[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
The assembling of a coating of time-resolved fluorescent chelator BSPDA ( abbreviated for 4, 7-bis ( sulfhydrylphenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) onto a nano-gold layer was demonstrated. First, B...The assembling of a coating of time-resolved fluorescent chelator BSPDA ( abbreviated for 4, 7-bis ( sulfhydrylphenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) onto a nano-gold layer was demonstrated. First, BSPDA was synthesized by simple procedures, and then an approach was developed to immobilize BSPDA onto the nano-gold layer deposited on a silane modified glass substrate, whereby europium ion (Ⅲ, Eu^3+ ) was captured and released owing to the interactive process of complexation and dissociation between BSPDA functionalized coating and Eu^3+ solution. The fluorescence spectra and related lifetimes were determined. Also, the BSPDA functionalized coating's specific complexation with Eu^3+ on the BSPDA assembly layer and the nonspecific adsorption of Eu^3+ on the nano-gold layer were compared. These results allowed a selective complexation of Eu^3+ by assembling a BSPDA chelating layer on the nano-gold layer; thus, a tunable time-resolved fluorescent layer was covalently attached, The results of the nanoparticle assembling and probing (or labeling) processes to specific bio-systems were very interesting and had significant implications to time-resolved-fluorescence-based detection on biosensor surfaces such as DNA chip and to arrayed light display devices.展开更多
Edible oil is one major nutritional ingredient to human and widely consumed directly. The contamination of aflatoxin B1 (AFB1) in edible oils has been attracted exten-sive efforts due to its hazard to human health a...Edible oil is one major nutritional ingredient to human and widely consumed directly. The contamination of aflatoxin B1 (AFB1) in edible oils has been attracted exten-sive efforts due to its hazard to human health and life. To avoid the digestion of edible oils contaminated by AFB1 the development of rapid and sensitive sensing method for AFB1 is required. Herein, a quantitative, sensitive and rapid method for AFB1 detection in edible oils was proposed by using ultrasensitive time-resolved fluorescent immunosensing (TRFIS) method. This method poses unique advantages from both time-resolved fluorescent sens-ing method and immunochromatographic assay format. The nanospheres were modified with fluorescent europium and then captured the home-made monoclonal antibody against AFB1 (3G1). After optimization, by using a competitive immunosensing manner, this TRFIS method has a detectable linear range of 0.54-20.0 μg/kg with minimum detectable concen-tration of 0.18μg/kg. It can be completed merely within 10 min with recovery from 87.0% to 121.9%. The agreement was observed between the results by TRFIS and high perfor-mance liquid chromatography (HPLC) methods. This research provides a promising sens-ing method for sensitive and rapid determining AFB1 in edible oils.展开更多
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed...A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.展开更多
New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carb...New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.展开更多
N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals. With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine ...N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals. With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers. The detection limit was calculated to be 40 ng/mL. Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.展开更多
Estrone has been identified as a potential endocrine-disrupting chemical (EDC)[1]. Estrone is usually quantified by gas chromatography-mass spectrometry (GC-MS), GC-MS/MS, high performance liquid chromatography (...Estrone has been identified as a potential endocrine-disrupting chemical (EDC)[1]. Estrone is usually quantified by gas chromatography-mass spectrometry (GC-MS), GC-MS/MS, high performance liquid chromatography (HPLC), HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay (ELISA) or chemiluminescence immunoassay (CLIA) for determination of estrone in real samples have been developed[2'4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods (with separation) to homogeneous assays (without separation)[5]. Fluorescence polarization immunoassay (FPIA) is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.展开更多
A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process ...A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process is unnecessary.The recovery of HSA and albumin in urine is 107% and 95% respectively.The standard deviation is tess than 10%.展开更多
A new europium complex is descried as a time-resolved luminescence-based sensor for fluoride anion. The sensor is selective even in the presence of intensive background fluorescence.
Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioen...Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS), China. However there has been less research activities in this field concerning the original process of the interaction between low-energy ions and complicated organisms. To address this challenge, an in situ multi-dimensional quantitative fluorescence microscopy system combined with the CAS-LIBB single-particle microbeam II endstation is proposed. In this article, the rationale, logistics and development of many aspects of the proposed system are discussed.展开更多
The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μ...The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μM and excitation energies above 1 mJ a long-lived, very intense emission, which appears within less than 5 ns and lasts up to 70 ns, is observed. During the first 50 ns the decay does not follow an exponential but rather a linear behaviour. In oxygen saturated solutions the long-lived emission is suppressed and solely short-lived fluorescence with τ 1-state and competes with the formation of DPH-O2 contact charge-transfer complexes and intersystem crossing which both quench the fluorescence. Our investigations show that even the small amount of oxygen dissolved in nitrogen saturated solutions has a distinct influence on the fluorescence kinetics of DPH.展开更多
The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or A...The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or Amplex red)is the most sensitive fluorogenic substrate known for HRP in current market,however,it suffers from some drawbacks,such as non-specific reactivity to carboxylesterase and limited fluorescence stability.In the present study,a novel HRP substrate10-cyclopropylcarbonyl-dichloro-dihydroxyphenoxazine(AR-2),has been prepared,which exhibited improved sensitivity than ADHP in sensing HRP.Moreover,the fluorescence of AR-2/HRP demonstrated improved tolerance to physiological relevant p H fluctuation as compared to ADHP/HRP.Successful detection of uric acid/urate oxidase reaction indicated excellent application prospect of AR-2/HRP for monitoring H_(2)O_(2)-generating biochemical reactions.More interestingly,an enzyme-linked immunosorbent assay(ELISA)using AR-2 as the fluorescence reporter has been successfully used in detecting IgG against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human serum samples.Overall,AR-2 exhibits improved performances over the commercial ADHP,which will be an ideal alternative to ADHP in HRP-based fluorescence biochemical analysis and immunoassays.展开更多
To study the effect of different deposition temperatures on the optical properties of porous SiC films,single crystal Si was used as the substrate,a layer of anodic aluminum oxide(AAO)film was transferred on the Si su...To study the effect of different deposition temperatures on the optical properties of porous SiC films,single crystal Si was used as the substrate,a layer of anodic aluminum oxide(AAO)film was transferred on the Si substrate by chemical method,and then a layer of SiC was deposited on anodic aluminum oxide(AAO)template to prepare porous fluorescent SiC film by magnetron sputtering.The deposition temperature was ranged from 373 to 873 K.The thickness of the porous SiC film coated on the AAO surface was around 283 nm.It is found that the porous SiC with the deposition temperature of 873 K has the strongest photoluminescence(PL)intensity excited by 375 nm laser.The time-resolved PL spectra prove that the PL is mainly from intrinsic light emitting of SiC.With the optimized process,porous amorphous SiC film may have potential applications in the field of warm white LEDs.展开更多
Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scatter...Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scattering background,leading to great potential for in vivo imaging.Based on the limitations of the common spectral domain,and the superiority of the time-dimension,time-resolved imaging eliminates the auto-fuorescence in the biological tissue,thus supporting higher signal-to-noise ratio and sensitivities.The imaging technique is not affected by the difference in tissue composition or thickness and has the practical value of quan-titative in vivo detection.Almost all the relevant time-resolved imaging was carried out around lanthanide-doped upconversion nanomaterials,owing to the advantages of ultralong luminescence lifetime,excellent photostability,controllable morphology,easy surface modification and various strategies of regulating lifetime.Therefore,this review presents the research progress of SWIR time-resolved imaging technology based on nanomaterials doped with lanthanide ions as luminescence centers in recent years.展开更多
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
基金Project supported by the National Natural Science Foundation of China (20505020) the Natural Science Foundation ofGuangdong Province (06300086) +2 种基金 China Postdoctoral Science Foundation (20060390202) Scientific Research Fund ofHunan Provincial Education Department (05C508) Skeleton Youth Faculty Programof Hunan Higher Educational School
文摘The assembling of a coating of time-resolved fluorescent chelator BSPDA ( abbreviated for 4, 7-bis ( sulfhydrylphenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) onto a nano-gold layer was demonstrated. First, BSPDA was synthesized by simple procedures, and then an approach was developed to immobilize BSPDA onto the nano-gold layer deposited on a silane modified glass substrate, whereby europium ion (Ⅲ, Eu^3+ ) was captured and released owing to the interactive process of complexation and dissociation between BSPDA functionalized coating and Eu^3+ solution. The fluorescence spectra and related lifetimes were determined. Also, the BSPDA functionalized coating's specific complexation with Eu^3+ on the BSPDA assembly layer and the nonspecific adsorption of Eu^3+ on the nano-gold layer were compared. These results allowed a selective complexation of Eu^3+ by assembling a BSPDA chelating layer on the nano-gold layer; thus, a tunable time-resolved fluorescent layer was covalently attached, The results of the nanoparticle assembling and probing (or labeling) processes to specific bio-systems were very interesting and had significant implications to time-resolved-fluorescence-based detection on biosensor surfaces such as DNA chip and to arrayed light display devices.
