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Hypermethylation of thymosinβ4 predicts a poor prognosis for patients with acute-on-chronic hepatitis B liver failure
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作者 He Wang Yan-Ping Yin +4 位作者 Zhen-Li Wang Yu Qian Yu-Chen Fan Hui-Hui Liu Kai Wang 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2023年第4期373-382,共10页
Background:It has been demonstrated that thymosinβ4(Tβ4)could inflect the severity of acute-on-chronic hepatitis B liver failure(ACHBLF),but the relationship between its methylation status and the prognosis of liver... Background:It has been demonstrated that thymosinβ4(Tβ4)could inflect the severity of acute-on-chronic hepatitis B liver failure(ACHBLF),but the relationship between its methylation status and the prognosis of liver failure is not clear.This study aimed to determine Tβ4 promoter methylation status in patients with ACHBLF and to evaluate its prognostic value.Methods:The study recruited 115 patients with ACHBLF,80 with acute-on-chronic hepatitis B pre-liver failure(pre-ACHBLF),and 86 with chronic hepatitis B(CHB).In addition,there were 36 healthy controls(HCs)from the Department of Hepatology,Qilu Hospital of Shandong University.The 115 patients with ACHBLF were divided into three subgroups:33 with early stage ACHBLF(E-ACHBLF),42 with mid-stage ACHBLF(M-ACHBLF),and 40 with advanced stage ACHBLF(A-ACHBLF).Tβ4 promoter methylation status in peripheral blood mononuclear cells(PBMCs)was measured by methylation-specific polymerase chain reaction,and mRNA was detected by quantitative real-time polymerase chain reaction.Results:Methylation frequency of Tβ4 was significantly higher in patients with ACHBLF than in those with pre-ACHBLF,CHB or HCs.However,expression of Tβ4 mRNA showed the opposite trend.In patients with ACHBLF,Tβ4 promoter methylation status correlated negatively with mRNA levels.The 3-month mortality of ACHBLF in the methylated group was significantly higher than that in the unmethylated group.Also,Tβ4 promoter methylation frequency was lower in survivors than in non-survivors.When used to predict the 1-,2-,and 3-month incidence of ACHBLF,Tβ4 methylation status was better than the model for end-stage liver disease(MELD)score.The predictive value of Tβ4 methylation was higher than that of MELD score for the mortality of patients with E-ACHBLF and M-ACHBLF,but not for A-ACHBLF.Conclusions:Tβ4 methylation might be an important early marker for predicting disease incidence and prognosis in patients with ACHBLF. 展开更多
关键词 Acute-on-chronic hepatitis B liver failure Acute-on-chronic hepatitis B pre-liver failure thymosinβ4 METHYLATION PROGNOSIS
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Preparation and in vitro release studies of thymosin-loaded PLA microspheres 被引量:2
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作者 何熠 肖国民 《Journal of Southeast University(English Edition)》 EI CAS 2007年第2期294-297,共4页
To obtain a kind of convenient oral dosage form of protein, which can be fully absorbed and is efficient and safe, the thymosin-loaded PLA(polylactic acid) microspheres are prepared by the emulsification- solvent ev... To obtain a kind of convenient oral dosage form of protein, which can be fully absorbed and is efficient and safe, the thymosin-loaded PLA(polylactic acid) microspheres are prepared by the emulsification- solvent evaporation method and the orthogonal design is used to optimize the technology of preparation. The form of the medicament microspheres of thymosin are proved by differential thermal analysis (DTA). The drug content is determined by the Lowry method, and the package ratio of medicament microspheres of thymosin and drug release in vitro are calculated. The results show that the average diameter and encapsulation efficiency of the product prepared according to the optimized formulation are 13. 8 μm and 80. 7%, respectively. The in vitro release behavior within 12 h can be described by the Higuchi equation with T1/2 = 295 rain. There are no significant changes in size distribution and residual drug contents after being stored at 25℃ and 40 ℃ for 90 d, respectively. Due to the fact that its thymosin content and package ratio meet the requirement, and its releasing half life is long, the thymosin-loaded PLA microsohere has a favorable application future. 展开更多
关键词 thymosin polylactic acid micro sphere in vitro release
