Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and inflammation.AIM To investigate the association between ferroptosis and pyroptosis and the upstream regulatory mechanisms.METHODS This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce ALF.Gasdermin D(GSDMD)^(-/-)mice were used as an experimental group.Histological changes in liver tissue were monitored by hematoxylin and eosin staining.ALT,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial kits.Ferroptosis-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain reaction.SIRT1,p53,and GSDMD were assessed by immunofluorescence analysis.RESULTS Serum AST and ALT levels were elevated in patients with ALF.SIRT1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver tissue.In the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was mitigated.In mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were detected.In vitro,knockdown of p53 and overexpression of GPX4 reduced AST and ALT levels,the cytostatic rate,and GSDMD expression,restoring SLC7A11 depletion.Moreover,SIRT1 agonist and overexpression of SIRT1 alleviated acute liver injury and decreased iron deposition compared with results in the model group,accompanied by reduced p53,GSDMD,and ACSL4,and increased SLC7A11 and GPX4.Inactivation of SIRT1 exacerbated ferroptotic and pyroptotic cell death and aggravated liver injury in LPS/D-GalNinduced in vitro and in vivo models.CONCLUSION SIRT1 activation attenuates LPS/D-GalN-induced ferroptosis and pyroptosis by inhibiting the p53/GPX4/GSDMD signaling pathway in ALF.

Cracking the silent gallstone code:Wait or operate?

The widespread availability of abdominal ultrasound has revealed the common occurrence of asymptomatic gallstones.While the treatment for symptomatic gallstones is clear,the benefits of minimally invasive laparoscopic cholecystectomy have sparked debate about the best approach to managing silent gallstones.The potential for asymptomatic gallstones to become symptomatic or lead to complications complicates the decision-making process regarding surgical intervention,as it's uncertain when or which patients might develop complications.Consequently,risk stratification appears to play a critical role in guiding decisions about silent gallstones.However,there is no definitive evidence to direct management,and a consensus-based on high-quality evidence is yet to be established.

The Silent Curriculum:Classical Aesthetics as a Catalyst for Emotional and Social Skills in Higher Vocational Education

This study examines the integration of classical aesthetics into the silent curriculum of higher vocational education,emphasizing its potential to significantly enhance emotional and social skills.Incorporating aesthetic principles into education emphasizes the importance of nurturing emotional intelligence,creativity,and cultural awareness in students-skills that go beyond the classroom and are essential for their growth,adaptability,and future careers.It explores theoretical foundations and practical implementations and addresses challenges such as the need for specialized educator training,overcoming institutional resistance,and securing adequate resources.Advocating for empirical research and strategic cultural partnerships,the paper proposes a transformative approach to vocational education,aligning it with contemporary societal and labor market demands,and underscores the vital role of classical aesthetics in enriching vocational training and enhancing student outcomes.

少年儿童翻译文学的译本风格计量研究——以Silent Spring三译本为例

本研究基于自建Silent Spring(《寂静的春天》)英汉平行语料库,以教育部编义务教育语文教材中的科普文章为标准参照,从可读性、趣味性和具象性三个维度,探讨少年儿童文学翻译文本风格特征及其与目标读者认知水平和接受能力的契合度,希冀能拓宽少年儿童翻译文学风格计量研究视野,为少年儿童文学的翻译传播研究提供参考。

高校英语专业综合英语课程思政的教学设计研究——以“Silent Spring”一课的教学为例

课程思政的全面实施是新时代中国教育落实立德树人根本任务的迫切需求。综合英语课程作为高校英语专业的一门基础必修课,承担着重要的育人任务。在重新审视教学大纲和教学内容的前提下,本文对《现代大学英语精读3》(第二版)教材中“Silent Spring”一文思政元素融入的教学设计进行探讨和反思,以期在方法和模式上为相关课程实施课程思政提供启示和借鉴。

Protective Effect of Silent Mating Type Information Regulation 2 Homolog 1 on TGF-β1 Pathway via mTOR in Diabetic Nephropathy

Objective: To demonstrate whether the expression of silent mating type information regulation 2 homolog 1 (SIRT1) affects the level of TGF-β1 and Smad3 in HEK293 cells through regulating mTOR. Methods: First, recombinant plasmids DNA (rSIRT1) and siRNA targeting SIRT1 were constructed which were transfected into Human Embryonic Kidney 293 cell (HEK293) cells, respectively. Then, the generation of intracellular ROS in cells was examined by flow cytometry using the oxidation-sensitive probe. Last, the expressions of TGF-β1, smad3, P53, mTOR, p-mTOR, LC3-I and LC3-II in cells were detected to observe the effect of SIRT1 on TGF-β1 Pathway by western blot analysis. Results: We demonstrated that overexpressing of SIRT1 may decrease TGF-β1 and Smad3 expression in HEK293 cells through regulating mTOR. In addition, the result is the opposite when SIRT1 was silent in HEK293 cells. Conclusions: SIRT1 is closely related to TGF-β1/Smad3 pathway that correlates with the regulation of mTOR and ROS generation and causes diabetic nephropathy. The available evidence implies that SIRT1 has great potential as a clinical target for the prevention and treatment of renal fibrosis in the development of DN.

