARGONAUTE 1(AGO1),which play important roles in resistance to both DNA and RNA viruses.Moreover,the pathogenic determinant bC1 protein,which is evolutionarily conserved and encoded by betasatellites,interacts with PIF4 and inhibits its positive regulation of RNAi by disrupting PIF4 dimerization.Thesefindings shed light on the molecular mechanism of PIF4-mediated plant defense and provide a new perspective for the explo-ration of NIR antiviral treatment. 展开更多
关键词 light NEAR-INFRARED pif4 RNAI plant antiviral immunity
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Regulation of cryptochrome-mediated blue light signaling by the ABI4–PIF4 module
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作者 Pengyu Song Zidan Yang +5 位作者 Huaichang Wang Fangfang Wan Dingming Kang Wenming Zheng Zhizhong Gong Jigang Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第11期2412-2430,共19页
ABSCISIC ACID-INSENSITIVE 4(ABI4) is a pivotal transcription factor which coordinates multiple aspects of plant growth and development as well as plant responses to environmental stresses.ABI4has been shown to be invo... ABSCISIC ACID-INSENSITIVE 4(ABI4) is a pivotal transcription factor which coordinates multiple aspects of plant growth and development as well as plant responses to environmental stresses.ABI4has been shown to be involved in regulating seedling photomorphogenesis;however,the underlying mechanism remains elusive.Here,we show that the role of ABI4 in regulating photomorphogenesis is generally regulated by sucrose,but ABI4 promotes hypocotyl elongation of Arabidopsis seedlings under blue(B) light under all tested sucrose concentrations.We further show that ABI4 physically interacts with PHYTOCHROME INTERACTING FACTOR 4(PIF4),a well-characterized growth-promoting transcription factor,and post-translationally promotes PIF4 protein accumulation under B light.Further analyses indicate that ABI4 directly interacts with the B light photoreceptors cryptochromes(CRYs) and inhibits the interactions between CRYs and PIF4,thus relieving CRY-mediated repression of PIF4 protein accumulation.In addition,while ABI4 could directly activate its own expression,CRYs enhance,whereas PIF4 inhibits,ABI4-mediated activation of the ABI4 promoter.Together,our study demonstrates that the ABI4–PIF4 module plays an important role in mediating CRY-induced B light signaling in Arabidopsis. 展开更多
关键词 ABI4 Arabidopsis thaliana CRYs PHOTOMORPHOGENESIS pif4
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A warm temperature-released negative feedback loop fine-tunes PIF4-mediated thermomorphogenesis in Arabidopsis
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作者 Hui Li Mande Xue +4 位作者 Huairen Zhang Fengyue Zhao Xiaoyi Li Shuancang Yu Danhua Jiang 《Plant Communications》 SCIE CSCD 2024年第5期208-224,共17页
Plants can sense temperature changes and adjust their growth accordingly.In Arabidopsis,high ambient temperatures stimulate stem elongation by activating a key thermoresponsive regulator,PHYTOCHROME INTERACTING FACTOR... Plants can sense temperature changes and adjust their growth accordingly.In Arabidopsis,high ambient temperatures stimulate stem elongation by activating a key thermoresponsive regulator,PHYTOCHROME INTERACTING FACTOR 4(PIF4).Here,we show that warmth promotes the nighttime transcription of GI,which is necessary for the high temperature-induced transcription of TOC1.Genetic analyses suggest that GI prevents excessive thermoresponsive growth by inhibiting PIF4,with this regulatory mechanism be-ing partially reliant on TOC1.GI transcription is repressed by ELF3 and HY5,which concurrently inhibit PIF4 expression and activity.Temperature elevation causes the deactivation or degradation of ELF3 and HY5,leading to PIF4 activation and relief of GI transcriptional repression at high temperatures.This allows PIF4 to further activate GI transcription in response to elevated temperatures.GI,in turn,inhibits PIF4,es-tablishing a negative feedback loop thatfine-tunes PIF4 activity.In addition,we demonstrate that ELF3,HY5,and PIF4 regulate GI transcription by modulating the enrichment of histone variant H2A.Z at the GI lo-cus.Together,ourfindings suggest that thermal release of a negative feedback loopfinely adjusts plant thermomorphogenesis. 展开更多
