Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyz...Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly tools.Researchers are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis tasks.But,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis task.Therefore,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own needs.In this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis methods.DecodeSTORM includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and visualization.Importantly,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of methods.We believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.展开更多
综合考虑电池和SCR催化器在低温环境下的工作特性,针对低温下温度对Plug-in柴电混合动力汽车性能的影响,提出最短时间控制和燃油消耗最少问题。以SCR起燃温度和电池正常工作温度时间最短为优化目标,以电池温度、电池荷电状态(State of C...综合考虑电池和SCR催化器在低温环境下的工作特性,针对低温下温度对Plug-in柴电混合动力汽车性能的影响,提出最短时间控制和燃油消耗最少问题。以SCR起燃温度和电池正常工作温度时间最短为优化目标,以电池温度、电池荷电状态(State of Charge,SOC)和SCR催化器温度为状态变量,利用极小值原理求得最优控制策略。通过仿真对比规则控制策略,分析了在不同低温条件下基于庞特里亚金极小值原理(Pontryagin’s Minimum Principle,PMP)的最短时间和最少油耗与排放优化控制策略对整车油耗和排放的影响。展开更多
Due to soaring fuel prices and environmental concerns, hybrid electric vehicle(HEV) technology attracts more attentions in last decade. Energy management system, configuration of HEV and traffic conditions are the mai...Due to soaring fuel prices and environmental concerns, hybrid electric vehicle(HEV) technology attracts more attentions in last decade. Energy management system, configuration of HEV and traffic conditions are the main factors which affect HEV's fuel consumption, emission and performance. Therefore, optimal management of the energy components is a key element for the success of a HEV. An optimal energy management system is developed for HEV based on genetic algorithm. Then, different powertrain system component combinations effects are investigated in various driving cycles. HEV simulation results are compared for default rule-based, fuzzy and GA-fuzzy controllers by using ADVISOR. The results indicate the effectiveness of proposed optimal controller over real world driving cycles. Also, an optimal powertrain configuration to improve fuel consumption and emission efficiency is proposed for each driving condition. Finally, the effects of batteries in initial state of charge and hybridization factor are investigated on HEV performance to evaluate fuel consumption and emissions. Fuel consumption average reduction of about 14% is obtained for optimal configuration data in contrast to default configuration. Also results indicate that proposed controller has reduced emission of about 10% in various traffic conditions.展开更多
The paper proposes an adoption of slope,elevation,speed and route distance preview to achieve optimal energymanagement of plug-in hybrid electric vehicles(PHEVs).Theapproach is to identify route features from historic...The paper proposes an adoption of slope,elevation,speed and route distance preview to achieve optimal energymanagement of plug-in hybrid electric vehicles(PHEVs).Theapproach is to identify route features from historical and real-time traffic data,in which information fusion model and trafficprediction model are used to improve the information accuracy.Then,dynamic programming combined with equivalent con-sumption minimization strategy is used to compute an optimalsolution for real-time energy management.The solution is thereference for PHEV energy management control along the route.To improve the system's ability of handling changing situation,the study further explores predictive control model in the real-time control of the energy.A simulation is performed to modelPHEV under above energy control strategy with route preview.The results show that the average fuel consumption of PHEValong the previewed route with model predictive control(MPC)strategy can be reduced compared with optimal strategy andbase control strategy.展开更多
In order to achieve the improvement of the driving comfort and energy efficiency,an new e-CVT flexible full hybrid electric system(E2FHS) is proposed,which uses an integrated main drive motor and generator to take the...In order to achieve the improvement of the driving comfort and energy efficiency,an new e-CVT flexible full hybrid electric system(E2FHS) is proposed,which uses an integrated main drive motor and generator to take the place of the original automatic or manual transmission to realize the functions of continuously variable transmission(e-CVT).The design and prototype realization of the E2FHS system for a plug-in hybrid vehicle(PHEV) is performed.In order to analyze and optimize the parameters and the power flux between different parts of the E2FHS,simulation software is developed.Especially,in order to optimize the performance of the energy economy improvement of the E2FHS,the effect of the different energy management controllers is investigated,and an adaptive online-optimal energy management controller for the E2FHS is built and validated by the prototype PHEV.展开更多
The construction and characteristics of a microscale long-optical-path electrochemi- cal cell with a plug-in thin-layer electrode are described.Using ferricyanide as the test species,the thermodynamic parameters of el...The construction and characteristics of a microscale long-optical-path electrochemi- cal cell with a plug-in thin-layer electrode are described.Using ferricyanide as the test species,the thermodynamic parameters of electron transfer processes are determined at car- bon,plantinum,and gold electrodes.展开更多
