Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganiza...Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganization and microvascular endothelial cell hyperpermeability.In this study,we investigated the effects of Xijiao Dihuang Decoction combined with Yinqiao Powder(XDY)on influenza virus(IV)-induced F-actin restructuring and ERM phosphorylation regulated by the Rho/Rho kinase 1(ROCK),p38 MAPK,and PKC signaling pathways in pulmonary microvascular endothelial cells(PMVECs).Methods:Serum containing XDY(XDY-CS;13.8 g/kg)was acquired using standard protocols for serum pharmacology.Primary PMVECs were obtained from male Wistar rats and cultured.After adsorption of IV A(multiplicity of infection,0.01)for 1 h,medium with 20%XDY-CS was added to the PMVECs.The distributions of F-actin and phosphorylated ERM were determined by confocal microscopy,and F-actin expression was measured by flow cytometry.The expression levels of ROCK1,phosphorylated myosin phosphatase target-subunit(p-MYPT),phosphorylated MAPK kinase,phosphorylated p38(p-p38),phosphorylated PKC(p-PKC),and phosphorylated ERM(p-ERM)were determined by western blotting.Results:F-actin reorganization in IV-infected PMVECs was reversed by XDY-CS treatment,which was accompanied by reduced p-ERM production.The p-ERM protein accumulated at plasma membrane of PMVECs infected with IV,which was also inhibited by XDY-CS treatment.展开更多
Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoreti...Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoretic agent Yinqiao power is used to treat patients with fever,not aversion to cold but thirsty"Yinqiao powder is often used to treat Wind-warm syndrome,warm-heat syndrome and miscellaneous diseases caused by some pathogenic factors in Wei-fen syndrome(Wei-fen syndrome is the syndrome name of the disease and is often used to indicate the initial stage of exogenous febrile disease,which is the syndrome of the body's guard surface.)and upper energizer.With the further research and application of Yinqiao powder,it is more and more widely used in clinic.This article summarizes the clinical application of Yinqiao powder through the analysis of the prescription meaning,compatible prescriptions,decoction methods and other aspects of Yinqiao powder in order to provide more evidence for clinical treatment of febrile disease.At the same time,through the prescription thinking of Yinqiao powder,we can learn Wu Jutong's treatment principle and academic thought of"Shangjiao(Shangjiao is one of the three and is the upper part of the Sanjiao.Generally refers to the chest above the diaphragm,including the heart,lungs and throat.)is like a feather,not lightly".Wu Jutong's flexible use of Chinese medicine and the thought of syndrome differentiation and treatment are also worthy of our study and reference.展开更多
目的系统评价银翘散治疗急性上呼吸道感染有效性,为其临床应用提供依据。方法计算机检索中国知网学术期刊(CNKI)、万方数据库服务平台(WanFangData)、维普科技期刊全文数据库(VIP)、中文生物医学文献数据库(CBM)、Pubmed数据库、Web of ...目的系统评价银翘散治疗急性上呼吸道感染有效性,为其临床应用提供依据。方法计算机检索中国知网学术期刊(CNKI)、万方数据库服务平台(WanFangData)、维普科技期刊全文数据库(VIP)、中文生物医学文献数据库(CBM)、Pubmed数据库、Web of Science数据库、The Cochrane Library及Clinical Trials.gov数据库关于银翘散治疗急性上呼吸道感染的随机对照试验,对所纳入研究进行质量筛选、数据提取,并采用RevMan5.3软件进行Meta分析。结果共纳入有效研究17项。Meta分析结果显示:银翘散试验组的临床总有效率优于对照组[相对危险度(RR)=1.19,95%置信区间(CI)(1.15,1.22),P<0.00001];退热时间短于对照组[标准化均数差(SMD)=-0.36,95%CI(-0.71,-0.01),P=0.04];治愈疗程短于对照组[SMD=-2.02,95%CI(-3.24,-0.80),P=0.001];体温恢复正常时间短于对照组[SMD=-2.67,95%CI(-4.06,-1.28),P=0.0002];痊愈率优于对照组[RR=1.54,95%CI(1.35,1.75),P<0.00001];复发率低于对照组[RR=0.06,95%CI(0.01,0.30),P=0.0008];咽喉疼痛消失时间短于[SMD=-3.93,95%CI(-7.70,-0.17),P=0.04];2组差异均具有统计学意义。结论基于目前临床证据,银翘散治疗急性上呼吸道感染具有较好的疗效和安全性;但是由于纳入研究样本较少,质量偏低,可能会影响此结论的真实性和可靠性,因此还需要更多大样本、高质量的随机对照试验来加以证实。展开更多
