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载脂蛋白E敲除(ApoE^(-/-))小鼠整装(whole-mount)主动脉免疫荧光组织化学染色的血管外膜淋巴管成像
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作者 何玉莲 钟文飞 +3 位作者 林彩燕 李玉婷 冯森玲 严鹏科 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2024年第6期527-531,共5页
目的 探索载脂蛋白E敲除(ApoE^(-/-))小鼠整装(whole-mount)主动脉免疫荧光组织化学染色法显示血管外膜淋巴管的可行性。方法 制备ApoE^(-/-)小鼠整装主动脉,进行淋巴管内皮受体1(LYVE1)、 α平滑肌肌动蛋白(α-SMA)免疫荧光组织化学染... 目的 探索载脂蛋白E敲除(ApoE^(-/-))小鼠整装(whole-mount)主动脉免疫荧光组织化学染色法显示血管外膜淋巴管的可行性。方法 制备ApoE^(-/-)小鼠整装主动脉,进行淋巴管内皮受体1(LYVE1)、 α平滑肌肌动蛋白(α-SMA)免疫荧光组织化学染色,染色后组织经5 g/L苏丹黑B处理30 min、组织透明液透明,移至自制的样本容器中,荧光显微镜下成像。结果经5 g/L苏丹黑B处理的整装主动脉可见血管自发荧光显著降低,外膜淋巴管的特异性荧光强度显著增强,更易观察且不会对其他通道荧光产生干扰。结论 建立的ApoE^(-/-)小鼠整装主动脉免疫荧光组织化学染色显示血管外膜淋巴管的方法操作简便,特异性好。 展开更多
关键词 载脂蛋白E敲除(ApoE^(-/-)) 整装(whole-mount)小鼠主动脉 动脉粥样硬化 淋巴管 苏丹黑B
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To Analyze the Sensitivity of RT-PCR Assays Employing S Gene Target Failure with Whole Genome Sequencing Data during Third Wave by SARS-CoV-2 Omicron Variant
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作者 Pooja Patel Yogita Mistry +1 位作者 Monika Patel Summaiya Mullan 《Advances in Microbiology》 CAS 2024年第5期247-255,共9页
Introduction: Omicron is a highly divergent variant of concern (VOCs) of a severe acute respiratory syndrome SARS-CoV-2. It carries a high number of mutations in its spike protein hence;it is more transmissible in the... Introduction: Omicron is a highly divergent variant of concern (VOCs) of a severe acute respiratory syndrome SARS-CoV-2. It carries a high number of mutations in its spike protein hence;it is more transmissible in the community by immune evasion mechanisms. Due to mutation within S gene, most Omicron variants have reported S gene target failure (SGTF) with some commercially available PCR kits. Such diagnostic features can be used as markers to screen Omicron. However, Whole Genome Sequencing (WGS) is the only gold standard approach to confirm novel microorganisms at genetically level as similar mutations can also be found in other variants that are circulating at low frequencies worldwide. This Retrospective study is aimed to assess RT-PCR sensitivity in the detection of S gene target failure in comparison with whole genome sequencing to detect variants of Omicron. Methods: We have analysed retrospective data of SARS-CoV-2 positive RT-PCR samples for S gene target failure (SGTF) with TaqPath COVID-19 RT-PCR Combo Kit (ThermoFisher) and combined with sequencing technologies to study the emerged pattern of SARS-CoV-2 variants during third wave at the tertiary care centre, Surat. Results: From the first day of December 2021 till the end of February 2022, a total of 321,803 diagnostic RT-PCR tests for SARS-CoV-2 were performed, of which 20,566 positive cases were reported at our tertiary care centre with an average cumulative positivity of 6.39% over a period of three months. In the month of December 21 samples characterized by the SGTF (70/129) were suggestive of being infected by the Omicron variant and identified as Omicron (B.1.1.529 lineage) when sequence. In the month of January, we analysed a subset of samples (n = 618) with SGTF (24%) and without SGTF (76%) with Ct values Conclusions: During the COVID-19 pandemic, it took almost more than 15 days to diagnose infection and identify pathogen by sequencing technology. In contrast to that molecular assay provided quick identification with the help of SGTF phenomenon within 5 hours of duration. This strategy helps scientists and health policymakers for the quick isolation and identification of clusters. That ultimately results in a decreased transmission of pathogen among the community. 展开更多
关键词 SARS-CoV-2 S Gene Target Failure whole Genome Sequencing Omicron
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Assessment of BRRI Whole Feed Combine Harvester (Model BRRI WCH2021) for Mechanized Rice Harvesting in Bangladesh
