目的探究胃癌(GC)患者血清环状RNA circ-PVT1和miR-423-5p的水平变化及其与淋巴结转移的关系。方法选取2020年6月至2022年6月湖州市第一人民医院收治的GC患者100例,根据治疗后淋巴结转移情况分为转移组(64例)和未转移组(36例)。取同期...目的探究胃癌(GC)患者血清环状RNA circ-PVT1和miR-423-5p的水平变化及其与淋巴结转移的关系。方法选取2020年6月至2022年6月湖州市第一人民医院收治的GC患者100例,根据治疗后淋巴结转移情况分为转移组(64例)和未转移组(36例)。取同期胃炎、胃溃疡、胃息肉等患者100例作为良性病变组,同期体检健康者100例作为健康人对照组。采用qRT-PCR检测各组血清circ-PVT1、miR-423-5p水平,分析血清circ-PVT1、miR-423-5p水平与GC患者治疗后淋巴结转移的关系;Logistic回归分析GC患者治疗后淋巴结转移的影响因素;ROC曲线分析血清circ-PVT1、miR-423-5p水平对GC患者治疗后淋巴结转移的预测价值。结果与健康人对照组相比,良性病变组和GC组患者血清circ-PVT1水平(1.04±0.30 vs 1.47±0.39 vs 1.83±0.54)显著上调,miR-423-5p水平(1.02±0.28 vs 0.85±0.20 vs 0.51±0.14)显著下降(F分别为87.933、146.594,P<0.05);与良性病变组相比,GC组患者血清circ-PVT1水平显著上调,miR-423-5p水平显著下降。血清circ-PVT1、miR-423-5p水平单独及二者联合诊断GC的ROC曲线下面积(AUC^(ROC))分别为0.729、0.817、0.869,其中二者联合检测的AUC^(ROC)显著高于单独检测(Z分别为3.211、2.133,P<0.05)。与淋巴结未转移组相比,淋巴结转移组GC患者血清circ-PVT1水平(1.66±0.51 vs 1.92±0.58)显著上调,miR-423-5p水平(0.74±0.22 vs 0.38±0.16)显著下降(P<0.05)。Logistic回归分析结果显示,肿瘤直径、分化程度、TNM分期、高水平circ-PVT1是GC患者治疗后淋巴结转移的独立危险因素(P<0.05),高水平miR-423-5p是GC患者治疗后淋巴结转移的独立保护因素(P<0.05)。ROC曲线结果显示,血清circ-PVT1、miR-423-5p水平单独及二者联合预测GC患者淋巴结转移的AUC^(ROC)分别为0.707、0.753、0.841,二者联合预测AUC^(ROC)显著高于单独预测(Z分别为3.595、5.167,P<0.05)。circ-PVT1和miR-423-5p存在靶向关系,且GC患者血清circ-PVT1水平与miR-423-5p水平呈显著负相关(r=-0.546,P<0.05)。结论GC患者血清circ-PVT1水平升高,miR-423-5p水平降低,且均与GC治疗后淋巴结转移密切相关,二者联合对GC治疗后淋巴结转移有较高的预测价值。展开更多
Research has shown that long-chain noncoding RNAs(lncRNAs) are involved in the regulation of a variety of biological processes, including peripheral nerve regeneration, in part by acting as competing endogenous RNAs. ...Research has shown that long-chain noncoding RNAs(lncRNAs) are involved in the regulation of a variety of biological processes, including peripheral nerve regeneration, in part by acting as competing endogenous RNAs. c-Jun plays a key role in the repair of peripheral nerve injury. However, the precise underlying mechanism of c-Jun remains unclear. In this study, we performed microarray and bioinformatics analysis of mouse crush-injured sciatic nerves and found that the lncRNA Pvt1 was overexpressed in Schwann cells after peripheral nerve injury. Mechanistic studies revealed that Pvt1 increased c-Jun expression through sponging miRNA-214. We overexpressed Pvt1 in Schwann cells cultured in vitro and found that the proliferation and migration of Schwann cells were enhanced, and overexpression of miRNA-214 counteracted the effects of Pvt1 overexpression on Schwann cell proliferation and migration. We conducted in vivo analyses and injected Schwann cells overexpressing Pvt1 into injured sciatic nerves of mice. Schwann cells overexpressing Pvt1 enhanced the regeneration of injured sciatic nerves following peripheral nerve injury and the locomotor function of mice was improved. Our findings reveal the role of lncRNAs in the repair of peripheral nerve injury and highlight lncRNA Pvt1 as a novel potential treatment target for peripheral nerve injury.展开更多
