Effects of Radix Puerariae, Radix Rehmanniae and Their Compatibility on Blood Glucose and Blood Lipids in Mice

[Objectives]To explore the effects of the compatibility of Radix Puerariae and Radix Rehmanniae on blood glucose and blood lipids in diabetic mouses.[Methods]Diabetic mouse model was established.The body weight and fasting blood glucose of mice were measured after 7 and 14 d of administration,and the biochemical indicators of blood lipids(TC,HDL-C,and LDL-C)were detected after 14 d of administration.[Results]Compared with the Radix Puerariae group and Radix Rehmanniae group,the compatibility group(1:2)had the best hypoglycemic effect(P<0.05),and TC and LDL-C in the compatibility group(2:1)significantly decreased(P<0.05),while HDL-C in the compatibility group(1:1)significantly increased(P<0.05).[Conclusions]Radix Puerariae,Radix Rehmanniae and their combination can reduce the blood glucose of diabetic mice.The compatibility group(1:2)had a significant hypoglycemic effect(P<0.05),and LDL-C in the compatibility group(2:1)significantly declined,while HDL-C in the compatibility group(1:1)rose significantly.

Analysis of Blood Lipids, Blood Glucose, Blood Uric Acid, and Blood Routine Test Results in Retired Employees of a Unit in the Civil Aviation System

Objective:To investigate and analyze the annual physical examination results of retired employees from a unit in the civil aviation system,focusing on blood lipids,blood glucose,blood uric acid,and blood routine results.The study aims to provide relevant references for formulating reasonable disease management measures for preventing and controlling hyperlipidemia,hyperuricemia,and other conditions in retired employees.Methods:The examination results of 231 participants were collected and analyzed.The participants were divided into four groups based on age:middle-aged group,young-old group,middle-old group,and old-old group.The blood test results were compared across these groups,and an assessment of atherosclerotic cardiovascular disease(ASCVD)risk levels was completed in conjunction with medical history.Blood test results were also compared by gender.Results:There were no significant statistical differences in blood test results when grouped by age.However,the prevalence of hyperuricemia was higher in males than in females,while the prevalence of hypercholesterolemia was higher in females than in males.The LDL-C target achievement rate was lower in the moderate-and-high-risk group as well as the very high-risk group as defined by ASCVD risk levels.Conclusion:Management of hyperuricemia and hyperlipidemia in retired employees(elderly patients)should be strengthened to reduce the risk of ASCVD events and alleviate the potential medical burden associated with disease progression.

Lowering of Blood Lipid Levels with a Combination of Pitavastatin and Ezetimibe in Patients with Coronary Heart Disease:A Meta-Analysis

Objectives:According to the findings of randomized controlled trials,blood lipid levels in patients with coronary heart disease(CHD)can be significantly decreased through a combination of pitavastatin and ezetimibe;however,the effects and clinical applications of this treatment remain controversial.This meta-analysis was aimed at objectively assessing the efficacy and safety of pitavastatin and ezetimibe in lowering blood lipid levels.Design:Relevant studies were retrieved from electronic databases,including PubMed,Cochrane Library,Embase,China National Knowledge Infrastructure,VIP,and WanFang Data,from database inception to June 8,2022.The lev-els of low-density lipoprotein cholesterol,total cholesterol,triglycerides,and high-density lipoprotein cholesterol in patients’serum after treatment were the primary endpoint.Results:Nine randomized controlled trials(2586 patients)met the inclusion criteria.The meta-analysis indi-cated that pitavastatin plus ezetimibe resulted in significantly lower levels of LDL-C[standardized mean difference(SMD)=−0.86,95%confidence interval(CI)(−1.15 to−0.58),P<0.01],TC[SMD=−0.84,95%CI(−1.10 to−0.59),P<0.01],and TG[SMD=−0.59,95%CI(−0.89 to−0.28),P<0.01]than pitavastatin alone.Conclusions:Pitavastatin plus ezetimibe significantly decreased serum LDL-C,TC,and TG levels in patients with CHD.