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作者 Arafat Ullah Khan Akm Saiful Islam +5 位作者 Md. Kamruzzaman Pintu Subrata Paul Md. Durrul Huda Md. Anwar Hossen Md. Monirul Islam Md. Moudud Ahmmed 《Agricultural Sciences》 2024年第2期274-291,共18页
Agricultural mechanization plays a pivotal role in the transition from subsistence to commercial agriculture, with a particular focus on labour-intensive activities like harvesting. This study assesses the operational... Agricultural mechanization plays a pivotal role in the transition from subsistence to commercial agriculture, with a particular focus on labour-intensive activities like harvesting. This study assesses the operational characteristics of the BRRI Whole Feed Combine Harvester (Model BRRI WCH2021) at the field level. Developed under the SFMRA project, the harvester’s technical performance and loss assessment were conducted during the Boro 2022 and Aman 2022 seasons in farmer fields in Bangladesh’s Rangpur region. The field efficiency of the harvester was determined to be 62.5% and 57.9% in the Boro and Aman seasons, respectively. Fuel consumption rates were recorded at 2.77 l/ha and 2.31 l/ha for the Boro and Aman seasons. The total harvesting losses, encompassing cutter bar, shatter, cylinder, and separation loss, averaged 0.56% and 0.48% in the Boro and Aman seasons, respectively. Mechanized harvesting with the BRRI Whole Feed Combine Harvester significantly reduced paddy losses by 5.81% compared to manual methods. The field evaluation results indicate the combine harvester’s satisfactory performance, highlighting its potential to alleviate labour demands during peak harvesting. The development of the BRRI WCH offers a sustainable solution for rice harvesting mechanization among progressive farmers. It paves the way for the broader adoption of advanced agricultural technology in Bangladesh. 展开更多
关键词 BRRI whole Feed Combine Harvester Field Efficiency Fuel Consumption Harvesting Loss
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Whole Perfect Vectors and Fermat’s Last Theorem
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作者 Ramon Carbó-Dorca 《Journal of Applied Mathematics and Physics》 2024年第1期34-42,共9页
A naïve discussion of Fermat’s last theorem conundrum is described. The present theorem’s proof is grounded on the well-known properties of sums of powers of the sine and cosine functions, the Minkowski norm de... A naïve discussion of Fermat’s last theorem conundrum is described. The present theorem’s proof is grounded on the well-known properties of sums of powers of the sine and cosine functions, the Minkowski norm definition, and some vector-specific structures. 展开更多
关键词 Fermat’s Last Theorem whole Perfect Vectors Sine and Cosine Functions Natural and Rational Vectors Fermat Vectors
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Haematology Parameters of Apparently Healthy Prospective Whole Blood Donors in a Nigerian Hospital Setting
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作者 Taiwo Modupe Balogun Kingsley Aile +2 位作者 Athanasius Chika Nnamani Olayinka Saidat Kareem Adenekan Salu 《Open Journal of Blood Diseases》 2023年第2期59-68,共10页
Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently... Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently healthy prospective whole blood donors. Participants and Methods: This was a hospital based prospective study carried out from August to October 2020 at the blood transfusion unit of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Nigeria. A structured pretested questionnaire was used for data collection. The socio demographic status and the haematology parameters of apparently healthy prospective whole blood donors who tested negative for HIV, hepatitis B and C markers were captured. Obtained data were analysed with the statistical package for the social scientist software version 20. Results: One hundred male (97.1%) and three female (2.9%) apparently healthy prospective whole blood donors were studied. The median age of study subjects was 30 years. Obtained median haematology