Simplified quantitative analysis method and its application in the insitu synthesized copper-based azide chips

Copper-based azide(Cu(N_(3))2 or CuN_(3),CA)chips synthesized by in-situ azide reaction and utilized in miniaturized explosive systems has become a hot research topic in recent years.However,the advantages of in-situ synthesis method,including small size and low dosage,bring about difficulties in quantitative analysis and differences in ignition capabilities of CA chips.The aim of present work is to develop a simplified quantitative analysis method for accurate and safe analysis of components in CA chips to evaluate and investigate the corresponding ignition ability.In this work,Cu(N_(3))2 and CuN_(3)components in CA chips were separated through dissolution and distillation by utilizing the difference in solubility and corresponding content was obtained by measuring N_(3)-concentration through spectrophotometry.The spectrophotometry method was optimized by studying influencing factors and the recovery rate of different separation methods was studied,ensuring the accuracy and reproducibility of test results.The optimized method is linear in range from 1.0-25.0 mg/L,with a correlation coefficient R^(2)=0.9998,which meets the requirements of CA chips with a milligram-level content test.Compared with the existing ICP method,component analysis results of CA chips obtained by spectrophotometry are closer to real component content in samples and have satisfactory accuracy.Moreover,as its application in miniaturized explosive systems,the ignition ability of CA chips with different component contents for direct ink writing CL-20 and the corresponding mechanism was studied.This study provided a basis and idea for the design and performance evaluation of CA chips in miniaturized explosive systems.

Incorporating empirical knowledge into data-driven variable selection for quantitative analysis of coal ash content by laser-induced breakdown spectroscopy

Laser-induced breakdown spectroscopy(LIBS)has become a widely used atomic spectroscopic technique for rapid coal analysis.However,the vast amount of spectral information in LIBS contains signal uncertainty,which can affect its quantification performance.In this work,we propose a hybrid variable selection method to improve the performance of LIBS quantification.Important variables are first identified using Pearson's correlation coefficient,mutual information,least absolute shrinkage and selection operator(LASSO)and random forest,and then filtered and combined with empirical variables related to fingerprint elements of coal ash content.Subsequently,these variables are fed into a partial least squares regression(PLSR).Additionally,in some models,certain variables unrelated to ash content are removed manually to study the impact of variable deselection on model performance.The proposed hybrid strategy was tested on three LIBS datasets for quantitative analysis of coal ash content and compared with the corresponding data-driven baseline method.It is significantly better than the variable selection only method based on empirical knowledge and in most cases outperforms the baseline method.The results showed that on all three datasets the hybrid strategy for variable selection combining empirical knowledge and data-driven algorithms achieved the lowest root mean square error of prediction(RMSEP)values of 1.605,3.478 and 1.647,respectively,which were significantly lower than those obtained from multiple linear regression using only 12 empirical variables,which are 1.959,3.718 and 2.181,respectively.The LASSO-PLSR model with empirical support and 20 selected variables exhibited a significantly improved performance after variable deselection,with RMSEP values dropping from 1.635,3.962 and 1.647 to 1.483,3.086 and 1.567,respectively.Such results demonstrate that using empirical knowledge as a support for datadriven variable selection can be a viable approach to improve the accuracy and reliability of LIBS quantification.

Research on batch multielement rapid quantitative analysis based on the standard curve-assisted calibration-free laserinduced breakdown spectroscopy method

This study proposes a batch rapid quantitative analysis method for multiple elements by combining the advantages of standard curve(SC)and calibration-free laser-induced breakdown spectroscopy(CF-LIBS)technology to achieve synchronous,rapid,and accurate measurement of elements in a large number of samples,namely,SC-assisted CF-LIBS.Al alloy standard samples,divided into calibration and test samples,were applied to validate the proposed method.SC was built based on the characteristic line of Pb and Cr in the calibration sample,and the contents of Pb and Cr in the test sample were calculated with relative errors of 6%and 4%,respectively.SC built using Cr with multiple characteristic lines yielded better calculation results.The relative contents of ten elements in the test sample were calculated using CF-LIBS.Subsequently,the SC-assisted CF-LIBS was executed,with the majority of the calculation relative errors falling within the range of 2%-5%.Finally,the Al and Na contents of the Al alloy were predicted.The results demonstrate that it effectively enables the rapid and accurate quantitative analysis of multiple elements after a single-element SC analysis of the tested samples.Furthermore,this quantitative analysis method was successfully applied to soil and Astragalus samples,realizing an accurate calculation of the contents of multiple elements.Thus,it is important to advance the LIBS quantitative analysis and its related applications.