关键词 TEMPERATURE thermomorphogenesis pif4 GI TOC1 feedback loop
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PIF4转录因子调控植物热形态建成研究进展 被引量:3
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作者 赵小刚 隋心意 温祥珍 《植物生理学报》 CAS CSCD 北大核心 2022年第3期492-500,共9页
热形态建成是植物在长期进化中产生的适应性性状。叶柄和节间伸长、叶片偏上生长有助于增强蒸腾作用,提高植物对高温的适应性。研究表明,光敏色素互作因子PIF4通过整合光照、昼夜节律和激素信号调控植物热形态建成。本文从这三方面介绍... 热形态建成是植物在长期进化中产生的适应性性状。叶柄和节间伸长、叶片偏上生长有助于增强蒸腾作用,提高植物对高温的适应性。研究表明,光敏色素互作因子PIF4通过整合光照、昼夜节律和激素信号调控植物热形态建成。本文从这三方面介绍了近年来国内外对于PIF4在热形态建成方面的研究进展,旨在为后续工作提供一些提供参考。此外,本文也提出了本领域需要进一步研究的科学问题,试图为解释PIF4调控热形态建成的分子机理提供新的研究信息。 展开更多
关键词 热形态建成 pif4 光照 昼夜节律 激素
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SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth 被引量:16
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作者 Junling Huai Xinyu Zhang +4 位作者 dialong Li Tingting Ma Ping Zha Yanjun Jing Rongcheng Lin 《Molecular Plant》 SCIE CAS CSCD 2018年第7期928-942,共15页
Plants continuously monitor environmental conditions (such as light and temperature) and adjust their growth and development accordingly. The transcription factor PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates b... Plants continuously monitor environmental conditions (such as light and temperature) and adjust their growth and development accordingly. The transcription factor PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates both light and temperature signaling pathways. Here, we identified ENHANCED PHOTOMORPHOGENIC2 (EPP2) as a new repressor of photomorphogenesis in red, far-red, and blue light. Map-based cloning revealed that EPP2 encodes the SEUSS (SEU) transcription regulator. The C terminus of SEU has transcriptional activation activity, and SEU physically interacts with PIF4. Moreover, SEU promotes the expression of many genes, including auxin biosynthetic and responsive genes, and regulates IAA levels in plants. SEU associates with the regulatory regions of INDOLE-3-ACETIC ACID INDUCIBLE6 (IAA6) and IAA 19 in a PIF4-independent manner, whereas the binding of PIF4to these genes requires SEU. Furthermore, muta- tions in SEU affect H3K4me3 methylation at IAA6 and IAA 19, and SEU positively regulates warm temperature- mediated hypocotyl growth together with PIF4. Collectively, our results reveal that SEU acts as a central regulator integrating light and temperature signals to control plant growth by coordinating with PIF4. 展开更多
关键词 LIGHT TEMPERATURE SEU pif4 transcription regulation
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HOS1 Facilitates the Phytochrome B-Mediated Inhibition of PIF4 Function during Hypocotyl Growth in Arabidopsis 被引量:4
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作者 Ju-Heon Kim Hyo-Jun Lee +2 位作者 Jae-Hoon Jung Sangmin Lee Chung-Mo Park 《Molecular Plant》 SCIE CAS CSCD 2017年第2期274-284,共11页
Upon exposure to light, developing seedlings undergo photomorphogenesis, as illustrated by inhibition of hypocotyl elongation, cotyledon opening, and leaf greening. During hypocotyl photomorphogenesis, light signals a... Upon exposure to light, developing seedlings undergo photomorphogenesis, as illustrated by inhibition of hypocotyl elongation, cotyledon opening, and leaf greening. During hypocotyl photomorphogenesis, light signals are sensed by multiple photoreceptors, among which the red/far-red light-sensing phytochromes have been extensively studied. However, it is not fully understood how the phytochromes modulate hypo- cotyl growth. Here, we demonstrated