parameter values were 13 g/dl, 40%, 4.9/nl and 203.9/nl for haemoglobin concentration, haematocrit, total white cell and platelet counts respectively. The median values for the mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) of participants were 32.6 g/dl, 27.7 pg and 85.7 fl respectively. Observed prevalence of subnormal haematology parameters for haemoglobin concentration, total white cells, platelets were 12.6%, 25.2%, and 13.6% respectively. Also subnormal values for MCHC, MCH, MCV were 11.7%, 26.2%, and 16.5% respectively among prospective whole blood donors in this study. No higher than normal haematology parameter values were observed. Median values for erythrocyte sedimentation rate was 8.4 mm/hr. Conclusion: A significant percentage of apparently healthy prospective whole blood donors had subnormal haematology parameters values. Obtained normal values in our study are comparable with local reference range reports from previous studies in Nigeria and other parts of Africa. 124947 . 展开更多
关键词 whole Blood Donors Selection Haematology Parameters CD4 +ve T Lymphocyte Counts Nigeria
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Part-Whole关系的细分及性质分析 被引量:2
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作者 连莉 马军 +1 位作者 雷景生 宋玲 《计算机工程》 EI CAS CSCD 北大核心 2006年第17期83-85,共3页
Part-Whole关系的语义差别往往决定了本体是否可以合并或重用。Wiston分类法被用来对医学本体中的Part-Whole关系进行了分类,使每类Part-Whole子关系具有特定的语义并有传递性。利用Mereology理论分析了子关系的性质和子关系之间的相似... Part-Whole关系的语义差别往往决定了本体是否可以合并或重用。Wiston分类法被用来对医学本体中的Part-Whole关系进行了分类,使每类Part-Whole子关系具有特定的语义并有传递性。利用Mereology理论分析了子关系的性质和子关系之间的相似性,以及子关系之间相似性和逻辑推理正确性的关系。为Part-Whole关系的研究提供了新的方法学。 展开更多
关键词 本体 Part-whole关系 语义WEB
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蒙特卡罗法在化感作用全区间抑制强度评估(wholerange assessment)中的应用
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作者 刘迎湖 付银莲 +2 位作者 陈小秋 安民 陈实 《生态科学》 CSCD 2009年第4期357-361,共5页
植物化感作用的强弱与化感物质的作用浓度相关。化感作用全区间抑制强度指数(Whole-range assessmentindex)是用于评估植物在一定的化感物质作用范围内所受化感作用总体抑制程度的一种方法。在对非线性剂量响应进行曲线拟合的基础上,本... 植物化感作用的强弱与化感物质的作用浓度相关。化感作用全区间抑制强度指数(Whole-range assessmentindex)是用于评估植物在一定的化感物质作用范围内所受化感作用总体抑制程度的一种方法。在对非线性剂量响应进行曲线拟合的基础上,本研究应用蒙特卡罗模拟法为计算化感作用全区间抑制强度指数提供了一种科学的数学计算方法,并应用该计算方法分析了番茄水浸提液对生菜、萝卜、白菜、菜心、包心菜、豆角和水稻的化感作用强度。 展开更多
关键词 化感作用 低促高抑(赫米斯Hormesis) 化感作用全区间抑制强度指数(whole-range assessment index) An.Johnson.Lover Hormesis模型 蒙特卡罗模拟法(Monte Carlo simulation)
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Whole Person Education of English Majors through English Public Speaking
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作者 王玉明 王晨 +2 位作者 吴闻博 陈文娟 何倩倩 《海外英语》 2015年第4期125-127,共3页
English public speaking proves to play a significant role in the speaker's whole person education, which has been gaining increasing attention among scholars at home and abroad. The paper analyzes possible relatio... English public speaking proves to play a significant role in the speaker's whole person education, which has been gaining increasing attention among scholars at home and abroad. The paper analyzes possible relations between them and argues that great importance and awareness are supposed to be attached to the development and promotion of English public speaking especially among English majors for them to be more versatile and more competitive both in job markets and in work places. 展开更多
关键词 whole PERSON EDUCATION ENGLISH majors ENGLISH public SPEAKING
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Molecular diagnosis of autosomal recessive cerebellar ataxia in the whole exome/genome sequencing era
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作者 Christina Votsi Kyproula Christodoulou 《World Journal of Neurology》 2013年第4期115-128,共14页
