The present study aimed to investigate the targeting effect of H7K（R2）2-modified pH -sensitive liposomes on U87-MG cells. Using coumarin-6 as a fluorescence probe, we prepared H7K（R2）2-modified p H-sensitive lipos...The present study aimed to investigate the targeting effect of H7K（R2）2-modified pH -sensitive liposomes on U87-MG cells. Using coumarin-6 as a fluorescence probe, we prepared H7K（R2）2-modified p H-sensitive liposomes（designated as coumarin-6-PSL-H7K（R2）2）. The flow cytometry assay was used to evaluate the effect of H7K（R2）2 proportions on the cellular uptake and endocytosis pathways of coumarin--6--PSL--H7K（R2）2 on U87-MG cells. The circular dichroism（CD） spectroscopy assay was used to investigate the secondary structures of H7K（R2）2 peptide at pH 7.4 and H 6.8, respectively. Our results indicated that the 2.5% proportion of H7K（R2）2 in the coumarin-6--PSL-H7K（R2）2 was superior to those of 1% and 3.5% of H7K（R2）2. The uptake of coumarin--6-PSL--H7K（R2）2 on U87--MG cells was not inhibited by filipin, M-β--CD or chlorpromazine. The secondary structure of H7K（R2）2 at pH 6.8 was mostly presented as β--turn. In conclusion, we suggested that the appropriate proportion of H7K（R2）2 in the H7K（R2）2--modified pH--sensitive liposomes could be set at 2.5%. The cellular uptake pathway for H7K（R2）2-modified pH--sensitive liposomes was via the cell penetrating capacity of H7K（R2）2 which responded to acidic condition. The secondary structure of H7K（R2）2 at pH 6.8, which was presented as the shape of hairpin, might be mainly responsible for its targeting and cell penetrating effect.展开更多
Objective:To study the effects of Transient receptor potential cation channel,subfamily V,member 1(TRPV1) combined with lidocaine on status and apoptosis of U87-MG glioma cell line,and explore whether local anesthetic...Objective:To study the effects of Transient receptor potential cation channel,subfamily V,member 1(TRPV1) combined with lidocaine on status and apoptosis of U87-MG glioma cell line,and explore whether local anesthetic produces neurotoxicity by TRPVI.Methods:U87-MG cells were divided into control group,gene silencing group,empty vector group and TRPV gene up-rcgulation group.For cells in each group,flow cytometry was employed to detect the intracellular calcium ion concentration and mitochondrial membrane potential at different lime point from cellular perspective.Cell apoptosis of U87-MG was assayed by flow cytometry and MTT from a holistic perspective.Results:Calcium ion concentration increased along with time.The concentration in TRPV1 gene up-regulation group was significantly higher than those in other groups at each time point(P<0.05).After adding lidocaine.mitochondrial membrane potential in U87-MG significantly increased(P<0.05).This increasing trend in TRPV1 gene up-regulation group was more significant than other groups(P<0.05).while in TRPV1 gene silencing group,the trend significantly decreased(P<0.05).Flow cytometry result and MTT result both showed that cell apoptosis in each group significantly increased after lidocaine was added(P<0.05).This increasing trend in TRPV1 gene up-regulation group was more significant than other groups(P<0.05),while in TRPV1 gene silencing group,the trend significandy decreased(P<0.05).Moreover,apoptosis was more severe along with the increasing concentration of lidocaine(P<0.05).Conclusions:In this study,it was proved that lidocaine could dose-dependently induce the increase of intracellular calcium ion concentration,mitochondrial membrane potential and apoptosis in U87-MG glioma cell line.The up-regulation of TRPV1 enhanced cytotoxicity of lidocaine,which revealed the correlations between mem.Lidocaine might have increased intracellular calcium ion concentration by activating TRPVI gene and induced apoptosis of U87-GM glioma cell line by up-regulating mitochondrial membrane potential.展开更多