The effects of smoking on blood lipids, C-reactive protein, and homocysteine in young patients with ischemic stroke

Objective:To investigate clinical significance of the effects of smoking on blood lipids, C-reactive protein,and homocysteine in young ischemic stroke patients.Methods:The clinical data of 423 young stroke patients in the department of neurology at Taihe Hospital in Shiyan City, China were retrospectively analyzed, including age,gender,drinking history,family history,and atrial fibrillation history. Patients were divided into two groups according to whether they smoked,and the blood lipids, C-reactive protein, and homocysteine were compared between groups.Results:The proportion of smokers was 41.83%.The levels of total cholesterol,triglycerides (TG), low density lipoprotein (LDL), and homocysteine were higher in patients who smoked than in those who did not(P < 0.05). High density lipoprotein (HDL) was lower in the smoking group (P < 0.05). C-reactive protein test results were divided into groups according to whether the levels exceeded the normal range or not, and no correlation was found between C-reactive protein levels and smoking(P>0.05). Conclusion:Total cholesterol, TG, LDL, HDL, and homocysteine were significantly different between stroke patients who smoked and those who did not. We therefore suggest that smoking cessation take place as soon as possible and that it be avoided entirely in order to reduce the incidence of atherosclerosis and stroke.

Effects of moxibustion at 46°C on blood lipids and related indicators of thoracic aortic endothelium in a hyperlipidemia rat model

Objective:To observe the effects of moxibustion at different temperatures(38℃,46℃)on blood lipids,endothelial morphology of the thoracic aorta,serum endothelin-1(ET-1),calcitonin gene-related peptide(CGRP),nitric oxide(NO),and endothelial NO synthase(eNOS)in hyperlipidemic rats.Methods:Using the random number table method,60 Sprague Dawley rats were randomly and evenly divided into blank,model,38℃-moxibustion,and 46℃-moxibustion groups.Rats in the 3 experimental groups were fed a high-fat feed to model hyperlipidemia in rats.Rats in the 38℃-moxibustion and 46℃-moxibustion groups were moxibustion on the Shenque and bilateral Zusanli acupoints for 10 minutes each,once every other day for 4 weeks,at temperatures of 38±1℃ and 46±1℃.After that,rat blood samples were collected to detect blood lipids and ET-1,CGRP,eNOS and NO.Take the endotheal tissue of the thoracic aorta to do HE staining.Results:(1)The serum total cholesterol,triglycerides,and low-density lipoprotein cholesterol of rats in the 46℃-moxibustion group were significantly lower than those in the model and 38℃-moxibustion groups.(2)Revealed by hematoxylin and eosin staining,showed necrosis in the local vascular endothelial cells and mild inflammatory cell infiltration in the tunica adventitia of the hyperlipidemic rats.These endothelial morphologies did not improve significantly after moxibustion at 38℃ but did improve at 46℃.(3)Compared with the blank group,serum ET-1 was significantly higher and serum CGRP,NO,eNOS were significantly lower in the model group.Compared with the model and the 38℃-moxibustion groups,serum ET-1 was significantly lower and serum CGRP,NO,eNOS were significantly higher in 46℃-moxibustion groups.Conclusion:Moxibustion at 46℃ effectively regulated blood lipids,improved the morphology of the vascular endothelium,and protected vascular endothelial function.