Autosomal recessive cerebellar ataxias(ARCA) are a clinically and genetically heterogeneous group of rare neurodegenerative disorders characterized by autosomal recessive inheritance and an early age of onset. Progres... Autosomal recessive cerebellar ataxias(ARCA) are a clinically and genetically heterogeneous group of rare neurodegenerative disorders characterized by autosomal recessive inheritance and an early age of onset. Progressive ataxia is usually the prominent symptom and is often associated with other neurological or additional features. ARCA classification still remains controversial even though different approaches have been proposed over the years. Furthermore, ARCA molecular diagnosis has been a challenge due to phenotypic overlap and increased genetic heterogeneity observed within this group of disorders. Friedreich's ataxia and ataxia telangiectasia have been reported as the most frequent and well-studied forms of ARCA. Significant progress in understanding the genetic etiologies of the ARCA has been achieved during the last 15 years. The methodological revolution that has been observed in genetics over the last few years has contributed significantly to the molecular diagnosis of rare diseases including the ARCAs. Development of high throughput technologies has resulted in the identification of new ARCA genes and novel mutations in known ARCA genes. Therefore,an improvement in the molecular diagnosis of ARCA is expected. Moreover, based on the fact that many patients still remain undiagnosed, additional forms of ataxia are expected to be identified. We hereby review the current knowledge on the ARCAs, focused on the genetic findings of the most common forms that were molecularly characterized before the whole exome/genome era, as well as the most recently described forms that have been elucidated with the use of these novel technologies. The significant contribution of wholeexome sequencing or whole-genome sequencing in the molecular diagnosis of ARCAs is discussed. 展开更多
关键词 Autosomal RECESSIVE cerebellar ATAXIA whole-exome SEQUENCING whole-GENOME SEQUENCING HOMOZYGOSITY mapping Next generation SEQUENCING
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INTELLIGENT VIRTUAL CONTROL:MEASURING INSTRUMENT FROM WHOLE TO PART 被引量:19
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作者 Qin Shuren Tang BaopingCollege of Mechanical Engineering, Chongqing University, Chongqing 400044, China 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2002年第2期131-135,共5页
A new concept called intelligent virtual control (IVC), which can be drivenby measuring functions, is put forward. This small 'intelligent measurement instrument unit (IMIU)',carrying with functions of instrum... A new concept called intelligent virtual control (IVC), which can be drivenby measuring functions, is put forward. This small 'intelligent measurement instrument unit (IMIU)',carrying with functions of instrument, consists of different types of intelligent virtualinstrument (IVI) through individual components together as building blocks and can be displayeddirectly on the computer screen. This is a new concept of measuring instrument, and also animportant breakthrough after virtual instrument (VI). Virtual control makes instrument resourcesobtain further exploitation. It brings about a fundamental change to the design and manufacturingmode. The instrument therefore, can not only be produced directly inside a PC, but the product isinvolved in the 'green product' system. So far, all the present digital instruments will grow to bereplaced by intelligent control with green characteristics. 展开更多
关键词 Intelligent virtual control Measurement instrument whole Part
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基于Part-Whole关系的人造物属性分析 被引量:1
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作者 雷玉霞 眭跃飞 曹宝香 《计算机工程与应用》 CSCD 北大核心 2004年第3期63-64,86,共3页
知识分析是建立知识库系统过程中的重要一环,其目的就是帮助检查知识库中的知识一致性、完备性和精确性。该文重点讨论了基于Part-Whole关系的人造物属性分析,主要内容是:一是部分和整体的属性之间是否具有继承关系;二是部分的公共属性... 知识分析是建立知识库系统过程中的重要一环,其目的就是帮助检查知识库中的知识一致性、完备性和精确性。该文重点讨论了基于Part-Whole关系的人造物属性分析,主要内容是:一是部分和整体的属性之间是否具有继承关系;二是部分的公共属性和整体相同属性的属性值之间的内在约束关系。 展开更多
关键词 知识表示 知识分析 Part—Whok关系 人造物 知识库系统 属性分析
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Whole-Process Pollution Control for Cost-Effective and Cleaner Chemical Production A Case Study of the Tungsten Industry in China 被引量:9