Spectra-assisted laser focusing in quantitative analysis of laser-induced breakdown spectroscopy for copper alloys

Laser-induced breakdown spectroscopy(LIBS)is a capable technique for elementary analysis,while LIBS quantitation is still under development.In quantitation,precise laser focusing plays an important role because it ensures the distance between the laser and samples.In the present work,we employed spectral intensity as a direct way to assist laser focusing in LIBS quantitation for copper alloys.It is found that both the air emission and the copper line could be used to determine the position of the sample surface by referencing the intensity maximum.Nevertheless,the fine quantitation was only realized at the position where the air emission(e.g.O(I)777.4 nm)reached intensity maximum,and also in this way,a repeatable quantitation was successfully achieved even after 120 days.The results suggested that the LIBS quantitation was highly dependent on the focusing position of the laser,and spectra-assisted focusing could be a simple way to find the identical condition for different samples’detection.In the future,this method might be applicable in field measurements for LIBS analysis of solids.

Energy-dispersive X-ray Spectroscopy for the Quantitative Analysis of Pyrite Thin Specimens

To explore ways to improve the accuracy of quantitative analysis of samples in the micrometer to nanometer range of magnitudes,we adopted analytical transmission electron microscopy(AEM/EDS)for qualitative and quantitative analysis of pyrite materials.Additionally,the k factor of pyrite is calculated experimentally.To develop an appropriate non-standard quantitative analysis model for pyrite materials,the experimentally calculated k factor is compared with that estimated from the non-standard quantitative analytical model of the instrument software.The experimental findings demonstrate that the EDS attached to a TEM can be employed for precise quantitative analysis of micro-and nanoscale regions of pyrite materials.Furthermore,it serves as a reference for improving the results of the EDS quantitative analysis of other sulfides.

Qualitative and Quantitative Analysis of Alkaloids in Cortex Phellodendri by HPLC-ESI-MS/MS and HPLC-DAD

A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer（HPLC-ESI-MS/MS） coupled with a photodiode array detector（HPLC-DAD） and principal component analysis（PCA） was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri（CP） samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis（CPC） and Cortex Phellodendri Amurensis（CPA）. Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC（58.75 mg/g） was higher than that in CPA（9.16 mg/g）, while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, pahnatine, phellodendrine and magnoflorine contribute most to the above mentioned calssifying . The proposed method oroved to be a useful tool in the aualitv control of Chinese herbal medicines.

Change of choline compounds in sodium selenite-induced apoptosis of rats used as quantitative analysis by in vitro 9.4T MR spectroscopy

AIM: To study liver cell apoptosis caused by the toxicity of selenium and observe the alteration of choline compounds using in vitro 9.4T high resolution magnetic resonance spectroscopy. METHODS: Twenty male Wistar rats were randomly divided into two groups. The rats in the treatment group were intraperitoneally injected with sodium selenite and the control group with distilled water. All rats were sacrifi ced and the livers were dissected. 1H-MRS data were collected using in vitro 9.4T high resolution magnetic resonance spectrometer. Spectra were processed using XWINNMR and MestRe-c 4.3. HE and TUNEL staining was employed to detect and confi rm the change of liver cells. RESULTS: Good 1H-MR spectra of perchloric acid extract from liver tissue of rats were obtained. The conventional metabolites were detected and assigned. Concentrations of different ingredient choline compounds in treatment group vs control group were as follows: total choline compounds,5.08 ± 0.97 mmol/L vs 3.81 ± 1.16 mmol/L (P = 0.05); and free choline,1.07 ± 0.23 mmol/L vs 0.65 ± 0.20 mmol/L (P = 0.00). However,there was no statistical signif icance between the two groups. The hepatic sinus and cellular structure of hepatic cells in treatmentgroup were abnormal. Apoptosis of hepatic cells was confi rmed by TUNEL assay. CONCLUSION: High dose selenium compounds can cause the rat liver lesion and induce cell apoptosis in vivo. High resolution 1H-MRS in vitro can detect diversified metabolism. The changing trend for different ingredient of choline compounds is not completely the same at early period of apoptosis.

