Objective:To investigate the mechanism underlying the effects exerted by the Qizhu prescription(QZP)in breast cancer(BC),and the respective targets.Methods: Expression data from the ArrayExpress and The Cancer Genome ...Objective:To investigate the mechanism underlying the effects exerted by the Qizhu prescription(QZP)in breast cancer(BC),and the respective targets.Methods: Expression data from the ArrayExpress and The Cancer Genome Atlas(TCGA)were used to identify differentially expressed genes(DEGs)in BC.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on the DEGs to identify genes involved in protein–protein interactions.Molecular docking was used to explore the dynamic relationship between active molecules and targets.Cell function experiments and animal studies were conducted to evaluate the effects of hub genes and active QZP compounds on BC cell behavior.Results: Among the 25 evaluated BC-related targets of QZP,matrix metalloproteinase-1(MMP1)and epidermal growth factor receptor(EGFR)exhibited the highest degrees of dysregulation.GO and KEGG enrichment analyses revealed that the anti-BC targets of QZP primarily affected drug responses and pathways in cancer cells.Molecular docking analysis suggested potential interactions between EGFR and quercetin/luteolin,as well as between MMP1 and luteolin/kaempferol/quercetin.Quercetin significantly reduced BC cell proliferation,migration,invasion,and tumor development in vivo.Treatment of BC cells with quercetin decreased the expression or activation of several associated proteins.Conclusion: The findings of our study provide new insights into the therapeutic potential of traditional Chinese medicine against BC,with particular reference to QZP.展开更多
Objective:To investigate the active compounds,key targets and molecular mechanism of Qizhu Yuling Decoction in the treatment of esophageal carcinoma based on network pharmacology.Methods:The active compounds of Qizhu ...Objective:To investigate the active compounds,key targets and molecular mechanism of Qizhu Yuling Decoction in the treatment of esophageal carcinoma based on network pharmacology.Methods:The active compounds of Qizhu Yuling Decoction with anti-esophageal cancer activity were screened by TCMSP and literature search,and the drug targets were searched by DrugBank and predicted by SwissTargetPrediction.The esophageal cancer-related genes were obtained from GeneCards,OMIM,DrugBank,TTD,DisGeNET,and the intersection network was selected to obtain candidate genes.The"herb-compound-target-disease"network was constructed with Cytoscape,the PPI network was constructed in STRING and core network modules were screened.Gene ontology and KEGG enrichment analysis was performed by using Metascape,and the"pathway-gene"network was constructed to further screen key targets.Results:A total of 47 active compounds(19 Astragalus,4 Zedoary turmeric,11 Atractylodes macrocephala,9 Curcuma longa,5 Clematis root,and 5 Salvia chinensis),297 candidate genes,2413 GO and 119 KEGG pathways were obtained.Conclusion:The active compounds of Qizhu Yuling Decoction in the treatment of esophageal cancer are quercetin,kaempferol,glycine and ursolic acid.The potential targets are AKT1,MAPK1,MAPK3,PIK3R1 and RELA.GO involves oxidative stress,cell cycle,apoptosis and cell death,and KEGG involves typical cancer pathways,MAPK,NF-κB and PI3K-Akt signaling pathways.This study reveals the molecular biological mechanism of Qizhu Yuling Decoction in the treatment of esophageal cancer,and speculates that the function of potential targets focuses on the interaction of multiple signaling pathways,which can antagonize the proliferation,invasion,metastasis and recurrence of esophageal cancer and improve the prognosis of patients.This study provides new evidence for subsequent new drug development,clinical application and experimental study.展开更多
目的探索芪术肺纤汤治疗特发性肺间质纤维化(idiopathic pulmonary fibrosis,IPF)患者的临床疗效。方法纳入40例2019年10月1日至2021年12月31日于中国中医科学院广安门医院就诊及住院的IPF患者,随机分为试验组和对照组各20例,对照组患...目的探索芪术肺纤汤治疗特发性肺间质纤维化(idiopathic pulmonary fibrosis,IPF)患者的临床疗效。方法纳入40例2019年10月1日至2021年12月31日于中国中医科学院广安门医院就诊及住院的IPF患者,随机分为试验组和对照组各20例,对照组患者口服乙酰半胱氨酸胶囊加百令胶囊,试验组患者在此基础上加用芪术肺纤汤,治疗3个月后观察2组患者肺功能、6分钟步行距离(six-minute walk distance,6MWD)、静息状态血氧饱和度(oxygen saturation,SpO_(2))、圣乔治呼吸问卷(the Saint George's respiratory questionnaire,SGRQ)评分、血清涎液化糖链抗原6(krebs von den lungen-6,KL-6)水平、高分辨CT(high-resolution ct,HRCT)半定量评分变化。结果治疗后试验组患者一氧化碳弥散量占预计值的百分比(carbon monoxide diffusing capacity/predict value,DLCO%)明显高于对照组,差异有统计学意义(P<0.01);治疗后试验组SGRQ总分、症状评分、疾病影响评分、血清KL-6和HRCT半定量评分均低于对照组,差异有统计学意义(P<0.05);肺功能分级和HRCT半定量评分呈正相关性(r=0.849,P<0.01)。结论芪术肺纤汤能够延缓IPF患者的肺纤维化进展,值得临床推广应用。展开更多
基金supported by the National Natural Science Foundation of China(82004240,82104952)Shanghai Municipal Science and Technology Commission Medical Innovation Research Program(21Y11923600)+1 种基金Shanghai Municipal Health Commission Health Industry Clinical Research Specialization(202140172)Shanghai University of Traditional Chinese Medicine Industrial Development Center Healthcare Integration Science and Innovation Project(YYKC-2021-01-153).
