Ilex asprella(Hook.et Arn.)Champ.ex Benth is one of the most important traditional Chinese medicines in southern China.The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteri...Ilex asprella(Hook.et Arn.)Champ.ex Benth is one of the most important traditional Chinese medicines in southern China.The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteristics,which severely impacts the efficiency of seedling raising and increases labor costs.In this study,to improve the seed germination of I.asprella,the effects of germination substrate,hormone,winnowing,and stratification treatments on the seed germination of I.asprella were investigated.The results of the germination matrix showed that the highest germination percentage of 45.2%was achieved under the 20℃/10℃day/night temperature and vermiculite germination medium conditions.The results of hormone treatments revealed that 100–400 mg/L of gibberellin(GA)and 50–100 mg/L of salicylic acid(SA)were found to be effective in releasing the dormancy of I.asprella seeds.Moreover,winnowing could effectively eliminate unsaturated seeds and impurities,thus improving the seed germination of I.asprella.Furthermore,warm temperature(15℃)stratification could expand the temperature range of I.asprella’s seed germination,which was beneficial for seed germination of I.asprella and for seed nursery at room temperature in production practice.The present study obtained a method to break dormancy and increase seed germination in I.asprella,thereby forming a groundwork for improving the efficiency of large-scale planting of I.asprella.展开更多
Phytochemical investigation of the leaves of Ilex aspralla （Hook. et Am.） Champ. ex Benth. led to the isolation of eleven compounds （1-11）. By comparison with those reported data in the literatures their structure...Phytochemical investigation of the leaves of Ilex aspralla （Hook. et Am.） Champ. ex Benth. led to the isolation of eleven compounds （1-11）. By comparison with those reported data in the literatures their structures were identified as kaempferol-3-O-β-D- 6＂-acetylglucopyranoside （1）, kaempferol-4＇-methylether （2）, quercetin （3）, quercetin-3-O-glucopyranoside （4）, tormentoside （5）, suavissimoside R1 （6）, 3β,19α-dihydroxyolean-12-ene-24,28-dioicacid-28-O-β-D-glucopyranoside （7）, 3-O-β-sulfooxy-19-hydroxy- urs-12-ene-28-oic acid （8）, coniferin （9）, adenoside （10）, 1＇-O-benzyl-ct-L-rhamnopyranosyl-（l＂→6＇）-β-D-glucopyranoside （11）. Among these, six compounds （1, 2, 5, 9-11） were firstly reported from the genus Ilex, and five compounds （3, 4, 6-8） were isolated for the first time from llex aspralla.展开更多
Background The gradually increasing changes in a human hyperlipidemic diet along with chronic stress might play an important role in the increased numbers of fatty liver. This study investigated the effects of Ilex as...Background The gradually increasing changes in a human hyperlipidemic diet along with chronic stress might play an important role in the increased numbers of fatty liver. This study investigated the effects of Ilex asprella root decoction on related genes of lipid metabolism in chronic stress in hyperlipidemic fatty liver in rats. Methods Forty-eight male Wistar rats were randomly divided into four groups： normal control group, model control group, simvastatin group, and Ilex asprella root group. To establish chronic stress and hyperlipidemic fatty liver models in rats, the levels of serum lipids, glucose, liver index, insulin （INS）, insulin resistant （IR） index, adiponectin, superoxide dismutase （SOD）, glutathione peroxidase （GSH-pX）, glutathione （GSH）, liver X receptor （LXR）, and sterol responsive element binding protein （$REBP）-lc in rats were measured. Results When compared to the normal control group, the levels of serum lipids, glucose, liver index, INS, IR index, and GSH in the model control group significantly increased （P 〈0.01）. The protein levels of LXRa and SREBP-lc increased （P 〈0.05）, and the serum adiponectin and the SOD and GSH-pX decreased significantly （P 〈0.01）. When compared to the model control group, the levels of serum lipids, glucose, liver index, INS, IR index, SOD, and GSH-pX in the simvastatin group and Ilex asprella root group increased in varying degrees （P 〈0.01 or 0.05）; the serum adiponectin and GSH decreased （P 〈0.05）, while the protein levels of LXRa and SREBP-lc decreased in varying degrees （P 〈0.01 or 0.05）. When compared to the simvastatin group, the IR index and protein levels of LXRa in the Ilex asprella root group decreased （P 〈0.05）, and the serum adiponectin and SOD increased （P 〈0.05）. Conclusion The Ilex asprella root decoction has some protective effects on regulating the related genes of lipid metabolism caused by chronic stress and hyperlipidemic fatty liver in rats.展开更多
基金supported by the Fund Projects of the Central Government in Guidance of Local Science and Technology Development(GuiKeZY22096020)Natural Science Foundation of Guangxi(2019GXNSFBA245073)+1 种基金National Natural Science Foundation of China(82260750,82260749)Cooperative Project of Guangxi Botanical Garden of Medicinal Plants with China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.(202112-1).