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A randomized controlled clinical trial on the treatment of Thymosin-a1 versus interferon-α in patients with hepatitis B 被引量:48
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作者 Jing You Lin Zhuang Bao Zhang Tang Wei Bo Yang Su Ying Ding Wu Li Rong Xue Wu Hong Li Zhang Yan Mei Zhang Shao Ming Yan Lu Zhang ~1Department of Infectious Diseases,The First Affiliated Hospital of Kunming Medical College,Kunming 650032,Yunnan Province,China ~2Departrnent of Hepatology,Kunming Third Municipal People’s Hospital,Kunming 650041,Yunnan Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第3期411-414,共4页
INTRODUCTIONChronic hepatitis B virus (HBV) infection is a serious problem because of its world wide distribution and possible adverse sequelae ,such as cirrhosis and hepatocellular carcinoma .The World Health Organiz... INTRODUCTIONChronic hepatitis B virus (HBV) infection is a serious problem because of its world wide distribution and possible adverse sequelae ,such as cirrhosis and hepatocellular carcinoma .The World Health Organization estimates that HBV has infected mord than 350 million people worldwide ,and up to 20% of them will become chromic carricrs and will be at significant risk for cirrhosis and HCC .The ultimate goal of the therapy for chronic hepatitis B is to prevent progression to cirrhosis and to prevent development of HCC. 展开更多
关键词 HEPATITIS B/therapy thymosin INTERFERON-ALPHA HEPATITIS B virus randomized controlled trials
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家蝇Thymosin(THY)基因的克隆及原核表达 被引量:5
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作者 王宇 吴高吉 +4 位作者 罗曼 彭传林 修江帆 尚小丽 吴建伟 《生物技术通报》 CAS CSCD 北大核心 2015年第2期143-147,共5页
采用EST测序技术从构建的家蝇(Musca domestica)幼虫c DNA质粒文库中筛选到胸腺肽(Thymosin,THY)基因,以该基因的c DNA文库质粒为模板,设计引物,通过PCR扩增,测序鉴定,获得THY基因完整编码序列。运用生物信息学方法对该基因及其编码蛋... 采用EST测序技术从构建的家蝇(Musca domestica)幼虫c DNA质粒文库中筛选到胸腺肽(Thymosin,THY)基因,以该基因的c DNA文库质粒为模板,设计引物,通过PCR扩增,测序鉴定,获得THY基因完整编码序列。运用生物信息学方法对该基因及其编码蛋白的基本理化性质、信号肽和亚细胞定位等方面进行预测和分析。构建p ET-28a(+)-THY重组质粒,转化到大肠杆菌BL21(DE3)中进行诱导表达。研究结果表明,THY基因ORF全长384 bp,编码127个氨基酸,理论分子量14.3 k D,等电点为5.22,具有THY家族的蛋白保守结构域。构建重组原核质粒p ET-28a(+)-THY,经IPTG诱导,蛋白在大肠杆菌中获得表达,经亲和层析柱纯化获得目的蛋白,Western blot检测发现纯化的目的蛋白大小正确。 展开更多
关键词 家蝇 胸腺肽 克隆 原核表达
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Serum thymosin β4 levels in patients with hepatitis B virus-related liver failure 被引量:20
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作者 Tao Han,Ying Liu,Huan Liu,Zheng-Yan Zhu,Yan Li,Shi-Xiang Xiao,Zhen Guo,Zhen-Gang Zhao,Department of Hepatology,Tianjin Institute of Hepatobiliary Disease,Tianjin Key Laboratory of Artificial Cells,Tianjin Third Central Hospital,Tianjin Medical University,83 Jintang Road,Tianjin 300170, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第5期625-630,共6页
AIM:To investigate whether serum thymosinβ4 can provide diagnostic or prognostic information in liver failure patients caused by chronic hepatitis B virus(HBV) infection. METHODS:Serum thymosinβ4 levels were measure... AIM:To investigate whether serum thymosinβ4 can provide diagnostic or prognostic information in liver failure patients caused by chronic hepatitis B virus(HBV) infection. METHODS:Serum thymosinβ4 levels were measured in 30 patients with acute-on-chronic liver failure(ACLF), 31 patients with chronic liver failure(CLF),30 patients with compensated liver cirrhosis(CR)and 32 patients with chronic hepatitis B and 30 healthy controls.Serum thymosinβ4 levels were measured by enzyme-linked immunosorbent assay and Child-Pugh and model for end-stage liver disease(MELD)scores were calculated for each patient on admission.RESULTS:Compared with healthy controls,serum thymosinβ4 levels in ACLF,CLF,CR and chronic hepatitis B patients were significantly lower,6.5047 (4.7879-10.5314)μg/mL vs 0.4632(0.2759-0.8768) μg/mL,0.6981(0.5209-1.2008)μg/mL,1.8053 (0.8110-2.3397)μg/mL,3.7803(1.8570-6.4722)μg/mL, respectively(P<0.001).The levels of thymosinβ4 in liver failure(ACLF or CLF)patients were markedly lower than that in CR(P<0.001),and a difference was also found between CLF and ACLF patients(P=0.038).In patients with chronic liver disease,there was a positive relationship between thymosinβ4 levels and albumin, choline esterase,and platelet(P<0.001),and negative relationship with alanine aminotransferase(P=0.020), aspartate aminotransferase,total bilirubin,international normalized