The expression of different vascular endothelial growth factor (VEGF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or g...The expression of different vascular endothelial growth factor (VEGF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or glucose deprivation conditions as model of ischemia. The blockade of function of the ERN1 enzyme, which is a major sensor of endoplasmic reticulum stress, leads to a decrease of the VEGFA, VEGFB and VEGFC mRNA expression level. The level of VEGFA proteins also decreases at this experimental condition in the cytosolic fraction, but increases in the nuclear fraction. Hypoxia does not affect VEGFC and increases the expression level of VEGFA and VEGFB mRNA in both used cell types, however, the change was much less profound in cells with suppressed function of ERN1. The expression level of VEGFC mRNA decreases in both used cell types in glutamine deprivation condition, however, the change was more profound in control glioma cells. At the same time, the expression level of VEGFA mRNA increases and VEGFB—decreases in gluta-mine deprivation condition in control glioma cells only. Exposure of glioma cells to glucose deprivation condition increases VEGFB mRNA expression level in both used cell types;however, VEGFA—in control glioma cells only and VEGFC—in cells with ERN1 signaling enzyme loss of function only. Thus, the results of this study clearly demonstrated the down-regulation of the expression of all three VEGF genes in glioma cells with ERN1 loss of function which correlates to the suppressed angiogenesis and proliferation rate of these cells. Moreover, the effect of hy-poxia and glutamine or glucose deprivation condition on the expression level of all VEGF genes is different and mainly depends on ERN1 signaling enzyme function.展开更多
We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was sho...We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.展开更多
目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保...目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保藏中心(Deutsche Sammlung von Mikroorganismen und Zellkulturen,DSMZ)数据库中U-87 MG]进行细胞生物学特性测定,对两株细胞用于后续研究工作的可能性进行评价。方法分别采用CCK-8法、划痕实验、Transwell实验等方法,并结合显微镜下细胞形态学特征,比较两株肿瘤细胞系的增生、迁移及侵袭能力等生物学特性。结果 (1)CCK-8法:细胞生长曲线提示细胞增生能力差异无统计学意义(P>0.05)。(2)划痕实验:两细胞系迁移能力差异无统计学意义(P>0.05)。(3)Transwell实验:两细胞系迁移能力差异无统计学意义(P>0.05),DSMZ数据库中U-87 MG细胞侵袭能力高于ATCC数据库中U-87 MG GlioblastomaAstrocytoma Human细胞,差异有统计学意义(P<0.05)。结论两株U87细胞系均可反映脑胶质瘤的生物学特性,可用于后续科研实验。两细胞系的增生、迁移能力差异无统计学意义,侵袭能力差异具有统计学意义,可根据实验目的选择合适的细胞系。展开更多
Objective:Glioma is a highly invasive tumor,frequently disposed in essential areas of the brain,which makes its surgical excision extremely difficult;meanwhile adjuvant therapy remains quite ineffective.Methods:In the...Objective:Glioma is a highly invasive tumor,frequently disposed in essential areas of the brain,which makes its surgical excision extremely difficult;meanwhile adjuvant therapy remains quite ineffective.Methods:In the current report,a new therapeutic approach in curing malignant neoplasms has been performed on the U87 human glioblastoma model.This approach,termed"Karanahan",is aimed at the eradication of cancer stem cells(CSCs),which were recently shown to be capable of internalizing fragments of extracellular double-stranded DNA.After being internalized,these fragments interfere in the process of repairing interstrand cross-links caused by exposure to appropriate cytostatics,and such an interference results either in elimination of CSCs or in the loss of their tumorigenic potency.Implementation of the approach requires a scheduled administration of cytostatic and complex composite double-stranded DNA preparation.Results:U87 cells treated in vitro in accordance with the Karanahan approach completely lost their tumorigenicity and produced no grafts upon intracerebral transplantation into immunodeficient mice.In SCID mice with developed subcutaneous grafts,the treatment resulted in reliable slowing down of tumor growth rate(P<0.05).In the experiment with intracerebral transplantation of U87 cells followed by surgical excision of the developed graft and subsequent therapeutic treatment,the Karanahan approach was shown to reliably slow down the tumor growth rate and increase the median survival of the mice twofold relative to the control.Conclusions:The effectiveness of the Karanahan approach has been demonstrated both in vitro and in vivo in treating developed subcutaneous grafts as well as orthotopic grafts after surgical excision of the tumor.展开更多
