为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的...为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的体重、体高、体厚、眼径、体长和背鳍前长6个生长性状进行QTL定位分析。结果显示,多鳞[鱼喜]首张高密度遗传连锁图谱全长2 154.803 c M,标记间平均遗传距离0.455 c M,共有4 735个SNP标记分配到24个连锁群。QTL定位分析结果发现在6个生长性状中共检测到20个生长显著相关QTL位点,分布在8个连锁群上,单个QTL的LOD值范围为3.02~4.23,可解释的表型变异范围为0.14%~8.42%。其中,在连锁群LG08聚集了8个生长性状显著相关的QTL。通过对候选QTL区间内的基因进行功能注释,共筛选到了19个潜在生长调控相关基因,包含igf1、igf2、sstr5、sst1a、tgfbr2、gas1、igfals、gfg6、gfg20、bmp7、kdm5c、tti1以及rbm10等。实验获得的遗传标记及相关候选基因是多鳞[鱼喜]生长相关性状标记辅助选择(MAS)的有用基因资源,为进一步研究鱼类生长调控机制提供了更多的理论依据。展开更多
Otolith shape is species-specific in fish.In this study,161 samples of Trumpeter sillago Sillago maculata and 164 of Silver sillago Sillago sihama were collected from Beibu Gulf in July 2009.The main objective of this...Otolith shape is species-specific in fish.In this study,161 samples of Trumpeter sillago Sillago maculata and 164 of Silver sillago Sillago sihama were collected from Beibu Gulf in July 2009.The main objective of this study was to use morphological variables and Fourier harmonics to describe otolith characteristics and use discriminant analyses to separate the two species.Otoliths were measured by traditional one and two dimensional measures(otolith length,width,diameters,area,perimeter,rectangularity and circularity),as well as by Fourier analysis to capture the finer regions of the otolith.Analysis of covariance(ANCOVA) showed that there was significant correlation between morphological variables(diameter,perimeter,otolith length,otolith width,otolith area,density,harmonics 3,harmonics 4,harmonics 5,harmonics 13,harmonics 15,and harmonics 16) and body length.To minimize size effects on the morphological variables between species,only fish with a body length between 90 and 140 mm were included in the data analysis and the variables which had significant relation with body length were transformed using the residual.The result showed that the accuracy of discriminant analysis was 97.8% and 100% for Trumpeter sillago and Silver sillago,respectively.This high accuracy indicated that otolith shape was described accurately by morphological variables and Fourier harmonics,and discriminant analysis was an effective way to identify and separate the two species.展开更多
Using surplus production model packages of ASPIC(a stock-production model incorporating covariates) and CEDA(Catch effort data analysis),we analyzed the catch and effort data of Sillago sihama fishery in Pakistan.ASPI...Using surplus production model packages of ASPIC(a stock-production model incorporating covariates) and CEDA(Catch effort data analysis),we analyzed the catch and effort data of Sillago sihama fishery in Pakistan.ASPIC estimates the pa-rameters of MSY(maximum sustainable yield),Fmsy(fishing mortality),q(catchability coefficient),K(carrying capacity or unexploited biomass) and B1/K(maximum sustainable yield over initial biomass).The estimated non-bootstrapped value of MSY based on logistic was 598 t and that based on the Fox model was 415 t,which showed that the Fox model estimation was more conservative than that with the logistic model.The R2 with the logistic model(0.702) is larger than that with the Fox model(0.541),which indicates a better fit.The coefficient of variation(cv) of the estimated MSY was about 0.3,except for a larger value 88.87 and a smaller value of 0.173.In contrast to the ASPIC results,the R2 with the Fox model(0.651-0.692) was larger than that with the Schaefer model(0.435-0.567),indicating a better fit.The key parameters of CEDA are:MSY,K,q,and r(intrinsic growth),and the three error assumptions in using the models are normal,log normal and gamma.Parameter estimates from the Schaefer and Pella-Tomlinson models were similar.The MSY estimations from the above two models were 398 t,549 t and 398 t for normal,log-normal and gamma error distributions,re-spectively.The MSY estimates