Quantitative Analysis of Moisture Effect on Black Soil Reflectance

Several studies have demonstrated that soil reflectance decreases with increasing soil moisture content, or increases when the soil moisture reaches a certain content; however, there are few analyses on the quantitative relationship between soil reflectance and its moisture, especially in the case of black soils in northeast China. A new moisture adjusting method was developed to obtain soil reflectance with a smaller moisture interval to describe the quantitative relationship between soil reflectance and moisture. For the soil samples with moisture contents ranging from air-dry to saturated, the changes in soil reflectance with soil moisture can be depicted using a cubic equation. Both moisture threshold (MT) and moisture inflexion (MI) of soil reflectance can also be determined by the equation. When the moisture range was smaller than MT, soil reflectance can be simulated with a linear model. However, for samples with different soil organic matter (OM), the parameters of the linear model varied regularly with the OM content. Based on their relationship, the soil moisture can be estimated from soil reflectance in the black soil region.

Quantitative analysis of the dynamic change and spatial differences of the ecological security: a case study of Loess Plateau in northern Shaanxi Province

Using the theory and method of the ecological footprint, and combining the changes of regional land use, resource environment, population, society and economy, this paper calculated the ecological footprint, ecological carrying capacity and ecological surplus/loss in 1986-2002 on the Loess Plateau in northern Shaanxi Province. What is more, this paper has put forward the concept of ecological pressure index, set up ecological pressure index models, and ecological security grading systems, and the prediction models of different ecological footprints, ecological carrying capacity, ecological surplus and ecological safety change, and also has assessed the ecological footprint demands of 10,000 yuan GDE The results of this study are as follows： （1） the ecological carrying capacity in northern Shaanxi shows a decreasing trend, the difference of reducing range is the fastest; （2） the ecological footprint appears an increasing trend; （3） ecological pressure index rose to 0.91 from 0.44 during 1986-2002 on the Loess Plateau of northern Shaanxi with an increase of 47%; and （4） the ecological security in the study area is in a critical state, and the ecological oressure index has been increasing rapidlv.

A quantitative analysis method for GPR signals based on optimal biorthogonal wavelet

Due to the disturbances arising from the coherence of reflected waves and from echo noise,problems such as limitations,instability and poor accuracy exist with the current quantitative analysis methods.According to the intrinsic features of GPR signals and wavelet time–frequency analysis,an optimal wavelet basis named GPR3.3 wavelet is constructed via an improved biorthogonal wavelet construction method to quantitatively analyse the GPR signal.A new quantitative analysis method based on the biorthogonal wavelet(the QAGBW method)is proposed and applied in the analysis of analogue and measured signals.The results show that compared with the Bayesian frequency-domain blind deconvolution and with existing wavelet bases,the QAGBW method based on optimal wavelet can limit the disturbance from factors such as the coherence of reflected waves and echo noise,improve the quantitative analytical precision of the GPR signal,and match the minimum thickness for quantitative analysis with the vertical resolution of GPR detection.

Calculation of Ecological Footprint Based on Modified Method and Quantitative Analysis of Its Impact Factors——A Case Study of Shanghai

Ecological footprint （EF） has attracted much attention internationally as a potential indicator for sustainable development. In China, EF has also gained recognition in related fields and disciplines. Moreover, some scholars in these fields have proposed alternative methods to calculate EF and have made some progress. This paper, therefore, begins with the introduction and development of EF in China. However, the established methods of EF calculation hold some limitations, such as indicator variance and result abnormality. In order to resolve those problems, the authors make a further modification considering the demand of EF as a comprehensive indicator： 1） More accurate analysis has been done to divide EF into several parts： imported EF, exported EF, and producible EF, which is the solution to the problem of abnormality in original EF results and can explain abnormal phenomena reasonably. 2） Considering the actual situa- tion of Shanghai, emended equivalence factor is brought forward and a matrix is formed with equivalence factors. The measure can reduce the deviation between the fact and the results. 3） The calculation compares local yield with global average yield to analyze the effects of yields. And based on local yields in different years, the results are more accurate. Finally, the calculation method is applied to calculating EF of Shanghai from 1980 to 2003, and the subsequent detailed analysis is presented. Available data and results suggest a statistically significant correlation coefficient between EF and GDP, population density and urbaniTation level. Through analyzing the process of calculating EF and its results, it can be seen that EF, as a macro-indicator, can not exactly indicate whether development within a region can meet the re- quests of sustainable development, which can be explained by the fact that the result of EF is impacted greatly by sub- jective factors including national policy,available technology, population, etc. Nevertheless, EF can demonstrate, at least to some degree, the regional status in terms of resources and energy consumption, as well as developmental potential. The calculation of EF, therefore, deserves further research to achieve more far-reaching significance in application.