基金Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20120001110012)the National Basic Research Program of China(973 Program,Grant No.2013CB932501)National Natural Science Foundation of China(Grant No.81172992)
文摘The present study aimed to investigate the targeting effect of H7K（R2）2-modified pH -sensitive liposomes on U87-MG cells. Using coumarin-6 as a fluorescence probe, we prepared H7K（R2）2-modified p H-sensitive liposomes（designated as coumarin-6-PSL-H7K（R2）2）. The flow cytometry assay was used to evaluate the effect of H7K（R2）2 proportions on the cellular uptake and endocytosis pathways of coumarin--6--PSL--H7K（R2）2 on U87-MG cells. The circular dichroism（CD） spectroscopy assay was used to investigate the secondary structures of H7K（R2）2 peptide at pH 7.4 and H 6.8, respectively. Our results indicated that the 2.5% proportion of H7K（R2）2 in the coumarin-6--PSL-H7K（R2）2 was superior to those of 1% and 3.5% of H7K（R2）2. The uptake of coumarin--6-PSL--H7K（R2）2 on U87--MG cells was not inhibited by filipin, M-β--CD or chlorpromazine. The secondary structure of H7K（R2）2 at pH 6.8 was mostly presented as β--turn. In conclusion, we suggested that the appropriate proportion of H7K（R2）2 in the H7K（R2）2--modified pH--sensitive liposomes could be set at 2.5%. The cellular uptake pathway for H7K（R2）2-modified pH--sensitive liposomes was via the cell penetrating capacity of H7K（R2）2 which responded to acidic condition. The secondary structure of H7K（R2）2 at pH 6.8, which was presented as the shape of hairpin, might be mainly responsible for its targeting and cell penetrating effect.
基金supported by the National Natural Science Foundation(NO.81260176)
文摘Objective:To study the effects of Transient receptor potential cation channel,subfamily V,member 1(TRPV1) combined with lidocaine on status and apoptosis of U87-MG glioma cell line,and explore whether local anesthetic produces neurotoxicity by TRPVI.Methods:U87-MG cells were divided into control group,gene silencing group,empty vector group and TRPV gene up-rcgulation group.For cells in each group,flow cytometry was employed to detect the intracellular calcium ion concentration and mitochondrial membrane potential at different lime point from cellular perspective.Cell apoptosis of U87-MG was assayed by flow cytometry and MTT from a holistic perspective.Results:Calcium ion concentration increased along with time.The concentration in TRPV1 gene up-regulation group was significantly higher than those in other groups at each time point(P<0.05).After adding lidocaine.mitochondrial membrane potential in U87-MG significantly increased(P<0.05).This increasing trend in TRPV1 gene up-regulation group was more significant than other groups(P<0.05).while in TRPV1 gene silencing group,the trend significantly decreased(P<0.05).Flow cytometry result and MTT result both showed that cell apoptosis in each group significantly increased after lidocaine was added(P<0.05).This increasing trend in TRPV1 gene up-regulation group was more significant than other groups(P<0.05),while in TRPV1 gene silencing group,the trend significandy decreased(P<0.05).Moreover,apoptosis was more severe along with the increasing concentration of lidocaine(P<0.05).Conclusions:In this study,it was proved that lidocaine could dose-dependently induce the increase of intracellular calcium ion concentration,mitochondrial membrane potential and apoptosis in U87-MG glioma cell line.The up-regulation of TRPV1 enhanced cytotoxicity of lidocaine,which revealed the correlations between mem.Lidocaine might have increased intracellular calcium ion concentration by activating TRPVI gene and induced apoptosis of U87-GM glioma cell line by up-regulating mitochondrial membrane potential.
