Resistance index and browning mechanism of apple peel under high temperature stress

Apples are one of the most important economic crops worldwide.Because of global warming and an aggravation of environmental,abnormally high temperatures occur frequently in fruit-growing season and seriously affect normal fruit growth and reduce fruit quality and yield.We took five-year-old Ruixue’(Qinfu 1×Pink Lady;CNA20151469.1) fruits as test materials,and the ambient temperature during fruit development was monitored.The results showed that during the fruit-growing season,especially during the rapid growth stage (July to August),the maximum daily temperature exceeded 30℃ and lasted for more than 40 days.To determine the effects of high temperature stress on the apple fruit resistance,we treated expanding,veraison,and maturity-period fruits at different temperatures.It was found that the fruits of the expanding period showed strong resistance to high temperature stress,whereas during veraison and maturity,fruit resistance to high temperature stress decreased,and the fruit peel browning phenotype appeared.Meanwhile,the content of malonaldehyde (MDA),hydrogen peroxide (H_(2)O_(2)),and superoxide anion (O._(2)^(-)) in the peel gradually increased with increasing temperature.The content of total phenols,flavanol,and flavonoids in the peel decreased substantially at 45℃.Moreover,it was found that polyphenol oxidase gene (MdPPO1) was most sensitive to high temperature stress in apple.Furthermore,transient and stable MdPPO1 overexpression significantly promoted peel browning.The transgenic materials were more sensitive to high temperatures,and browning was more severe compared to non-genetically modified organism (WT).Stable MdPPO1 knockout calli obtained via clustered regularly interspersed short palindromic repeats (CRISPR/Cas9) gene knockout technology reduced the browning phenotype,and the resultant fruits were not sensitive to the effects of high temperature stress.Thus,MdPPO1 expression may be a key factor of high temperature-related changes observed in the browning phenotype that provides a scientific theoretical basis for the selection of high temperature-resistant varieties and apple cultivation and management in the future.

Effects of vitrectomy combined with internal limiting membrane peeling in patients with diabetic macular edema

BACKGROUND Diabetic macular edema(DME),a chronic microvascular complication of diabetes,is a leading cause of visual impairment and blindness.Pars plana vitrectomy(PPV)can restore the normal macular structure and reduce macular edema,whereas internal limiting membrane(ILM)peeling is used to treat tractional macular diseases.Despite the advantages,there is limited research on the combined effects of PPV with ILM peeling.AIM To observe the effects of PPV combined with ILM peeling on postoperative central macular thickness(CMT),best-corrected visual acuity(BCVA),cystoid macular edema(CME)volume,and complications in patients with DME.METHODS Eighty-one patients(92 eyes)diagnosed with DME at the Beijing Shanqu Liangxiang Hospital between January and December 2022 were randomly divided to undergo PPV alone(control group:41 patients,47 eyes)or PPV+ILM peeling(stripping group:40 patients,45 eyes);a single surgeon performed all surgeries.The two groups were compared preoperatively and 1 and 3 months postoperatively.RESULTS Preoperatively,both groups had comparable values of CMT,BCVA,and CME volume(P>0.05).After surgery(both 1 and 3 months),both groups showed significant reductions in CMT,BCVA,and CME volume compared to preoperative levels,with the stripping group showing more significant reductions compared to the control group(P<0.05).Further repeated-measures ANOVA analysis for within-group differences revealed significant effects of group and time,and interaction effects for CMT,BCVA,and CME volume(P<0.05).There were no significant differences in the incidence of complications between the groups(retinal detachment:control=2,stripping=1;endophthalmitis:Control=4,stripping=1;no cases of secondary glaucoma or macular holes;χ^(2)=0.296,P=0.587).CONCLUSION PPV with ILM peeling can significantly improve the visual acuity of patients with DME,reduce CMT,and improve CME with fewer complications.