A variable parameter self-adaptive control strategy based on driving condition identification is proposed to take full advantage of the fuel saving potential of the plug-in hybrid electric bus(PHEB).Firstly,the princi...A variable parameter self-adaptive control strategy based on driving condition identification is proposed to take full advantage of the fuel saving potential of the plug-in hybrid electric bus(PHEB).Firstly,the principal component analysis(PCA)and the fuzzy c-means clustering(FCM)algorithm is used to construct the comprehensive driving cycle,congestion driving cycle,urban driving cycle and suburban driving cycle of Chinese urban buses.Secondly,an improved particle swarm optimization(IPSO)algorithm is proposed,and is used to optimize the control parameters of PHEB under different driving cycles,respectively.Then,the variable parameter self-adaptive control strategy based on driving condition identification is given.Finally,for an actual running vehicle,the driving condition is identified by relevance vector machine(RVM),and the corresponding control parameters are selected to control the vehicle.The simulation results show that the fuel consumption of using the variable parameter self-adaptive control strategy is reduced by 4.2% compared with that of the fixed parameter control strategy,and the feasibility of the variable parameter self-adaptive control strategy is verified.展开更多
In this paper, a plug-in hybrid electrical vehicle(PHEV) is taken as the research object, and its dynamic performance and economic performance are taken as the research goals. Battery charge-sustaining(CS) period is d...In this paper, a plug-in hybrid electrical vehicle(PHEV) is taken as the research object, and its dynamic performance and economic performance are taken as the research goals. Battery charge-sustaining(CS) period is divided into power mode and economy mode. Energy management strategy designing methods of power mode and economy mode are proposed. Maximum velocity, acceleration performance and fuel consumption are simulated during the CS period in the AVL CRUISE simulation environment. The simulation results indicate that the maximum velocity and acceleration time of the power mode are better than those in the economy mode. Fuel consumption of the economy mode is better than that in the power mode. Fuel consumption of PHEV during the CS period is further improved by using the methods proposed in this paper, and this is meaningful for research and development of PHEV.展开更多
基金supported by the National Natural Science Foundation of China(82160345)Key research and development project of Hainan province(ZDYF2021GXJS017)+2 种基金Key Science and Technology Plan Project of Haikou(2021-016)the Start-up Fund from Hainan University(KYQD(ZR)-20022 and KYQD(ZR)-20077)the Student Innovation and Entrepreneurship Project of Biomedical Engineer-ing School,Hainan University(BMECF2D2021001).
文摘Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly tools.Researchers are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis tasks.But,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis task.Therefore,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own needs.In this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis methods.DecodeSTORM includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and visualization.Importantly,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of methods.We believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.
文摘综合考虑电池和SCR催化器在低温环境下的工作特性,针对低温下温度对Plug-in柴电混合动力汽车性能的影响,提出最短时间控制和燃油消耗最少问题。以SCR起燃温度和电池正常工作温度时间最短为优化目标,以电池温度、电池荷电状态(State of Charge,SOC)和SCR催化器温度为状态变量,利用极小值原理求得最优控制策略。通过仿真对比规则控制策略,分析了在不同低温条件下基于庞特里亚金极小值原理(Pontryagin’s Minimum Principle,PMP)的最短时间和最少油耗与排放优化控制策略对整车油耗和排放的影响。
文摘Due to soaring fuel prices and environmental concerns, hybrid electric vehicle(HEV) technology attracts more attentions in last decade. Energy management system, configuration of HEV and traffic conditions are the main factors which affect HEV's fuel consumption, emission and performance. Therefore, optimal management of the energy components is a key element for the success of a HEV. An optimal energy management system is developed for HEV based on genetic algorithm. Then, different powertrain system component combinations effects are investigated in various driving cycles. HEV simulation results are compared for default rule-based, fuzzy and GA-fuzzy controllers by using ADVISOR. The results indicate the effectiveness of proposed optimal controller over real world driving cycles. Also, an optimal powertrain configuration to improve fuel consumption and emission efficiency is proposed for each driving condition. Finally, the effects of batteries in initial state of charge and hybridization factor are investigated on HEV performance to evaluate fuel consumption and emissions. Fuel consumption average reduction of about 14% is obtained for optimal configuration data in contrast to default configuration. Also results indicate that proposed controller has reduced emission of about 10% in various traffic conditions.