基金This work was supported by Special Fund for Grain -scientific Research in the Public Interest (201513006-02), Special Fund for Agro -scientific Research in the Public Interest (201203094), Natural Science Foundation of China (31401601).
文摘Edible oil is one major nutritional ingredient to human and widely consumed directly. The contamination of aflatoxin B1 (AFB1) in edible oils has been attracted exten-sive efforts due to its hazard to human health and life. To avoid the digestion of edible oils contaminated by AFB1 the development of rapid and sensitive sensing method for AFB1 is required. Herein, a quantitative, sensitive and rapid method for AFB1 detection in edible oils was proposed by using ultrasensitive time-resolved fluorescent immunosensing (TRFIS) method. This method poses unique advantages from both time-resolved fluorescent sens-ing method and immunochromatographic assay format. The nanospheres were modified with fluorescent europium and then captured the home-made monoclonal antibody against AFB1 (3G1). After optimization, by using a competitive immunosensing manner, this TRFIS method has a detectable linear range of 0.54-20.0 μg/kg with minimum detectable concen-tration of 0.18μg/kg. It can be completed merely within 10 min with recovery from 87.0% to 121.9%. The agreement was observed between the results by TRFIS and high perfor-mance liquid chromatography (HPLC) methods. This research provides a promising sens-ing method for sensitive and rapid determining AFB1 in edible oils.
基金supported by grants from the International Science&Technology Cooperation Program of China(2009DFA32330)the Special Fund for Agro-scientific Research in the Public Interest(No.201203040)
文摘A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
文摘New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.
文摘N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals. With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers. The detection limit was calculated to be 40 ng/mL. Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.
基金supported by Natural Science Foundation of China(U1301214)Guangdong Natural Science Foundation(S2013030013338)+1 种基金the PhD Programs Foundation of Ministry of Education of China(20114404130002)National Department Public Benefit Research Foundation(201003008-08)
文摘Estrone has been identified as a potential endocrine-disrupting chemical (EDC)[1]. Estrone is usually quantified by gas chromatography-mass spectrometry (GC-MS), GC-MS/MS, high performance liquid chromatography (HPLC), HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay (ELISA) or chemiluminescence immunoassay (CLIA) for determination of estrone in real samples have been developed[2'4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods (with separation) to homogeneous assays (without separation)[5]. Fluorescence polarization immunoassay (FPIA) is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.
基金supported by National Commission of Natural Science Foundation of China.
文摘A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process is unnecessary.The recovery of HSA and albumin in urine is 107% and 95% respectively.The standard deviation is tess than 10%.
基金the National Natural Science Foundation of China(20490210)Shanghai Sci.Tech.Comm.(03QB14006,03DZ12031 and 05DJ14004)for financial support.
文摘A new europium complex is descried as a time-resolved luminescence-based sensor for fluoride anion. The sensor is selective even in the presence of intensive background fluorescence.