that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1), which is known to either act as E3 ubiquitin ligase or affect chromatin organization, inhibits the transcriptional activation activity of PHYTOCHROME INTERACTING FACTOR 4 (PIF4), a key transcrip- tion factor that promotes hypocotyl growth. Consistent with the negative regulatory role of HOSl in hypo- cotyl growth, HOSl-defective mutants exhibited elongated hypocotyls in the light. Notably, phyB induces HOS1 activity in inhibiting PIF4 function. Taken together, these observations provide a molecular basis for the phyB-mediated suppression of hypocotyl growth in Arabidopsis. 展开更多
关键词 PHOTOMORPHOGENESIS hypocotyl growth phytochrome signaling HOS1 pif4
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BBX11 promotes red light-mediated photomorphogenic development by modulating phyB-PIF4 signaling 被引量:4
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作者 Zhaoqing Song Yueqin Heng +6 位作者 Yeting Bian Yuntao Xiao Jiujie Liu Xianhai Zhao Yan Jiang Xing Wang Deng Dongqing Xu 《aBIOTECH》 CSCD 2021年第2期117-130,共14页
phytochrome B(phyB)acts as the red light photoreceptor and negatively regulates the growth-promoting factor PHYTOCHROME INTERACTING 4(PIF4)through a direct physical interaction,which in turn changes the expression of ... phytochrome B(phyB)acts as the red light photoreceptor and negatively regulates the growth-promoting factor PHYTOCHROME INTERACTING 4(PIF4)through a direct physical interaction,which in turn changes the expression of a large number of genes.phyB-PIF4 module regulates a variety of biological and developmental processes in plants.In this study,we demonstrate that B-BOX PROTEIN 11(BBX11)physically interacts with both phyB and PIF4.BBX11 negatively regulates PIF4 accumulation as well as its biochemical activity,consequently leading to the repression of PIF4-controlled genes’expression and promotion of photomorphogenesis in the prolonged red light.This study reveals a regulatory mechanism that mediates red light signal transduction and sheds a light on phyB-PIF4 module in promoting red light-dependent photomorphognenesis. 展开更多
关键词 PHYB BBX pif4 PHOTOMORPHOGENESIS Light signaling
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COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth 被引量:2
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作者 Hongxia Lan Yueqin Heng +7 位作者 Jian Li Mengdi Zhang Yeting Bian Li Chu Yan Jiang Xuncheng Wang Dongqing Xu Xing Wang Deng 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2022年第11期2097-2110,共14页
Light signaling precisely controls photomorphogenic development in plants.PHYTOCHROME INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental process.In this study,we repor... Light signaling precisely controls photomorphogenic development in plants.PHYTOCHROME INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental process.In this study,we report CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6(CSU6)functions as a key regulator of light signaling.Loss of CSU6 function largely rescues the cop1-6 constitutively photomorphogenic phenotype.CSU6 promotes hypocotyl growth in the dark,but inhibits hypocotyl elongation in the light.CSU6 not only associates with the promoter regions of PIF4 and PIF5 to inhibit their expression in the morning,but also directly interacts with both PIF4 and PIF5 to repress their transcriptional activation activity.CSU6 negatively controls a group of PIF4-and PIF5-regulated gene expressions.Mutations in PIF4 and/or PIF5 are epistatic to the loss of CSU6,suggesting that CSU6 acts upstream of PIF4 and PIF5.Taken together,CSU6 promotes light-inhibited hypocotyl elongation by negatively regulating PIF4 and PIF5 transcription and biochemical activity. 展开更多
关键词 COP1 CSU6 light signaling PHOTOMORPHOGENESIS pif4 PIF5
