Premature aging of skeletal stem/progenitor cells rather than osteoblasts causes bone loss with decreased mechanosensation

A distinct population of skeletal stem/progenitor cells(SSPCs)has been identified that is indispensable for the maintenance and remodeling of the adult skeleton.However,the cell types that are responsible for age-related bone loss and the characteristic changes in these cells during aging remain to be determined.Here,we established models of premature aging by conditional depletion of Zmpste24(Z24)in mice and found that Prx1-dependent Z24 deletion,but not Osx-dependent Z24 deletion,caused significant bone loss.However,Acan-associated Z24 depletion caused only trabecular bone loss.Single-cell RNA sequencing(sc RNA-seq)revealed that two populations of SSPCs,one that differentiates into trabecular bone cells and another that differentiates into cortical bone cells,were significantly decreased in Prx1-Cre;Z24^(f/f)mice.Both premature SSPC populations exhibited apoptotic signaling pathway activation and decreased mechanosensation.Physical exercise reversed the effects of Z24depletion on cellular apoptosis,extracellular matrix expression and bone mass.This study identified two populations of SSPCs that are responsible for premature aging-related bone loss.The impairment of mechanosensation in Z24-deficient SSPCs provides new insight into how physical exercise can be used to prevent bone aging.

Electrophysiological evaluation of alterations in penile sensation due to penile prosthesis implantation in patients with erectile dysfunction

Objective The present study was aimed to determine the effect of penile prosthesis implantation(PPI)surgery on penile sensation by evaluating the penile electromyography(EMG)variables.Methods The research was designed as a prospective study.Thirty patients who were diagnosed with organic erectile dysfunction and had underwent PPI surgery between January 2017 and January 2018 in the Urology Clinic of Antalya Training and Research Hospital were included in this study.Penile sensory EMG was performed on each patient 1 day prior to the surgery by the Physical Therapy and Rehabilitation clinic.Additionally,the control EMG study was also performed in the 3rd and 6th postoperative months.Results We included 27 patients in this study who attended regular follow-ups and had complete EMG results.Out of the 27 patients,23(85.2%)patients had received malleable(ProMedon)penile prosthesis,one(3.7%)patient had received a two-piece inflatable(Ambicor)penile prosthesis,and three(11.1%)patients had received a three-piece inflatable(AMS 700 CXR)penile prosthesis.The mean nerve conduction value(NCV)of the patients was 29.85(standard derivation:22.54;range:0-78.4)m/s during the preoperative period,whereas it was 27.64(standard derivation:24.72;range:0-83.3)m/s in the 3rd postoperative month and 24.80(standard derivation:22.31;range:0-88.4)m/s in the 6th postoperative month.There was no significant difference between preoperative NCV and 3rd postoperative month NCV(p=0.607).Similarly,no significant difference was observed between preoperative NCV and 6th postoperative month NCV(p=0.276).Additionally,the change between NCV values at postoperative 3rd and 6th months was not statistically significant(p=0.553).Conclusion Significant loss of penile sensation does not occur in patients who undergo PPI surgery.

Effect of local thermal sensation on whole-body thermal sensation under stable non-uniform environment

To reveal the principles of human thermal responses and find out the effects of body parts on whole-body thermal sensation,through a subjective survey,experimental investigations on human responses are carried out when a single body part is thermally stimulated.Cooling airflow is sent to seven body parts,respectively.Totally 94 samples are tested.To eliminate the obvious multicollinearity of thermal sensation among different body parts,the principal component regression approach is adopted to obtain the principal components for the body parts under different experimental conditions.Through regression and analysis of principal components,the weighting factors of the seven body parts are obtained.A predictive model on whole-body thermal sensation is obtained based on the weighting factors.The results show that the different characteristics of trunk and limbs are clearly seen.The weighting factors of local thermal sensation are integrated values,and there is little difference among values of different body parts.