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作者 Hongbin Cao He Zhao +7 位作者 Di Zhang Chenming Liu Xiao Lin Yuping Li Pengge Ning Jiajun Sun Yi Zhang Zhi Sun 《Engineering》 SCIE EI 2019年第4期768-776,共9页
In this research,a methodology named whole-process pollution control(WPPC)is demonstrated that improves the effectiveness of process optimization.This methodology considers waste/emission treatment as a step of the wh... In this research,a methodology named whole-process pollution control(WPPC)is demonstrated that improves the effectiveness of process optimization.This methodology considers waste/emission treatment as a step of the whole production process with respect to the minimization of cost and environmental impact for the whole process.The following procedures are introduced in a WPPC process optimization:①a material and energy flow investigation and optimization based on a systematic understanding of the distribution and physiochemical properties of potential pollutants;②a process optimization to increase the utilization efficiency of different elements and minimize pollutant emissions;and③an evaluation to reveal the effectiveness of the optimization strategies.The production of ammonium paratungstate was chosen for the case study.Two factors of the different optimization schemes-namely the cost-effectiveness factor and the environmental impact indicator-were evaluated and compared.This research demonstrates that by considering the nature of potential pollutants,technological innovations,economic viability,environmental impacts,and regulation requirements,WPPC can efficiently optimize a metal production process. 展开更多
关键词 whole-process POLLUTION control PROCESS optimization Industrial POLLUTION Tungsten
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HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays 被引量:13
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作者 Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome +4 位作者 Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto 《World Journal of Gastroenterology》 SCIE CAS 2013年第38期6438-6446,共9页
AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We... AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. 展开更多
关键词 Gastric cancer Human epidermalgrowth factor receptor 2 IMMUNOHISTOCHEMISTRY whole-tissue SECTIONS TISSUE MICROARRAY TRASTUZUMAB
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Whole-exome sequencing identifies novel mutations in genes responsible for retinitis pigmentosa in 2 nonconsanguineous Chinese families 被引量:7
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作者 Yan-Shan Hu Hui Song +2 位作者 Yin Li Zi-Yun Xiao Tuo Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2019年第6期915-923,共9页
AIM: To detect the pathogenetic mutations responsible for nonsyndromic autosomal recessive retinitis pigmentosa(RP) in 2 nonconsanguineous Chinese families. METHODS: The clinical data, including detailed medical histo... AIM: To detect the pathogenetic mutations responsible for nonsyndromic autosomal recessive retinitis pigmentosa(RP) in 2 nonconsanguineous Chinese families. METHODS: The clinical data, including detailed medical history, best corrected visual acuity(BCVA), slit-lamp biomicroscope examination, fundus photography, optical coherence tomography, static perimetry, and full field electroretinogram, were collected from the members of 2 nonconsanguineous Chinese families preliminarily diagnosed with RP. Genomic DNA was extracted from the probands and other available family members;wholeexome sequencing was conducted with the DNA samples provided by the probands, and all mutations detected by whole-exome sequencing were verified using Sanger sequencing in the probands and the other available family members. The verified novel mutations were further sequenced in 192 ethnicity matched healthy controls.RESULTS: The patients from the 2 families exhibited the typical symptoms of RP, including night blindness