from the Fox model were 381 t,366 t and 366 t for the above three error assumptions,respectively.The Fox model estimates were smaller than those for the Schaefer and the Pella-Tomlinson models.In the light of the MSY estimations of 415 t from ASPIC for the Fox model and 381 t from CEDA for the Fox model,MSY for S.sihama is about 400 t.As the catch in 2003 was 401 t,we would suggest the fishery should be kept at the current level.Production models used here depend on the assumption that CPUE(catch per unit effort) data used in the study can reliably quantify temporal variability in population abundance,hence the mod-eling results would be wrong if such an assumption is not met.Because the reliability of this CPUE data in indexing fish population abundance is unknown,we should be cautious with the interpretation and use of the derived population and management parameters.展开更多
Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval stage,and the mechanism has not been understood thorough...Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval stage,and the mechanism has not been understood thoroughly.In this study,we investigated the transcriptome change in heart tissues under hypoxia stress.The fish were divided into four groups,including 1 h of hypoxia(hypoxia1h,dissolved oxygen(DO)=1.5±0.1 mg L^(−1)),4h of hypoxia(hypoxia4h,DO=1.5±0.1 mg L^(−1)),4h of reoxygen(reoxygen4h,DO=8.0±0.2 mg L^(−1))after 4h of hypoxia(DO=1.5 mg L^(−1))and normoxia or control(DO=8.0±0.2 mg L^(−1))groups.The results showed that a total of 3068 genes were identified as differentially expressed genes(DEGs)based on the criteria∣log2(Fold change)∣>1.0 and adjusted P-value<0.05.A total of 7761141 and 1151 DEGs were obtained from hypoxia1h,hypoxia4h and reoxygen4h groups,respectively.The enrichment pathway analysis showed that the DEGs were significantly enriched in ribosome biogenesis in eukaryotes,retinol metabolism,DNA replication and the oxidative phosphorylation(OXPHOS)pathways.Thirteen DEGs from the RNA-seq results were validated by quantitative real-time polymerase chain reaction(qRT-PCR).These candidate genes are considered as important regulatory factors involved in the hypoxia stress response in S.sihama.展开更多
The catch and effort data of Sillago sihama fishery in Pakistani waters were used to investigate the performance of two closely related stock assessment models: logistic and generalized surplus-production models. Comp...The catch and effort data of Sillago sihama fishery in Pakistani waters were used to investigate the performance of two closely related stock assessment models: logistic and generalized surplus-production models. Compared with the generalized production model, the logistic model produced more reasonable estimates for parameters such as maximum sustainable yield. The Akaike's Information Criterion values estimated at 4.265 and -51.152 respectively by the logistic and generalized models. Simulation analyses of the S. sihama fishery showed that the estimated and observed abundance indices for the logistic model were closer than those for the generalized production model. Standardized residuals were distributed closer for logistic model, but exhibited a slightly increasing trend for the generalized model. Statistical outliers were seen in 1989 and 1993 for the logistic model, and in 1981 and 1999 for the generalized model. Simulated results revealed that the logistic estimates were close to the true value for low CVs (coefficients of variation) but widely dispersed for high CVs. In contrast, the generalized model estimates were loose for all CV levels. The estimated production model curve parameter was not reasonable at all the tested levels of white noise. With the increase in white noise R2 for the catch per unit effort decreased. Therefore, we conclude that the logistic model performs more reasonably than the generalized production model.展开更多