文摘目的探究胃癌(GC)患者血清环状RNA circ-PVT1和miR-423-5p的水平变化及其与淋巴结转移的关系。方法选取2020年6月至2022年6月湖州市第一人民医院收治的GC患者100例,根据治疗后淋巴结转移情况分为转移组(64例)和未转移组(36例)。取同期胃炎、胃溃疡、胃息肉等患者100例作为良性病变组,同期体检健康者100例作为健康人对照组。采用qRT-PCR检测各组血清circ-PVT1、miR-423-5p水平,分析血清circ-PVT1、miR-423-5p水平与GC患者治疗后淋巴结转移的关系;Logistic回归分析GC患者治疗后淋巴结转移的影响因素;ROC曲线分析血清circ-PVT1、miR-423-5p水平对GC患者治疗后淋巴结转移的预测价值。结果与健康人对照组相比,良性病变组和GC组患者血清circ-PVT1水平(1.04±0.30 vs 1.47±0.39 vs 1.83±0.54)显著上调,miR-423-5p水平(1.02±0.28 vs 0.85±0.20 vs 0.51±0.14)显著下降(F分别为87.933、146.594,P<0.05);与良性病变组相比,GC组患者血清circ-PVT1水平显著上调,miR-423-5p水平显著下降。血清circ-PVT1、miR-423-5p水平单独及二者联合诊断GC的ROC曲线下面积(AUC^(ROC))分别为0.729、0.817、0.869,其中二者联合检测的AUC^(ROC)显著高于单独检测(Z分别为3.211、2.133,P<0.05)。与淋巴结未转移组相比,淋巴结转移组GC患者血清circ-PVT1水平(1.66±0.51 vs 1.92±0.58)显著上调,miR-423-5p水平(0.74±0.22 vs 0.38±0.16)显著下降(P<0.05)。Logistic回归分析结果显示,肿瘤直径、分化程度、TNM分期、高水平circ-PVT1是GC患者治疗后淋巴结转移的独立危险因素(P<0.05),高水平miR-423-5p是GC患者治疗后淋巴结转移的独立保护因素(P<0.05)。ROC曲线结果显示,血清circ-PVT1、miR-423-5p水平单独及二者联合预测GC患者淋巴结转移的AUC^(ROC)分别为0.707、0.753、0.841,二者联合预测AUC^(ROC)显著高于单独预测(Z分别为3.595、5.167,P<0.05)。circ-PVT1和miR-423-5p存在靶向关系,且GC患者血清circ-PVT1水平与miR-423-5p水平呈显著负相关(r=-0.546,P<0.05)。结论GC患者血清circ-PVT1水平升高,miR-423-5p水平降低,且均与GC治疗后淋巴结转移密切相关,二者联合对GC治疗后淋巴结转移有较高的预测价值。
基金supported by the National Natural Science Foundation of China,No. 81801213 (to BP)Xuzhou Special Fund for Promoting Scientific and Technological Innovation,Nos. KC21177 (to BP),KC21195 (to HF)Science and Technology Project of Yili Kazak Autonomous Prefecture,No. YZ2019D006 (to HF)。
文摘Research has shown that long-chain noncoding RNAs(lncRNAs) are involved in the regulation of a variety of biological processes, including peripheral nerve regeneration, in part by acting as competing endogenous RNAs. c-Jun plays a key role in the repair of peripheral nerve injury. However, the precise underlying mechanism of c-Jun remains unclear. In this study, we performed microarray and bioinformatics analysis of mouse crush-injured sciatic nerves and found that the lncRNA Pvt1 was overexpressed in Schwann cells after peripheral nerve injury. Mechanistic studies revealed that Pvt1 increased c-Jun expression through sponging miRNA-214. We overexpressed Pvt1 in Schwann cells cultured in vitro and found that the proliferation and migration of Schwann cells were enhanced, and overexpression of miRNA-214 counteracted the effects of Pvt1 overexpression on Schwann cell proliferation and migration. We conducted in vivo analyses and injected Schwann cells overexpressing Pvt1 into injured sciatic nerves of mice. Schwann cells overexpressing Pvt1 enhanced the regeneration of injured sciatic nerves following peripheral nerve injury and the locomotor function of mice was improved. Our findings reveal the role of lncRNAs in the repair of peripheral nerve injury and highlight lncRNA Pvt1 as a novel potential treatment target for peripheral nerve injury.