Nondestructive Quantitative Analysis of Cofrel Medicines by Double ANN-NIR Spectroscopy

In this paper, a double artificial neural network （DANN） algorithm was used to parse near infrared （NIR） reflectance spectrum of Cofrel medicines. The contents of benproperine phosphate, which is the effective ingredient in Cofrel medicines, were accurately nondestructive quantitatively predicted. Compared the results with those of HPLC, the relative errors （RE %） were less than 0.18%. The analytical results could be applied to qualitative control of Cofrel medicines.

A feature selection method combined with ridge regression and recursive feature elimination in quantitative analysis of laser induced breakdown spectroscopy

In the spectral analysis of laser-induced breakdown spectroscopy,abundant characteristic spectral lines and severe interference information exist simultaneously in the original spectral data.Here,a feature selection method called recursive feature elimination based on ridge regression(Ridge-RFE)for the original spectral data is recommended to make full use of the valid information of spectra.In the Ridge-RFE method,the absolute value of the ridge regression coefficient was used as a criterion to screen spectral characteristic,the feature with the absolute value of minimum weight in the input subset features was removed by recursive feature elimination(RFE),and the selected features were used as inputs of the partial least squares regression(PLS)model.The Ridge-RFE method based PLS model was used to measure the Fe,Si,Mg,Cu,Zn and Mn for 51 aluminum alloy samples,and the results showed that the root mean square error of prediction decreased greatly compared to the PLS model with full spectrum as input.The overall results demonstrate that the Ridge-RFE method is more efficient to extract the redundant features,make PLS model for better quantitative analysis results and improve model generalization ability.

Computer Management of Quantitative Analysis Chemistry Experiment

The application of computer in the quantitative analysis chemistry experiment is a chemistry experiment teaching applications software, developed by Visual Basic （6.0）, based on the content of quantitative analysis chemistry experiment of chemistry major in higher institute. This software has the function of the automatic processing the experimental data, the automatic generation of test report copies, and the automatic evaluation of students＇ experimental results, which solve the reliability, objectivity and accuracy problems of the students＇ experiment data processing and evaluation, and avoid interference with human factors. The software has the characteristic of the easy installation, the easy operation, the strong practicability, pertinence, the systematicness and the running stability, so it provides a platform in the quantitative analysis chemistry experiment for the students＇ assessment system of automatic processing, and it has a high popularization value. The project＇s technical route design is reasonable, the research method is correct, and the experimental data processing results are reliable, which has reached the leading domestic level in the quantitative analysis chemistry experiment teaching field of computer data processing. And this project has been through the achievements appraisal of Gansu Provincial Sci. ＆ Tech. Department.

iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lac- tation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by ProteoMiner beads, protein labeling by isobaric tags for relative and absolute quantification, and protein identification by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were asso- ciated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V, a2-antiplasmin and prothrombin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inflammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.

Rietveld Quantitative Analysis of Carbides Precipitation in Normalized-tempered 2.25Cr-1Mo-0.25V Steel

The quantitative determination of the mass fractions of precipitates in steels is very difficult using traditional materials characterization techniques. The Rietveld full-pattern fitting algorithm was introduced to solve this problem. The precipitated multicomponents’ mass fraction of M3C, MC, M7C3 and M23C6 were evaluated precisely and relatively quickly. It is found evolution of carbides apparently occurs during tempering at high temperatures, and a two-step transformation mechanism is proposed for M7C3 during early tempering treatment. The method is an effective way on the investigation of precipitation kinetics, which may play a promising role in propertities’ enhancement and design of the heat-resistant steels.

Quantitative analysis of globularization and modeling of TC17 alloy with basketweave microstructure

The globularization behavior and mechanism of TC17 alloy with basketweave microstructure were investigated, and the models of dynamic and static globularization kinetics were established. The quantitative and metallographic results show that the globularization of α phase is sensitive to the parameters of deformation and heat treatment. By EBSD analysis, the formation and evolution mechanisms of intra-α boundaries are related to discontinuous dynamic recrystallization and continuous dynamic recrystallization, which can form α grains with high and low misorientations between neighbour grains after the heat treatment, respectively. Based on the globularization behavior and mechanism, two modified JMAK models are developed to predict the dynamic and static globularization kinetics, and the mean absolute relative errors(MARE) of 10.67% and 13.80% indicate the accuracy of the dynamic and static globularization kinetics models. The results of this work can provide guidance for controlling microstructure of titanium alloy.