2例西门子Sensation16排CT故障维修

文本介绍了西门子Sensation16排CT主、副台变慢和相机通讯错误的解决办法。

Sensation 64 CT探测器隐性故障的检测及排除

文章分析了Sensation64CT机探测器故障的检测及维修软件的使用方法。介绍了利用软件分析手段诊断出探测器隐性故障并加以排除,保障了设备的正常使用的实例。文章最后提出了维修工程师的工作思路转变问题。

西门子SenSation64螺旋CT的保养与维修分析

近年来,我院引进了西门子SenSation64螺旋CT,该CT机具有极快的单源CT成像能力,适用于全身各部位的CT检查,可获得任意部位任意平面的高分辨率、高清晰度及高诊断细节的图像。从安装至现在,我院科室上下齐心协力,设备的保养与维修良好,取得较好的效益。针对该设备的维修与保养,本文从以下几个方面进行探讨和分析。

西门子Sensation 4型CT的故障及维修三例

CT在医院诊疗过程中发挥着重要作用,在其出现故障后应该围绕着故障现象,结合它的原理构造,认真分析排查,降低维修成本[1]。Sensation 4型CT在我院已使用近11年,现简要介绍3例故障和解决方法。1故障案例一故障现象：图像中间有环形伪影,影响图像质量。故障分析：引起环形伪影的原因有很多,如环境因素（温度、湿度、空气净化度）,过高或过低都会造成伪影[2];通道放大板故障;探测器之间响应不一致;AD控制板故障。

西门子Sensation64螺旋CT的维护与管理

我院于2009年6月引进西门子公司SOMATOM Sensation64螺旋CT设备,从安装使用至今,设备得以良好的运行。在工作中,我们认识到,对该CT设备进行日常质量控制和定期维护管理,是CT成像质量的保证,现介绍我们日常工作的几点做法,仅供参考。

Draft sensation distribution of air jet supply system in large space building in summer

To study the draft sensation distribution of an air jet supply system in a large space building in summer,experiments are conducted in a large laboratory.The temperature,velocity and draft sensation distributions at a nozzle height of 4 m in the occupied zone are obtained.Then,the numerical simulation under the test condition is carried out by the computational fluid dynamics（CFD）method.The calculation results of the indoor vertical temperature and the draft sensation distribution are validated by the test data.Simulations with different nozzle heights are conducted.The satisfactory air supply condition is determined by analyzing the draft sensations and the temperatures in the occupied zone under three conditions.The simulation results show that the optimal draft sensation distribution and the uniform temperature and velocity fields can be obtained at a nozzle height of 5 m.

西门子Sensation 64螺旋CT的实例故障分析

医院西门子Sensation 64层螺旋CT自08年投入使用以来运转正常,使用数年后故障频出,对其中两例典型故障及维修实践进行小结,供同行参考。 1故障案例一 （1）故障现象。机器正常扫描患者时中断,关机30 s后重新启动正常,发生频率每日2-3次,经关掉Caredose 4D后设备能正常工作。

Functional brain imaging of gastrointestinal sensation in health and disease

It has since long been known, from everyday experience as well as from animal and human studies, that psychological processes-both affective and cognitive- exert an influence on gastrointestinal sensorimotor function. More specifically, a link between psychological factors and visceral hypersensitivity has been suggested, mainly based on research in functional gastrointestinal disorder patients. However, until recently, the exact nature of this putative relationship remained unclear, mainly due to a lack of non-invasive methods to study the （neurobiological） mechanisms underlying this relationship in non-sleeping humans. As functional brain imaging, introduced in visceral sensory neuroscience some 10 years ago, does provide a method for in vivo study of brain-gut interactions, insight into the neurobiological mechanisms underlying visceral sensation in general and the influence of psychological factors more particularly, has rapidly grown. In this article, an overview of brain imaging evidence on gastrointestinal sensation will be given, with special emphasis on the brain mechanisms underlying the interaction between affective ＆ cognitive processes and visceral sensation. First, the reciprocal neural pathways between the brain and the gut （brain- gut axis） will be briefly outlined, including brain imaging evidence in healthy volunteers. Second, functional brain imaging studies assessing the influence of psychological factors on brain processing of visceral sensation in healthy humans will be discussed in more detail. Finally, brain imaging work investigating differences in brain responses to visceral distension between healthy volunteers and functional gastrointestinal disorder patients will be highlighted.