and progressive constriction of the visual field, and the fundus examinations showed attenuated retinal arterioles, peripheral bone spicule pigment deposits, and waxy optic discs. Whole-exome sequencing revealed a novel nonsense mutation in FAM161 A(c.943 A>T, p.Lys315*) and compound heterozygous mutations in RP1 L1(c.56 C>A, p.Pro19 His;c.5470 C>T, p.Gln1824*). The nonsense c.5470 C>T, p.Gln1824* mutation was novel. All mutations were verified by Sanger sequencing. The mutation p.Lys315* in FAM161A co-segregated with the phenotype, and all the nonsense mutations were absent from the ethnicity matched healthy controls and all available databases.CONCLUSION: We identify 2 novel mutations in genes responsible for autosomal recessive RP, and the mutation in FAM161A is reported for the first time in a Chinese population. Our result not only enriches the knowledge of the mutation frequency and spectrum in the genes responsible for nonsyndromic RP but also provides a new target for future gene therapy. 展开更多
关键词 RETINITIS pigmentosa NONSYNDROMIC whole-exome SEQUENCING MUTATION novel
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Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice(Oryza sativa L.) 被引量:8
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作者 CHAI Lu ZHANG Jian +7 位作者 PAN Xiao-biao ZHANG Fan ZHENG Tian-qing ZHAO Xiu-qing WANG Wen-sheng Ali Jauhar XU Jian-long LI Zhi-kang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第8期1609-1620,共12页
Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance(ST) was genetically dissected by phenotypin... Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance(ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines(ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL(AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and nonstress conditions were identified, most(〉90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan(HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection(MAS) or genome selection. 展开更多
关键词 RICE whole plant growth duration salt tolerance quantitative trait loci(QTLs) EPISTASIS
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From leaf to whole-plant water use efficiency(WUE)in complex canopies:Limitations of leaf WUE as a selection target 被引量:10
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作者 Hipólito Medrano Magdalena Tomás +6 位作者 Sebastià Martorell aume Flexas Esther Hernández Joan Rosselló Alicia Pou José-Mariano Escalona Josefina Bota 《The Crop Journal》 SCIE CAS CSCD 2015年第3期220-228,共9页
Plant water use efficiency(WUE) is becoming a key issue in semiarid areas, where crop production relies on the use of large volumes of water. Improving WUE is necessary for securing environmental sustainability of foo... Plant water use efficiency(WUE) is becoming a key issue in semiarid areas, where crop production relies on the use of large volumes of water. Improving WUE is necessary for securing environmental sustainability of food production in these areas. Given that climate change predictions include increases in temperature and drought in semiarid regions,improving crop WUE is mandatory for global food production. WUE is commonly measured at the leaf level, because portable equipment for measuring leaf gas exchange rates facilitates the simultaneous measurement of photosynthesis and transpiration. However,when those measurements are compared with daily integrals or whole-plant estimates of WUE, the two sometimes do not agree. Scaling up from single-leaf to whole-plant WUE was tested in grapevines in different experiments by comparison of daily integrals of instantaneous water use efficiency [ratio between CO2assimilation(AN) and transpiration(E); AN/E] with midday AN/E measurements, showing a low correlation, being worse with increasing water stress. We sought to evaluate the importance of spatial and temporal variation in carbon and water balances at the leaf and plant levels. The leaf position(governing average light interception) in the canopy showed a marked effect on instantaneous and daily integrals of leaf WUE. Night transpiration and respiration rates were also evaluated, as well as respiration contributions to total carbon balance. Two main components were identified as filling the gap between leaf and whole plant WUE: the large effect of leaf position on daily carbon gain and water loss and the large flux of carbon losses by dark respiration. These results show that WUE evaluation among genotypes or treatments needs to be revised. 展开更多