ratio of prothrombin time,and Child-Pugh and MELD scores(P<0.001).Of the 61 liver failure patients,the thymosinβ4 levels of non-survivors were significantly lower than that of survivors(P=0.007). Receiver operating characteristics analysis identified a thymosinβ4 cutoff level of 0.5708μg/mL for predicting poor prognosis in all liver failure patients.The serial thymosinβ4 values were observed in 13 liver failure inpatients.Lower initial values were observed in the death.While greater improvement in thymosinβ4 value was found in those who recovered from the disease. CONCLUSION:Serum thymosinβ4 can be used as an important potential predictor for liver failure caused by chronic HBV infection. 展开更多
关键词 thymosinβ4 Liver failure SERUM Hepatitis B virus BIOCHEMISTRY
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Recombinant adeno-associated virus carrying thymosin β4 suppresses experimental colitis in mice 被引量:6
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作者 Xiao-Yan Zheng Yi-Fei Lv +4 位作者 Shuang Li Qian Li Qian-Nan Zhang Xue-Ting Zhang Zhi-Ming Hao 《World Journal of Gastroenterology》 SCIE CAS 2017年第2期242-255,共14页
AIMTo investigate the protective effect of a recombinant adeno-associated virus carrying thymosin &#x003b2;<sub>4</sub> (AAV-T&#x003b2;<sub>4</sub>) on murine colitis via intracolonic a... AIMTo investigate the protective effect of a recombinant adeno-associated virus carrying thymosin &#x003b2;<sub>4</sub> (AAV-T&#x003b2;<sub>4</sub>) on murine colitis via intracolonic administration.METHODSAAV-T&#x003b2;<sub>4</sub> was prepared and intracolonically used to mediate the secretory expression of T&#x003b2;<sub>4</sub> in mouse colons. Dextran sulfate sodium (DSS) was applied to induce the murine ulcerative colitis, and 2,4,6-trinitrobenzene sulfonic acid (TNBS) was used to establish a mouse colitis model resembling Crohn&#x02019;s disease. The disease severity and colon injuries were observed and graded to reveal the effects of AAV-T&#x003b2;<sub>4</sub> on colitis. The activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were determined using biochemical assays. Colonic levels of tumor necrosis factor-&#x003b1; (TNF-&#x003b1;), interleukin (IL)-1&#x003b2; and IL-10 were measured using ELISA, and mucosal epithelial cell apoptosis and proliferation were detected by TUNEL assay and immunochemistry, respectively.RESULTSRecombinant AAVs efficiently delivered LacZ and T&#x003b2;<sub>4</sub> into the colonic tissues of the mice, and AAV-T&#x003b2;<sub>4</sub> led to a strong expression of T&#x003b2;<sub>4</sub> in mouse colons. In both the DSS and TNBS colitis models, AAV-T&#x003b2;<sub>4</sub>-treated mice displayed distinctly attenuated colon injuries and reduced apoptosis rate of colonic mucosal epithelia. AAV-T&#x003b2;<sub>4</sub> significantly reduced inflammatory cell infiltrations and relieved oxidative stress in the inflamed colons of the mice, as evidenced by decreases in MPO activity and MDA content and increases in SOD activity. AAV-T&#x003b2;<sub>4</sub> also modulated colonic TNF-&#x003b1;, IL-1&#x003b2; and IL-10 levels and suppressed the compensatory proliferation of colonic epithelial cells in DSS- and TNBS-treated mice.CONCLUSIONT&#x003b2;<sub>4</sub> exerts a protective effect on murine colitis, indicating that AAV-T&#x003b2;<sub>4</sub> could potentially be developed into a promising agent for the therapy of inflammatory bowel diseases. 展开更多
关键词 thymosin β 4 MICE COLITIS Dextran sulfate sodium 2 4 6-trinitrobenzene sulfonic acid
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Efficacy of thymosin alpha-1 and interferon alpha in treatment of chronic viral hepatitis B:A randomized controlled study 被引量:8
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作者 Jing You Lin Zhuang +11 位作者 Hong-Ying Cheng Shou-Ming Yan Lan Yu Jun-Hua Huang Bao-Zhang Tang Meng-Ling Huang Yong-Liang Ma Virasakdi Chongsuvivatwong Hutcha Sriplung Alan Geater Yan-Wei Qiao Rong-Xue Wu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第41期6715-6721,共7页
