Fragmented data suggest that bisphenol AF(BPAF),a chemical widely used in a variety of products,might have potential impacts on the hypothalamus.Here,we employed male neonatal mice following maternal exposure to explo...Fragmented data suggest that bisphenol AF(BPAF),a chemical widely used in a variety of products,might have potential impacts on the hypothalamus.Here,we employed male neonatal mice following maternal exposure to explore the effects of low-dose BPAF on hypothalamic development by RNA-sequencing.We found that maternal exposure to approximately 50μg/(kg·day)BPAF from postanal day(PND)0 to PND 15 altered the hypothalamic transcriptome,primarily involving the pathways and genes associated with extracellular matrix(ECM)and intercellular adhesion,neuroendocrine regulation,and neurological processes.Further RNA analysis confirmed the changes in the expression levels of concerned genes.Importantly,we further revealed that low-dose BPAF posed a stimulatory impact on pro-opiomelanocortin(POMC)neurons in the arcuate nucleus of the hypothalamus and induced the browning of inguinal white adipose tissue.All findings indicate that developmental exposure to low-dose BPAF could interfere with hypothalamic development and thereby lead to alterations in the metabolism.Interestingly,5000μg/(kg·day)BPAF caused slighter,non-significant or even inverse alterations than the low dose of 50μg/(kg·day),displaying a dose-independent effect.Further observations suggest that the the dose-independent effects of BPAF might be associated with oxidative stress and inflammatory responses caused by the high dose.Overall,our study highlights a risk of low-dose BPAF to human neuroendocrine regulation and metabolism.展开更多
Bisphenol(BP)analogues in wastewater effluent and groundwater pose a potential threat to human health due to their ability to disrupt steroidogenesis.A new solar-assisted electrochemical process(SECP)was developed and...Bisphenol(BP)analogues in wastewater effluent and groundwater pose a potential threat to human health due to their ability to disrupt steroidogenesis.A new solar-assisted electrochemical process(SECP)was developed and evaluated for the degradation of BP analogues.The effects of quenchers,current density,initial pH,supporting electrolyte,and aqueous matrix on the removal kinetics of bisphenol AF(BPAF)and bisphenol A(BPA)were investigated.The kinetic constants of BPAF,BPA,and bisphenol S(BPS)in the SECP with irradiation intensity of 500 mW cm^(-2) were 0.017±0.002 min^(-1),0.022±0.002 min^(-1),and 0.012±0.001 min^(-1),respectively.The changes in the degradation rates of BPAF,BPA,and BPS in the presence of quenchers indicated the relative contribution of hydroxyl radical(·OH)oxidation,anodic electrolysis,and singlet(^(1)O_(2))oxygenation in the degradation of BPs in the SECP.The enhanced rate of generation of ·OH and ^(1)O_(2) was observed in the SECP compared with those in the conventional electrochemical system.The identification of the transformation products(TPs)of BPAF demonstrated that hydroxylation,ring cleavage,b-scission,and defluorination were the major processes during the oxidation in the SECP.The conversion to fluoride ions(76%)and mineralization of total organic carbon(72%)in the SECP indicated further degradation of TPs.The results from this study improved our understanding of the degradation of BP analogues in the electrooxidation irradiated by solar light and help to establish the application potential of the SECP for the effective degradation of emerging contaminants in wastewater.展开更多