The existence of propagated sensation along the meridian proved by neuroelectrophysiology

Propagated sensation along the meridian can occur when acupoints are stimulated by acupuncture or electrical impulses. In this study, participants with notable propagated sensation along the me- ridian were given electro-acupuncture at the Jianyu （LI15） acupoint of the large intestine meridian. When participants stated that the sensation reached the back of their hand, reguJar nervous system action discharge was examined using a physiological recording electrode placed on the superficial branch of the radial nerve. The topographical maps of brain-evoked potential in the primary cortical somatosensory area were also detected. When Guangming （GB37） acupoint in the lower limb and Hegu （LI4） acupoint in the upper limb were stimulated, subjects without propagated sensation along the meridian exhibited a high potential reaction in the corresponding area of the brain cortical somatosensory area. For subjects with a notable propagated sensation along the meridian, the re- action area was larger and extended into the face representative area. These electrophysiological measures directly prove the existence of propagated sensation along the meridian, and the periph- eral stimulated site is consistent with the corresponding primary cortical somatosensory area, which presents a high potential reaction.

Is there an association between Helicobacter pylori in the inlet patch and globus sensation?

AIM:To determine the association between Helicobacter pylori(H.pylori)and globus sensation(GS)in the patients with cervical inlet patch. METHODS:Sixty-eight patients with esophageal inlet patches were identified from 6760 consecutive patients undergoing upper gastrointestinal endoscopy prospectively.In these 68 patients with cervical inlet patches, symptoms of globus sensation(lump in the throat), hoarseness,sore throat,frequent clearing of the throat,cough,dysphagia,odynophagia of at least 3 mo duration was questioned prior to endoscopy. RESULTS:Cervical heterotopic gastric mucosa(CHGM) was found in 68 of 6760 patients.The endoscopic prevalence of CHGM was determined to be 1%.H.pylori was identified in 16(23.5%)of 68 patients with inlet patch.53 patients were classified as CHGMⅡ.This group included 48 patients with globus sensation,4 patients with chronic cough and 1 patient with hoarseness.All the patients who were H.pylori(+)in cervical inlet patches had globus sensation.CONCLUSION:Often patients with CHGM have a long history of troublesome throat symptoms.We speculate that disturbances in globus sensation are like non-ulcer dyspepsia.

Formation mechanism of propagated sensation along the meridians,as verified by cortical somatosensory-evoked potential topographic maps

The peripheral driver stimulating theory states that as a driver passes along a certain meridian during acupuncture; the driver provokes nerve sense devices along the meridian, resulting in the nerve impulse entering the central nervous system. Accordingly, volunteers have reported propagated sensations along the meridians （PSM）. The present study was designed to utilize a cortical somatosensory-evoked potential （CSEP） topographic map for determining whether stimulation expansion occurs in somatosensory area I when sensation was provoked in individuals with obvious PSM. The sensation was blocked by mechanical compression, and the sensation was imitated in individuals without PSM. Results revealed a red, high-potential signal in the representative area of the lower limbs in individuals with obvious PSM symptoms when the Gall Bladder Meridian （GBM） sensation passed to the head and face. This representative area was near the middle line of the CSEP topographic map, and a red, high-potential signal, which jumps over the representative area of the upper limbs, also appeared in the representative face area, which was at the external region of the CSEP topographic map. However, in individuals exhibiting no PSM, only a red high-potential signal appeared in the representative lower limb area. When Hegu （LI 4） was stimulated in individuals without PSM, an obvious evoked response appeared only in the representative upper limb area. However, when Hegu was stimulated in individuals exhibiting PSM, the response area was larger in the representative upper limb area and extended to the representative face area. When Guangrning （GB 37） was stimulated in PSM individuals, the face representation response disappeared and was confined to a foot representation of the somatosensory area I when PSM was blocked by mechanical pressure. Results suggested that mechanical compression blocked PSM, and corresponding changes were exhibited in the CSEP topographic map. These results provide compelling evidence for the hypothesis that peripheral driver stimulation is the key element in producing PSM.

Study on the Relation between the Air Gap Size under Clothing and the Comfort Sensations

Combining two non-contact testing instruments of a three-dimensional body measure system and an infrared camera, the entrapped air gap under clothing and the clothing surface temperature distribution were measured without interfering the air gap state. It was discussed the effect of different air gap size on subjective clothing sensation. At the same time, taken the subjective sensations of moisture, adhesiveness and mugginess from the lower back as example, the relationship among the subjective evaluation, the air gap size and the clothing surface temperature were analyzed.