AIM: To observe the efficiency and safety of thymosin-α1 treatment in patients with hepatitis B e antigen (HBeAg) and HBV DNA positive chronic hepatitis. METHODS: Sixty-two patients were randomly divided into gro... AIM: To observe the efficiency and safety of thymosin-α1 treatment in patients with hepatitis B e antigen (HBeAg) and HBV DNA positive chronic hepatitis. METHODS: Sixty-two patients were randomly divided into groups A and B. The patients in group A received subcutaneous injection of 1.6 mg thymosin-α1, twice a week (T-α1 group) for six months, and the patients in group B received 5 MU interferon alpha (IFN-α) each day for fifteen days, then three times weekly (IFN-α group) for six months. The results between two groups treated with and the group untreated with IFN-α which was followed up for 12 mo (historical control group consisting of 30 patients) were compared, and three groups were comparable between each other (P 〉 0.05) at baseline (age, sex, clinical history, biochemical, and serological parameters). RESULTS: At the end of treatment, complete response, which was defined as alanine aminotransferase (ALT) normalization and HBV DNA and HBeAg loss, occurred in 9 of 29 (31.0%) patients in the T-α1 group and in 15 of 33 (45.5%) patients in the IFN-α group (χ^2= 1.36, P 〉 0.05). After a follow-up period of six months, a complete response was observed in 14 of 29 (48.3%) patients in the T-α1 group and in 9 of 33 (27.3%) patients in the IFN-α group (χ^2= 2.93, P 〉 0.05). Compared with the results observed in the historical control (HC) group untreated with IFN-α which was followed up for 12 mo, the rate of complete response was significantly higher in IFN-α group at the end of therapy (1 of 30 vs 15 of 33, 7:2 = 14.72, P 〈 0.001) and in the T-α1 group at the end of follow-up (1 of 30 vs 14 of 29,χ^2 = 15.71, P 〈 0.001). In T-α1 and IFN-α treatment groups, the area under (the plasma concentration time) curve (AUC) of negative HBV DNA and HBeAg was 340, 17%, 31% and 19% smaller than that in the HC group. By the end of the follow-up period, the proportions of ALT normalization and negative HBV DNA in the T-α1 group were significantly higher than those in the IFN-α and HC groups. The odds of ALT normalization and negative HBV DNA at the end of the follow-up was three-fold higher in the T-α1 group than in the IFN-α group. Unlike IFN-α, T-α1 was well tolerated by all patients, and no side effects appeared in T-α1 group. CONCLUSION: The results suggest that a 6-too course of T-α1 therapy is effective and safe in patients with chronic hepatitis B. T-α1 is able to reduce HBV replication in patients with chronic hepatitis B. Furthermore, T-α1 is better tolerated than IFN-α and can gradually induce more sustained ALT normalization and HBV DNA and HBeAg loss. However, a response rate of 48.3% is still less ideal. A more effective therapeutic approach warrants further study. 展开更多
关键词 Chronic hepatitis B EFFICACY Interferonalpha thymosin alpha-1
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Construction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion proteins in Pichia pastoris 被引量:5
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作者 You-FengYang Han-YingYuan Nan-SongLiu Xiang-LingChen Bu-YuGao HongLu Yu-YangLi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第17期2597-2602,共6页
AIM:Interferon α2b (IFNα2b) and thymosin α1 (Tα1) exhibit synergic effects in the treatment of hepatitis B and hepatitis C when used together. For developing a fusion protein drug, fusion proteins of IFNα2b and T... AIM:Interferon α2b (IFNα2b) and thymosin α1 (Tα1) exhibit synergic effects in the treatment of hepatitis B and hepatitis C when used together. For developing a fusion protein drug, fusion proteins of IFNα2b and Ta1 linked by different lengths of (G4S)n(n = 1-3) were constructed and expressed in Pichia pastoris. METHODS: Using PCR and molecular clone techniques, the fusion genes of IFNα2b-(G4S)n-Tα1 (n = 1-3) were constructed and subcloned into the eukaryotic expression vector pPIC9. After transformation of these plasmids into P. pastoris, the expressed fusion proteins IFNα2b-(G4S) n-Tα1 (n = 1-3) were obtained. These proteins were purified through diethylaminoethyl (DEAE) affinity chromatography and Superdex?75 gel filtration and analyzed by SDS-PAGE and Western blot. Antiviral and E-rosette assays were used to investigate the bioactivities of these fusion proteins. RESULTS: DNA sequencing confirmed that the fusion genes of IFNa2b-(G4S)n-Tα1 (n= 1-3) were correctly cloned to the pPIC9 vector. The recombinant IFNα2b-(G4S)n-Tα1 (n = 1-3) fusion proteins expressed in P. pastoris were purified with DEAE and Superdex?75 gel filtration chromatography. The fusion proteins could be observed on sodium dodecylsulfate-polyacrylamide gel electrophoresis with molecular weight (MW) of 23.2, 22.9, and 22.6 ku, respectively, and reacted to the IFNa2b monoclonal antibody and Tal polyclonal antibody. The purified fusion proteins exhibit antiviral activity and can enhance the percentage of E-rosette-forming-cell in E-rosette assay. CONCLUSION: The recombinant IFNa2b-(G4S)n-Tα1 (n = 1-3) fusion proteins were successfully expressed in P. pastoris. Purified fusion proteins exhibit both antiviral activity of IFNa2b and immunomodulatory activity of Tal in vitro. These results will be the basis for further evaluation of the fusion proteins' function in vivo. 展开更多
