Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effec...Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.展开更多
Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chem...Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chemometrics methods has been developed for characterization and discrimination of them in this research.Methods:The polysaccharides were extracted by decocting in water,and then completely hydrolyzed with hydrochloride.Monosaccharides in the hydrolyzates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)for HPLC analysis.More than 20 batches of P.cocos and P.umbellatus from different regions were analyzed.Results:The fingerprints of P.cocos showed five common characteristic peaks,which were identified by comparing with the reference substances.The five peaks corresponded to the derivatives of mannose,ribose,glucose,galactose,and fucose.At the same time,the fingerprints of P.umbellatus showed eight common characteristic peaks,of which seven were identified as the derivatives of mannose,ribose,rhamnose,glucose,galactose,xylose,and fucose.Moreover,the similarity of their fingerprints was respectively calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM published by China Pharmacopoeia Committee(Version 2004 A).And the data were further processed by hierarchical cluster analysis(HCA)and principal component analysis(PCA).The similarity evaluation and HCA indicated that there were no significant difference in P.cocos or P.umbellatus samples from different geographical regions,but PCA was performed to characterize the difference in monosaccharide constituents between P.cocos and P.umbellatus,and linear discriminant analysis(LDA)showed the overall correct classification rate was 100%.Conclusion:The fingerprint analysis method of monosaccharide composition of water-soluble polysaccharides can distinguish P.cocos and P.umbellatus,and can be applied for the authentication or quality control for P.cocos and P.umbellatus.展开更多
目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体...目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体制备时菌丝最适菌龄为72 h,酶液浓度为3%,稳渗剂以0.5 m o l/L的甘露醇为佳。Z10-3原生质体制备时菌丝最适菌龄为56 h,酶液浓度为3%,稳渗剂以0.6 m o l/L的甘露醇为佳。Z10-2和Z10-3原生质体均以甘露醇作稳渗剂再生率较高,其最佳浓度为0.6 m o l/L。在该条件下Z10-2原生质体再生率为7.5×1-0 3,Z10-3为1.3×10-2。展开更多
文摘Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.
基金supported by Shanghai Biotechnology Support Project(Grant No.16401900800)National Science and Technology Project(Grant No.2018ZX09721003-009-011).
文摘Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chemometrics methods has been developed for characterization and discrimination of them in this research.Methods:The polysaccharides were extracted by decocting in water,and then completely hydrolyzed with hydrochloride.Monosaccharides in the hydrolyzates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)for HPLC analysis.More than 20 batches of P.cocos and P.umbellatus from different regions were analyzed.Results:The fingerprints of P.cocos showed five common characteristic peaks,which were identified by comparing with the reference substances.The five peaks corresponded to the derivatives of mannose,ribose,glucose,galactose,and fucose.At the same time,the fingerprints of P.umbellatus showed eight common characteristic peaks,of which seven were identified as the derivatives of mannose,ribose,rhamnose,glucose,galactose,xylose,and fucose.Moreover,the similarity of their fingerprints was respectively calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM published by China Pharmacopoeia Committee(Version 2004 A).And the data were further processed by hierarchical cluster analysis(HCA)and principal component analysis(PCA).The similarity evaluation and HCA indicated that there were no significant difference in P.cocos or P.umbellatus samples from different geographical regions,but PCA was performed to characterize the difference in monosaccharide constituents between P.cocos and P.umbellatus,and linear discriminant analysis(LDA)showed the overall correct classification rate was 100%.Conclusion:The fingerprint analysis method of monosaccharide composition of water-soluble polysaccharides can distinguish P.cocos and P.umbellatus,and can be applied for the authentication or quality control for P.cocos and P.umbellatus.
文摘目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体制备时菌丝最适菌龄为72 h,酶液浓度为3%,稳渗剂以0.5 m o l/L的甘露醇为佳。Z10-3原生质体制备时菌丝最适菌龄为56 h,酶液浓度为3%,稳渗剂以0.6 m o l/L的甘露醇为佳。Z10-2和Z10-3原生质体均以甘露醇作稳渗剂再生率较高,其最佳浓度为0.6 m o l/L。在该条件下Z10-2原生质体再生率为7.5×1-0 3,Z10-3为1.3×10-2。