文摘Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS), China. However there has been less research activities in this field concerning the original process of the interaction between low-energy ions and complicated organisms. To address this challenge, an in situ multi-dimensional quantitative fluorescence microscopy system combined with the CAS-LIBB single-particle microbeam II endstation is proposed. In this article, the rationale, logistics and development of many aspects of the proposed system are discussed.
文摘The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μM and excitation energies above 1 mJ a long-lived, very intense emission, which appears within less than 5 ns and lasts up to 70 ns, is observed. During the first 50 ns the decay does not follow an exponential but rather a linear behaviour. In oxygen saturated solutions the long-lived emission is suppressed and solely short-lived fluorescence with τ 1-state and competes with the formation of DPH-O2 contact charge-transfer complexes and intersystem crossing which both quench the fluorescence. Our investigations show that even the small amount of oxygen dissolved in nitrogen saturated solutions has a distinct influence on the fluorescence kinetics of DPH.
基金the funding support from Key-Area Research and Development Program of Guangdong Province(No.2022B1111020003)Guangzhou Talents Program for Innovation and Entrepreneurship(No.2021-L010)the Foshan“Blue Ocean Talent Program”for Innovation and Entrepreneurship(No.2230032002063)。
文摘The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or Amplex red)is the most sensitive fluorogenic substrate known for HRP in current market,however,it suffers from some drawbacks,such as non-specific reactivity to carboxylesterase and limited fluorescence stability.In the present study,a novel HRP substrate10-cyclopropylcarbonyl-dichloro-dihydroxyphenoxazine(AR-2),has been prepared,which exhibited improved sensitivity than ADHP in sensing HRP.Moreover,the fluorescence of AR-2/HRP demonstrated improved tolerance to physiological relevant p H fluctuation as compared to ADHP/HRP.Successful detection of uric acid/urate oxidase reaction indicated excellent application prospect of AR-2/HRP for monitoring H_(2)O_(2)-generating biochemical reactions.More interestingly,an enzyme-linked immunosorbent assay(ELISA)using AR-2 as the fluorescence reporter has been successfully used in detecting IgG against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human serum samples.Overall,AR-2 exhibits improved performances over the commercial ADHP,which will be an ideal alternative to ADHP in HRP-based fluorescence biochemical analysis and immunoassays.
基金Funded by the National Natural Science Foundation of China(No.11747133)the Fundamental Research Funds for the Central Universities(No.195209019)。
文摘To study the effect of different deposition temperatures on the optical properties of porous SiC films,single crystal Si was used as the substrate,a layer of anodic aluminum oxide(AAO)film was transferred on the Si substrate by chemical method,and then a layer of SiC was deposited on anodic aluminum oxide(AAO)template to prepare porous fluorescent SiC film by magnetron sputtering.The deposition temperature was ranged from 373 to 873 K.The thickness of the porous SiC film coated on the AAO surface was around 283 nm.It is found that the porous SiC with the deposition temperature of 873 K has the strongest photoluminescence(PL)intensity excited by 375 nm laser.The time-resolved PL spectra prove that the PL is mainly from intrinsic light emitting of SiC.With the optimized process,porous amorphous SiC film may have potential applications in the field of warm white LEDs.
基金the National Natural Science Foundation of China(No.81971704)the National Key ResearchandDevelopment Program of China(No.2017YFA0205304)the Translational Medicine Research Fund of National Facility for Translational Medicine(Shanghai)(No.TMSK-2021-117)。
文摘Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scattering background,leading to great potential for in vivo imaging.Based on the limitations of the common spectral domain,and the superiority of the time-dimension,time-resolved imaging eliminates the auto-fuorescence in the biological tissue,thus supporting higher signal-to-noise ratio and sensitivities.The imaging technique is not affected by the difference in tissue composition or thickness and has the practical value of quan-titative in vivo detection.Almost all the relevant time-resolved imaging was carried out around lanthanide-doped upconversion nanomaterials,owing to the advantages of ultralong luminescence lifetime,excellent photostability,controllable morphology,easy surface modification and various strategies of regulating lifetime.Therefore,this review presents the research progress of SWIR time-resolved imaging technology based on nanomaterials doped with lanthanide ions as luminescence centers in recent years.