随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素...随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素对增强马铃薯耐UV-B辐射的分子机制,以马铃薯品种合作88为试验材料,对外源褪黑素处理UV-B辐射下的马铃薯叶片进行转录组测序,并进行基因本体(gene ontology, GO)功能聚类分析及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)功能聚类分析。结果表明,褪黑素可以促进马铃薯植株中缬氨酸、亮氨酸和异亮氨酸生物合成及精氨酸、脯氨酸代谢,提高内质网上蛋白质的加工水平,促进抗氧化物质编码基因的表达,激活UVR8等UV-B辐射的响应基因,从而缓解UV-B辐射对马铃薯植株的危害。综上所述,适量浓度的褪黑素对提高马铃薯耐UV-B辐射有积极作用,研究结果可为马铃薯抗UV-B辐射机制解析提供新的证据,并为其抗UV-B辐射研究提供一定的分子基础。展开更多
Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by appl...Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.展开更多
文摘随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素对增强马铃薯耐UV-B辐射的分子机制,以马铃薯品种合作88为试验材料,对外源褪黑素处理UV-B辐射下的马铃薯叶片进行转录组测序,并进行基因本体(gene ontology, GO)功能聚类分析及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)功能聚类分析。结果表明,褪黑素可以促进马铃薯植株中缬氨酸、亮氨酸和异亮氨酸生物合成及精氨酸、脯氨酸代谢,提高内质网上蛋白质的加工水平,促进抗氧化物质编码基因的表达,激活UVR8等UV-B辐射的响应基因,从而缓解UV-B辐射对马铃薯植株的危害。综上所述,适量浓度的褪黑素对提高马铃薯耐UV-B辐射有积极作用,研究结果可为马铃薯抗UV-B辐射机制解析提供新的证据,并为其抗UV-B辐射研究提供一定的分子基础。
基金supported by grants from the National Science Foundation of China (Grant Nos.32202466,32172593,and32272746)。
文摘Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.