文摘Objective:To investigate the mechanism underlying the effects exerted by the Qizhu prescription(QZP)in breast cancer(BC),and the respective targets.Methods: Expression data from the ArrayExpress and The Cancer Genome Atlas(TCGA)were used to identify differentially expressed genes(DEGs)in BC.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on the DEGs to identify genes involved in protein–protein interactions.Molecular docking was used to explore the dynamic relationship between active molecules and targets.Cell function experiments and animal studies were conducted to evaluate the effects of hub genes and active QZP compounds on BC cell behavior.Results: Among the 25 evaluated BC-related targets of QZP,matrix metalloproteinase-1(MMP1)and epidermal growth factor receptor(EGFR)exhibited the highest degrees of dysregulation.GO and KEGG enrichment analyses revealed that the anti-BC targets of QZP primarily affected drug responses and pathways in cancer cells.Molecular docking analysis suggested potential interactions between EGFR and quercetin/luteolin,as well as between MMP1 and luteolin/kaempferol/quercetin.Quercetin significantly reduced BC cell proliferation,migration,invasion,and tumor development in vivo.Treatment of BC cells with quercetin decreased the expression or activation of several associated proteins.Conclusion: The findings of our study provide new insights into the therapeutic potential of traditional Chinese medicine against BC,with particular reference to QZP.
基金National Natural Science Foundation of China(No.81774289)Beijing Science and Technology Plan Major Fund Project(No.D161100005116004)China Academy of Chinese Medical Sciences(No.ZZ11-028)。
文摘Objective:To investigate the active compounds,key targets and molecular mechanism of Qizhu Yuling Decoction in the treatment of esophageal carcinoma based on network pharmacology.Methods:The active compounds of Qizhu Yuling Decoction with anti-esophageal cancer activity were screened by TCMSP and literature search,and the drug targets were searched by DrugBank and predicted by SwissTargetPrediction.The esophageal cancer-related genes were obtained from GeneCards,OMIM,DrugBank,TTD,DisGeNET,and the intersection network was selected to obtain candidate genes.The"herb-compound-target-disease"network was constructed with Cytoscape,the PPI network was constructed in STRING and core network modules were screened.Gene ontology and KEGG enrichment analysis was performed by using Metascape,and the"pathway-gene"network was constructed to further screen key targets.Results:A total of 47 active compounds(19 Astragalus,4 Zedoary turmeric,11 Atractylodes macrocephala,9 Curcuma longa,5 Clematis root,and 5 Salvia chinensis),297 candidate genes,2413 GO and 119 KEGG pathways were obtained.Conclusion:The active compounds of Qizhu Yuling Decoction in the treatment of esophageal cancer are quercetin,kaempferol,glycine and ursolic acid.The potential targets are AKT1,MAPK1,MAPK3,PIK3R1 and RELA.GO involves oxidative stress,cell cycle,apoptosis and cell death,and KEGG involves typical cancer pathways,MAPK,NF-κB and PI3K-Akt signaling pathways.This study reveals the molecular biological mechanism of Qizhu Yuling Decoction in the treatment of esophageal cancer,and speculates that the function of potential targets focuses on the interaction of multiple signaling pathways,which can antagonize the proliferation,invasion,metastasis and recurrence of esophageal cancer and improve the prognosis of patients.This study provides new evidence for subsequent new drug development,clinical application and experimental study.
文摘目的探索芪术肺纤汤治疗特发性肺间质纤维化(idiopathic pulmonary fibrosis,IPF)患者的临床疗效。方法纳入40例2019年10月1日至2021年12月31日于中国中医科学院广安门医院就诊及住院的IPF患者,随机分为试验组和对照组各20例,对照组患者口服乙酰半胱氨酸胶囊加百令胶囊,试验组患者在此基础上加用芪术肺纤汤,治疗3个月后观察2组患者肺功能、6分钟步行距离(six-minute walk distance,6MWD)、静息状态血氧饱和度(oxygen saturation,SpO_(2))、圣乔治呼吸问卷(the Saint George's respiratory questionnaire,SGRQ)评分、血清涎液化糖链抗原6(krebs von den lungen-6,KL-6)水平、高分辨CT(high-resolution ct,HRCT)半定量评分变化。结果治疗后试验组患者一氧化碳弥散量占预计值的百分比(carbon monoxide diffusing capacity/predict value,DLCO%)明显高于对照组,差异有统计学意义(P<0.01);治疗后试验组SGRQ总分、症状评分、疾病影响评分、血清KL-6和HRCT半定量评分均低于对照组,差异有统计学意义(P<0.05);肺功能分级和HRCT半定量评分呈正相关性(r=0.849,P<0.01)。结论芪术肺纤汤能够延缓IPF患者的肺纤维化进展,值得临床推广应用。