关键词 Fusion protein Interferon α2b thymosin al Antiviral assay E-rosette assay
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Preliminary results of Thymosin-a1 versus interferon-α treatment in patients with HBeAg negative and serum HBV DNA positive chronic hepatitis B 被引量:22
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作者 Lin Zhuang Jing You Bao Zhang Tang Su Ying Ding Kui Hua Yan Dan Peng Yan Mei Zhang Lu Zhang ~1Department of Hepatology,Kunming Third Municipal Peoples Hospital,Kunming 650041,Yunnan Province,China ~2Department of Infectious Diseases,The First Affiliated Hospital of Kunming Medical College,Kunming 650032,Yunnan Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第3期407-410,共4页
INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 a... INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 and C-myc protein expression in the development of gastric cancer . 展开更多
关键词 hepatitis B hepatitis B surface antigens INTERFERON-Α thymosin-a1 hepatitis B E antigens SEROLOGY
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Thymosin alpha 1:A comprehensive review of the literature 被引量:15
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作者 Asimina Dominari Donald Hathaway III +14 位作者 Krunal Pandav Wanessa Matos Sharmi Biswas Gowry Reddy Sindhu Thevuthasan Muhammad Adnan Khan Anoopa Mathew Sarabjot Singh Makkar Madiha Zaidi Michael Maher Mourad Fahem Renato Beas Valeria Castaneda Trissa Paul John Halpern Diana Baralt 《World Journal of Virology》 2020年第5期67-78,共12页
Thymosin alpha 1 is a peptide naturally occurring in the thymus that has long been recognized for modifying,enhancing,and restoring immune function.Thymosin alpha 1 has been utilized in the treatment of immunocompromi... Thymosin alpha 1 is a peptide naturally occurring in the thymus that has long been recognized for modifying,enhancing,and restoring immune function.Thymosin alpha 1 has been utilized in the treatment of immunocompromised states and malignancies,as an enhancer of vaccine response,and as a means of curbing morbidity and mortality in sepsis and numerous infections.Studies have postulated that thymosin alpha 1 could help improve the outcome in severely ill corona virus disease 2019 patients by repairing damage caused by overactivation of lymphocytic immunity and how thymosin alpha 1 could prevent the excessive activation of T cells.In this review,we discuss key literature on the background knowledge and current clinical uses of thymosin alpha 1.Considering the known biochemical properties including antibacterial and antiviral properties,timehonored applications,and the new promising findings regarding the use of thymosin,we believe that thymosin alpha 1 deserves further investigation into its antiviral properties and possible repurposing as a treatment against severe acute respiratory syndrome coronavirus-2. 展开更多
关键词 thymosin alpha 1 THYMALFASIN Immunomodulating T lymphocytes Infectious diseases Immune deficiency Oxidative damage
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Immunomodulatory function of orally administered thymosin α1 被引量:1
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作者 陈祥明 蒋汉梁 +5 位作者 周林福 潘小平 胡中荣 刘荣华 陈晓明 陈智 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第9期873-876,共4页
Objective: To investigate the immunological function of a yeast expression system for thymosin α1 (Tα1). Methods: A constructed Tetl yeast expression system was used to investigate the immunological function of ... Objective: To investigate the immunological function of a yeast expression system for thymosin α1 (Tα1). Methods: A constructed Tetl yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4^+ and CD8^+ levels were detected by flow cytometry assay. TNF-α, IFN-γ, IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results: In normal Balb/c mice or immune inhibition Balb/c mice, CD8^+ levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8^+ levels in synthesized Tα1 group (18.77%±4.72%), small dose group (13.48%±6.17%) and large dose group (22.74%±1.09%) were significantly higher than that in empty yeast control group (7.49%±2.14%). Conclusion: Orally administered Tα1 has its certain immunomodulatory function. 展开更多