Bisphenol AF(BPAF),an alternative to bisphenol A,is widely detected in aquatic environments.Owing to health concerns,the toxic effects of BPAF on organisms are drawing attention.The present study aims to evaluate the ...Bisphenol AF(BPAF),an alternative to bisphenol A,is widely detected in aquatic environments.Owing to health concerns,the toxic effects of BPAF on organisms are drawing attention.The present study aims to evaluate the toxicity of BPAF,combining the results of omics techniques and experiment.Employing transcriptome sequencing(RNA-seq),we obtained 391,648,512,and 545 differentially expressed genes(DEGs)in 0.1,1,10,and 100 mg/L BPAF-exposed zebrafish larvae,respectively.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment revealed the early development,stimulus-response,and MAPK signaling pathway were significantly affected by BPAF.In addition,five hub genes(fgf3,fgf4,map2k1,myca,and casp3b)were highlighted as the key genes in MAPK signaling pathway using the protein-protein interaction network.Therefore,the RNA-seq results showed that early development and stimulus-response were the main processes affected by BPAF,which was consistent with our morphological and pathological results.The hatching rate of zebrafish embryos in 1 and 10 mg/L BPAF groups was significantly inhibited,and the oxidative stress indexes,including the level of total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and lipid peroxidation(LPO),were significantly increased by the 100 mg/L BPAF treatment.Moreover,the activity of alkaline phosphatase(AKP)was significantly decreased in all BPAF exposure groups.In conclusion,exposure to BPAF at environmental relevant concentrations affected the early development and immune system of zebrafish larvae by modulating MAPK signaling pathway,and our results provide solid evidence for the future studies on the toxicity of bisphenols.展开更多
碱性条件下，双酚 AF 紫外光谱的吸收峰会发生红移，从225 nm 移至248 nm，据此建立了简单、灵敏、快捷、选择性较好的测定双酚 AF 可见分光光度法，并研究了氨水用量、反应时间、反应温度对吸光度的影响。结果表明：双酚 AF 的最大吸...碱性条件下，双酚 AF 紫外光谱的吸收峰会发生红移，从225 nm 移至248 nm，据此建立了简单、灵敏、快捷、选择性较好的测定双酚 AF 可见分光光度法，并研究了氨水用量、反应时间、反应温度对吸光度的影响。结果表明：双酚 AF 的最大吸收波长为248 nm，线性范围是0.6～7．2 mg／L，相关系数 r 为0.9998，为检测食品塑料包装中双酚 AF 的残留提供了新的实验方法。展开更多
基金supported by the National Natural Science Foundation of China(No.21677166)the National Key Research and Development Program of China(No.2018YFA0901103)。
文摘Fragmented data suggest that bisphenol AF(BPAF),a chemical widely used in a variety of products,might have potential impacts on the hypothalamus.Here,we employed male neonatal mice following maternal exposure to explore the effects of low-dose BPAF on hypothalamic development by RNA-sequencing.We found that maternal exposure to approximately 50μg/(kg·day)BPAF from postanal day(PND)0 to PND 15 altered the hypothalamic transcriptome,primarily involving the pathways and genes associated with extracellular matrix(ECM)and intercellular adhesion,neuroendocrine regulation,and neurological processes.Further RNA analysis confirmed the changes in the expression levels of concerned genes.Importantly,we further revealed that low-dose BPAF posed a stimulatory impact on pro-opiomelanocortin(POMC)neurons in the arcuate nucleus of the hypothalamus and induced the browning of inguinal white adipose tissue.All findings indicate that developmental exposure to low-dose BPAF could interfere with hypothalamic development and thereby lead to alterations in the metabolism.Interestingly,5000μg/(kg·day)BPAF caused slighter,non-significant or even inverse alterations than the low dose of 50μg/(kg·day),displaying a dose-independent effect.Further observations suggest that the the dose-independent effects of BPAF might be associated with oxidative stress and inflammatory responses caused by the high dose.Overall,our study highlights a risk of low-dose BPAF to human neuroendocrine regulation and metabolism.
基金the support from the State Key Laboratory of Urban Water Resource and Environment(QA201926)Youth program of the National Natural Science Foundation of China(51908164)+1 种基金support from the University of Cincinnati through a UNESCO co-Chair Professor position on“Water Access and Sustainability”the Herman Schneider Professorship in the College of Engineering and Applied Sciences.