Effects of Perilla Seed on Blood Lipid Regulation and Lipid Peroxidation of Coturnix coturnix with Hyperlipidemia

[ Objective ] Study on the effect of perilla seed on blood lipid regulation and peroxidation of Coturnix coturnix with hyperlipidemia. [ Method ] The high-lipid.diet was fed to establish the high lipid model of Coturnix coturnix. The normal control group, high lipid model group, high dose of perilla seed group（2.5 g/kg）, low dose of perilla seed group （0.83 g/kg） were designed to measure the contents of total cholesterol （TC）, three acids glyceride （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, very low density lipoprotein cholesterol （VLDL-C） and malondialdehyde （MDA）, superoxide dismutase （SOD） activity. [ Result] All the test groups could decrease TC ,TG,LDL-C and MDA content and significantly increase the HDL-C content （p 〈 0.01） and SOD activity（p 〈 0.01 ） in serum. [ Conclusion] Perilla seed has the blood lipid regulation and peroxidation function.

Combined MELD and blood lipid level in evaluating the prognosis of decompensated cirrhosis

AIM: To evaluate the prognostic value of the combined model for end-stage liver disease (MELD) and blood lipid level in patients with decompensated cirrhosis. METHODS: A total of 198 patients with decompensated cirrhosis were enrolled into the study. The values of triglyceride (TG), cholesterol (TC), high density lipoproteins (HDL) and low density lipoprotein (LDL) of each patient on the fi rst day of admission were retrieved from the medical records, and MELD was calculated. All the patients were followed up for 1 year. The relationship between the change of blood lipid level and the value of MELD score was studied by analysis of variance. The prognostic factors were screened by multivariate Cox proportional hazard model. Draw Kaplan-Meier survival curves were drawn. RESULTS: Forty-f ive patients died within 3 mo and 83 patients died within 1 year. The levels of TG, TC, HDL and LDL of the death group were all lower than those of the survivors. The serum TG, TC, HDL and LDL levels were lowered with the increase of the MELD score. Multivariate Cox proportional hazard model showed that MELD ≥18 and TC ≤2.8 mmol/L were independent risk factors for prognosis of decompensated cirrhosis. Survival analysis showed that MELD ≥18 combined with TC ≤ 2.8 mmol/L can clearly discriminate between the patients who would survive and die in 1 year. CONCLUSION: MELD ≥18 and TC ≤2.8 mmol/L are two important indexes to predict the prognosis of patients with decompensated cirrhosis. Their combination can effectively predict the long-term prognosis of patients with decompensated cirrhosis.

Effects of tanshinone Ⅱ sodium sulfonate plus cinepazide maleate on the hemorrheologic indexes and blood lipids in patients with acute cerebral infarction

