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The Transcription Factors GATA-1 and GATA-4 Have Opposite Effects on DNA Expression Driven by an Amh Promoter
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作者 David W. Dresser 《American Journal of Molecular Biology》 2014年第3期150-158,共9页
An Amh promoter driving expression of a reporter gene (d2EGFP) has been used to analyze the role of two specific promoter transcription factor binding elements. In addition a downstream (3’) enhancer (DE) was also in... An Amh promoter driving expression of a reporter gene (d2EGFP) has been used to analyze the role of two specific promoter transcription factor binding elements. In addition a downstream (3’) enhancer (DE) was also investigated. The transcription factors GATA-1 and GATA-4 had opposite effects, the former being incremental and the latter decremental. The quantitative balance between these two factors may provide a degree of control over the level of gene expression. 展开更多
关键词 smat-1 Mouse Pre-Pubertal SERTOLI In Vitro AMH PROMOTER d2EGFP Transcription Factors GATA-1 GATA-4
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Mutated elements of a complex promoter (Amh) can help to demonstrate the role of certain elements in controlling differential gene expression 被引量:1
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作者 David W. Dresser 《American Journal of Molecular Biology》 2012年第4期351-358,共8页
Amh is a single copy gene which is expressed in different ways during mammalian development. Several potential promoter elements have been identified using physiological experimentation and on the basis of interspecif... Amh is a single copy gene which is expressed in different ways during mammalian development. Several potential promoter elements have been identified using physiological experimentation and on the basis of interspecific sequence comparison. The role of putative promoter elements in controlling gene expression has been investigated by many workers over the last two decades and here by individually mutating each element. Expression was measured in vitro in cells of Sertoli descent by flowcytometry using EGFP as a reporter gene. Three lines of murine cells were used;pre- and post-pubertal Sertoli and granulosa cells. Differences between the three lines of cells, support the view that differentiation in this in vitro model system is likely to be at the level of available transcription factors at given points in development. 展开更多
关键词 Anti-Mullerian HORMONE SDM Site Directed Mutation Murine smat-1 TM4 SERTOLI CELL Lines KK1 GRANULOSA CELL Line
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