Quantitative Analysis of Micro-porosity of Eco-material by Using SEM Technique

Microstructure of the eco-material combining vegetation recovery with slope protection is important for determining plant-growing properties.Several techniques for analyzing the eco-material microstructure are presented,including the freeze-cut-drying method of preparing samples for scanning electronic microscopy (SEM),the SEM image processing technique and quantifying analysis method of the SEM images,and etc.The aggregates and pores in SEM images are identified using the different mathematics operators,and their effects are compared.The areas of aggregates and pores are obtained using the operator of morphology,and the influences of different thresholds in image segmentation are also discussed.The results show that the method,in which the variation of non-maximum grey-level gradient is limited,improves the effect of edge detections due to a weak distinction existing at the edge between the aggregates and pores in image.The determination of the threshold should combine the image characteristic with filling operation,so as to assure the precision of the image analysis,in which the contact-segmentation is the simplest and most effective method.The results also show that the pore areas in eco-materials are generally larger than those in the correlative soils,and their increment is large as soil fabric being fine.These differences are related to admixture of expansive perlitic.The operator of morphology provides a new method for the image analysis of other porous material microstructure such as soils and concretes.

Quantitative Analysis of Heavy Metals in Water Based on LIBS with an Automatic Device for Sample Preparation

Abstract Heavy metals in water can be deposited on graphite flakes, which can be used as an enrichment method for laser-induced breakdown spectroscopy （LIBS） and is studied in this paper. The graphite samples were prepared with an automatic device, which was composed of a loading and unloading module, a quantitatively adding solution module, a rapid heating and drying module and a precise rotating module. The experimental results showed that the sample preparation methods had no significant effect on sample distribution and the LIBS signal accumulated in 20 pulses was stable and repeatable. With an increasing amount of the sample solution on the graphite flake, the peak intensity at Cu I 324.75 nm accorded with the exponential function with a correlation coefficient of 0.9963 and the background intensity remained unchanged. The limit of detection （LOD） was calculated through linear fitting of the peak intensity versus the concentration. The LOD decreased rapidly with an increasing amount of sample solution until the amount exceeded 20 mL and the correlation coefficient of exponential function fitting was 0.991. The LOD of Pb, Ni, Cd, Cr and Zn after evaporating different amounts of sample solution on the graphite flakes was measured and the variation tendency of their LOD with sample solution amounts was similar to the tendency for Cu. The experimental data and conclusions could provide a reference for automatic sample preparation and heavy metal in situ detection.

Enhancing mechanism of intestinal absorption of highly lipophilic compounds using microemulsion e Quantitative analysis of the partitioning to the mesenteric lymph in intestinal cells

The purpose of this study was to quantify the effect of the fatty acid alkyl-chain length of a polyethylene glycol(PEG)glyceryl ester,which was used as a microemulsion oil component,on the partitioning of highly lipophilic compounds to the mesenteric lymph after oral administration.Oil blue N,a highly lipophilic anthraquinone derivative,was orally administered to lymph duct-cannulated and untreated rats in two kinds of different microemulsions.Gelucire®50/13 and Gelucire®44/14 were used as the oil component with long chain and medium chain fatty acid portions,respectively,of PEG glyceryl esters in microemulsions.The cumulative amount of oil blue N in lymph fluid was almost the same between the two microemulsions,although the transferred amount of oil component(triglyceride)in the lymph after administration of the Gelucire®50/13 microemulsion was significantly higher than that of the Gelucire®44/14 microemulsion.On the other hand,the solubility of oil blue N in Gelucire®44/14 was much higher than that in Gelucire®50/13.No significant differences were observed between microemulsions in the bioavailability of oil blue N.From these data,the partitioning of oil blue N to the lymph was calculated using a mathematical model,showing that the partitioning ratios of oil blue N to the lymph fluid were almost the same for both microemulsions.The solubility of oil blue N to the oil component of the microemulsions and the transfer of triglycerides to the lymph after administration of the microemulsions counteract each other,leading to similar partitioning ratios of oil blue N to the lymph.