Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol,so increasing its yield is essential to ensure the sugar security and bioenergy production.Intergeneric hybri...Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol,so increasing its yield is essential to ensure the sugar security and bioenergy production.Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants,particularly those species with high ploidy such as sugarcane(Saccharum spp.).Tripidium arundinaceum exhibits many desirable agronomic traits,and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding.However,the genetic relationship between T.arundinaceum and Saccharum species,and the individual T.arundinaceum chromosomal compositions in sugarcane hybrids are still elusive.Here we used whole-genome single-nucleotide polymorphisms(SNPs)to ascertain the phylogenetic relationships between these species and found that T.arundinaceum is more closely related to Saccharum than Sorghum,in contrast to the previous narrow genetic analyses using chloroplast DNA.Additionally,oligonucleotide(oligo)-based chromosome-specific painting derived from Saccharum officinarum was able to distinctly identify the chromosomes of T.arundinaceum.We developed the oligo-genomic in situ hybridization(GISH)system for the first time,to unveil the novel chromosome translocations and the transmission of individual T.arundinaceum chromosomes in sugarcane progeny.Notably,we discovered that the chromosomal transmission of T.arundinaceum exhibited several different inheritance modes,including n,2n,and over 2n in the BC1 progenies.Such inheritance patterns may have resulted from first division restitution(FDR)or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/second division restitution(FDR+NSC/SDR)model during meiosis.These results will be of substantial benefit for the further selection of T.arundinaceum chromosomes for sugarcane genetic improvement.展开更多
[Objective] This study was conducted to utilize and estimate Erianthus arundinaceum resources and to develop their elite traits. [Method] Phenotypic traits and genetic diversity were evaluated based on 5 quantitative ...[Objective] This study was conducted to utilize and estimate Erianthus arundinaceum resources and to develop their elite traits. [Method] Phenotypic traits and genetic diversity were evaluated based on 5 quantitative traits and 21 qualitative traits of 162 accessions of Erianthus arundinaceum collected from nine provinces in China （74 in Yunnan, 15 in Fujian, 19 in Guizhou, 18 in Hainan, 14 in Sichuan, 10 in Jiangxi, 4 in Guangdong, 4 in Guangxi and 4 in Zhejiang）. [Result] The Shannon-Wiener indices of qualitative traits among the E. arundinaceum populations from different provinces were generally low. The accessions from Fujian exhibited the highest genetic diversity index （0.762 4）, while those from Guangxi showed the lowest one （0.294 2）. There were great genetic variances in quantitative traits, with mean variation coefficients ranging from 14.95% to 32.15%. The accessions from Yunnan showed the highest variation coefficient （32.15%） and those from Guangxi showed the lowest one （14.95%）. Brix exhibited extremely significant nega- tive correlation with altitude and plant height showed extremely significant negative correlation with latitude. Coefficients of genetic divergence indicated that a high proportion of total genetic variation was retained within the populations from different regions, and the high gene flow showed that there were active genetic exchanges among these populations, suggesting no significant genetic divergence among these populations. According to genetic distance and UPGMA, there was a certain correlation between genetic distance and different sampling regions of E. arundinaceum. [Conclusion] The results provide theoretical references for resource collection , het- erosis application and development of excellent genes of sugarcane germplasm.展开更多
基金funded by the Central Government and Local Science and Technology Development Special Project,China(2022L3086)the Sugarcane Research Foundation of Guangxi University,China(2022GZB006)+3 种基金supported by the National Natural Science Foundation of China(31771863)the Academy of Sugarcane and Sugar Industry,Guangxi University,China(ASSI-2023009)an independent fund of Guangxi Key Laboratory of Sugarcane Biology,China(GXKLSCB-20190201)the China Agriculture Research System of MOF and MARA(CARS-20-1-5)。
文摘Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol,so increasing its yield is essential to ensure the sugar security and bioenergy production.Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants,particularly those species with high ploidy such as sugarcane(Saccharum spp.).Tripidium arundinaceum exhibits many desirable agronomic traits,and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding.However,the genetic relationship between T.arundinaceum and Saccharum species,and the individual T.arundinaceum chromosomal compositions in sugarcane hybrids are still elusive.Here we used whole-genome single-nucleotide polymorphisms(SNPs)to ascertain the phylogenetic relationships between these species and found that T.arundinaceum is more closely related to Saccharum than Sorghum,in contrast to the previous narrow genetic analyses using chloroplast DNA.Additionally,oligonucleotide(oligo)-based chromosome-specific painting derived from Saccharum officinarum was able to distinctly identify the chromosomes of T.arundinaceum.We developed the oligo-genomic in situ hybridization(GISH)system for the first time,to unveil the novel chromosome translocations and the transmission of individual T.arundinaceum chromosomes in sugarcane progeny.Notably,we discovered that the chromosomal transmission of T.arundinaceum exhibited several different inheritance modes,including n,2n,and over 2n in the BC1 progenies.Such inheritance patterns may have resulted from first division restitution(FDR)or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/second division restitution(FDR+NSC/SDR)model during meiosis.These results will be of substantial benefit for the further selection of T.arundinaceum chromosomes for sugarcane genetic improvement.
基金Supported by Crop Germplasm Protection Project of Ministry of Agriculture of the People’s Republic of China(2014NWB017)Fund for National Infrastructure of Sugarcane Germplasm Resources(2012-044)Fund for Sugarcane Industry Technical System Construction of Kaiyuan Field Station(CARS-20-6-13)~~
文摘[Objective] This study was conducted to utilize and estimate Erianthus arundinaceum resources and to develop their elite traits. [Method] Phenotypic traits and genetic diversity were evaluated based on 5 quantitative traits and 21 qualitative traits of 162 accessions of Erianthus arundinaceum collected from nine provinces in China （74 in Yunnan, 15 in Fujian, 19 in Guizhou, 18 in Hainan, 14 in Sichuan, 10 in Jiangxi, 4 in Guangdong, 4 in Guangxi and 4 in Zhejiang）. [Result] The Shannon-Wiener indices of qualitative traits among the E. arundinaceum populations from different provinces were generally low. The accessions from Fujian exhibited the highest genetic diversity index （0.762 4）, while those from Guangxi showed the lowest one （0.294 2）. There were great genetic variances in quantitative traits, with mean variation coefficients ranging from 14.95% to 32.15%. The accessions from Yunnan showed the highest variation coefficient （32.15%） and those from Guangxi showed the lowest one （14.95%）. Brix exhibited extremely significant nega- tive correlation with altitude and plant height showed extremely significant negative correlation with latitude. Coefficients of genetic divergence indicated that a high proportion of total genetic variation was retained within the populations from different regions, and the high gene flow showed that there were active genetic exchanges among these populations, suggesting no significant genetic divergence among these populations. According to genetic distance and UPGMA, there was a certain correlation between genetic distance and different sampling regions of E. arundinaceum. [Conclusion] The results provide theoretical references for resource collection , het- erosis application and development of excellent genes of sugarcane germplasm.
