We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/...We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.3.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.3.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.展开更多
Co-seismic strain changes of the Wenchuan MwT. 9 earthquake recorded with three four-component borehole strainmeters showed NW-SE and roughly EW extensions, respectively, at two locations in the interior and northern ...Co-seismic strain changes of the Wenchuan MwT. 9 earthquake recorded with three four-component borehole strainmeters showed NW-SE and roughly EW extensions, respectively, at two locations in the interior and northern part of Tibetan plateau, and NS shortening at a location south of the epicenter, in agreement with the tectonic stress field of this region retical values obtained with half-space effects, such as local crustal structure The observed values of as much as 10-7 are, however, larger than theo- and spherical-earth dislocation theories, implying the existence of other and initial stress.展开更多
[ Objective ] The paper was to prevent the occurrence of broiler avian influenza virus HS subtype Re-8 strain effectively in the breeding process of broilers. [Method] The maternal antibodies of broilers in Beijing Ba...[ Objective ] The paper was to prevent the occurrence of broiler avian influenza virus HS subtype Re-8 strain effectively in the breeding process of broilers. [Method] The maternal antibodies of broilers in Beijing Baochen Hongwang farm were monitored. According to the disappearance law of maternal antibody, the optimal immune time of broiler avian influenza virus H5 subtype Re-8 strain was determined. [ Result] The maternal antibody level of 2-day-old broilers was relatively high, concentrated at 6 log2 -9 log2, and the antibody positive rate was 100%. The maternal antibody level of 8-day-old broilers concentrated at 4 log2 -6 log2, and the antibody positive rate was 100%. The maternal antibody level of 17-day-old broilers concentrated at 0 log2 -3 log2 , and the antibody positive rate was 0. The average maternal antibody level of 24 - 37 days old broilers was 〈 1 log2, and the antibody positive rate was 0. [ Conclusion ] Although the av- erage maternal antibody level of 8-day-old broilers was higher than 5 log2 , 20% of chickens was 4 log2, and maternal antibody could not protect the flock completely. Therefore, the best primary immunization day age of chicks against avian influenza virus was 8 - 10 days of age.展开更多
通过硫酸铵分级沉淀、DEAE Sepharose Fast Flow柱层析、Sephadex G-200柱层析,以小麦蠕孢病菌和金黄色葡萄球菌为指示菌采用抑菌活性追踪和SDS-PAGE跟踪检测,从分离自连云港海域、对多种病原真菌具有抑菌作用的多黏类芽孢杆菌L1-9菌株...通过硫酸铵分级沉淀、DEAE Sepharose Fast Flow柱层析、Sephadex G-200柱层析,以小麦蠕孢病菌和金黄色葡萄球菌为指示菌采用抑菌活性追踪和SDS-PAGE跟踪检测,从分离自连云港海域、对多种病原真菌具有抑菌作用的多黏类芽孢杆菌L1-9菌株发酵液中分离纯化得到一种对金黄色葡萄球菌和小麦蠕孢病菌的菌丝生长及孢子萌发具有抑制作用的抗菌蛋白,分子质量约31kD。展开更多
The Kunlunshan Mountain Ms8.1 earthquake, occurred in Nov.14, 2001, is the first event with magnitude more than 8 in the China earthquake monitoring history, specifically at the beginning of digital techniques in prec...The Kunlunshan Mountain Ms8.1 earthquake, occurred in Nov.14, 2001, is the first event with magnitude more than 8 in the China earthquake monitoring history, specifically at the beginning of digital techniques in precursor monitoring networks. Any investigation of recorded data on this earthquake is very important for testing the operation of the digital monitoring networks and understanding the preparation, occurrence, and adjustment of stress/strain of strong continental earthquakes. In this paper we investigated the coseismic response changes of well water level of groundwater and volume strain meter of bore hole in digital earthquake monitoring network of Capital area and its vicinity, due to the Nov.14, 2001 Ms8.1 Kunlun Mountain earthquake. The responding time, shapes or manners, amplitudes, and lasting time of well water level and strain-meters to seismic wave are studied in comparison. Then we discussed the possibility that the response changes of groundwater to strong distant earthquakes can be understood as one kind of observing evidence of stress/strain changes induced by distant earthquake.展开更多
基金This work was supported by the National Key R&D Program of China(2016YFD0501602,2017YFD0500701,and 2016YFEO203200)the National Natural Science Foundation of China(3167131307)+1 种基金the China Agriculture Research System(CARS-41-G12)and Central Publicinterest Scientific Institution Basal Research Fund(1610302017001).
