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兔眼IPE与RPE细胞膜表面K-NPPase酶组织化学的电镜图像分析
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作者 王海瑛 刘哲丽 《国际眼科杂志》 CAS 2006年第2期365-368,共4页
目的:为了探讨自体虹膜色素上皮细胞(IPE)能否代替视网膜色素上皮细胞(RPE)移植到视网膜下腔执行物质转运功能,对兔眼IPE与RPE细胞膜表面的Na+,K+-ATPase采用K-NPPase酶组织化学技术进行定位和分析比较。方法:家兔12只,取IPE和RPE组织固... 目的:为了探讨自体虹膜色素上皮细胞(IPE)能否代替视网膜色素上皮细胞(RPE)移植到视网膜下腔执行物质转运功能,对兔眼IPE与RPE细胞膜表面的Na+,K+-ATPase采用K-NPPase酶组织化学技术进行定位和分析比较。方法:家兔12只,取IPE和RPE组织固定,配制孵育液后进行K-NPPase酶组化孵育,制成电镜标本,采集图像,利用Metamorph/DP10/BX51系统进行分析。随机选取IPE细胞膜以及RPE细胞顶端相邻K-NPPase颗粒两点间的距离进行测量。计算每组样本的均值±标准差(x±s),进行成组设计材料的t检验。结果:有色素组与无色素组汇总后的IPE细胞膜上K-NPPase颗粒的距离平均值40.16±1.19nm,RPE顶端微绒毛细胞膜上K-NPPase颗粒的距离平均值为41.07±1.78nm,两者之间的差异无显著性意义(0.5>P>0.2)。结论:兔眼IPE细胞膜表面与RPE细胞顶端微绒毛膜表面均有Na+,K+-ATPase分布,采用K-NPPas酶组化电镜观察到的兔眼IPE细胞与RPE细胞可能具有相似的物质转运功能。 展开更多
关键词 虹膜色素上皮细胞 视网膜色素上皮细胞 钾依赖性对硝基苯磷酸酶 钠钾ATP酶 物质转运功能
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Evolution of Nitrogen, Phosphorus and Potassium Fertilizer Application Rates in Cotton Fields and Its Influences on Cotton Yield in the Yangtze River Valley
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作者 Naiyin XU Jian LI 《Agricultural Science & Technology》 CAS 2014年第10期1727-1729,1792,共4页
[Objective] The historical evolution pattern of nitrogen (N), phosphorus (P) and potassium (K) fertilizer application rate and its effects on lint cotton yield were explored to provide the theoretical basis for ... [Objective] The historical evolution pattern of nitrogen (N), phosphorus (P) and potassium (K) fertilizer application rate and its effects on lint cotton yield were explored to provide the theoretical basis for reasonable fertilizer management strate-gy in the cotton planting region of the Yangtze River Val ey. [Method] GGE biplot analysis method was adopted to analyze the correlation among N, P and K fertilizer application rate and lint cotton yield with the dataset of national cotton regional trials of the Yangtze River Val ey during 1991-2013. The linear and nonlinear regression analysis method was used to reveal the evolution of the fertilizer applying patterns, and analyze the effects of N, P, K application rates on cotton lint yield. [Result] The application rates of N, P and K fertilizer presented highly significant positive corre-lation with lint cotton yield, among which the potassium fertilizer was the strongest relative factor with lint cotton yield, fol owed by phosphorus fertilizer, while nitrogen fertilizer was the weakest factor. The application rate of nitrogen fertilizer was relat-ed with the test year in the pattern of a quadratic function, while phosphate and potassium had progressive increase linear relation with the test year in the cotton planting region of the Yangtze River Val ey. Meanwhile, cotton lint yield was in re-sponse to nitrogen fertilizer content increase with a quadratic parabola function, and increased with the applying phosphate fertilizer and potassium fertilizer content with linearly increasing function. [Conclusion] The increasing application amount of N, P and K fertilizer was general y beneficial to cotton yield improvements, however, ex-orbitant applying nitrogen fertilizer was unfavorable for cotton production, and a reasonable mixture formula of N, P and K fertilizer was better in terms of cotton yield-increasing effect. 展开更多
关键词 Cotton (Gossypium hirsutum L.) GGE biplot Nitrogen phosphorus andpotassium fertilizer The Yangtze River Valley
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