目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患...目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组,相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53m RNA相对表达量,用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。结果:实验组RBM38 m RNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于对照组(0.271±0.128、0.206±0.099),实验组p53 m RNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于对照组(0.308±0.167、0.332±0.071),差异均有统计学意义(均P<0.01)。RBM38表达与肺腺癌患者TNM分期、浸润深度有关(P<0.05),p53表达与患者TNM分期有关(P<0.05)。实验组RBM38与p53蛋白表达呈负相关(r=-0.626,P<0.01)。结论:肺腺癌组织RBM38、p53 m RNA及蛋白表达均高于癌旁组织,两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少,RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展,RBM38可能成为肺腺癌分子靶向治疗的靶点。展开更多
During vegetative development, higher plants continuously form new leaves in regular spatial and temporal patterns. Mutants with abnormal leaf developmental patterns not only provide a great insight into understanding...During vegetative development, higher plants continuously form new leaves in regular spatial and temporal patterns. Mutants with abnormal leaf developmental patterns not only provide a great insight into understanding the regulatory mechanism of plant architecture, but also enrich the ways to its modification by which crop yield could be improved. Here, we reported the characterization of the rice leafy-head2 （lhd2） mutant that exhibits shortened plastochron, dwarfism, reduced tiller number, and failure of phase transition from vegetative to reproductive growth. Anatomical and histological study revealed that the rapid emergence of leaves in lhd2 was resulted from the rapid initiation of leaf primordia whereas the reduced tiller number was a consequence of the suppression of the tiller bud outgrowth. The molecular and genetic analysis showed that LHD2 encodes a putative RNA binding protein with 67% similarity to maize TEl. Comparison of genome-scale expression profiles between wild-type and lhd2 plants suggested that LHD2 may regulate rice shoot development through KNOXand hormone-related genes. The similar phenotypes caused by LHD2 mutation and the conserved expression pattern of LHD2 indicated a conserved mechanism in controlling the temporal leaf initiation in grass.展开更多
文摘目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组,相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53m RNA相对表达量,用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。结果:实验组RBM38 m RNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于对照组(0.271±0.128、0.206±0.099),实验组p53 m RNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于对照组(0.308±0.167、0.332±0.071),差异均有统计学意义(均P<0.01)。RBM38表达与肺腺癌患者TNM分期、浸润深度有关(P<0.05),p53表达与患者TNM分期有关(P<0.05)。实验组RBM38与p53蛋白表达呈负相关(r=-0.626,P<0.01)。结论:肺腺癌组织RBM38、p53 m RNA及蛋白表达均高于癌旁组织,两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少,RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展,RBM38可能成为肺腺癌分子靶向治疗的靶点。
文摘During vegetative development, higher plants continuously form new leaves in regular spatial and temporal patterns. Mutants with abnormal leaf developmental patterns not only provide a great insight into understanding the regulatory mechanism of plant architecture, but also enrich the ways to its modification by which crop yield could be improved. Here, we reported the characterization of the rice leafy-head2 （lhd2） mutant that exhibits shortened plastochron, dwarfism, reduced tiller number, and failure of phase transition from vegetative to reproductive growth. Anatomical and histological study revealed that the rapid emergence of leaves in lhd2 was resulted from the rapid initiation of leaf primordia whereas the reduced tiller number was a consequence of the suppression of the tiller bud outgrowth. The molecular and genetic analysis showed that LHD2 encodes a putative RNA binding protein with 67% similarity to maize TEl. Comparison of genome-scale expression profiles between wild-type and lhd2 plants suggested that LHD2 may regulate rice shoot development through KNOXand hormone-related genes. The similar phenotypes caused by LHD2 mutation and the conserved expression pattern of LHD2 indicated a conserved mechanism in controlling the temporal leaf initiation in grass.