文摘We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.3.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.3.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.
基金supported by Basic Research Foundation from Institute of Earthquake Science (0210240101)Seismological Science Foundation for Young Scientist of Earthquake Administration of Gansu Province and Lanzhou Institute of Seismology,CEA
文摘Co-seismic strain changes of the Wenchuan MwT. 9 earthquake recorded with three four-component borehole strainmeters showed NW-SE and roughly EW extensions, respectively, at two locations in the interior and northern part of Tibetan plateau, and NS shortening at a location south of the epicenter, in agreement with the tectonic stress field of this region retical values obtained with half-space effects, such as local crustal structure The observed values of as much as 10-7 are, however, larger than theo- and spherical-earth dislocation theories, implying the existence of other and initial stress.
文摘[ Objective ] The paper was to prevent the occurrence of broiler avian influenza virus HS subtype Re-8 strain effectively in the breeding process of broilers. [Method] The maternal antibodies of broilers in Beijing Baochen Hongwang farm were monitored. According to the disappearance law of maternal antibody, the optimal immune time of broiler avian influenza virus H5 subtype Re-8 strain was determined. [ Result] The maternal antibody level of 2-day-old broilers was relatively high, concentrated at 6 log2 -9 log2, and the antibody positive rate was 100%. The maternal antibody level of 8-day-old broilers concentrated at 4 log2 -6 log2, and the antibody positive rate was 100%. The maternal antibody level of 17-day-old broilers concentrated at 0 log2 -3 log2 , and the antibody positive rate was 0. The average maternal antibody level of 24 - 37 days old broilers was 〈 1 log2, and the antibody positive rate was 0. [ Conclusion ] Although the av- erage maternal antibody level of 8-day-old broilers was higher than 5 log2 , 20% of chickens was 4 log2, and maternal antibody could not protect the flock completely. Therefore, the best primary immunization day age of chicks against avian influenza virus was 8 - 10 days of age.
文摘通过硫酸铵分级沉淀、DEAE Sepharose Fast Flow柱层析、Sephadex G-200柱层析,以小麦蠕孢病菌和金黄色葡萄球菌为指示菌采用抑菌活性追踪和SDS-PAGE跟踪检测,从分离自连云港海域、对多种病原真菌具有抑菌作用的多黏类芽孢杆菌L1-9菌株发酵液中分离纯化得到一种对金黄色葡萄球菌和小麦蠕孢病菌的菌丝生长及孢子萌发具有抑制作用的抗菌蛋白,分子质量约31kD。
基金supported by Natural Science Foundation of China(41274061 and 40374019)
文摘The Kunlunshan Mountain Ms8.1 earthquake, occurred in Nov.14, 2001, is the first event with magnitude more than 8 in the China earthquake monitoring history, specifically at the beginning of digital techniques in precursor monitoring networks. Any investigation of recorded data on this earthquake is very important for testing the operation of the digital monitoring networks and understanding the preparation, occurrence, and adjustment of stress/strain of strong continental earthquakes. In this paper we investigated the coseismic response changes of well water level of groundwater and volume strain meter of bore hole in digital earthquake monitoring network of Capital area and its vicinity, due to the Nov.14, 2001 Ms8.1 Kunlun Mountain earthquake. The responding time, shapes or manners, amplitudes, and lasting time of well water level and strain-meters to seismic wave are studied in comparison. Then we discussed the possibility that the response changes of groundwater to strong distant earthquakes can be understood as one kind of observing evidence of stress/strain changes induced by distant earthquake.