Green Synthesis of Silver Nanoparticles Using Aqueous Orange and Lemon Peel Extract and Evaluation of Their Antimicrobial Properties

Green synthesis of silver nanoparticles (AgNPs) using aqueous extracts of orange and lemon peels, as a reducing agent, and silver nitrate salts as a source of silver ions is a promising field of research due to the versatility of biomedical applications of metal nanoparticles. In this paper, AgNPs were synthetized at different reaction parameters such as the type and concentration of the extracts, metal salt concentration, temperature, speed stirring, and pH. The antibacterial properties of the obtained silver nanoparticles against E. coli, as well as the physical and chemical characteristics of the synthesized silver nanoparticles, were investigated. UV-Vis spectroscopy was used to confirm the formation of AgNPs. In addition to green biogenic synthesis, chemical synthesis of silver nanoparticles was also carried out. The optimal temperature for extraction was 65˚C, while for the synthesis of AgNPs was 35˚C. The synthesis is carried out in an acidic environment (pH = 4.7 orange and pH = 3.8 lemon), neutral (pH = 7) and alkaline (pH = 10), then for different concentrations of silver nitrate solution (0.5 mM - 1 mM), optimal time duration of the reaction was 60 min and optimal stirring speed rotation was 250 rpm on the magnetic stirrer. The physical properties of the synthesized silver nanoparticles (conductivity, density and refractive index) were also studied, and the passage of laser light through the obtained solution and distilled water was compared. Positive inhibitory effect on the growth of new Escherichia coli colonies have shown AgNPs synthesized at a basic pH value and at a 0.1 mM AgNO3 using orange or lemon peel extract, while for a 0.5 mM AgNO3 using lemon peel extract.

Optimization of the Pretreatment of the Mixture of Cassava Peelings and Pineapple Fibers Using Response Surface Methodology and a Process Simulator for the Bioethanol Production

The increase in oil prices and greenhouse gas emissions has led to the search for substitutes for fossil fuels. In Cameroon, the abundance of lignocellulosic resources is inherent to agricultural activity. Production of bioethanol remains a challenge given the crystallinity of cellulose and the presence of the complex. The pretreatment aimed to solubilize the lignin fraction and to make cellulose more accessible to the hydrolytic enzymes, was done using the organosolv process. A mathematical modeling was performed to point out the effect of the temperature on the kinetics of the release of the reducing sugars during the pretreatment. Two mathematical model was used, SAEMAN’s model and Response surface methodology. The first show that the kinetic parameters of the hydrolysis of the cellulose and reducing sugar are: 0.05089 min-1, 5358.1461 J·mol-1, 1383.03691 min-1, 51577.6100 J·mol-1 respectively. The second model was used. Temperature is the factor having the most positive influence whereas, ethanol concentration is not an essential factor. To release the maximum, an organosolv pre-treatment of this sub-strate should be carried out at 209.08°C for 47.60 min with an ethanol-water ratio of 24.02%. Organosolv pre-treatment is an effective process for delignification of the lignocellulosic structure.

Extraction, Production and Quality Evaluation of Margarine from Oil Extracted from Waste Biomass Peels of Avocado and Virgin Coconut Oil, Using Chitosan from Reared Shells as Preservative

The production and consumption of avocado pears generates tons of wastes, mainly the pear peels which are usually discarded, although they have been reported to contain important phyto-chemicals with biological activities. The adverse health effect associated with the consumption of saturated lipid based foods has ignited research on reformulation of lipid based foods to eliminate Trans Fatty Acids (TFAs). This study was thus aimed at the extraction and characterization of oil from Avocado Peels (APO) and evaluation of the quality of margarine produced from it. Five verities of pear were used for oil extraction by soxhlet method and physiochemical, oxidative, functional and antioxidant characterization was done. Margarines were formulated using a central composite design using oil blends of APO and Virgin Coconut Oil (VCO) with an oil ratio of 10:90, 40:60, 70:30 respectively, varied blending speed, blending time, and chitosan concentration. Samples were characterized and the effect of process parameters on the physiochemical and functional properties of the margarine studied. Optimized conditions were used to produce samples for sensory evaluation. Color, spreadability, aroma, taste and general acceptability was evaluated using ranking difference test. The results showed that the yield, density, and iodine values of APOs oils ranged from 14.91 ± 0.18 to 11.76 ± 0.46;0.93 ± 0.001 to 0.99 ± 0.1;46.63 ± 1.70 to 52.4 ± 0.63, their acid values, TBA and PV values ranged from 1.42 ± 0.39 to 1.97 ± 0.5;0.11 ± 0.002 to 0.18 ± 0.04;and 2.72 ± 0.14 to 4.43 ± 0.36 respectively, with Brogdon avocado peel variety having the overall best properties prepared blends of trans-free APO margarines showed that increase in APO ratio decreased melting point, increased oxidative stability and reduced moisture content of margarine samples. Chitosan addition leads to decrease moisture content and increase functional properties. VCO lead to increase in phenolic and flavonoid content of the margarines. Samples were spreadable and palatable with R20 being most palatable and the most accepted being R26 with a mean score of 7.07 ± 0.70. Decrease in color intensity increased acceptability. This study therefore demonstrated that avocado peel waste biomass can be valorized by using it as raw material for oil extraction, which can serve as good material for the production of trans-free margarines with good oxidative stability, functional and antioxidant properties.