关键词 WATER USE Drought Intrinsic WATER USE EFFICIENCY 13C Instantaneous WATER USE EFFICIENCY whole plant WATER USE EFFICIENCY
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Whole-body heat exposure induces membrane changes in spermatozoa from the cauda epididymidis of laboratory mice 被引量:4
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作者 Harsha Wechalekar Brian P. Setchell +3 位作者 Eleanor J. Peirce Mario Ricci Chris Leigh William G. Breed 《Asian Journal of Andrology》 SCIE CAS CSCD 2010年第4期591-598,共8页
This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of lab... This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of laboratory mice was investigated. C57BL/6 mice (n = 7) were housed in a microclimate chamber at 37℃-38℃ for 8 h per day for three consecutive days, while control mice (n = 7) were kept at 23℃-24℃. Cauda epididymal spermatozoa were obtained 16 h after the last heat treatment. The results showed that sperm numbers were similar in the two groups (P = 0.23), but after heat treatment, a significant reduction in the percentage of motile sperm was present (P 〈 0.0001). Membrane changes of the spermatozoa were investigated by staining with phycoerythrin (PE)- conjugated Annexin V, which detects exteriorization of phosphotidylserine from the inner to the outer leaflet of the sperm plasma membrane, and 7-aminoactinomycin D (7-AAD), which binds to the sperm nucleus when the plasma membrane is damaged. The percentage of spermatozoa showing positive staining with Annexin V-PE or 7-AAD or both, was significantly higher (P 〈 0.05) in heat-exposed mice compared with controls. These results show that whole-body heat exposure to 37℃-38℃ induces membrane changes in the epididymal spermatozoa of mice, which may lead to apoptosis. 展开更多
关键词 laboratory mice sperm membrane damage whole-body heat
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A Whole Plant Herbaceous Angiosperm from the Middle Jurassic of China 被引量:2
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作者 HAN Gang LIU Zhongjian +3 位作者 LIU Xueling MAO Limi Frederic M.B. JACQUES WANG Xin 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2016年第1期19-29,共11页
In contrast to woody habit with secondary growth, truthful herbaceous habit lacking secondary growth is restricted to angiosperms among seed plants. Although angiosperms might have occurred as early as in the Triassic... In contrast to woody habit with secondary growth, truthful herbaceous habit lacking secondary growth is restricted to angiosperms among seed plants. Although angiosperms might have occurred as early as in the Triassic and herbaceous habit theoretically may have been well adopted by pioneer angiosperms, pre-Cretaceous herbs are missing hitherto, leaving the origin of herbs and evolution of herbaceous angiosperms mysterious. Here we report Juraherba bodae gen. et sp. nov, a whole plant herbaceous angiosperm, from the Middle Jurassic (〉164 Ma) at Daohugou Village, Inner Mongolia, China, a fossil Lagerst^itten that is worldwide famous for various fossil finds. The angiospermous affinity of Juraherba is ensured by its enclosed ovules/seeds. The plant is small but complete, with physically connected hairy root, stem, leaves, and fructifications. The Middle Jurassic age recommends Juraherba as the earliest record of herbaceous seed plants, demanding a refresh look at the evolutionary history of angiosperms. 展开更多
关键词 seed plant JURASSIC HERBACEOUS whole plant angiosperm.
