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Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus
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作者 Binglei CAO Zhongyuan GE +3 位作者 Qi YANG Hang SUN Yu SUN Xiaohui SONG 《Agricultural Biotechnology》 2024年第4期21-27,共7页
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP... [Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value. 展开更多
关键词 Peste des petits ruminants N active protein NH fusion protein Soluble expression and purification time-resolved fluorescence immunoassay
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Assembling Tunable Time-Resolved Fluorescence Layer onto Nano-Gold
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作者 贺全国 张永强 聂立波 《Journal of Rare Earths》 SCIE EI CAS CSCD 2007年第2期152-157,共6页
The assembling of a coating of time-resolved fluorescent chelator BSPDA ( abbreviated for 4, 7-bis ( sulfhydrylphenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) onto a nano-gold layer was demonstrated. First, B... The assembling of a coating of time-resolved fluorescent chelator BSPDA ( abbreviated for 4, 7-bis ( sulfhydrylphenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) onto a nano-gold layer was demonstrated. First, BSPDA was synthesized by simple procedures, and then an approach was developed to immobilize BSPDA onto the nano-gold layer deposited on a silane modified glass substrate, whereby europium ion (Ⅲ, Eu^3+ ) was captured and released owing to the interactive process of complexation and dissociation between BSPDA functionalized coating and Eu^3+ solution. The fluorescence spectra and related lifetimes were determined. Also, the BSPDA functionalized coating's specific complexation with Eu^3+ on the BSPDA assembly layer and the nonspecific adsorption of Eu^3+ on the nano-gold layer were compared. These results allowed a selective complexation of Eu^3+ by assembling a BSPDA chelating layer on the nano-gold layer; thus, a tunable time-resolved fluorescent layer was covalently attached, The results of the nanoparticle assembling and probing (or labeling) processes to specific bio-systems were very interesting and had significant implications to time-resolved-fluorescence-based detection on biosensor surfaces such as DNA chip and to arrayed light display devices. 展开更多
关键词 assembly 4 7-bis sulfhydrylphenyl )-1 10-phenanthroline-2 9-dicarboxylic acid (BSPDA) EUROPIUM time-resolved fluorescence NANO-GOLD comolexation
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An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B1 in edible oil
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作者 Du Wang Zhaowei Zhang +5 位作者 Peiwu Li Qi Zhang Jin Mao Li Yu Wen Zhang Xiaoxia Ding 《Oil Crop Science》 2016年第3期57-64,共8页
Edible oil is one major nutritional ingredient to human and widely consumed directly. The contamination of aflatoxin B1 (AFB1) in edible oils has been attracted exten-sive efforts due to its hazard to human health a... Edible oil is one major nutritional ingredient to human and widely consumed directly. The contamination of aflatoxin B1 (AFB1) in edible oils has been attracted exten-sive efforts due to its hazard to human health and life. To avoid the digestion of edible oils contaminated by AFB1 the development of rapid and sensitive sensing method for AFB1 is required. Herein, a quantitative, sensitive and rapid method for AFB1 detection in edible oils was proposed by using ultrasensitive time-resolved fluorescent immunosensing (TRFIS) method. This method poses unique advantages from both time-resolved fluorescent sens-ing method and immunochromatographic assay format. The nanospheres were modified with fluorescent europium and then captured the home-made monoclonal antibody against AFB1 (3G1). After optimization, by using a competitive immunosensing manner, this TRFIS method has a detectable linear range of 0.54-20.0 μg/kg with minimum detectable concen-tration of 0.18μg/kg. It can be completed merely within 10 min with recovery from 87.0% to 121.9%. The agreement was observed between the results by TRFIS and high perfor-mance liquid chromatography (HPLC) methods. This research provides a promising sens-ing method for sensitive and rapid determining AFB1 in edible oils. 展开更多
关键词 time-resolved fluorescent immunosensing (TRFIS) europium label aflatoxin B1 (AFB1) edible oils
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The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection 被引量:11
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作者 SHENG Ya Jie EREMIN Sergei +3 位作者 MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第2期126-129,共4页
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed... A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs. 展开更多
关键词 FPIA AFB The Development of A fluorescence Polarization immunoassay for Aflatoxin Detection AFM EDF
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3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents
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作者 Dmitry E. Pugachev Georgy V. Zatonsky +2 位作者 Tatyana S. Kostryukova Anna G. Shubina Nikolay V. Vasiliev 《International Journal of Organic Chemistry》 2024年第1期20-31,共12页
New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carb... New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region. 展开更多
关键词 fluorescence immunoassay Fluorinated β-Diketones CARBAZOLE Europium Complexes STREPTAVIDIN Nanodispersions
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Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection
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作者 JianQiuMI XiaoHuaQI XinXiangZHANG WenBaoCHANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第8期943-946,共4页
N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals. With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine ... N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals. With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers. The detection limit was calculated to be 40 ng/mL. Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers. 展开更多
关键词 Polyclonal antibody MORPHINE capillary electrophoresis immunoassay (CEIA) laser-induced fluorescence (LIF) specific.