文摘Ilex asprella(Hook.et Arn.)Champ.ex Benth is one of the most important traditional Chinese medicines in southern China.The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteristics,which severely impacts the efficiency of seedling raising and increases labor costs.In this study,to improve the seed germination of I.asprella,the effects of germination substrate,hormone,winnowing,and stratification treatments on the seed germination of I.asprella were investigated.The results of the germination matrix showed that the highest germination percentage of 45.2%was achieved under the 20℃/10℃day/night temperature and vermiculite germination medium conditions.The results of hormone treatments revealed that 100–400 mg/L of gibberellin(GA)and 50–100 mg/L of salicylic acid(SA)were found to be effective in releasing the dormancy of I.asprella seeds.Moreover,winnowing could effectively eliminate unsaturated seeds and impurities,thus improving the seed germination of I.asprella.Furthermore,warm temperature(15℃)stratification could expand the temperature range of I.asprella’s seed germination,which was beneficial for seed germination of I.asprella and for seed nursery at room temperature in production practice.The present study obtained a method to break dormancy and increase seed germination in I.asprella,thereby forming a groundwork for improving the efficiency of large-scale planting of I.asprella.
基金National Key Technology R&D Program "New Drug Innovation" of China (Grant No.2012ZX09201-201-001,2012ZX09301002-002-002 and 2012ZX09304-005)Doctoral Fund of Ministry of Education (Grant No.20110001130003)
文摘Phytochemical investigation of the leaves of Ilex aspralla （Hook. et Am.） Champ. ex Benth. led to the isolation of eleven compounds （1-11）. By comparison with those reported data in the literatures their structures were identified as kaempferol-3-O-β-D- 6＂-acetylglucopyranoside （1）, kaempferol-4＇-methylether （2）, quercetin （3）, quercetin-3-O-glucopyranoside （4）, tormentoside （5）, suavissimoside R1 （6）, 3β,19α-dihydroxyolean-12-ene-24,28-dioicacid-28-O-β-D-glucopyranoside （7）, 3-O-β-sulfooxy-19-hydroxy- urs-12-ene-28-oic acid （8）, coniferin （9）, adenoside （10）, 1＇-O-benzyl-ct-L-rhamnopyranosyl-（l＂→6＇）-β-D-glucopyranoside （11）. Among these, six compounds （1, 2, 5, 9-11） were firstly reported from the genus Ilex, and five compounds （3, 4, 6-8） were isolated for the first time from llex aspralla.
文摘Background The gradually increasing changes in a human hyperlipidemic diet along with chronic stress might play an important role in the increased numbers of fatty liver. This study investigated the effects of Ilex asprella root decoction on related genes of lipid metabolism in chronic stress in hyperlipidemic fatty liver in rats. Methods Forty-eight male Wistar rats were randomly divided into four groups： normal control group, model control group, simvastatin group, and Ilex asprella root group. To establish chronic stress and hyperlipidemic fatty liver models in rats, the levels of serum lipids, glucose, liver index, insulin （INS）, insulin resistant （IR） index, adiponectin, superoxide dismutase （SOD）, glutathione peroxidase （GSH-pX）, glutathione （GSH）, liver X receptor （LXR）, and sterol responsive element binding protein （$REBP）-lc in rats were measured. Results When compared to the normal control group, the levels of serum lipids, glucose, liver index, INS, IR index, and GSH in the model control group significantly increased （P 〈0.01）. The protein levels of LXRa and SREBP-lc increased （P 〈0.05）, and the serum adiponectin and the SOD and GSH-pX decreased significantly （P 〈0.01）. When compared to the model control group, the levels of serum lipids, glucose, liver index, INS, IR index, SOD, and GSH-pX in the simvastatin group and Ilex asprella root group increased in varying degrees （P 〈0.01 or 0.05）; the serum adiponectin and GSH decreased （P 〈0.05）, while the protein levels of LXRa and SREBP-lc decreased in varying degrees （P 〈0.01 or 0.05）. When compared to the simvastatin group, the IR index and protein levels of LXRa in the Ilex asprella root group decreased （P 〈0.05）, and the serum adiponectin and SOD increased （P 〈0.05）. Conclusion The Ilex asprella root decoction has some protective effects on regulating the related genes of lipid metabolism caused by chronic stress and hyperlipidemic fatty liver in rats.