文摘The expression of different vascular endothelial growth factor (VEGF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or glucose deprivation conditions as model of ischemia. The blockade of function of the ERN1 enzyme, which is a major sensor of endoplasmic reticulum stress, leads to a decrease of the VEGFA, VEGFB and VEGFC mRNA expression level. The level of VEGFA proteins also decreases at this experimental condition in the cytosolic fraction, but increases in the nuclear fraction. Hypoxia does not affect VEGFC and increases the expression level of VEGFA and VEGFB mRNA in both used cell types, however, the change was much less profound in cells with suppressed function of ERN1. The expression level of VEGFC mRNA decreases in both used cell types in glutamine deprivation condition, however, the change was more profound in control glioma cells. At the same time, the expression level of VEGFA mRNA increases and VEGFB—decreases in gluta-mine deprivation condition in control glioma cells only. Exposure of glioma cells to glucose deprivation condition increases VEGFB mRNA expression level in both used cell types;however, VEGFA—in control glioma cells only and VEGFC—in cells with ERN1 signaling enzyme loss of function only. Thus, the results of this study clearly demonstrated the down-regulation of the expression of all three VEGF genes in glioma cells with ERN1 loss of function which correlates to the suppressed angiogenesis and proliferation rate of these cells. Moreover, the effect of hy-poxia and glutamine or glucose deprivation condition on the expression level of all VEGF genes is different and mainly depends on ERN1 signaling enzyme function.
文摘We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.
文摘目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保藏中心(Deutsche Sammlung von Mikroorganismen und Zellkulturen,DSMZ)数据库中U-87 MG]进行细胞生物学特性测定,对两株细胞用于后续研究工作的可能性进行评价。方法分别采用CCK-8法、划痕实验、Transwell实验等方法,并结合显微镜下细胞形态学特征,比较两株肿瘤细胞系的增生、迁移及侵袭能力等生物学特性。结果 (1)CCK-8法:细胞生长曲线提示细胞增生能力差异无统计学意义(P>0.05)。(2)划痕实验:两细胞系迁移能力差异无统计学意义(P>0.05)。(3)Transwell实验:两细胞系迁移能力差异无统计学意义(P>0.05),DSMZ数据库中U-87 MG细胞侵袭能力高于ATCC数据库中U-87 MG GlioblastomaAstrocytoma Human细胞,差异有统计学意义(P<0.05)。结论两株U87细胞系均可反映脑胶质瘤的生物学特性,可用于后续科研实验。两细胞系的增生、迁移能力差异无统计学意义,侵袭能力差异具有统计学意义,可根据实验目的选择合适的细胞系。
基金supported by the Russian Ministry of Science and High Education,State Budgeted Project 0259-2021-0013 for the Institute of Cytology and Genetics,Novosibirskthe Russian Foundation for Basic Research(Grant No.18-34-20016)+1 种基金Microscopic examination of bone marrow cells was supported by the Russian Ministry of Science and High EducationState Budgeted Project 0246-2021-0017 for the Institute of Molecular and Cellular Biology,Novosibirsk。
文摘Objective:Glioma is a highly invasive tumor,frequently disposed in essential areas of the brain,which makes its surgical excision extremely difficult;meanwhile adjuvant therapy remains quite ineffective.Methods:In the current report,a new therapeutic approach in curing malignant neoplasms has been performed on the U87 human glioblastoma model.This approach,termed"Karanahan",is aimed at the eradication of cancer stem cells(CSCs),which were recently shown to be capable of internalizing fragments of extracellular double-stranded DNA.After being internalized,these fragments interfere in the process of repairing interstrand cross-links caused by exposure to appropriate cytostatics,and such an interference results either in elimination of CSCs or in the loss of their tumorigenic potency.Implementation of the approach requires a scheduled administration of cytostatic and complex composite double-stranded DNA preparation.Results:U87 cells treated in vitro in accordance with the Karanahan approach completely lost their tumorigenicity and produced no grafts upon intracerebral transplantation into immunodeficient mice.In SCID mice with developed subcutaneous grafts,the treatment resulted in reliable slowing down of tumor growth rate(P<0.05).In the experiment with intracerebral transplantation of U87 cells followed by surgical excision of the developed graft and subsequent therapeutic treatment,the Karanahan approach was shown to reliably slow down the tumor growth rate and increase the median survival of the mice twofold relative to the control.Conclusions:The effectiveness of the Karanahan approach has been demonstrated both in vitro and in vivo in treating developed subcutaneous grafts as well as orthotopic grafts after surgical excision of the tumor.
