Objective: To explain the phenomenon that Panax ginseng is not compatible with Raphani Semen based on pharmacodynamics and pharmacokinetics.Methods: The forced swimming time and biochemical parameters such as blood la...Objective: To explain the phenomenon that Panax ginseng is not compatible with Raphani Semen based on pharmacodynamics and pharmacokinetics.Methods: The forced swimming time and biochemical parameters such as blood lactate(BLA), serum urea nitrogen(SUN), and hepatic glycogen(GLU) were determined for anti-fatigue experiment. The UPLCMS/MS was used to analyze the pharmacokinetics of Rg1, Re, Rb1, and Rd after orally administration of P. ginseng and P. ginseng combined with Raphani Semen to rats. Pharmacokinetic differences of four ginsenosides between single uses of P. ginseng and combined with Raphani Semen were investigated.Results: The results showed that Raphani Semen tended to significantly reduce the anti-fatigue activity of P. ginseng. Co-administration of P. ginseng and Raphani Semen had significant effects on the pharmacokinetics of the four ginsenosides in rats compared to that observed with P. ginseng extract alone. The AUC0–12 hvalues of the four ginsenosides in PG group were higher than the corresponding values in the PR group. It can be inferred that Raphani Semen decreased the blood exposure of the four ginsenosides in rats when it combined with P. ginseng.Conclusion: The anti-fatigue activity and pharmacokinetic results showed that Raphani Semen may reduce the pharmacological actions of P. ginseng.展开更多
目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱...目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱定性采用飞行时间质谱,正负离子模式扫描。结果：快速鉴别出莱菔子中13种化学成分。结论：建立了一种简单可靠的HPLC-TOF/MS方法对莱菔子中主要化学成分进行了鉴定。展开更多
基金supported by Central Public-interest Scientific Institution Basal Research Fund (No. 1610342017017)
文摘Objective: To explain the phenomenon that Panax ginseng is not compatible with Raphani Semen based on pharmacodynamics and pharmacokinetics.Methods: The forced swimming time and biochemical parameters such as blood lactate(BLA), serum urea nitrogen(SUN), and hepatic glycogen(GLU) were determined for anti-fatigue experiment. The UPLCMS/MS was used to analyze the pharmacokinetics of Rg1, Re, Rb1, and Rd after orally administration of P. ginseng and P. ginseng combined with Raphani Semen to rats. Pharmacokinetic differences of four ginsenosides between single uses of P. ginseng and combined with Raphani Semen were investigated.Results: The results showed that Raphani Semen tended to significantly reduce the anti-fatigue activity of P. ginseng. Co-administration of P. ginseng and Raphani Semen had significant effects on the pharmacokinetics of the four ginsenosides in rats compared to that observed with P. ginseng extract alone. The AUC0–12 hvalues of the four ginsenosides in PG group were higher than the corresponding values in the PR group. It can be inferred that Raphani Semen decreased the blood exposure of the four ginsenosides in rats when it combined with P. ginseng.Conclusion: The anti-fatigue activity and pharmacokinetic results showed that Raphani Semen may reduce the pharmacological actions of P. ginseng.
文摘目的：通过高效液相色谱-飞行时间质谱（HPLC-TOF/MS）联用技术定性分析莱菔子中的主要化学成分。方法：色谱分离采用Diamonsil-C18柱（2.1 mm×150 mm,3μm）;流动相组成为乙腈-水（含0.1%醋酸）,梯度洗脱,流速为0.3 m L/min,质谱定性采用飞行时间质谱,正负离子模式扫描。结果：快速鉴别出莱菔子中13种化学成分。结论：建立了一种简单可靠的HPLC-TOF/MS方法对莱菔子中主要化学成分进行了鉴定。