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SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5-and PIF4-mediated signaling 被引量:2
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作者 Tao Li Haojie Li +8 位作者 Hongmei Lian Pengyu Song Yulong Wang Jie Duan Zhaoqing Song Yan Cao Dongqing Xu Jigang Li Huiyong Zhang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2022年第9期1706-1723,共18页
Arabidopsis CONSTITUTIVELY PHOTOMORPHO GENIC1(COP1)and PHYTOCHROME INTERACTING FACTORs(PIFs)are negative regulators,and ELONGATED HYPOCOTYL5(HY5)is a positive regulator of seedling photomorphogenic development.Here,we... Arabidopsis CONSTITUTIVELY PHOTOMORPHO GENIC1(COP1)and PHYTOCHROME INTERACTING FACTORs(PIFs)are negative regulators,and ELONGATED HYPOCOTYL5(HY5)is a positive regulator of seedling photomorphogenic development.Here,we report that SICKLE(SIC),a proline rich protein,acts as a novel negative regulator of photomorphogenesis.HY5 directly binds the SIC promoter and activates SIC expression in response to light.In turn,SIC physically interacts with HY5 and interferes with its transcriptional regulation of downstream target genes.Moreover,SIC interacts with PIF4 and promotes PIF4-activated transcription of itself.Interestingly,SIC is targeted by COP1 for 26S proteasomemediated degradation in the dark.Collectively,our data demonstrate that light-induced SIC functions as a brake to prevent exaggerated light response via mediating HY5 and PIF4 signaling,and its degradation by COP1 in the dark avoid too strong inhibition on photomorphogenesis at the beginning of light exposure. 展开更多
关键词 ARABIDOPSIS COP1-HY5 module pif4 PHOTOMORPHOGENESIS SICKLE
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WRKY2 and WRKY10 regulate the circadian expression of PIF4 during the day through interactions with CCA1/LHY and phyB 被引量:1
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作者 Shulei Wang Qingbin Sun +2 位作者 Min Zhang Chengzhu Yin Min Ni 《Plant Communications》 SCIE 2022年第2期121-137,共17页
WRKY transcription factors are known mostly for their function in plant defense,abiotic stress responses,senescence,seed germination,and development of the pollen,embryo,and seed.Here,we report the regulatory function... WRKY transcription factors are known mostly for their function in plant defense,abiotic stress responses,senescence,seed germination,and development of the pollen,embryo,and seed.Here,we report the regulatory functions of two WRKY proteins in photomorphogenesis and PIF4 expression.PIF4 is a critical signaling hub in light,temperature,and hormonal signaling pathways.Either its expression or its accumulation peaks in the morning and afternoon.WRKY2 and WRKY10 form heterodimers and recognize their target site in the PIF4 promoter near the MYB element that is bound by CCA1 and LHY under red and blue light.WRKY2 and WRKY10 interact directly with CCA1/LHY to enhance their targeting but interact indirectly with SHB1.The two WRKY proteins also interact with phyB,and their interaction enhances the targeting of CCA1 and LHY to the PIF4 promoter.SHB1 associates with theWRKY2 andWRKY10 loci and enhances their expression in parallel with the PIF4 expression peaks.This forward regulatory loop further sustains the accumulation of the two WRKY proteins and the targeting of CCA1/LHY to the PIF4 locus.In summary,interactions of two WRKY proteins with CCA1/LHY and phyB maintain an optimal expression level of PIF4 toward noon and afternoon,which is essential to sketch the circadian pattern of PIF4 expression. 展开更多
关键词 CCA1 LHY light signaling pif4 expression WRKY2 WRKY10
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PIF4 and HOOKLESS1 Impinge on Common Transcriptome and Isoform Regulation in Thermomorphogenesis 被引量:1
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作者 Huanhuan Jin Jingya Lin Ziqiang Zhu 《Plant Communications》 2020年第2期45-53,共9页