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Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone
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作者 ZHANG Xuan WANG Qiang +5 位作者 YU Zhong Sergei A. Eremin YU Chun Fai LIU Jin SUN Yuan Ming LEI Hong Tao 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第1期52-55,共4页
Estrone has been identified as a potential endocrine-disrupting chemical (EDC)[1]. Estrone is usually quantified by gas chromatography-mass spectrometry (GC-MS), GC-MS/MS, high performance liquid chromatography (... Estrone has been identified as a potential endocrine-disrupting chemical (EDC)[1]. Estrone is usually quantified by gas chromatography-mass spectrometry (GC-MS), GC-MS/MS, high performance liquid chromatography (HPLC), HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay (ELISA) or chemiluminescence immunoassay (CLIA) for determination of estrone in real samples have been developed[2'4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods (with separation) to homogeneous assays (without separation)[5]. Fluorescence polarization immunoassay (FPIA) is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production. 展开更多
关键词 FPIA Antibody Production for a Rapid fluorescence Polarization immunoassay of Estrone
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A QUENCHING FLUORESCENCE IMMUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED TERBIUM CHELATE
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作者 Feng Ji YAO Pei Hong LI Xiao Da YANG Bo XING Yun Xiang CI (Department of Chemistry,Peking University,100871) 《Chinese Chemical Letters》 SCIE CAS CSCD 1991年第9期737-738,共2页
A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process ... A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process is unnecessary.The recovery of HSA and albumin in urine is 107% and 95% respectively.The standard deviation is tess than 10%. 展开更多
关键词 FIA A QUENCHING fluorescence immunoassay METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED TERBIUM CHELATE
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Time-resolved Luminescence-based Chemosensor for Fluoride Anion
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作者 Mei SHI Xiang Hong LI Fu You LI Deng Qing ZHANG Bing Bing DAI Tao YI Chun Hui HUANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第1期69-72,共4页
A new europium complex is descried as a time-resolved luminescence-based sensor for fluoride anion. The sensor is selective even in the presence of intensive background fluorescence.
关键词 fluorescent chemosensor time-resolved luminescence fluoride anion.
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Preliminary Study of the CAS-LIBB Single-Particle Microbeam Ⅱ Endstation: Ⅰ. Proposed Multi-Dimensional Quantitative Fluorescence Microscopy
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作者 胡智文 许永建 余增亮 《Plasma Science and Technology》 SCIE EI CAS CSCD 2006年第3期366-371,共6页
Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioen... Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS), China. However there has been less research activities in this field concerning the original process of the interaction between low-energy ions and complicated organisms. To address this challenge, an in situ multi-dimensional quantitative fluorescence microscopy system combined with the CAS-LIBB single-particle microbeam II endstation is proposed. In this article, the rationale, logistics and development of many aspects of the proposed system are discussed. 展开更多