Taking Sillago sihama Forssk?l as the research object,through the optimization of the extraction method,hydration and isoelectric focusing,a two-dimensional electrophoresis system was established for spleen proteome o...Taking Sillago sihama Forssk?l as the research object,through the optimization of the extraction method,hydration and isoelectric focusing,a two-dimensional electrophoresis system was established for spleen proteome of S. sihama Forssk?l. The results showed that the twpstep hydration and isoelectric focusing method is better than the hydration and isoelectric focusing integrated method. The protein spots of the spleen of S. sihama Forssk?l were detected by two-dimensional electrophoresis mainly at pH 4. 0-7. 0. The establishment of this technical system will lay a foundation for further research on proteomics of S. sihama Forssk?l in the future.展开更多
鱼类目标强度测量是渔业水声学工作的核心内容之一。本研究采用基尔霍夫近似模型对19尾多鳞鱚(Sillago sihama)样品的理论目标强度进行近似计算,模型计算所需鱼类形态学参数由中国水产科学研究院南海水产研究所所属X光机(SOFTEX M-100)...鱼类目标强度测量是渔业水声学工作的核心内容之一。本研究采用基尔霍夫近似模型对19尾多鳞鱚(Sillago sihama)样品的理论目标强度进行近似计算,模型计算所需鱼类形态学参数由中国水产科学研究院南海水产研究所所属X光机(SOFTEX M-100)拍摄X光影像获得。通过编程计算获得多鳞鱚不同频率下目标强度随姿态倾角的变化图案,并通过数据拟合方法建立了不同频率和倾角分布函数下多鳞鱚目标强度随体长变化的经验公式,并与常规的b20表式进行对比。结果表明:在70 kHz、120 kHz和200 kHz 3种频率下多鳞鱚目标强度的倾角变化图案呈多峰状特征分布,且频率越高目标强度对倾角变化越敏感,波峰数增加,目标强度最大值对应的倾角增大。70 k Hz下多鳞鱚目标强度最大值出现在–15°~5°,120 k Hz和200 k Hz下目标强度最大值则出现在–10°~0°,且各频率下目标强度最大值出现的位置各不相同。不同频率及倾角分布函数下多鳞鱚目标强度随体长变化特性各不相同,其中在角度函数为(–5°,15°)、频率为120 kHz,以及角度函数为(0°,10°)、频率为200 k Hz时目标强度对体长经验公式和常规的b_(20)表式曲线基本重合,拟合度较高,可将常规的b20表式直接用于多鳞鱚的资源评估;而其他情形下2种表式存在一定偏差,采用直接拟合的参数方程更为恰当。研究表明,基尔霍夫近似模型能够很好地反映多鳞鱚的目标强度特性,可为中国南海近岸鱼类目标强度研究提供有益借鉴,为提高渔业资源水声学评估的准确度和可信度提供科学依据。展开更多
文摘为构建多鳞[鱼喜](Sillago sihama)遗传连锁图谱并鉴定生长等重要经济性状数量性状位点(QTL),实验通过基因分型测序(GBS)技术对163个多鳞[鱼喜]个体(2个亲本和161个全同胞家系F1代)进行测序及标记分型,并通过复合区间定位法对该物种的体重、体高、体厚、眼径、体长和背鳍前长6个生长性状进行QTL定位分析。结果显示,多鳞[鱼喜]首张高密度遗传连锁图谱全长2 154.803 c M,标记间平均遗传距离0.455 c M,共有4 735个SNP标记分配到24个连锁群。QTL定位分析结果发现在6个生长性状中共检测到20个生长显著相关QTL位点,分布在8个连锁群上,单个QTL的LOD值范围为3.02~4.23,可解释的表型变异范围为0.14%~8.42%。其中,在连锁群LG08聚集了8个生长性状显著相关的QTL。通过对候选QTL区间内的基因进行功能注释,共筛选到了19个潜在生长调控相关基因,包含igf1、igf2、sstr5、sst1a、tgfbr2、gas1、igfals、gfg6、gfg20、bmp7、kdm5c、tti1以及rbm10等。实验获得的遗传标记及相关候选基因是多鳞[鱼喜]生长相关性状标记辅助选择(MAS)的有用基因资源,为进一步研究鱼类生长调控机制提供了更多的理论依据。
基金supported by the Natural Science Foundation of Shandong Province (Y2008D21)the National Basic Research Program of China (973 Program, No.2005CB422306)
文摘Otolith shape is species-specific in fish.In this study,161 samples of Trumpeter sillago Sillago maculata and 164 of Silver sillago Sillago sihama were collected from Beibu Gulf in July 2009.The main objective of this study was to use morphological variables and Fourier harmonics to describe otolith characteristics and use discriminant analyses to separate the two species.Otoliths were measured by traditional one and two dimensional measures(otolith length,width,diameters,area,perimeter,rectangularity and circularity),as well as by Fourier analysis to capture the finer regions of the otolith.Analysis of covariance(ANCOVA) showed that there was significant correlation between morphological variables(diameter,perimeter,otolith length,otolith width,otolith area,density,harmonics 3,harmonics 4,harmonics 5,harmonics 13,harmonics 15,and harmonics 16) and body length.To minimize size effects on the morphological variables between species,only fish with a body length between 90 and 140 mm were included in the data analysis and the variables which had significant relation with body length were transformed using the residual.The result showed that the accuracy of discriminant analysis was 97.8% and 100% for Trumpeter sillago and Silver sillago,respectively.This high accuracy indicated that otolith shape was described accurately by morphological variables and Fourier harmonics,and discriminant analysis was an effective way to identify and separate the two species.