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Successful multidisciplinary clinical approach and molecular characterization by whole transcriptome sequencing of a cardiac myxofibrosarcoma: A case report 被引量:3
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作者 Maristella Saponara Valentina Indio +16 位作者 Carmine Pizzi Elena-Daniela Serban Milena Urbini Annalisa Astolfi Pasquale Paolisso Sofia Martin Suarez Margherita Nannini Davide Pacini Valentina Agostini Ornella Leone Valentina Ambrosini Giuseppe Tarantino Stefano Fanti Fabio Niro Francesco Buia Domenico Attinà Maria Abbondanza Pantaleo 《World Journal of Clinical Cases》 SCIE 2019年第19期3018-3026,共9页
BACKGROUND Cardiac tumors are rare and complex entities.Surgery represents the cornerstone of therapy,while the role of adjuvant treatment remains unclear and,in case of relapse or metastatic disease,the prognosis is ... BACKGROUND Cardiac tumors are rare and complex entities.Surgery represents the cornerstone of therapy,while the role of adjuvant treatment remains unclear and,in case of relapse or metastatic disease,the prognosis is very poor.Lack of prospective,randomized clinical trials hinders the generation of high level evidence for the optimal diagnostic workup and multimodal treatment of cardiac sarcomas.Herein,we describe the multidisciplinary clinical management and molecular characterization of a rare case of cardiac myxofibrosarcoma in an elderly woman.CASE SUMMARY A 73-year-old woman presented signs and symptoms of acute left-sided heart failure.Imaging examination revealed a large,left atrial mass.With suspicion of a myxoma,she underwent surgery,and symptoms were promptly relieved.Histology showed a cardiac myxofibrosarcoma,a rare histotype of cardiac sarcoma.Eight months later,disease unfortunately relapsed,and after a multidisciplinary discussion,a chemotherapy with doxorubicin and then gemcitabine was started,achieving partial radiologic and complete metabolic response,which was maintained up to 2 years and is still present.This report is focused on the entire clinical path of our patient from diagnosis to follow-up,through surgery and strategies adopted at relapse.Moreover,due to their rarity,very little is known about the molecular landscape of myxofibrosarcomas.Thus,we also performed and described preliminary genome analysis of the tumor tissue to get further insight on mechanisms involved in tumor growth,and to possibly unveil new clinically actionable targets.CONCLUSION We report a case of cardiac myxofibrosarcoma that achieved a very good prognosis due to an integrated surgical,cardiac and oncologic treatment strategy. 展开更多
关键词 CARDIAC SARCOMA MYXOFIBROSARCOMA whole transcriptome sequencing Doxorubicin GEMCITABINE Case report
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Identification of rare paired box 3 variant in strabismus by whole exome sequencing 被引量:3
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作者 Hui-Min Gong Jing Wang +3 位作者 Jing Xu Zhan- Yu Zhou Jing- Wen Li Shu-Fang Chen 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第8期1223-1228,共6页
AIM: To identify the potentially pathogenic gene variants that contributes to the etiology of strabismus. METHODS: A Chinese pedigree with strabismus was collected and the exomes of two affected individuals were se... AIM: To identify the potentially pathogenic gene variants that contributes to the etiology of strabismus. METHODS: A Chinese pedigree with strabismus was collected and the exomes of two affected individuals were sequenced using the next-generation sequencing technology. The resulting variants from exome sequencing were filtered by subsequent bioinformatics methods and the candidate mutation was verified as heterozygous in the affected proposita and her mother by sanger sequencing. RESULTS: Whole exome sequencing and filtering identified a nonsynonymous mutation Co434G-T transition in paired box 3 (PAX3) in the two affected individuals, which were predicted to be deleterious by more than 4 bioinformatics programs. This altered amino acid residue was located in the conserved PAX domain of PAX3. This gene encodes a member of the PAX family of transcription factors, which play critical roles during fetal development. Mutations in PAX3 were associated with Waardenburg syndrome with strabismus. CONCLUSION: Our results report that the c.434G-T mutation (p.R145L) in PAX3 may contribute to strabismus, expanding our understanding of the causally relevant genes for this disorder. 展开更多
关键词 STRABISMUS whole exome sequencing paired box 3
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