基于DIgSILENT数据接口DGS的含风电电力系统随机潮流分析

同一风区的不同风电场由于地理位置相近,各风电场的风速和出力具有强相关性。针对该特点,提出了一种考虑风电场之间相关性的含风电电力系统随机潮流算法。首先建立了多个风电场出力的离散化联合概率分布模型,在此基础上采用线性化潮流方法分析随机潮流的各项指标。结合随机潮流算法和DIg SILENT软件的优势,研究了一种基于DIg SILENT数据接口DGS的数据转换方法,有效地提高了计算效率。最后利用IEEE-24节点系统以及我国新疆地区2015年规划电网进行方法验证。算例结果表明,建立的考虑风电场相关性的含风电电力系统随机潮流算法具有可行性和优越性,DGS数据接口技术可以使该潮流算法在实际大型电力系统中推广使用,工程实用性较强。

PROPER CONDITIONS OF MESHING FOR Hy-Vo SILENT CHAIN AND SPROCKET

Proper meshing of Hy-Vo silent chain and sprocket is important for realizing the transmission of the silent chain with more efficiency and less noise. Based on the study of the meshing theory of the Hy-Vo silent chain with the sprocket and the roll cutting machining principle of the sprocket with the hob, the proper conditions of the meshing for the Hy-Vo silent chain and the sprocket are put forward with the variable pitch characteristic of the Hy-Vo silent chain taken into consideration, and the proper meshing design method on the condition that the value of the link tooth pressure angle is unequal to the value of the sprocket tooth pressure angle is studied. Experiments show that this new design method is feasible. In addition, the design of the pitch, the sprocket tooth pressure angle and the fillet radius of the sprocket addendum circle are studied. It is crucial for guiding the design of the hob which cuts the Hy-Vo silent chain sprocket.

E文本在线电网模型自动导入DIgSILENT的研究和开发

为了将DIgSILENT/Power Factory集成为调度运行和规划分析高层应用平台中的核心计算工具,研究在线电网模型自动导入和计算结果自动导出DIgSILENT的技术。采用IEEE9节点电网,研究电网设备模型、拓扑模型和图形模型在DGS接口文件中的数据内容和格式,测试验证利用DGS接口导入全网模型的可行性。对E文本在线电网模型和DGS接口电网模型进行对比分析,研究一种基于电网拓扑数据自动生成DGS格式的电网图形数据的方法,设计E文本在线电网模型转为DGS接口电网模型的实现流程。最后开发相应的软件,并测试软件功能,同时分析总结潮流计算中设置节点类型的调试经验。研究内容和成果对于将DIgSILENT推广应用于在线运行分析和离线快速建模具有指导作用。

MESHING THEORY AND DESIGN METHOD OF NEW SILENT CHAIN AND SPROCKET

Based on the study of the meshing theory of a new silent chain and sprockets, and the rolling cutting theory of sprocket and hob, the harmonious relations of dominating dimensions among the new silent chain, sprocket and hob is build, the meshing conditions are expatiated, and the resolved expression, which can instruct design and calculation, is educed. The tests show that the meshing design method is feasible.

Degradation of Benzene by Using a Silent-Packed Bed Hybrid Discharge Plasma Reactor

In this work, a novel gas phase silent-packed bed hybrid discharge plasma reactor has been proposed, and its ability to control a simulative gas stream containing 240 ppm benzene is experimentally investigated. In order to optimize the geometry of the reactor, the benzene conversion rate and energy yield （EY） were compared for various inner electrode diameters and quartz tube shapes and sizes. In addition, benzene removal efficiency in different discharge regions was qualitatively analyzed and the gas parameter （space velocity） was systematically studied. It has been found that silent-packed bed hybrid discharge plasma reactor can effectively decompose benzene. Benzene removal proved to achieve an optimum value of 60% with a characteristic energy density of 255 J/L in this paper with a 6 mm bolt high-voltage electrode and a 13 mm quartz tube. The optimal space velocity was 188.1 h^-1, which resulted in moderate energy yield and removal efficiency. Reaction by-products such as hydroquinone, heptanoic acid, 4-nitrocatechol, phenol and 4-phenoxy-phenol were identified by mean of GC-MS. In addition, based on these organic by-products, a benzene destruction pathway was proposed.