文摘The paper proposes an adoption of slope,elevation,speed and route distance preview to achieve optimal energymanagement of plug-in hybrid electric vehicles(PHEVs).Theapproach is to identify route features from historical and real-time traffic data,in which information fusion model and trafficprediction model are used to improve the information accuracy.Then,dynamic programming combined with equivalent con-sumption minimization strategy is used to compute an optimalsolution for real-time energy management.The solution is thereference for PHEV energy management control along the route.To improve the system's ability of handling changing situation,the study further explores predictive control model in the real-time control of the energy.A simulation is performed to modelPHEV under above energy control strategy with route preview.The results show that the average fuel consumption of PHEValong the previewed route with model predictive control(MPC)strategy can be reduced compared with optimal strategy andbase control strategy.
基金Project(2007CB209707) supported by the National Basic Research Program of China
文摘In order to achieve the improvement of the driving comfort and energy efficiency,an new e-CVT flexible full hybrid electric system(E2FHS) is proposed,which uses an integrated main drive motor and generator to take the place of the original automatic or manual transmission to realize the functions of continuously variable transmission(e-CVT).The design and prototype realization of the E2FHS system for a plug-in hybrid vehicle(PHEV) is performed.In order to analyze and optimize the parameters and the power flux between different parts of the E2FHS,simulation software is developed.Especially,in order to optimize the performance of the energy economy improvement of the E2FHS,the effect of the different energy management controllers is investigated,and an adaptive online-optimal energy management controller for the E2FHS is built and validated by the prototype PHEV.
文摘The construction and characteristics of a microscale long-optical-path electrochemi- cal cell with a plug-in thin-layer electrode are described.Using ferricyanide as the test species,the thermodynamic parameters of electron transfer processes are determined at car- bon,plantinum,and gold electrodes.
基金Supported by China Automobile Test Cycle Development Project(CATC2015)
文摘A variable parameter self-adaptive control strategy based on driving condition identification is proposed to take full advantage of the fuel saving potential of the plug-in hybrid electric bus(PHEB).Firstly,the principal component analysis(PCA)and the fuzzy c-means clustering(FCM)algorithm is used to construct the comprehensive driving cycle,congestion driving cycle,urban driving cycle and suburban driving cycle of Chinese urban buses.Secondly,an improved particle swarm optimization(IPSO)algorithm is proposed,and is used to optimize the control parameters of PHEB under different driving cycles,respectively.Then,the variable parameter self-adaptive control strategy based on driving condition identification is given.Finally,for an actual running vehicle,the driving condition is identified by relevance vector machine(RVM),and the corresponding control parameters are selected to control the vehicle.The simulation results show that the fuel consumption of using the variable parameter self-adaptive control strategy is reduced by 4.2% compared with that of the fixed parameter control strategy,and the feasibility of the variable parameter self-adaptive control strategy is verified.
文摘In this paper, a plug-in hybrid electrical vehicle(PHEV) is taken as the research object, and its dynamic performance and economic performance are taken as the research goals. Battery charge-sustaining(CS) period is divided into power mode and economy mode. Energy management strategy designing methods of power mode and economy mode are proposed. Maximum velocity, acceleration performance and fuel consumption are simulated during the CS period in the AVL CRUISE simulation environment. The simulation results indicate that the maximum velocity and acceleration time of the power mode are better than those in the economy mode. Fuel consumption of the economy mode is better than that in the power mode. Fuel consumption of PHEV during the CS period is further improved by using the methods proposed in this paper, and this is meaningful for research and development of PHEV.