High temperature activates the transcription factor PHYTOCHROME-INTERACTING FACTOR4(PIF4)to stimulate auxin signaling,which causes hypocotyl elongation and leaf hyponasty(thermomorphogenesis).HOOKLESS1(HLS1)is a recen... High temperature activates the transcription factor PHYTOCHROME-INTERACTING FACTOR4(PIF4)to stimulate auxin signaling,which causes hypocotyl elongation and leaf hyponasty(thermomorphogenesis).HOOKLESS1(HLS1)is a recently reported positive regulator of thermomorphogenesis,but the molecular mechanisms by which HLS1 regulates thermomorphogenesis remain unknown.In this study,we initially compared PIF4-and/or HLS1-dependent differential gene expression(DEG)upon high-temperature treatment.We found that a large number of genes are coregulated by PIF4 and HLS1,especially genes involved in plant growth or defense responses.Moreover,we found that HLS1 interacts with PIF4 to form a regulatory module and that,among the HLS1-PIF4-coregulated genes,27.7%are direct targets of PIF4.We also identified 870 differentially alternatively spliced genes(DASGs)in wild-type plants under high temperature.Interestingly,more than half of these DASG events(52.4%)are dependent on both HLS1 and PIF4,and the spliceosome-defective mutant plantsexhibit a hyposensitive response to high temperature,indicating that DASGs are required for thermomorphogenesis.Further comparative analyses showed that the HLS1/PIF4-coregulated DEGs and DASGs exhibit almost no overlap,suggesting that high temperature triggers two distinct strategies to control plant responses and thermomorphogenesis.Taken together,these results demonstrate that the HLS1-PIF4 module precisely controls both transcriptional and posttranscriptional regulation during plant thermomorphogenesis. 展开更多
关键词 pif4 HLS1 TRANSCRIPTION alternative splicing thermomorphogenesis
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SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth
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作者 Junling Huai Xinyu Zhang +4 位作者 Jialong Li Tingting Ma Ping Zha Yanjun Jing Rongcheng Lin 《Molecular Plant》 SCIE CAS CSCD 2020年第12期1825-1825,共1页
(Molecular Plant 11(7):928-942;July 2018;https://doi.Org/10.1016/j.molp.2018.04.005)In the Abstract of this article,there is an error in the sentence"SEU associates with the regulatory regions of INDOLE-3-ACETIC ... (Molecular Plant 11(7):928-942;July 2018;https://doi.Org/10.1016/j.molp.2018.04.005)In the Abstract of this article,there is an error in the sentence"SEU associates with the regulatory regions of INDOLE-3-ACETIC ACID INDUCIBLE6(IAA6)and IAA19 in a PIF4-independent manner,whereas the binding of PIF4 to these genes requires SEU.Furthermore. 展开更多
关键词 pif4 ACID SENTENCE
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茶树Cs HIPP26.1互作蛋白的筛选与验证 被引量:3
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作者 范延艮 王域 +3 位作者 刘富浩 赵秀秀 向勤锃 张丽霞 《中国农业科学》 CAS CSCD 北大核心 2022年第8期1630-1641,共12页
【背景】‘黄金芽’属于光照敏感型黄化茶树(Camellia sinensis)品种,叶片色泽呈现强光黄化、弱光复绿的特点,但叶色响应光照的黄化机制并不明确。前期通过对黄化叶片、遮阴复绿叶片以及常绿品种叶片的蛋白组研究发现,重金属相关异戊二... 【背景】‘黄金芽’属于光照敏感型黄化茶树(Camellia sinensis)品种,叶片色泽呈现强光黄化、弱光复绿的特点,但叶色响应光照的黄化机制并不明确。前期通过对黄化叶片、遮阴复绿叶片以及常绿品种叶片的蛋白组研究发现,重金属相关异戊二烯化植物蛋白CsHIPP26.1(TEA000549)的表达响应光照强度,表明CsHIPP26.1可能参与调控‘黄金芽’叶色黄化的光响应过程。【目的】通过筛选与CsHIPP26.1互作的光信号响应相关的蛋白,为叶片色泽响应光照信号变化提供科学依据。【方法】以‘黄金芽’茶树1芽2叶为材料进行CsHIPP26.1和互作基因的克隆,经酵母双杂交筛库,然后将筛选得到的目的蛋白进行酵母双杂交点对点验证、体内双分子荧光互补(BiFC)和体外pull-down等技术进行蛋白互作的进一步验证。【结果】通过酵母双杂交对茶树cDNA文库进行筛选,共筛选到26个候选互作蛋白,主要集中在细胞组分、结合以及催化活性方面发挥作用,其中生物素合成代谢过程富集程度较高,与光信号通路及叶绿素合成相关的蛋白只有编号为TEA026466.1的bHLH30转录因子。克隆bHLH30转录因子后发现,该转录因子与茶树光信号传导途径蛋白PIF4处于同一进化树分支,且含有与茶树PIF4蛋白相同的HLH和ACT结构域,将该bHLH30转录因子命名为CsPIF4.2,GenBank登记号为MW116834。进一步通过体外Pull-down蛋白互作和体内双分子荧光互补(BiFC)验证发现,CsHIPP26.1和CsPIF4.2蛋白能够发生互作,并且发生互作的部位在细胞核内。【结论】初步筛选出26个与CsHIPP26.1互作的蛋白,并验证发现CsHIPP26.1能够在细胞核内与其中一个光敏色素互作因子CsPIF4.2发生蛋白相互作用。 展开更多
关键词 HIPP pif4 蛋白互作 黄化品种 光响应
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