BACKGROUND： The severity of cerebral infarction is associated with the increase of blood viscosity caused by hyperfibrinogenemia and hyperlipidemia, etc. Thus it has become one of the target for treating cerebral infarction to decrease blood viscosity by integrated Chinese and western medicine. OBJECTIVE： To investigate the influence and clinical therapeutic effects of cinepazide maleate combined with tanshinone Ⅱ A sodium sulfonate on the hemorrheologic indexes and blood lipids of patients with acute cerebral infarction, and compare the results with those of simple cinepazide maleate treatment. DESIGN： A non-randomized case-controlled observation. SETTINGS： Hebei North University; the Second Affiliated Hospitals of Hebei North University; the Third Affiliated Hospitals of Hebei North University, PARTICIPANTS： Eighty-six inpatients with cerebral infarction were selected from the infirmary, the Second and Third Affiliated Hospitals of Hebei North University from September 2004 to October 2006. They were all diagnosed to have acute cerebral infarction by CT or MRI, and accorded with the diagnostic standards for acute cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1995. Meanwhile, 40 teachers and medical staff of voluntary physical examinees were selected as the control group. Informed contents were obtained from all the patients and their relatives. METHODS： The patients were divided into combined treatment group （n=43） and simple treatment group （n=3）. In the combined treatment group, the patients were administrated with 160 mg cinepazide maleate injection （Beijing Four-ring Pharmaceutical, Co.,Ltd, No. H200220125; 80 mg/2 mL） added in 5% glucose, and 40 mg tanshinone Ⅱ sodium sulfonate （Shanghai No.1 Biochemical ＆ Pharmaceutical Co.,Ltd., No. H31022558, 10 mg/2 mL） added in 250 mL normal saline. In the simple treatment group, the patients were only administrated with cinepazide maleate 320 mg added in 5% glucose or 250 mL normal saline. They were treated for 1 or 2 courses, once a day, and 14 days as a course. The patients were detected before treatment and at 14 and 28 days after treatment respectively. ① Determination of hemorrheologic indexes： Whole blood viscosity was determined with LBY-N6B automatic hemorrheologic meter; Plasma viscosity with LBY-F200B automatic plasma viscosity meter; Volume of fibrinogen was determined by the method of 12.5% sodium nitrate depositing biuret reaction. ② Determination of blood lipids： The serum levels of total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） were determined. ③ Severity of neurological deficit： The total score of neurological deficit score （NDS） ranged from 0 to 45 points, 0 - 15 points was taken as mild, 16 - 30 points as moderate and 31 - 45 points as severe.④ Evaluation of curative effects： Generally cured： NDS decreased by 91% - 100%, and disabled severity of grade 0; Significantly improved： NDS decreased by 46% - 90%, and disabled severity of grades 1 - 3; Improved： NDS decreased by 18% - 45%; No change： NDS decreased by less than 18%; Aggravated： NDS increased by more than 18%. Generally cured and significant improved were taken as significant effect. ⑤ The adverse events and side effects after medication were observed. MAIN OUTCOME MEASURES： ① Results of hemorrheologic indexes and blood lipids; ② NDS results in the combined treatment group and simple treatment group; ③ Therapeutic effects and adverse events. RESULTS： All the 86 patients with cerebral infarction and 40 healthy controls were involved in the analysis of results. ① Results of hemorrheologic indexes and blood lipids： The hemorrheologic indexes and blood lipids before treatment were manifested as abnormalities to different extents in both the combined treatment group and simple treatment group; The hemorrheologic indexes after treatment were obviously improved in both groups. But the hemorrheologic indexes were improved more obviously in the combined treatment group as compared with those in the simple treatment group （P 〈 0.05）; The levels of TC, TG and LDL-C after treatment in the combined treatment group were obviously lowered （P 〈 0.05）, whereas those in the simple treatment group were not significantly changed （P 〉 0.05）. ② NDS results： The NDS scores at 14 and 28 days after treatment in the combined treatment group [（6.23±2.34）, （4.27± 1.83） points] were obviously lower than those in the simple treatment group [（8.76±3.41）, （6.65±2.49） points, P 〈 0.05]. ③ Therapeutic effects and side effects： The total significant effective rates in the combined treatment group and simple treatment group were 93% and 81% respectively. In the combined treatment group, 1 case suffered from palpitation, dizziness and agrypnia. In the simple treatment group, 1 case suffered from palpitation, dizziness and agrypnia, 1 case had itch of skin. All the above symptoms disappeared gradually after the transfusing speed was adjusted to be slower. No drug withdrawal occurred in the patients due to the adverse events. CONCLUSION： Cinepazide maleate combined with tanshinon can obviously improve the abnormalities of hemorrheologic indexes and blood lipids and nerve function in patients with acute cerebral infarction, and its curative effect is faster than that of simple cinepazide maleate treatment.

Effects of SNPs at Newly Identified Lipids Loci on Blood Lipid Levels and Risk of Coronary Heart Disease in Chinese Han Population:A Case Control Study

Associations between ＂lipid-related＂ candidate genes,blood lipid concentrations and coronary artery disease（CHD） risk are not clear.We aimed to investigate the effect of three newly identified lipids loci from genome-wide association studies on CHD and blood lipid levels in Chinese Han population.The genotypes of SNPs at three newly identified lipid loci and blood lipids concentrations were examined in 1360 CHD patients and 1360 age-and sex-frequency matched controls from an unrelated Chinese Han population.Allele T of rs16996148 occurred less frequently in CHD patients with the odds ratio（OR） being 0.64（95% CI 0.50 to 0.81）,after adjusting for conventional risk factors and was associated with a 33% decreased CHD risk（P0.01） comparing with the major allele G.Individuals with GT genotype had the lowest CHD risk.No associations were found between the polymorphisms of other two loci with CHD risk and all three SNPs had no effect on lipid profile in this population.SNP rs16996148 on chromosome 19p13 is significantly associated with lower risk for CHD in Chinese Han population.However,it remains unresolved why these lipid-related loci had significantly less effects than the correspondingly expected effects on blood lipids levels in this population.