关键词 single-particle microbeam quantitative fluorescence microscopy cell irradiation time-resolved analysis
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Intense Long-Lived Fluorescence of 1,6-Diphenyl-1,3,5-Hexatriene: Emission from the S<sub>1</sub>-State Competes with Formation of O<sub>2</sub>Contact Charge Transfer Complex
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作者 Katharina Hunger Karl Kleinermanns 《Open Journal of Physical Chemistry》 2013年第1期59-67,共9页
The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μ... The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μM and excitation energies above 1 mJ a long-lived, very intense emission, which appears within less than 5 ns and lasts up to 70 ns, is observed. During the first 50 ns the decay does not follow an exponential but rather a linear behaviour. In oxygen saturated solutions the long-lived emission is suppressed and solely short-lived fluorescence with τ 1-state and competes with the formation of DPH-O2 contact charge-transfer complexes and intersystem crossing which both quench the fluorescence. Our investigations show that even the small amount of oxygen dissolved in nitrogen saturated solutions has a distinct influence on the fluorescence kinetics of DPH. 展开更多
关键词 DPH time-resolved fluorescence Simulations Kinetics Diphenylpolyenes
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动物源食品中孕酮激素残留的时间分辨荧光免疫分析法的建立
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作者 刘波 华彦涛 +3 位作者 马楠楠 赵炫 陈丽楠 袁利鹏 《现代食品科技》 CAS 北大核心 2024年第6期279-284,共6页
该研究以孕酮为研究对象,设计并制备了新抗原,通过动物免疫得到特异性兔抗体,基于所得抗原和抗体,进一步优化了抗原抗体浓度、反应缓冲液种类及其pH值等关键参数,最终在国内首次建立了用于动物性食品中性激素孕酮残留快速检测时间分辨... 该研究以孕酮为研究对象,设计并制备了新抗原,通过动物免疫得到特异性兔抗体,基于所得抗原和抗体,进一步优化了抗原抗体浓度、反应缓冲液种类及其pH值等关键参数,最终在国内首次建立了用于动物性食品中性激素孕酮残留快速检测时间分辨荧光免疫分析法(TRFIA)。该研究确定最优实验条件为:抗原0.30μg/mL、铕标抗体0.40μg/mL、pH值7.2的TBST工作缓冲液,并建立标准曲线,方法的线性范围为0.46~6.94μg/L,IC_(50)达1.79μg/L,与睾酮交叉反应率为1.25%,与雌二醇、雌三醇等激素无交叉反应。实际样品的平均添加回收率为82.0%~113.0%,批内批间变异系数<5%,与高效液相色谱法(HPLC)检测结果的相关性良好(r2=0.988)。结果表明该研究所建立的TRFIA检测方法准确可靠、特异性好、灵敏度高,完全可用于动物源食品中孕酮残留的快速检测。 展开更多
关键词 动物性食品 孕酮 抗体 时间分辨荧光免疫分析 检测
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时间分辨荧光免疫分析仪的校准方法研究
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作者 周瑾艳 武利庆 +6 位作者 黄彦捷 王晶 黄哲峰 李志雄 孙雅玲 许俊斌 陈玲 《中国测试》 CAS 北大核心 2024年第S01期305-310,共6页
研究一种适用于时间分辨荧光原理的免疫分析仪校准方法。经过对时间分辨荧光免疫分析仪荧光特性、孵育舱温度、分析项目浓度示值误差,重复性等性能指标的实验和研究,建立该分析仪的校准方法。起草该分析仪的校准规范,提出了仪器的校准... 研究一种适用于时间分辨荧光原理的免疫分析仪校准方法。经过对时间分辨荧光免疫分析仪荧光特性、孵育舱温度、分析项目浓度示值误差,重复性等性能指标的实验和研究,建立该分析仪的校准方法。起草该分析仪的校准规范,提出了仪器的校准项目与技术指标,确定了校准用的仪器设备,设计了完备的校准方案,使时间分辨荧光免疫分析仪的量值准确、可靠、可溯源,为统一相关量值发挥重要的作用。 展开更多
关键词 时间分辨 荧光 免疫分析
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用于诺氟沙星现场灵敏检测的便携式智能手机赋能荧光传感技术
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作者 卓雨欣 徐文娟 +1 位作者 程源 龙峰 《中国环境科学》 EI CAS CSCD 北大核心 2024年第1期482-488,共7页
通过融合激光诱导荧光原理和光纤传感原理,集成全光纤光学系统、微型化流动进样系统、高灵敏微型光电探测系统和基于智能手机的APP软件,研制了用于水中抗生素现场快速灵敏检测的便携式智能手机赋能荧光生物传感器.以喹诺酮类抗生素诺氟... 通过融合激光诱导荧光原理和光纤传感原理,集成全光纤光学系统、微型化流动进样系统、高灵敏微型光电探测系统和基于智能手机的APP软件,研制了用于水中抗生素现场快速灵敏检测的便携式智能手机赋能荧光生物传感器.以喹诺酮类抗生素诺氟沙星(NOR)为例,在优化条件下,该荧光生物传感器对NOR的检测限可达0.35μg/L,线性检测范围为1.6~20.6µg/L.所建立的NOR检测方法也被用于各种水样的NOR加标回收检测,其回收率为85%~120%,检测时间少于15min,表明其可以用于实际样品中NOR的现场灵敏快速检测.结合物联网等新兴技术,可根据设定的程序将检测结果直接上传环境监测监管中心,为实现水环境安全的监测预警和应急监测提供重要技术支持. 展开更多