Characterization of adsorptive capacity and mechanisms on adsorption of copper, lead and zinc by modified orange peel

A novel adsorbent was prepared by modifying orange peel with sodium hydroxide and calcium chloride. The morphological and characteristics of the adsorbent were evaluated by infrared spectroscopy （IR）, scanning electron microscopy （SEM） and N2-adsorption techniques. The adsorption behavior of Cu^2＋, Pb^2＋ and Zn^2＋ on modified orange peel （SCOP） was studied by varying parameters like pH, initial concentration of metal ions. Equilibrium was well described by Langmuir equation with the maximum adsorption capacities for Cu^2＋, Pb^2＋ and Zn^2＋ of 70.73, 209.8 and 56.18 mg/g, respectively. Based on the results obtained in batch experiments, breakthrough profiles were examined using a column packed with SCOP for the separation of small concentration of Pb^2＋ from an excess of Zn^2＋ followed by elution tests. Ion exchange with Ca^2＋ neutralizing the carboxyl groups of the pectin was found to be the predominant mechanism.

Study on Antioxidation Effect of Polyphenols from Pomegranate Peel in Vivo

[Objective] The aim was to study the antioxidant effect of polyphenols from pomegranate peel in vivo. [Method] The Kunming rats were randomly divided into a control group, a low-dose group, a middle-dose group and a high-dose group,n=10; the protein content, the activities of the superoxide dismutase（SOD） and the glutathione peroxidase（GSH-PX）, the content of the maleic dialdehyde（MDA） in serum and liver tissue of the rats from different groups were determined. [Result]The polyphenols of pomegranate peel could increase protein content, activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） in serum and liver tissue, and decrease the maleic dialdehyde（MDA） content simultaneously. [Conclusion] Polyphenols of pomegranate peel have strong antioxidant activity in vivo.

Optimization of Chromatographic Conditions for Detecting Ellagic Acid in Pomegranate Peels Using HPLC Method

[Objective] This study aimed to optimize the chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method. [Method] By using 0.2 mg/ml ellagic acid standard solution, on the basis of single-factor experiment and orthogonal experiment, chromatographic conditions （mobile phase ratio, flow rate, col- umn temperature） for detecting ellagic acid using HPLC were optimized. Based on the optimal chromatographic conditions, the ellagic acid content in experimental pomegranate peels was determined. [Resull] The optimal chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method are： 1.2% phos- phoric acid：acetonitrile=85：15, column temperature of 35 ℃, and flow rate of 1.0 ml/min. The linear regression equation of ellagic acid is： y=2.9e＋0.6x＋4.4e＋5 （FF=9 999）. Ac- cording to the standard addition recovery test, the average recovery rate of ellagic acid is 98.20%, and RSD is 0.60%. Under above optimized chromatographic condi- tions, ellagic acid can be well separated from other interfering components in pomegranate peels, with shorter peak time and ideal effect, which is convenient for the detection in production practices. [Conclusion] This study laid the foundation for detecting ellagic acid in pomegranate peels using HPLC method.

Peel-away塑料薄膜鞘结合输尿管镜气压弹道碎石术治疗女性输尿管中下段复杂性结石的临床应用价值

目的:探讨Peel-away塑料薄膜鞘结合输尿管镜气压弹道碎石术治疗女性输尿管中下段复杂性结石的临床应用价值。方法:选取2014年5月-2018年5月期间收治的输尿管中下段复杂性结石患者84例,均为女性,根据随机数表法随机分为观察组和对照组,每组各42例,其中对照组患者给予输尿管镜气压弹道碎石术治疗,观察组患者采用Peel-away塑料薄膜鞘结合输尿管镜气压弹道碎石术治疗,比较两组患者碎石成功率,观察两组患者手术相关指标(手术取石时间、出血量、术后住院时间、住院费用),对两组患者肾功能相关指标(SCr、BUN、IgG)进行比较,并观察两组患者并发症发生情况。结果:经过比较,观察组患者碎石成功率和碎石清除率均高于对照组(P<0.05)。观察组患者取石时间、出血量、住院时间、住院费用均明显少于对照组(P<0.05)。治疗前,两组患者SCr、BUN、IgG差异无统计学意义(P>0.05);治疗后,两组肾功能指标均降低,且观察组SCr、BUN、IgG降低程度更加明显,差异有统计学意义(P<0.05)。两组并发症发生率差异有统计学意义(P<0.05)。结论:Peel-away塑料薄膜鞘建立桥鞘通道可改善女性输尿管中下段复杂性结石患者肾功能指标,降低术中出血量、住院时间等指标,且并发症较少,值得临床进一步推广。