关键词 thymosin α1 Yeast expression system Biological activity Orally administered
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Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection 被引量:5
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作者 MA Jinyou RUAN Lingwei +1 位作者 XU Xun GAO Zhaoming 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2016年第4期44-50,共7页
β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjt... β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags(EST) from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 c DNAs possessed open reading frames that encoded166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4,mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps.The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps. 展开更多
关键词 β-thymosin thymosin-repeat proteins white spot syndrome virus(WSSV) Marsupenaeus japonicus
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Thymosin <i>β</i>4 Improves Neurological Outcome and Enhances Induced Oligodendrogenesis in the Rat after ICH 被引量:1
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作者 Dongmei Yang Yuxia Han +1 位作者 Michael Chopp Donald M. Seyfried 《World Journal of Neuroscience》 2014年第5期395-405,共11页
Thymosin β4 (Tβ4), a G-actin binding protein, has diverse biological functions. This study tested the effects of Tβ4 on oligodendrogenesis in a rat model of intracerebral hemorrhage (ICH). ICH was induced by stereo... Thymosin β4 (Tβ4), a G-actin binding protein, has diverse biological functions. This study tested the effects of Tβ4 on oligodendrogenesis in a rat model of intracerebral hemorrhage (ICH). ICH was induced by stereotactic injection of 100 μm of autologous blood into the striatum in 32 male Wistar rats. The rats were randomly divided into four groups: 1) saline control group (n = 8);2) 3 mg/kg Tβ4-treated group (n = 8);3) 6 mg/kg Tβ4-treated group (n = 8);and 4) 12 mg/kg Tβ4treated group (n = 8). Tβ4 or saline was administered intraperitoneally starting at 24 h post ICH and then every 3 days for 4 additional doses. The neurological functional outcome was evaluated by behavioral tests (i.e., modified Neurological Severity Score and corner turn test) at multiple time points after ICH. Animals were sacrificed at 28 days post ICH, and histological studies were completed. Tβ4 treatment improved neurological functional recovery significantly and increased actively proliferating oligodendrocytic progenitor cells and myelinating oligodendrocytes in the ICH-affected brain tissue, compared with the saline-treated group. The high-dose treatment of Tβ4 showed better restorative effects compared with the low-dose treatment. Tβ4 treatment enhanced ICH-induced oligodendrogenesis that may contribute to the enhanced functional recovery after ICH. Further investigation is warranted to determine the associated underlying mechanisms of Tβ4 treatment for ICH. 展开更多
关键词 thymosin β4 NEUROGENESIS OLIGODENDROGENESIS INTRACEREBRAL Hemorrhage
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Thymosin as a possible therapeutic drug for COVID-19:A case report
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作者 Qiong Na Zheng Mei Yan Xu +2 位作者 Fang Min Gan Sha Sha Ye Hui Zhao 《World Journal of Clinical Cases》 SCIE 2021年第16期4090-4094,共5页
BACKGROUND There are no effective antiviral therapies for coronavirus disease 2019(COVID-19)at present.Although most patients with COVID-19 have a mild or moderate course of disease,up to 5%-10%of patients may have a ... BACKGROUND There are no effective antiviral therapies for coronavirus disease 2019(COVID-19)at present.Although most patients with COVID-19 have a mild or moderate course of disease,up to 5%-10%of patients may have a serious and potentially life-threatening condition,indicating an urgent need for effective therapeutic drugs.The therapeutic effect of thymosin on COVID-19 has not been previously studied.In this paper,for the first time we report a case of thymosin