文摘Bisphenol(BP)analogues in wastewater effluent and groundwater pose a potential threat to human health due to their ability to disrupt steroidogenesis.A new solar-assisted electrochemical process(SECP)was developed and evaluated for the degradation of BP analogues.The effects of quenchers,current density,initial pH,supporting electrolyte,and aqueous matrix on the removal kinetics of bisphenol AF(BPAF)and bisphenol A(BPA)were investigated.The kinetic constants of BPAF,BPA,and bisphenol S(BPS)in the SECP with irradiation intensity of 500 mW cm^(-2) were 0.017±0.002 min^(-1),0.022±0.002 min^(-1),and 0.012±0.001 min^(-1),respectively.The changes in the degradation rates of BPAF,BPA,and BPS in the presence of quenchers indicated the relative contribution of hydroxyl radical(·OH)oxidation,anodic electrolysis,and singlet(^(1)O_(2))oxygenation in the degradation of BPs in the SECP.The enhanced rate of generation of ·OH and ^(1)O_(2) was observed in the SECP compared with those in the conventional electrochemical system.The identification of the transformation products(TPs)of BPAF demonstrated that hydroxylation,ring cleavage,b-scission,and defluorination were the major processes during the oxidation in the SECP.The conversion to fluoride ions(76%)and mineralization of total organic carbon(72%)in the SECP indicated further degradation of TPs.The results from this study improved our understanding of the degradation of BP analogues in the electrooxidation irradiated by solar light and help to establish the application potential of the SECP for the effective degradation of emerging contaminants in wastewater.
基金sponsored by National Natural Science Foundation of China(Grants 21707064)National Key R&D Program of China(2018YFC0406504)+2 种基金Shenzhen Science and Technology Innovation Committee(Grant Number KQJSCX20180322152024270,JCYJ20180302180205159,KQTD2016022619584022)State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control(No.2017B030301012)the Leading Talents of Guangdong Province Program(Chunmiao Zheng).
文摘Bisphenol AF(BPAF),an alternative to bisphenol A,is widely detected in aquatic environments.Owing to health concerns,the toxic effects of BPAF on organisms are drawing attention.The present study aims to evaluate the toxicity of BPAF,combining the results of omics techniques and experiment.Employing transcriptome sequencing(RNA-seq),we obtained 391,648,512,and 545 differentially expressed genes(DEGs)in 0.1,1,10,and 100 mg/L BPAF-exposed zebrafish larvae,respectively.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment revealed the early development,stimulus-response,and MAPK signaling pathway were significantly affected by BPAF.In addition,five hub genes(fgf3,fgf4,map2k1,myca,and casp3b)were highlighted as the key genes in MAPK signaling pathway using the protein-protein interaction network.Therefore,the RNA-seq results showed that early development and stimulus-response were the main processes affected by BPAF,which was consistent with our morphological and pathological results.The hatching rate of zebrafish embryos in 1 and 10 mg/L BPAF groups was significantly inhibited,and the oxidative stress indexes,including the level of total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and lipid peroxidation(LPO),were significantly increased by the 100 mg/L BPAF treatment.Moreover,the activity of alkaline phosphatase(AKP)was significantly decreased in all BPAF exposure groups.In conclusion,exposure to BPAF at environmental relevant concentrations affected the early development and immune system of zebrafish larvae by modulating MAPK signaling pathway,and our results provide solid evidence for the future studies on the toxicity of bisphenols.
文摘碱性条件下，双酚 AF 紫外光谱的吸收峰会发生红移，从225 nm 移至248 nm，据此建立了简单、灵敏、快捷、选择性较好的测定双酚 AF 可见分光光度法，并研究了氨水用量、反应时间、反应温度对吸光度的影响。结果表明：双酚 AF 的最大吸收波长为248 nm，线性范围是0.6～7．2 mg／L，相关系数 r 为0.9998，为检测食品塑料包装中双酚 AF 的残留提供了新的实验方法。