miR-211 promotes lens epithelial cells apoptosis by targeting silent mating-type information regulation 2 homolog 1 in age-related cataracts

AIM： To detect the expression of miR-211 in age-related cataract tissue, explore the effects of miR-211 on lens epithelial cell proliferation and apoptosis, and identify its target gene.METHODS： This study used real-time quantitative polymerase chain reaction（RT-q PCR） to measure the expression of miR-211 and its predicted target gene [silent matingtype information regulation 2 homolog 1（SIRT1）] in 46 anterior lens capsules collected from age-related cataract patients. Human lens epithelial cell line（SRA01/04） cells were transfected with either miR-211 mimics, mimic controls, miR-211 inhibitors or inhibitor controls, 72 h after transfection, miR NA and protein expression of SIRT1 were measured using RT-qP CR and Western blotting; then cells were exposed to 200 μmol/L H2O2 for 1h, whereupon cell viability was measured by MTS assay, caspase-3 assay was performed. Dual luciferase reporter assay was performed to verify the relationship between miR-211 of SIRT1.RESULTS： Compared to the control group, expression of miR-211 was significantly increased（P〈0.001）, the miR NA and protein expression of SIRT1 were significantly decreased（P〈0.001） in the anterior lens capsules of patients with age-related cataracts. Relative to the control group, SIRT1 miR NA and protein levels in the miR-211 mimic group were significantly reduced, cell proliferation activity significantly decreased, and caspase-3 activity was significantly increased（P〈0.001）. In the miR-211 inhibitor group, SIRT1 miRNA and protein expression were significantly increased, cell proliferation activity significantly increased, and caspase-3 activity was significantly decreased（P〈0.001）. A dual luciferase reporter assay confirmed that SIRT1 is a direct target of miR-211.CONCLUSION： miR-211 is highly expressed in the anterior lens capsules of patients with age-related cataracts. By negatively regulating the expression of SIRT1, miR-211 promotes lens epithelial cell apoptosis and inhibits lens epithelial cell proliferation.

Silent brain infarctions and leuko-araiosis in Chinese patients with first-ever acute lacunar strokes

We report on silent brain infarction (SBI) and leuko- araiosis (LA) of 23 patients with clinically diagnosed “first-ever” acute ischemic lacunar stroke. The lacunar syndromes were pure motor hemiparesis (10), pure sensory syndrome (2), ataxic hemiparesis (3), dysarthria clumsy hand syndrome (3), and sensory- motor deficit (5). Nineteen out of the 23 patients presented with completed strokes on arrival to the hospital, and 4 (17%) developed evolving-stroke within 24 hours of stroke onset. A lacune corresponded to the acute stroke could be found in all patients on brain magnetic resonance imaging (MRI), and in 18 (78%) on brain computed tomography (CT). MRI showed additional subclinical or asymptomatic “silent brain infarctions or lacunes” (SBI) in 19 (83%) of 23 patients, and leuko-araiosis (LA) of moderate to severe degree (> grade 2) was present in 61% of patients although dementia was absent. Hypertension is the risk factor in 78% of cases followed by diabetes mellitus, smoking, and elevated plasma cholesterol level. Independence of the types of lacunar syndromes, patients with hypertension and diabetes mellitus are associated with high grade LA. None with normal blood pressure and plasma glucose had grade 3 or grade 4 LA (p < 0.05). In conclusion, evolving-stroke occurs in one- fifth of patients with “first-ever” lacunar infarct within the first 24 hours of stroke onset. SBI was found in 83% of cases. Hypertension and diabetes mellitus are associated with additional SBI and high grade LA. The severity of leuko-araiosis per se dictates the cerebrovascular risks.