treatment of COVID-19.CASE SUMMARY A 51-year-old man with imported COVID-19 was admitted with definite symptoms of chest tightness,chest pain,and fatigue.The polymerase chain reaction results for severe acute respiratory syndrome coronavirus 2 were negative.The antibody test was positive,confirming the diagnosis of COVID-19.As many orally administered drugs were not well tolerated due to gastrointestinal symptoms,an emergency use of thymosin,a polypeptide consisting of 28 amino acids,was administered by injection.Finally,after the implementation of the treatment program,symptoms and lung imaging improved significantly.CONCLUSION In this case report,it is confirmed that thymosin may help alleviate the severity of COVID-19 symptoms. 展开更多
关键词 COVID-19 thymosin SARS-CoV-2 Treatment Therapeutic drug Case report
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Thymosin α-1能治疗乙型肝炎吗
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作者 邓永鸿 《生物技术通报》 CAS CSCD 1989年第8期11-11,共1页
目前,对慢性活性乙型肝炎还没有治疗方法。但 Thymosia α-l临床研究的初步材料提示,该肽(由华盛顿的 Alpha 1 Biomedicals 公司研制)可能治疗该疾病。Milton Mutchnick (密歇根州底特律的 Wayne州立大学)正主持这一研究。据称。
关键词 乙型肝炎 thymosin 密歇根州 ALPHA DNA 去氢可的松 SGPT 肝脏损伤 多中心试验 慢性肝炎
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Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris
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作者 曹俊霞 Jin +4 位作者 Liji Duan Yanlong An Lijia 《High Technology Letters》 EI CAS 2003年第3期60-64,共5页
The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replace... The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replaced by hT(1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thymosin α 1. And also,the Asn-Gly bond was designed to faciliate isolation of the target protein.The fusion gene was cloned into the expression vector, pPIC/9K. The constructs were transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully. 展开更多
关键词 EXPRESSION human thymosin α 1 Pichia pastoris
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Thymosin β4 promotes angiogenesis and increases muscle progenitor cell density in ischemic skeletal muscle in a mouse model of hind limb ischemia
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作者 Yue Zhou Eliana C. Martinez +2 位作者 Li-Ping Su Kok-Onn Lee Lei Ye 《Open Journal of Regenerative Medicine》 2012年第2期19-24,共6页
Aim: To determine the therapeutic effect of thy- mosin β4 (Tβ4) for treatment of ischemic limb disease in a mouse model. Methods: A mouse model of hindlimb ischemia was created by permanent ligation of femoral arter... Aim: To determine the therapeutic effect of thy- mosin β4 (Tβ4) for treatment of ischemic limb disease in a mouse model. Methods: A mouse model of hindlimb ischemia was created by permanent ligation of femoral arteries and internal iliac artery. Tβ4 was dissolved in sterile saline and intramuscularly injected into the centre and periphery of ligation area in the treatment group (n = 10) starting from the surgery day until 4 weeks after surgery, while control animals received saline injection only (n = 9). All animals were sacrificed at 6 weeks after surgery and used for immunohistochemistry studies. Results: Tβ4 stimulated angiogenesis was evidenced by increased vascular density based on CD31 immunostaining, which was sig- nifycantly increased in Tβ4 group (562.5 ± 78.4/mm2) as compared with control group (371.1 ± 125.7/mm2;p 0.05) groups. Tβ4 increased Pax3/7+ skeletal muscle progenitor cell density. Pax3/7+ cell density of Tβ4 group (13.7% ± 2%) was significantly higher than that of the control group (4.3% ± 1.6%, p < 0.05). However, the numbers of central nuclei fiber and central nuclei per fiber were insignificantly increased in Tβ4 group as compared to control group. The numbers of central nuclei fiber were 8.9 ± 2.1 and 9.5 ± 1.6, and the central nuclei per fiber were 0.25 ± 0.07 and 0.48 ± 0.09 for control and Tβ4 groups, respectively. Conclusions: This preliminary study suggests that localized delivery of Tβ4 increased angiogenesis and skeletal muscle progenitor cell density in ischemic skeletal muscle, but failed to promote skeletal muscle regeneration. 展开更多
关键词 thymosin β4 NEOVASCULARIZATION PROGENITOR Cell MUSCLE Regeneration
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Thymosinβ4 Impeded Murine Stem Cell Proliferation with an Intact Cardiovascular Differentiation