关键词 抗生素 生物传感器 免疫分析 智能手机 激光诱导荧光 新污染物
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Developing a fluorescence substrate for HRP-based diagnostic assays with superiorities over the commercial ADHP
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作者 Zhichao Zhou Fuqian Chen +6 位作者 Xiaotong Xia Dong Ye Rong Zhou Lei Li Tao Deng Zhenhua Ding Fang Liu 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第6期439-444,共6页
The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or A... The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or Amplex red)is the most sensitive fluorogenic substrate known for HRP in current market,however,it suffers from some drawbacks,such as non-specific reactivity to carboxylesterase and limited fluorescence stability.In the present study,a novel HRP substrate10-cyclopropylcarbonyl-dichloro-dihydroxyphenoxazine(AR-2),has been prepared,which exhibited improved sensitivity than ADHP in sensing HRP.Moreover,the fluorescence of AR-2/HRP demonstrated improved tolerance to physiological relevant p H fluctuation as compared to ADHP/HRP.Successful detection of uric acid/urate oxidase reaction indicated excellent application prospect of AR-2/HRP for monitoring H_(2)O_(2)-generating biochemical reactions.More interestingly,an enzyme-linked immunosorbent assay(ELISA)using AR-2 as the fluorescence reporter has been successfully used in detecting IgG against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human serum samples.Overall,AR-2 exhibits improved performances over the commercial ADHP,which will be an ideal alternative to ADHP in HRP-based fluorescence biochemical analysis and immunoassays. 展开更多
关键词 fluorescence immunoassay HRP fluorescence substrate Hydroresorufin Amplex red/ADHP SARS-CoV-2
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Effect of Deposition Temperature on Optical Properties of Porous Amorphous SiC Film
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作者 徐吉祥 TAO Weijie +3 位作者 LIU Canhui XU Haoyao LI Lixuan 贺振华 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第4期839-844,共6页
To study the effect of different deposition temperatures on the optical properties of porous SiC films,single crystal Si was used as the substrate,a layer of anodic aluminum oxide(AAO)film was transferred on the Si su... To study the effect of different deposition temperatures on the optical properties of porous SiC films,single crystal Si was used as the substrate,a layer of anodic aluminum oxide(AAO)film was transferred on the Si substrate by chemical method,and then a layer of SiC was deposited on anodic aluminum oxide(AAO)template to prepare porous fluorescent SiC film by magnetron sputtering.The deposition temperature was ranged from 373 to 873 K.The thickness of the porous SiC film coated on the AAO surface was around 283 nm.It is found that the porous SiC with the deposition temperature of 873 K has the strongest photoluminescence(PL)intensity excited by 375 nm laser.The time-resolved PL spectra prove that the PL is mainly from intrinsic light emitting of SiC.With the optimized process,porous amorphous SiC film may have potential applications in the field of warm white LEDs. 展开更多