基金supported by the special research fund ofthe Ocean University of China(201022001)the Chinese Scholarship Council(CSC)
文摘Using surplus production model packages of ASPIC(a stock-production model incorporating covariates) and CEDA(Catch effort data analysis),we analyzed the catch and effort data of Sillago sihama fishery in Pakistan.ASPIC estimates the pa-rameters of MSY(maximum sustainable yield),Fmsy(fishing mortality),q(catchability coefficient),K(carrying capacity or unexploited biomass) and B1/K(maximum sustainable yield over initial biomass).The estimated non-bootstrapped value of MSY based on logistic was 598 t and that based on the Fox model was 415 t,which showed that the Fox model estimation was more conservative than that with the logistic model.The R2 with the logistic model(0.702) is larger than that with the Fox model(0.541),which indicates a better fit.The coefficient of variation(cv) of the estimated MSY was about 0.3,except for a larger value 88.87 and a smaller value of 0.173.In contrast to the ASPIC results,the R2 with the Fox model(0.651-0.692) was larger than that with the Schaefer model(0.435-0.567),indicating a better fit.The key parameters of CEDA are:MSY,K,q,and r(intrinsic growth),and the three error assumptions in using the models are normal,log normal and gamma.Parameter estimates from the Schaefer and Pella-Tomlinson models were similar.The MSY estimations from the above two models were 398 t,549 t and 398 t for normal,log-normal and gamma error distributions,re-spectively.The MSY estimates from the Fox model were 381 t,366 t and 366 t for the above three error assumptions,respectively.The Fox model estimates were smaller than those for the Schaefer and the Pella-Tomlinson models.In the light of the MSY estimations of 415 t from ASPIC for the Fox model and 381 t from CEDA for the Fox model,MSY for S.sihama is about 400 t.As the catch in 2003 was 401 t,we would suggest the fishery should be kept at the current level.Production models used here depend on the assumption that CPUE(catch per unit effort) data used in the study can reliably quantify temporal variability in population abundance,hence the mod-eling results would be wrong if such an assumption is not met.Because the reliability of this CPUE data in indexing fish population abundance is unknown,we should be cautious with the interpretation and use of the derived population and management parameters.
基金This study was supported by grants from the National Natural Science Foundation of China(Nos.41706174 and 31702326)the Natural Science Foundation of Guangdong Province(No.2019A1515110619)+2 种基金the Department of Education of Guangdong Province(Nos.2018KQNCX111 and 2019KTSCX060)the College Students’Innovation and Entrepreneurship Project of Guangdong Province(No.CX XL2019138)the Program for Scientific Research Startup Funds of Guangdong Ocean University(No.R19026).
文摘Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval stage,and the mechanism has not been understood thoroughly.In this study,we investigated the transcriptome change in heart tissues under hypoxia stress.The fish were divided into four groups,including 1 h of hypoxia(hypoxia1h,dissolved oxygen(DO)=1.5±0.1 mg L^(−1)),4h of hypoxia(hypoxia4h,DO=1.5±0.1 mg L^(−1)),4h of reoxygen(reoxygen4h,DO=8.0±0.2 mg L^(−1))after 4h of hypoxia(DO=1.5 mg L^(−1))and normoxia or control(DO=8.0±0.2 mg L^(−1))groups.The results showed that a total of 3068 genes were identified as differentially expressed genes(DEGs)based on the criteria∣log2(Fold change)∣>1.0 and adjusted P-value<0.05.A total of 7761141 and 1151 DEGs were obtained from hypoxia1h,hypoxia4h and reoxygen4h groups,respectively.The enrichment pathway analysis showed that the DEGs were significantly enriched in ribosome biogenesis in eukaryotes,retinol metabolism,DNA replication and the oxidative phosphorylation(OXPHOS)pathways.Thirteen DEGs from the RNA-seq results were validated by quantitative real-time polymerase chain reaction(qRT-PCR).These candidate genes are considered as important regulatory factors involved in the hypoxia stress response in S.sihama.