PEEL预后指数评估急性心肌梗死危险程度的护理意义

对307例AMI病人采用PEEL预后指数评分方法进行评分,并进行危险程度分级。结果 AMI的病人PEEL预后指数越大,危险程度越高,住院早期病死率越高。随着年龄的增加,既往史、休克、心力衰竭、ECG异常和心律失常程度的加重,AMI病人住院早期病死率亦随之增加,差异有显著性意义(均P<0.01)。提示AMI病人PEEL预后指数与住院早期病死率密切相关,是判断AMI预后简便而可靠的方法。

Peeling与韦登斯基现象

在房室超常传导受到普遍怀疑之后,韦登斯基现象往往被认为是真正的房室超常传导。这一认识是否符合实验和临床电生理研究的结果?本文作一客观的讨论。韦登斯基现象回顾 1986年韦登斯基在神经—肌肉标本上发现,对神经作阈下刺激,肌肉无反应。然而对神经作强刺激引起肌肉收缩后,原来的阈下刺激此时也能引起肌肉收缩,即所谓韦登斯基效应(Wedensky effect)。

Peel预后指数及Norris预后指数对估计急性心肌梗塞病人预后的价值

用 Peel 预后指数及 Norris 预后指数对我院住院急性心肌梗塞80例进行评分,探讨这两套预后指数在 AMI 病人近期预后估计方面的意义。结果提示:Peel 预后指数及 Norris 预后指数平均28天实际死亡率与标准死亡率呈良好相关性(γ分别为0.956和0.977)。因此,我们认为:Peel 预后指数和 Norris 预后指数选用项目容易取得,不需要复杂检查和昂贵的设备、结果可靠,具有实际应用价值,可以作为一般医院住院的 AMI 病人的预后判断的临床依据。

Preparation on Nanoemulsion of Pomegranate Peel Polyphenol

[Objective] The aim was to obtain the preparation technology of the pomegranate peel polyphenol nanoemulsion. [Method] The pomegranate peel polyphe- nol nanoemulsion was prepared by the titration methods and the pseudo ternary dia- gram. The effect of various elements on the formation of the nanoemulsion was stud- ied. [Results] The optimal prescription of the pomegranate peel polyphenol nanoemul- sion was 4.4%（w/w） of pomegranate peel polyphenol, 34.1%（w/w） of EL-40, 17.1%（w/w） of anhydrous ethanol, 5.7%（w/w） of IPM and 38.7%（w/w） of distilled water. [Conclusion] It is feasible to produce the pomegranate peel polyphenol nanoemulsion by the titration methods and the pseudo ternary diagram. It consists of pomegranate peel polyphe- nol, EL-40, anhydrous ethanol, IPM and distilled water. There is 4.4%（w/w） of the pomegranate peel polyphenol in the nanoemuJsion.

A Comparative Study on Chemical Composition of Supercritical CO_2 Extraction Products in Peels of Trichosanthes kirilowii Maxim. from Changqing District

[Objective] This study aimed to investigate the differences in chemical composition of supercritical CO2 extraction products in peels of Trichosanthes kirilowii Maxim. from Changqing district. [Method] Supercritical fluidextraction （SFE） and GCMS method were applied to determine and analyze the chemical components of the extracts in peels of three strains of Trichosanthes kirilowii Maxim. [Result] The chemical components of supercritical CO2 extraction products in peels of three strains of Trichosanthes kirilowii Maxim. varied., and the number of chemical components with normalized percentage content higher than 1% was 5, 7 and 8, respectively. There are 14 kinds of common components, and the relative content of hexadecanoic acid was the highest. [Conclusion] Supercritical CO2 extracts in peels of different strains of Trichosanthes kirilowii Maxim. contain different chemical components, providing scientific basis for breeding excellent varieties and the development and utilization of Trichosanthes kirilowii Maxim.