目的采用网络药理学与分子对接方法探究银翘散“异病同治”小儿急性上呼吸道感染(acute upper respiratory infection in children,AURIC)及小儿病毒性心肌炎(viral myocarditis in children,VMC)的共同分子机制。方法利用TCMSP数据库及...目的采用网络药理学与分子对接方法探究银翘散“异病同治”小儿急性上呼吸道感染(acute upper respiratory infection in children,AURIC)及小儿病毒性心肌炎(viral myocarditis in children,VMC)的共同分子机制。方法利用TCMSP数据库及CNKI数据库筛选银翘散的活性成分及靶点;通过GeneCards数据库和OMIM数据库分别筛选AURIC和VMC的相关靶点,获取银翘散与AURIC、VMC三者的交集靶点;利用Cytoscape 3.9.1软件构建“银翘散—成分—靶点”网络,分析重点活性成分;使用STRING数据库通过构建交集靶点的PPI网络以分析核心靶点;使用DAVID数据库对交集靶点进行GO功能和KEGG通路富集分析;利用Autodock Tools软件对核心靶点及主要成分进行分子对接。结果筛选出银翘散129个活性成分,264个作用靶点;银翘散与AURIC、VMC共119个交集靶点;银翘散—成分—靶点网络的构建得出槲皮素、山柰酚、木犀草素、柚皮素、豆甾醇为排名前5的主要成分;PPI网络构建后得出STAT3、RELA、TNF、MAPK3、AKT1为排名前5的核心靶点;GO功能富集分析获得生物过程533条、细胞组成57条、分子功能94条,其中涉及基因表达的调控、凋亡过程的调控、激素的调控、代谢产物的调控、变老、对外来刺激的反应等生物过程,KEGG通路富集分析获得144条通路。分子对接结果显示,核心靶点与重点活性成分均具有良好的结合活性。结论银翘散主要通过STAT3、RELA、TNF、MAPK3、AKT1等靶点,槲皮素、山柰酚、木犀草素、柚皮素、豆甾醇等主要活性成分和癌症信号通路、PI3K/Akt信号通路、TNF信号通路、AGE/RAGE信号、IL-17信号通路及Toll样受体信号通路等途径对AURIC、VMC发挥“异病同治”作用。展开更多
基金This work was supported by funding from the National Natural Science Foundation of China(Grant Nos.81473520 and 81102697).
文摘Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganization and microvascular endothelial cell hyperpermeability.In this study,we investigated the effects of Xijiao Dihuang Decoction combined with Yinqiao Powder(XDY)on influenza virus(IV)-induced F-actin restructuring and ERM phosphorylation regulated by the Rho/Rho kinase 1(ROCK),p38 MAPK,and PKC signaling pathways in pulmonary microvascular endothelial cells(PMVECs).Methods:Serum containing XDY(XDY-CS;13.8 g/kg)was acquired using standard protocols for serum pharmacology.Primary PMVECs were obtained from male Wistar rats and cultured.After adsorption of IV A(multiplicity of infection,0.01)for 1 h,medium with 20%XDY-CS was added to the PMVECs.The distributions of F-actin and phosphorylated ERM were determined by confocal microscopy,and F-actin expression was measured by flow cytometry.The expression levels of ROCK1,phosphorylated myosin phosphatase target-subunit(p-MYPT),phosphorylated MAPK kinase,phosphorylated p38(p-p38),phosphorylated PKC(p-PKC),and phosphorylated ERM(p-ERM)were determined by western blotting.Results:F-actin reorganization in IV-infected PMVECs was reversed by XDY-CS treatment,which was accompanied by reduced p-ERM production.The p-ERM protein accumulated at plasma membrane of PMVECs infected with IV,which was also inhibited by XDY-CS treatment.
文摘Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoretic agent Yinqiao power is used to treat patients with fever,not aversion to cold but thirsty"Yinqiao powder is often used to treat Wind-warm syndrome,warm-heat syndrome and miscellaneous diseases caused by some pathogenic factors in Wei-fen syndrome(Wei-fen syndrome is the syndrome name of the disease and is often used to indicate the initial stage of exogenous febrile disease,which is the syndrome of the body's guard surface.)and upper energizer.With the further research and application of Yinqiao powder,it is more and more widely used in clinic.This article summarizes the clinical application of Yinqiao powder through the analysis of the prescription meaning,compatible prescriptions,decoction methods and other aspects of Yinqiao powder in order to provide more evidence for clinical treatment of febrile disease.At the same time,through the prescription thinking of Yinqiao powder,we can learn Wu Jutong's treatment principle and academic thought of"Shangjiao(Shangjiao is one of the three and is the upper part of the Sanjiao.Generally refers to the chest above the diaphragm,including the heart,lungs and throat.)is like a feather,not lightly".Wu Jutong's flexible use of Chinese medicine and the thought of syndrome differentiation and treatment are also worthy of our study and reference.
