Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-asso...Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis pigmentosa.The aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal function.To do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and immunofluorescence.Data-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western blotting.AAV2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 mice.Differentially expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type mice.Kyoto Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction pathways.Furthermore,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B treatment.Finally,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B treatment.Our data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upregulating Bcl-2/Bax expression in retinitis pigmentosa.展开更多
It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly eval...It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly evaluated and calculated via the classification and regression neural networks. An efficient databasegeneration method is developed for obtaining eight types of free return orbits and then the RD is defined by the orbit’s inclination and right ascension of ascending node(RAAN) at the perilune. A classify neural network and a regression network are trained respectively. The former is built for classifying the type of the RD, and the latter is built for calculating the inclination and RAAN of the RD. The simulation results show that two neural networks are well trained. The classification model has an accuracy of more than 99% and the mean square error of the regression model is less than 0.01°on the test set. Moreover, a serial strategy is proposed to combine the two surrogate models and a recognition tool is built to evaluate whether a lunar site could be reached. The proposed deep learning method shows the superiority in computation efficiency compared with the traditional double two-body model.展开更多
6月18日,在拜访了协同电子稍事休息后,朋友为我安排的是涩谷唱片之旅。先是到了两家disk union二手唱片店和一家TOWERRECORDS唱片门店,随意淘了几张CD唱片。然后,我们来到涩谷地铁站附近的日本料理“长野屋”打卡。料理好肚子,我们穿过...6月18日,在拜访了协同电子稍事休息后,朋友为我安排的是涩谷唱片之旅。先是到了两家disk union二手唱片店和一家TOWERRECORDS唱片门店,随意淘了几张CD唱片。然后,我们来到涩谷地铁站附近的日本料理“长野屋”打卡。料理好肚子,我们穿过涩谷著名的打卡点“涩谷十字路口”,按约定时间来到著名的TOWER RECORDS SHIBUYA。展开更多
Two common problems for a typical Power distribution system are voltage collapse & instability. Challenge is to identify the vulnerable nodes and apply the effective corrective actions. This paper presents a proba...Two common problems for a typical Power distribution system are voltage collapse & instability. Challenge is to identify the vulnerable nodes and apply the effective corrective actions. This paper presents a probabilistic fuzzy approach to assess the node status and proposes feeder reconfiguration as a method to address the same. Feeder reconfiguration is altering the topological structures of distribution feeders by changing the open/closed states of the sectionalizing and ties switches. The solution is converge using a probabilistic fuzzy modeled solution, which defines the nodal vulnerability index (VI) as a function of node voltage and node voltage stability index and predicts nodes critical to voltage collapse. The information is further used to plan best combination of feeders from each loop in distribution system to be switched out such that the resulting configuration gives the optimal performance i.e. best voltage profile and minimal kW losses. The proposed method is tested on established radial distribution system and results are presented.展开更多
Nano-materials are the newly emerged materials developed since the end ofeighties and the beginning of nineties of last century by a frontier and cross-disciplinetechnology-nanotechnology. Nanomaterial research involv...Nano-materials are the newly emerged materials developed since the end ofeighties and the beginning of nineties of last century by a frontier and cross-disciplinetechnology-nanotechnology. Nanomaterial research involves many unknown processes and wonderfulphenomena, which can hardly been explained using traditional terminology and standards. To guide andstandardize the development of nanomaterials, in the year of 2002, the Ministry of Science andTechnology listed the project of developing the 'Nanomaterial Standard' as the 'Basic ResearchProgram'. Meanwhile, the Standardization Administration of China (SAC) also listed the developmentof 'Nanomaterial Standards' in its yearly work program. Through the efforts of the drafting bodies,the work of drafting and developing the standards has been partly completed. Now, 7 nationalstandards on nanomaterials headed by Terminology for nanomterials (GB/T 19619-2004) and etc. havebeen promulgated by SAC on Sept.29*, 2004 and Dqc.1T, 2004 and were implemented since April 1st,2005. This is the first time China approves and promulgates national standards concerningnanomaterials and it is also the first time in the world that nanomaterials standards arepromulgated as the form of national standards. On Feb. 28th, 2005, the General Administration ofQuality Supervision, Inspection and Quarantine (AQSIQ) and SAC jointly held a press conferenceannouncing the promulgation of the first batch of national standards on nanomaterials in Beijing.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82071008(to BL)and 82004001(to XJ)Medical Science and Technology Program of Health Commission of Henan Province,No.LHGJ20210072(to RQ)Science and Technology Department of Henan Province,No.212102310307(to XJ)。
文摘Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis pigmentosa.The aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal function.To do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and immunofluorescence.Data-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western blotting.AAV2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 mice.Differentially expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type mice.Kyoto Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction pathways.Furthermore,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B treatment.Finally,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B treatment.Our data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upregulating Bcl-2/Bax expression in retinitis pigmentosa.
