To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,...To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,we predicted the target proteins associated with these active ingredients.In parallel,we employed the GeneCards database to identify genes related to PCOS and determined the intersection of drug targets and disease targets using the online tool available at http://bioinformatics.psb.ugent.be/webtools/Venn/.The shared genes were considered as the target proteins of Wuji powder in the treatment of PCOS.Utilizing the String website,we constructed a protein-protein interaction(PPI)network diagram and identified key protein modules and hub genes within the PPI network using Cytoscape 3.7.1 software.Further analysis in the DAVID database involved GO and KEGG pathway enrichment analyses of the genes within the identified key modules.Our investigation revealed a total of 63 active ingredients in Wuji powder with potential therapeutic effects on PCOS,corresponding to 266 drug targets.Intersection with PCOS-related disease targets yielded 174 shared targets.Ten key modules and ten hub genes(TNF,MMP9,AKT1,ECG,VEGFA,PTGS2,IL-6,MAPK3,STAT3,and CXCL8)were identified through network analysis.KEGG pathway enrichment analysis uncovered 58 signaling pathways,including TNF,MAPK,and PI3K-Akt signaling pathways.GO functional annotation delineated five cellular components(CC),nine molecular functions(MF),and 58 biological processes(BP).Noteworthy findings included extracellular space,enzyme binding,and drug response among CC,MF,and BP categories,respectively.These results collectively suggested that Wuji powder might exert its therapeutic effects on PCOS by modulating the TNF/PI3K/AKT signaling pathway.展开更多
Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was a...Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was an important commercial town and a political,economic and cultural center in the lower and middle reaches of Mishui River due to the particular historical opportunities.Its tourist resources are characterized by "integrated natural scenery and ancient town,highly concentrated scenic spots,prosperous business in the history,row upon row of stores and workshops,numerous temples and ancestral halls,and various festivals".It was proposed that the protection and rural tourism development of Wuji Town should be based on consciousness raising,unification of concepts,scientific planning,application of integrated protection means,and development of regional clusters on the basis of famous mountain and city.展开更多
Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Ch...Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Chinese Pharmacopoeia(2020 Edition)and related literature were searched to identify the main chemical composition and targets of constituent drugs in Wuji pill,including Houpo(Magnoliae Officinalis Cortex),Huangqin(Scutellariae Radix),Badou(Crotonis Fructus),Huanglian(Coptidis Rhizoma)and Renshen(Ginseng Radix et Rhizoma).Meanwhile,Genecards,OMIM,TTD,Drugbank and PharmGkb databases were used to obtain the main targets of LC.The"chemical compound-target"network was constructed by using Cytoscape 3.8.2 software with the disease drug intersection gene as the research target.The PPI network was constructed by using the string database for protein interaction analysis,and the core target genes were sorted out.The Gene ontology go function enrichment analysis and KEGG signal pathway enrichment analysis were carried out based on the ClusterProfiler of R software.Finally,the Autodock Vina software was used to conduct molecular docking between the core target with high value and the key components.Results:A total of 60 active components,190 corresponding targets and 2516 GO and 163 KEGG pathways of Wuji pill were screened out,including 179 potential targets of LC.The core targets were ESR1,CDKN1A,TP53,NR3C1,MAPK1,AKT1,MAPK8,JUN,RELA,the core components were quercetin,kaempferol,β-sitosterol,stigmasterol,tetrahedron,protoopioid,etc,and the core pathways were PI3K-Akt,MAPK,IL-17,etc.Conclusion:This study preliminarily revealed the mechanism of action of Wuji pill on multi component-multi target-multi pathway of LC,which provided reference for the further clinical development and research of Wuji pill.展开更多
In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether ...In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether the six translations are all quoted.Through detailed analysis and literature review,this paper finds out that the translations of A Dream in Red Mansions are all translated by Liu Wuji,and the Chinese version is the Chengyi Version.展开更多
A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 co...A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 column （250 mm×4.6 mm, 5 μm） as the chromatographic column, and acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution as the mobile phase in a gradient elution with the flow rate of 1.0 mL/min. Sample solution （10 μL） was injected and was tested at the wavelength of 225 nm for 75 min at the column temperature of 30 ℃, Fingerprint similarity software （2004A version） was used to conduct data analysis. A total of 11 batches of Wuji gastric floating sustained-release tablets were tested and analyzed with HPLC fingerprint. Seventeen common peaks were found and the similarity of the 11 batches of agents was greater than 0.9, indicating that the production process of the agent is stable and feasible. The method is operable and could effectively control the quality of Wuji gastric floating sustained-release tablets.展开更多
文摘To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,we predicted the target proteins associated with these active ingredients.In parallel,we employed the GeneCards database to identify genes related to PCOS and determined the intersection of drug targets and disease targets using the online tool available at http://bioinformatics.psb.ugent.be/webtools/Venn/.The shared genes were considered as the target proteins of Wuji powder in the treatment of PCOS.Utilizing the String website,we constructed a protein-protein interaction(PPI)network diagram and identified key protein modules and hub genes within the PPI network using Cytoscape 3.7.1 software.Further analysis in the DAVID database involved GO and KEGG pathway enrichment analyses of the genes within the identified key modules.Our investigation revealed a total of 63 active ingredients in Wuji powder with potential therapeutic effects on PCOS,corresponding to 266 drug targets.Intersection with PCOS-related disease targets yielded 174 shared targets.Ten key modules and ten hub genes(TNF,MMP9,AKT1,ECG,VEGFA,PTGS2,IL-6,MAPK3,STAT3,and CXCL8)were identified through network analysis.KEGG pathway enrichment analysis uncovered 58 signaling pathways,including TNF,MAPK,and PI3K-Akt signaling pathways.GO functional annotation delineated five cellular components(CC),nine molecular functions(MF),and 58 biological processes(BP).Noteworthy findings included extracellular space,enzyme binding,and drug response among CC,MF,and BP categories,respectively.These results collectively suggested that Wuji powder might exert its therapeutic effects on PCOS by modulating the TNF/PI3K/AKT signaling pathway.