Study on the Effects of Reducing Blood Lipid by Flavonoids from Ampelopsis grossedentata on Hyperlipidemia Rats

In order to study the effects of Ampelopsis grossedentata on endogenous cholesterol biosynthesis and the effects of reducing blood lipid in hyperlipidemia rats,extraction of flavonoids from Ampelopsis grossedentata by using ethanol(TFAG),and the effects of TFAG on intracellular cholesterol synthesis were detected by amphotericin B-cell model;SD hyperlipidemia rat model was established by feeding high fat diet.A formulated medicine called Xuezhikang was used as a positive control,and TFAG of different doses were administered to the stomach for 30 d continuously to measure the indexes of heart,liver tissue homogenate and serum;part of the liver was taken for pathological observation.The results showed that TFAG could significantly inhibit the synthesis of cholesterol in cells.TFAG of different doses could significantly reduce the content of TC and TG in serum of hyperlipidemia rats,and increase the content of HDL;TC and TG in heart and liver were also decreased;besides,it could increase the content of SOD,CAT and GSH in the liver of hyperlipidemia rats,and reduce the content of MDA.The results of pathological section showed that TFAG could improve the damage degree of hepatocytes in hyperlipidemia rats,and the effect of high dose group was similar to that of Xuezhikang group.In general,TFAG has good antioxidant and reducing blood lipid effects,and can effectively inhibit liver steatosis.

Effect of crude-herb moxibustion on blood lipids in rats with dyslipidemia

Objective:To evaluate the lipid-regulating effect of crude-herb moxibustion on rats with dyslipidemia.Methods:Fifty-four SpragueeDawley rats were randomly divided into six groups with nine rats each.Control group rats were fed a normal diet,and bilateral acu-point Fenglong(equivalent to ST40 in humans)on the hind legs were covered with a placebo(general mucilage)for 2 hours each day.Model group rats were fed a high-lipid diet for 2 weeks.Therapy group rats were fed a high-lipid diet for 2 weeks and then administered crude-herb moxibustion at ST40 for 2 hours each day for the next 2 weeks.Prevention group rats were administered crude-herb moxibustion 2 hours a day for 2 weeks and then fed a high-lipid diet for the subsequent 2 weeks.Prevention/Therapy group rats were each administered crude-herb moxibustion at ST40 for 2 hours each day for 2 weeks,followed by a high-lipid diet for the next 2 weeks,and then crude-herb moxibustion again at ST40 for another 2 weeks.Simvastatin group rats were fed a high-lipid diet for 2 weeks and then treated with simvastatin for the next 2 weeks.Blood lipids,hepatosomatic indices(HSIs)and epididymal fat pad weights of all rats were examined.Results:Compared with the Model group,levels of total cholesterol(TC),glycerinate,lowdensity lipoprotein cholesterol(LDL-C)and very low-density lipoprotein cholesterol(VLDL-C)in plasma collected from the Therapy group,the Prevention/Therapy group,and the Simvastatin group were decreased.Moreover,compared with the Model group,HSIs in Therapy group rats were also decreased following administration of crude-herb moxibustion,but TC,TG,HDLC,LDL-C,and VLDL-C levels in the Prevention group were higher than those in the Model group.Conclusion:The results reveal that blood lipids and HSIs appear to be modulated by the effect of crude-herb moxibustion and suggest therapeutic strategies for the treatment of dyslipidemia.

