Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant species.The in...Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant species.The insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant species.Here,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated progeny.With PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this process.Using genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated plants.The results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.展开更多
Medicago truncatula is a chosen model for legumes towards deciphering fundamental legume biology,especially symbiotic nitrogen fixation.Current genomic resources for M.truncatula include a completed whole genome seque...Medicago truncatula is a chosen model for legumes towards deciphering fundamental legume biology,especially symbiotic nitrogen fixation.Current genomic resources for M.truncatula include a completed whole genome sequence information for R108 and Jemalong A17 accessions along with the sparse draft genome sequences for other 226 M.truncatula accessions.These genomic resources are complemented by the availability of mutant resources such as retrotransposon(Tnt1)insertion mutants in R108 and fast neutron bombardment(FNB)mutants in A17.In addition,several M.truncatula databases such as small secreted peptides(SSPs)database,transporter protein database,gene expression atlas,proteomic atlas,and metabolite atlas are available to the research community.This review describes these resources and provide information regarding how to access these resources.展开更多
基金supported by the National Natural Science Foundation of China(No.31471156)the Agri-X Project of Shanghai Jiao Tong University(Agri-X2015002)the Shanghai Graduate Education and Innovation Program(Horticulture)
文摘Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant species.The insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant species.Here,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated progeny.With PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this process.Using genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated plants.The results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.
基金supported by the National Science Foundation USA,Plant Genome Program grants (DBI 0703285,IOS-1127155,and IOS-1733470)in part by Noble Research Institute,LLC.
文摘Medicago truncatula is a chosen model for legumes towards deciphering fundamental legume biology,especially symbiotic nitrogen fixation.Current genomic resources for M.truncatula include a completed whole genome sequence information for R108 and Jemalong A17 accessions along with the sparse draft genome sequences for other 226 M.truncatula accessions.These genomic resources are complemented by the availability of mutant resources such as retrotransposon(Tnt1)insertion mutants in R108 and fast neutron bombardment(FNB)mutants in A17.In addition,several M.truncatula databases such as small secreted peptides(SSPs)database,transporter protein database,gene expression atlas,proteomic atlas,and metabolite atlas are available to the research community.This review describes these resources and provide information regarding how to access these resources.