关键词 porous SiC film fluorescent SiC anodic aluminum oxide magnetron sputtering time-resolved spectrum
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我国免疫分析仪发展现状与趋势研究 被引量:1
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作者 黄芝香 钱文文 +1 位作者 郑建 徐向彩 《中国食品药品监管》 2024年第3期114-121,共8页
免疫分析技术作为疾病分析的重要手段,一直是生命科学领域研究的重点。近些年随着科学技术的不断发展,免疫诊断已逐渐成为体外诊断行业最大的细分领域。免疫分析仪作为免疫诊断的定量分析仪器,其发展规模、技术水平影响着免疫诊断行业... 免疫分析技术作为疾病分析的重要手段,一直是生命科学领域研究的重点。近些年随着科学技术的不断发展,免疫诊断已逐渐成为体外诊断行业最大的细分领域。免疫分析仪作为免疫诊断的定量分析仪器,其发展规模、技术水平影响着免疫诊断行业高质量快速发展的程度。本文概述了免疫分析仪行业的发展现状,分析了各细分领域的注册情况以及竞争格局,总结了免疫分析仪行业存在的问题,并对行业的未来发展趋势进行了展望。 展开更多
关键词 免疫分析仪 免疫诊断 体外诊断 化学发光免疫分析 荧光免疫分析
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Time-Resolved Imaging in Short-Wave Infrared Region
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作者 徐杨 李万万 《Journal of Shanghai Jiaotong university(Science)》 EI 2024年第1期29-36,共8页
Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scatter... Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scattering background,leading to great potential for in vivo imaging.Based on the limitations of the common spectral domain,and the superiority of the time-dimension,time-resolved imaging eliminates the auto-fuorescence in the biological tissue,thus supporting higher signal-to-noise ratio and sensitivities.The imaging technique is not affected by the difference in tissue composition or thickness and has the practical value of quan-titative in vivo detection.Almost all the relevant time-resolved imaging was carried out around lanthanide-doped upconversion nanomaterials,owing to the advantages of ultralong luminescence lifetime,excellent photostability,controllable morphology,easy surface modification and various strategies of regulating lifetime.Therefore,this review presents the research progress of SWIR time-resolved imaging technology based on nanomaterials doped with lanthanide ions as luminescence centers in recent years. 展开更多
关键词 time-resolved imaging short-wave infrared fluorescence probe lanthanide ions
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新型均相免疫分析技术——荧光淬体免疫探针分析
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作者 赵广伟 陈浩 董金华 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第10期2223-2228,共6页
生化分析在疾病诊断、环境污染源鉴定、食品安全等多个领域具有重要意义。基于抗原抗体反应的免疫检测技术具有高特异性与多样性等特点,其操作比基于大型设备的色谱法简单,检测对象选择性更好,广泛应用于样品检测。荧光淬体免疫探针(Q-b... 生化分析在疾病诊断、环境污染源鉴定、食品安全等多个领域具有重要意义。基于抗原抗体反应的免疫检测技术具有高特异性与多样性等特点,其操作比基于大型设备的色谱法简单,检测对象选择性更好,广泛应用于样品检测。荧光淬体免疫探针(Q-body)是一种通过荧光染料标记抗体片段构建成的新型荧光免疫检测探针,抗体内部色氨酸导致荧光基团发生电子转移而淬灭,当与抗原特异性结合时,其荧光强度恢复并呈浓度依赖性。因此,可通过检测Q-body的荧光强度变化检测抗原浓度。Q-body免疫检测技术因操作简便及灵敏度高等特点,被广泛应用于环境污染物质及疾病标志物的快速检测,对环境检测及疾病诊断具有重要意义。本文从Q-body的原理、构建方法、检测技术的开发及国内外研究进展进行综合论述,并对该技术的未来应用做出展望。 展开更多
关键词 抗体 免疫检测 荧光探针 淬灭 疾病标志物
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免疫测定法检测农药残留的研究进展
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作者 李爽 赵云珠 +3 位作者 王明慧 姜欣彤 吴昊璁 何思涵 《食品研究与开发》 CAS 2024年第21期210-216,共7页
农药除被作物靶向部位吸收外,剩余部分的农药富集、渗透到土壤或空气中。因此,利用可靠、准确且有效的检测技术来监控农药残留尤为重要。该文综述检测不同种类农药残留的免疫分析方法,包括酶联免疫吸附分析法、荧光免疫分析法、化学发... 农药除被作物靶向部位吸收外,剩余部分的农药富集、渗透到土壤或空气中。因此,利用可靠、准确且有效的检测技术来监控农药残留尤为重要。该文综述检测不同种类农药残留的免疫分析方法,包括酶联免疫吸附分析法、荧光免疫分析法、化学发光免疫分析法、免疫层析法和放射免疫分析法,对上述技术的基本原理和用途进行讨论和对比,并阐述不同种类农药免疫测定技术的发展潜力与趋势。 展开更多
关键词 农药残留 酶联免疫吸附分析 荧光免疫分析 化学发光免疫分析 免疫层析 放射免疫分析
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