基金supported by the special research fund of the Ocean University of China (No.201022001)
文摘The catch and effort data of Sillago sihama fishery in Pakistani waters were used to investigate the performance of two closely related stock assessment models: logistic and generalized surplus-production models. Compared with the generalized production model, the logistic model produced more reasonable estimates for parameters such as maximum sustainable yield. The Akaike's Information Criterion values estimated at 4.265 and -51.152 respectively by the logistic and generalized models. Simulation analyses of the S. sihama fishery showed that the estimated and observed abundance indices for the logistic model were closer than those for the generalized production model. Standardized residuals were distributed closer for logistic model, but exhibited a slightly increasing trend for the generalized model. Statistical outliers were seen in 1989 and 1993 for the logistic model, and in 1981 and 1999 for the generalized model. Simulated results revealed that the logistic estimates were close to the true value for low CVs (coefficients of variation) but widely dispersed for high CVs. In contrast, the generalized model estimates were loose for all CV levels. The estimated production model curve parameter was not reasonable at all the tested levels of white noise. With the increase in white noise R2 for the catch per unit effort decreased. Therefore, we conclude that the logistic model performs more reasonably than the generalized production model.
基金Natural Science Foundation of Guangdong Province (2017A030313174)Natural Science Foundation of Guangdong Ocean University (C17379)+1 种基金Undergraduate Innovative and Entrepreneurial Team Project (CCTD201802)Fishing Port Construction and Fishery Industry Development Special Fund of Guangdong Province (A201708A05)
文摘Taking Sillago sihama Forssk?l as the research object,through the optimization of the extraction method,hydration and isoelectric focusing,a two-dimensional electrophoresis system was established for spleen proteome of S. sihama Forssk?l. The results showed that the twpstep hydration and isoelectric focusing method is better than the hydration and isoelectric focusing integrated method. The protein spots of the spleen of S. sihama Forssk?l were detected by two-dimensional electrophoresis mainly at pH 4. 0-7. 0. The establishment of this technical system will lay a foundation for further research on proteomics of S. sihama Forssk?l in the future.
文摘鱼类目标强度测量是渔业水声学工作的核心内容之一。本研究采用基尔霍夫近似模型对19尾多鳞鱚(Sillago sihama)样品的理论目标强度进行近似计算,模型计算所需鱼类形态学参数由中国水产科学研究院南海水产研究所所属X光机(SOFTEX M-100)拍摄X光影像获得。通过编程计算获得多鳞鱚不同频率下目标强度随姿态倾角的变化图案,并通过数据拟合方法建立了不同频率和倾角分布函数下多鳞鱚目标强度随体长变化的经验公式,并与常规的b20表式进行对比。结果表明:在70 kHz、120 kHz和200 kHz 3种频率下多鳞鱚目标强度的倾角变化图案呈多峰状特征分布,且频率越高目标强度对倾角变化越敏感,波峰数增加,目标强度最大值对应的倾角增大。70 k Hz下多鳞鱚目标强度最大值出现在–15°~5°,120 k Hz和200 k Hz下目标强度最大值则出现在–10°~0°,且各频率下目标强度最大值出现的位置各不相同。不同频率及倾角分布函数下多鳞鱚目标强度随体长变化特性各不相同,其中在角度函数为(–5°,15°)、频率为120 kHz,以及角度函数为(0°,10°)、频率为200 k Hz时目标强度对体长经验公式和常规的b_(20)表式曲线基本重合,拟合度较高,可将常规的b20表式直接用于多鳞鱚的资源评估;而其他情形下2种表式存在一定偏差,采用直接拟合的参数方程更为恰当。研究表明,基尔霍夫近似模型能够很好地反映多鳞鱚的目标强度特性,可为中国南海近岸鱼类目标强度研究提供有益借鉴,为提高渔业资源水声学评估的准确度和可信度提供科学依据。