Silent diabetic nephropathy

AIM： To examine the risk of renal events in patients with biopsy-proven diabetic nephropathy （DN） and its possible associated factors.METHODS： Clinical and histological data of 60 pa-tients diagnosed with diabetic nephropathy were retro-spectively collected. Patients with evidence or suspicion of other nephropathies were excluded from the study. The fnal event was defned as renal replacement ther-apy （RRT） initiation or progression of chronic kidney disease （CKD）, according to the KDIGO 2012 defnition of a decrease in CKD category and a decrease in GFR of 25% or more. RESULTS： A total of 45 patients with a follow-up of at least 3 mo were included. Most of the patients presented type 2 DM, with a mean age of 58.3 years old. The time of evolution of DM was 9.6 ± 7.8 years, al-though in 13 patients, it was less than 5 years. A total of 62% of patients reached the fnal event in a mean period of 3.4 years （95%CI： 2.1-4.7）, with 21 of them requiring dialysis. The factors that were indepen-dently associated with renal survival were estimated glomerular fltration rate （eGFR） at the time of biopsy, cardiovascular disease （CVD） history and HbA1c less than 7%. Therefore, for each 10 mL/min per 1.73 m2 reduction in eGFR, we obtained a DN progression risk of HR = 2 （1.3-3.0） （P = 0.001）; patients with CVD were at greater risk for DN progression （HR = 2.8, 1.1-7.1, P = 0.032）, and CKD patients with HbA1c 〈 7% demonstrated greater renal risk than patients with HbA1c ≥ 7%, with an HR of 2.9 （1.0-8.4） （P = 0.054）.CONCLUSION： A past history of CVD is a risk fac-tor for DN progression. Levels of HbA1c less than 7% could favor an eGFR decrease in these patients.

An Extraordinary Case of Silent Extensive Anterior Wall Myocardial Infarction Complicated with Giant Left Ventricular Aneurysm and Dressler Syndrome

Early post-acute myocardial infarction (AMI) pericarditis, pericardial effusion with or without cardiac tamponade, and late post-MI pericarditis (Dressler syndrome), are the major pericardial complications after AMI. It is quite rare and estimated to be only about 0.1% in AMI patients according to a recent report, so it is easily neglected or misdiagnosed and may have tragic result to patient. Clinical features of this post-AMI complication include fever, chest pain, pericarditis and pleurisy occurring 2 to 3 weeks after AMI. Dressler syndrome is rarely associated with left ventricular aneurysm. Contrast enhanced magnetic resonance and echocardiography play important roles in diagnosis of left ventricle aneurysm. We report a 54-year-old male heavy labor worker who had asymptomatic, severe coronary artery disease, complicated with silent myocardial infarction, which resulted in large left ventricular aneurysm, and also systolic heart failure was noted. Patient was diagnosed to have Dressler syndrome after his second cardiology clinic follow-up. He received coronary angiography which revealed triple vessel disease with total occlusion of left anterior descending artery, and a giant left ventricular aneurysm was found. He received surgical intervention with Batista method and followed-up uneventfully at the cardiology clinic.

从功能派目的论看文学翻译——浅析“Silent Meaning”一诗之译文

自20世纪70年代产生至今,德国的功能派目的论对翻译实践活动的开展发挥了巨大的指导作用。在诺德的《译有所为——功能翻译理论阐释》一书中,我们可以了解到除非文学领域外,功能理论在文学翻译中也有较强的适用性,能够为文学翻译建立理论基础,使译者有章可循。本文试以"Silent Meaning"一诗针对不同读者群体的两种译本为例,以期向读者说明功能派目的论在文学翻译中的指导作用。

An Eternal Quest for Poetic Dwelling: A Comparative Study of the Eco-Ethic Thoughts in Silent Spring and Wolf Totem

This thesis is aimed at conducting a comparative study of the ecoethic thoughts in Silent Spring and Wolf Totem. It points out that Carson and Jiang's eco-ethics are relatively complete, ideal and objective. Moreover, the most shining features are that they not only unfold the grim fact of ecological crisis before the public, but also instil confidence and hope in each and every reader.

Application of Silent Way in Foreign Language Teaching

The Silent Way (SW) is language teaching method which teacher keeps silent much of the time making students to produce the new language. This paper aims to apply SW to English teaching and figure out the idea why Silent Way couldn't be a widely-adopted teaching method. In terms of the characteristics of this innovational teaching method, it has some advantages as well as inevitable defects.

指向深度学习的《综合英语》课程思政的教学设计——以寂静的春天(Silent Spring)一文的讲授为例

课程思政的全面实施是新时代中国教育落实立德树人根本任务的迫切需求。《综合英语》课程作为高校英语专业的一门基础必修课,承担着重要的育人任务。在重新审视教学大纲和教学内容的前提下,本文基于深度学习理论,对《现代大学英语精读3》(第二版)教材中“Silent Spring”(寂静的春天)一文思政元素融入的教学设计进行探讨和反思,以期在方法和模式上为相关课程实施课程思政提供启示和借鉴。