西门子sensation16 CT床水平运动故障一例

本文通过对西门子sensation系列CT机床水平运动故障一例的分析,了解床水平运动的机构的组成和原理。

Rikkunshito improves globus sensation in patients with proton-pump inhibitor-refractory laryngopharyngeal reflux

AIM: To investigate the effect of rikkunshito on laryngopharyngeal reflux(LPR) symptoms and gastric emptying in patients with proton-pump inhibitor(PPI)refractory LPR.METHODS: In total,22 patients with LPR were enrolled.Following a 2-wk treatment with PPI monotherapy,PPI-refractory LPR patients were randomly divided into two treatment groups(rikkunshito alone or rikkunshito plus the PPI,lansoprazole).LPR symptoms were assessed using a visual analog scale(VAS) score,gastrointestinal symptoms were assessed using the gastrointestinal symptom rating scale(GSRS),and gastric emptying was assessed using the radio-opaque marker method prior to and 4 wk following treatments.RESULTS: The 4-wk treatment with rikkunshito alone and with rikkunshito plus the PPI significantly decreased the globus sensation VAS scores.The VAS score for sore throat was significantly decreased following treatment with rikkunshito plus PPI but not by rik kunshito alone.Neither treatment significantly changed the GSRS scores.Rikkunshito improved delayed gastric emptying.We found a significant positive correlation between improvements in globus sensation and in gastric emptying(r2= 0.4582,P < 0.05).CONCLUSION: Rikkunshito improved globus sensation in patients with PPI-refractory LPR,in part,because of stimulation of gastric emptying.Thus,rikkunshito is an effective treatment for PPI-refractory LPR.

EFFECT OF NEEDLING SENSATION REACHING THE SITE OF DISEASE ON THE RESULTS OF ACUPUNCTURE TREATMENT OF BRONCHIAL ASTHMA

Effective treatment of bronchial asthmawith various methods of acupuncture has beenreported in the literature.The present articlediscusses the results of the treatment of bron-chial asthma(BA)and chronic senile bronchitis(CSB)according to the phenomenon of needlingsensation reaching the site of disease during acu-puncture.

Effects of electroacupuncture on pain sensation in a rat model of hyperalgesia with nicotine dependence

Tobacco smoking is considered to be one of the main risk factors in the development of chronic pain.Long-term chronic exposure to nicotine and other forms of tobacco have been shown to be associated with an increased incidence of pain.Studies have shown that acupuncture can help smokers to reduce their desire to smoke,reduce their withdrawal symptoms,and avoid a relapse after treatment.However,little has been reported about the effects of acupuncture on pain sensitivity caused by long-term smoking.Models of hyperalgesia were established in rats exposed to nicotine for 6 weeks.After 6 weeks of continuous nicotine exposure,electroacupuncture at bilateral acupoints Zusanli(ST36)and Taichong(LR3)was performed 20 minutes per day for 6 days at a continuous wave with a frequency of 2 Hz and a stimulus intensity of 1 m A.The results revealed that electroacupuncture treatment increased the mechanical response threshold of hind paw of nicotine-dependent rats with hyperalgesia and up-regulated the protein expression of pain-related factorsμ-opioid receptor,β-endorphin and glutamic acid decarboxylase 65 in the spinal cord and midbrain periaqueductal gray and the protein expression of glutamic acid decarboxylase 67 in the spinal cord.These findings suggest that electroacupuncture treatment has positive analgesic effects on pain sensitivity caused by long-term chronic nicotine exposure.One possible mechanism for the improved analgesia is that electroacupuncture increases the expression of painrelated factors in the spinal cord and midbrain periaqueductal gray.This study was approved by Institutional Animal Care and Use Committee(IACUC)of the University of Miami(#18-167)on December 12,2018.

Animal models and experimental medicine and the Nobel Prize in Physiology or Medicine 2021-TRPV and PIEZO receptors for temperature and touch sensation

1|INTRODUCTION For decades,experimental animal models have been powerful tools for biomedical research and have supported most of the physiological or medical achievements recognized by Nobel Prizes,including the research that won this year's Physiology or Medicine Prize.On 4th October 2021,the Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian"for their discoveries of receptors for temperature and touch."1 Their discoveries have profoundly changed our view of how we sense the world around us2.