Research on Extraction of Anti-allergic Active Ingredient from Citrus Peels and Its Anti-allergic Activity

[Objective] This study aimed to determine the optimal process conditions for extraction of anti-allergic active ingredient from citrus peels. [Method] Crude anti- allergic active extracts of citrus peels were extracted. Effects of extraction conditions including extraction temperature, ethanol concentration, extraction time and liquid-to-solid ratio on extraction were explored with single-factor experiment, and the extrac- tion process was optimized with orthogonal experiment. [Result] Based on the single- factor experiment and orthogonal experiment, the optimal extraction condition for crude anti-allergic active extracts of citrus peels were determined to be under extraction temperature of 60℃, ethanol concentration of 70%, extraction time of 60 min, liquid-to-solid ratio of 1：25 and twice extraction. [Concluslon] This process condition is of simple operation and high extraction efficiency, which is environment-friendly and suitable for the extraction of crude anti-allergic active extracts from citrus peels. This study provided a new approach and research ideas for the effective and rapid extraction of anti-allergic active ingredient in citrus peels.

经peel-away鞘输尿管镜下铥激光治疗小儿膀胱尿路上皮癌1例并文献复习

目的报告经peel-away鞘输尿管镜下铥激光治疗小儿膀胱尿路上皮癌1例,并结合文献探讨儿童膀胱尿路上皮癌的诊治要点。方法回顾性分析赣南医学院第一附属医院泌尿外科2019年8月收治的1例膀胱尿路上皮癌患儿的临床资料。患儿确诊为膀胱肿瘤后,于全麻下行经peel-away鞘输尿管镜下铥激光膀胱肿瘤整块切除术,并随访其复发情况。结果患儿手术过程顺利,手术时间20 min,术中估计出血量约5 ml。术后病理诊断:膀胱低级别非肌层浸润性乳头状尿路上皮癌。术后定期膀胱灌注化疗,随访16个月,肿瘤无复发,尿道无狭窄。结论经peel-away鞘输尿管镜下铥激光治疗小儿膀胱低级别非肌层浸润性尿路上皮癌安全可行。

Determination of Hesperidin in Citrus Peel by HPLC

[Objective] This study aimed to measure the hesperidin content in citrus peel by high performance liquid chromatography, to provide a scientific basis for quality control and identification. [Method] The hesperidin was extracted with alkaline solution at 70 ~C and pH 6-7, and the purity of hesperidin was determined by HPLC. [Result] The formula for the regression line was Y=466,097Xq3.415 0 （r=0.999 6）, identify- ing the relationship between hesperidin concentration and peak area, and the linear range was 0.2-1.4μg. The hesperidin solution was stable within 24 h at room temperature. The average recovery rate of hesperidin was 98.41%. [Conclusion] The HPLC method is rapid, simple, and with good linear relationship, can be used for routine analysis of hesperidin.

Adsorption Isotherm Study of Activated Fruit Peels for Copper Removal

Heavy metal polluted water threatens the integrity of ecosystem.The use of agricultural waste as adsorbent becomes popular due to its biodegradability and availability.However,limited research works has been done to process agricultural waste for heavy metal adsorption purpose.The objective of this study is to propose a new approach to treat banana peel using ZnCl_(2) prior to alkali and acid treatment and explore the potential of dragon fruit peels as the adsorbent for Cu^(2+)adsorption.Seven adsorption isotherm was adopted to identify the adsorption mechanism and four statistical parameters were calculated for model verification purpose.The experiment was conducted by dispersing ZnCl_(2) treated and untreated adsorbents into Cu^(2+) solution.Results showed that ZnCl_(2) treated adsorbents performed better in terms of Cu^(2+) removal compared with untreated adsorbents due to higher surface area for Cu^(2+) adsorption.The treated banana peel(Act.-B)performed approximately 30%better than the treated dragon fruit peel(Act.-D)due to the presence of hydroxyl and carboxyl groups.The equilibrium adsorptive capacity of Act.-B was 1872.8 mg Cu^(2+)/g and the experimental data fitted Freundlich isotherm well with R^(2)=0.9978,0.0161 of residual sum of error(SSE),0.0068 of χ^(2),and 0.0567 of root mean square error(RMSE)values.This suggests non⁃linear adsorption and multilayer adsorption on heterogenous surfaces.Thus,it is recommended to further improve the surface properties of Act.-B by steam pyrolysis and surface modification.