期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Transcriptional activity and subcellular location of SmWRKY42-like and its response to gibberellin and ethylene treatments in Salvia miltiorrhiza hairy roots
1
作者 Zhen-qing Bai Wen-rui Li +1 位作者 Zi-yun Zhou Zong-suo Liang 《Chinese Herbal Medicines》 CAS 2018年第3期263-268,共6页
Objective: The exogenous gibberellin(GA) and ethylene(ET) treatment can improve the medicinal ingredients of Salvia miltiorrhiza. Interestingly, many reports pointed out that WRKY transcription factors played an impor... Objective: The exogenous gibberellin(GA) and ethylene(ET) treatment can improve the medicinal ingredients of Salvia miltiorrhiza. Interestingly, many reports pointed out that WRKY transcription factors played an important regulatory role in these treatment responses. However, whether the Sm WRKY mediate these treatment signalings in S. miltiorrhiza remains largely elusive.Methods: qRT-PCR was used for SmWRKY42-like in response to exogenous GA and ethephon(Eth) treatment. The subcellular location of SmWRKY42-like was transiently transformed into onion epidermal cells by particle bombardment. The self-activating activity of SmWRKY42-like was verified in AH109 yeast strain.Results: Sm WRKY42-like was a WRKY family gene in S. miltiorrhiza. The subcellular localization and transcriptional activity results of the SmWRKY42-like protein indicated that SmWRKY42-like mainly enriched in nucleus and might be a transcription factor in S. miltiorrhiza. In the meantime, the SmWRKY42-like gene significantly responded to exogenous GA and Eth treatment.Conclusion: These results collectively indicated the SmWRKY42-like gene functions, as an important hormone-responsive gene, might play a potentially role in ET and GA signaling pathways. 展开更多
关键词 ETHYLENE GIBBERELLINS Salvia miltiorrhiza Bunge smwrky42-like subcellular location
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部