基金supported by the National Natural Science Foundation of China (12072365)the Natural Science Foundation of Hunan Province of China (2020JJ4657)。
文摘It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly evaluated and calculated via the classification and regression neural networks. An efficient databasegeneration method is developed for obtaining eight types of free return orbits and then the RD is defined by the orbit’s inclination and right ascension of ascending node(RAAN) at the perilune. A classify neural network and a regression network are trained respectively. The former is built for classifying the type of the RD, and the latter is built for calculating the inclination and RAAN of the RD. The simulation results show that two neural networks are well trained. The classification model has an accuracy of more than 99% and the mean square error of the regression model is less than 0.01°on the test set. Moreover, a serial strategy is proposed to combine the two surrogate models and a recognition tool is built to evaluate whether a lunar site could be reached. The proposed deep learning method shows the superiority in computation efficiency compared with the traditional double two-body model.
文摘6月18日,在拜访了协同电子稍事休息后,朋友为我安排的是涩谷唱片之旅。先是到了两家disk union二手唱片店和一家TOWERRECORDS唱片门店,随意淘了几张CD唱片。然后,我们来到涩谷地铁站附近的日本料理“长野屋”打卡。料理好肚子,我们穿过涩谷著名的打卡点“涩谷十字路口”,按约定时间来到著名的TOWER RECORDS SHIBUYA。
文摘Two common problems for a typical Power distribution system are voltage collapse & instability. Challenge is to identify the vulnerable nodes and apply the effective corrective actions. This paper presents a probabilistic fuzzy approach to assess the node status and proposes feeder reconfiguration as a method to address the same. Feeder reconfiguration is altering the topological structures of distribution feeders by changing the open/closed states of the sectionalizing and ties switches. The solution is converge using a probabilistic fuzzy modeled solution, which defines the nodal vulnerability index (VI) as a function of node voltage and node voltage stability index and predicts nodes critical to voltage collapse. The information is further used to plan best combination of feeders from each loop in distribution system to be switched out such that the resulting configuration gives the optimal performance i.e. best voltage profile and minimal kW losses. The proposed method is tested on established radial distribution system and results are presented.
文摘Nano-materials are the newly emerged materials developed since the end ofeighties and the beginning of nineties of last century by a frontier and cross-disciplinetechnology-nanotechnology. Nanomaterial research involves many unknown processes and wonderfulphenomena, which can hardly been explained using traditional terminology and standards. To guide andstandardize the development of nanomaterials, in the year of 2002, the Ministry of Science andTechnology listed the project of developing the 'Nanomaterial Standard' as the 'Basic ResearchProgram'. Meanwhile, the Standardization Administration of China (SAC) also listed the developmentof 'Nanomaterial Standards' in its yearly work program. Through the efforts of the drafting bodies,the work of drafting and developing the standards has been partly completed. Now, 7 nationalstandards on nanomaterials headed by Terminology for nanomterials (GB/T 19619-2004) and etc. havebeen promulgated by SAC on Sept.29*, 2004 and Dqc.1T, 2004 and were implemented since April 1st,2005. This is the first time China approves and promulgates national standards concerningnanomaterials and it is also the first time in the world that nanomaterials standards arepromulgated as the form of national standards. On Feb. 28th, 2005, the General Administration ofQuality Supervision, Inspection and Quarantine (AQSIQ) and SAC jointly held a press conferenceannouncing the promulgation of the first batch of national standards on nanomaterials in Beijing.