基金Supported by Major Social Science Bidding Project of Hunan Province (2010ZDA10)Key Social Science Planning Project of Hengyang City(2010B009)+4 种基金Provincial-level Competitive Course Construction Project of Hunan Universities and Colleges[No33(2006)]Social Science Planning Project of Hengyang City(2009D19)Scientific Foundation of Hengyang Normal University(08C01)Foundation of Key Social Science Research Base of Hengyang CityKey Disciplinary Program of Tourism Management at Hengyang Normal University~~
文摘Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was an important commercial town and a political,economic and cultural center in the lower and middle reaches of Mishui River due to the particular historical opportunities.Its tourist resources are characterized by "integrated natural scenery and ancient town,highly concentrated scenic spots,prosperous business in the history,row upon row of stores and workshops,numerous temples and ancestral halls,and various festivals".It was proposed that the protection and rural tourism development of Wuji Town should be based on consciousness raising,unification of concepts,scientific planning,application of integrated protection means,and development of regional clusters on the basis of famous mountain and city.
基金the General Program of National Natural Science Foundation of China(No.81873399)Major research project of oncology of scientific and technological innovation project of China Academy of Chinese Medical Sciences(No.CI2021A01805).
文摘Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Chinese Pharmacopoeia(2020 Edition)and related literature were searched to identify the main chemical composition and targets of constituent drugs in Wuji pill,including Houpo(Magnoliae Officinalis Cortex),Huangqin(Scutellariae Radix),Badou(Crotonis Fructus),Huanglian(Coptidis Rhizoma)and Renshen(Ginseng Radix et Rhizoma).Meanwhile,Genecards,OMIM,TTD,Drugbank and PharmGkb databases were used to obtain the main targets of LC.The"chemical compound-target"network was constructed by using Cytoscape 3.8.2 software with the disease drug intersection gene as the research target.The PPI network was constructed by using the string database for protein interaction analysis,and the core target genes were sorted out.The Gene ontology go function enrichment analysis and KEGG signal pathway enrichment analysis were carried out based on the ClusterProfiler of R software.Finally,the Autodock Vina software was used to conduct molecular docking between the core target with high value and the key components.Results:A total of 60 active components,190 corresponding targets and 2516 GO and 163 KEGG pathways of Wuji pill were screened out,including 179 potential targets of LC.The core targets were ESR1,CDKN1A,TP53,NR3C1,MAPK1,AKT1,MAPK8,JUN,RELA,the core components were quercetin,kaempferol,β-sitosterol,stigmasterol,tetrahedron,protoopioid,etc,and the core pathways were PI3K-Akt,MAPK,IL-17,etc.Conclusion:This study preliminarily revealed the mechanism of action of Wuji pill on multi component-multi target-multi pathway of LC,which provided reference for the further clinical development and research of Wuji pill.
基金the staged achievement of 2013 China postdoctoral scientific funded program-On the English Translated Version of the Discourse Markers of the Four Classic Works of which Each Chapter Has a Caption(2013M540318)
文摘In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether the six translations are all quoted.Through detailed analysis and literature review,this paper finds out that the translations of A Dream in Red Mansions are all translated by Liu Wuji,and the Chinese version is the Chengyi Version.
基金The National Natural Science Foundation of China(Grant No.81460609)
文摘A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 column （250 mm×4.6 mm, 5 μm） as the chromatographic column, and acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution as the mobile phase in a gradient elution with the flow rate of 1.0 mL/min. Sample solution （10 μL） was injected and was tested at the wavelength of 225 nm for 75 min at the column temperature of 30 ℃, Fingerprint similarity software （2004A version） was used to conduct data analysis. A total of 11 batches of Wuji gastric floating sustained-release tablets were tested and analyzed with HPLC fingerprint. Seventeen common peaks were found and the similarity of the 11 batches of agents was greater than 0.9, indicating that the production process of the agent is stable and feasible. The method is operable and could effectively control the quality of Wuji gastric floating sustained-release tablets.