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作者 聂丽 高仕君 +7 位作者 赵亚楠 Jacob Masika 骆红艳 胡新武 张亮品 曾莹 Jürgen Hescheler 梁华敏 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2016年第3期328-334,共7页
Thymosin β4(Tβ4) is a key factor in cardiac development, growth, disease, epicardial integrity, blood vessel formation and has cardio-protective properties. However, its role in murine embryonic stem cells(m ESCs... Thymosin β4(Tβ4) is a key factor in cardiac development, growth, disease, epicardial integrity, blood vessel formation and has cardio-protective properties. However, its role in murine embryonic stem cells(m ESCs) proliferation and cardiovascular differentiation remains unclear. Thus we aimed to elucidate the influence of Tβ4 on m ESCs. Target genes during m ESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the m ESCs-derived cardiomyocytes. It was found that Tβ4 decreased m ESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun. However, m ESCs self-renewal markers Oct4 and Nanog were elevated, indicating the maintenance of self-renewal ability in these m ESCs. Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced. No significant difference was found in BMP2/BMP4 or their downstream protein smad. Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant ?-catenin. Under m ESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA. Neither the electrophysiological properties of m ESCs-derived cardiomyocytes nor the hormonal regulation by Iso/Cch was affected by Tβ4. In conclusion, Tβ4 suppressed m ESCs proliferation by affecting the activity of STAT3, Akt, ERK and Wnt pathways. However, Tβ4 did not influence the in vitro cardiovascular differentiation. 展开更多
关键词 thymosin β4 murine embryonic stem cells proliferation cardiogenesis patch clamp
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Effect of ulinastatin + thymosin adjuvant therapy on inflammatory and stress response in patients with severe pneumonia
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作者 Jiang Wang Ya-Dong Yang +1 位作者 Qiu-Fang She Gui-Fen Chen 《Journal of Hainan Medical University》 2017年第24期41-44,共4页
Objective: To investigate the effect of ulinastatin + thymosin adjuvant therapy on inflammatory and stress response in patients with severe pneumonia. Methods: A total of 66 patients with severe pneumonia who were dia... Objective: To investigate the effect of ulinastatin + thymosin adjuvant therapy on inflammatory and stress response in patients with severe pneumonia. Methods: A total of 66 patients with severe pneumonia who were diagnosed and treated in Huanggang Central Hospital between July 2016 and July 2017 were divided into control group (n=33) and study group (n=33) by random number table. Control group received routine therapy for severe pneumonia, and study group received routine therapy combined with ulinastatin + thymosin adjuvant therapy, which lasted for 1 week. The differences in serum levels of inflammatory factors and stress hormones were compared between the two groups before and after treatment. Results: Before treatment, the differences in serum levels of inflammatory factors and stress hormones were not statistically significant between the two groups. After 1 week of treatment, serum levels of inflammatory factors and stress hormones of both groups of patients were lower than those before treatment, serum levels of pro-inflammatory factors IL-1β, IL-2, IL-6, IL-8 and TNF-α as well as anti-inflammatory factors IL-4, IL-10 and IL-13 of study group were lower than those of control group, and serum stress hormones AngⅠ, AngⅡ, NE and Cor levels were lower than those of control group. Conclusion: Routine therapy combined with ulinastatin + thymosin adjuvant therapy can further inhibit the systemic inflammatory response and stress response and optimize the overall condition in patients with severe pneumonia. 展开更多
关键词 SEVERE PNEUMONIA ULINASTATIN thymosin INFLAMMATORY factor Stress HORMONE
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Thymosin β4可能减少心脏损害
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作者 王吉云 《国外药讯》 2005年第5期11-12,共2页
一篇在11月25日Nature杂志上发表的研究显示,由美国RegeneRx生物制药公司研发的Thymosin β4(Ⅰ)是一种天然生成的肽类物质,(Ⅰ)可明显减少组织损伤、心室的疤痕形成和心室扩大,并改善心脏功能。这一研究发现是在诱发小鼠心脏病后立... 一篇在11月25日Nature杂志上发表的研究显示,由美国RegeneRx生物制药公司研发的Thymosin β4(Ⅰ)是一种天然生成的肽类物质,(Ⅰ)可明显减少组织损伤、心室的疤痕形成和心室扩大,并改善心脏功能。这一研究发现是在诱发小鼠心脏病后立刻给予(Ⅰ)后观察到的。 展开更多
关键词 thymosin β4 心脏损害 美国 RegeneRx生物制药公司
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