Study on Intervention of Blood Sugar and Blood Lipid in Diabetic Mice by Abrus Cantoniensis and Armeniaca mume Sieb and Their Compatibility

Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.

Comparative effects of plant oils and trans-fat on blood lipid profiles and ischemic stroke in rats

Since plant oils are believed to be better than animal fats for cerebrovascular and cardiovascular diseases, the effects of various plant oils and trans-fat on blood lipid profiles and ischemic stroke were investigated. Sprague- Dawley rats were fed a diet containing the oils or trans-fat, and then body weights, blood lipids, and effects on brain infarction and physical dysfunction induced by middle cerebral artery occlusion （MCAO） were analyzed. All the oils and trans-fat, except perilla oil, significantly increased body fats and body weight gain. Sesame oil and trans-fat specifically increased blood cholesterols and triglycerides, respectively, while perilla oil decreased both cholesterols and triglycerides. Perilla oil not only attenuated cerebral infarction, but also restored locomotor activity and rota-rod performances of MCAO rats. It is suggested that perilla oil among oils and fats could be the first choice to reduce the risk of metabolic syndrome and ischemic stroke.

The Relationships between Erythrocyte Membrane n-6 to n-3 Polyunsaturated Fatty Acids Ratio and Blood Lipids and C-reactive Protein in Chinese Adults:An Observational Study

Objective To investigate the relationships between erythrocyte membrane n-6：n-3 PUFAs ratio and blood lipids and high sensitivity C-reactive protein （hs-CRP）. Methods The Chinese and a observational study consisted of a population-based cross-sectiona subsequent 1-year follow-up study of 171 subjects with the fasting cholesterol of 5.13-8.00 mmol/L. study of 456 plasma total Results In the cross-sectional analysis, plasma low-density lipoprotein cholesterol （LDL-c） had a significant and negative association with the erythrocyte membrane n-6：n-3 PUFAs ratio （P for trend=0.019） after adjusting for sex, age and total PUFA percentage. In the follow-up study, 171 subjects were categorized into quartiles by the changes of n-6：n-3 ratio in erythrocyte membrane （△=month 12-month 0）. In the top quartile whose ratios of n-6：n-3 increased by an average of 1.25 during the follow-up, the LDL-c-lowering extent was 3.3 times of that in the lowest quartile whose ratios of n-6：n-3 decreased by an average of 1.13 （-1.07 mmol/L v.s. -0.32 mmol/L）. The hsCRP decreased by 0.11 mg/dL in the lowest quartile while increasing by 0.10 mg/dL in the top quartile （P for difference=0.052）. Conclusion Our results suggested that the balance between n-6 and n-3 fatty acids may optimize the cardiovascular benefits from dietary PUFAs.

Effects of Zhibitai capsules on blood lipids and arterial elasticity

Objective To evaluate the effects of Zhibitai(ZBT) on blood lipids and arterial elasticity in patients with high cardiovascular risk.Methods Eighty three subjects with high risk factors of cardiovascular diseases were enrolled into the study and randomized to two groups,i.e.,the ZBT group(n =44,treated with ZBT 0.48 g twice per day) and the atorvastatin(ATO) group(n =39,treated with ATO 10 mg per day).The levels of fasting serum lipids including total cholesterol(TC),triglyceride(TG),high and low density lipoprotein cholesterol(HDL-C and LDL-C) and pulse wave velocity(PWV) were investigated before and eight weeks after treatment.Results The serum levels of TG,TC,LDL-C,femoral-ankle PWV(FAPWV) and carotid-radial PWV(CRPWV) were markedly decreased after the therapy with ZBT or ATO for 8 weeks(P <0.05).There was no significant alteration in HDL-C and carotid-femoral PWV(CFPWV) levels in both ZBT and ATO groups(P >0.05) after eight-week's treatment.Conclusion ZBT could reduce the levels of FAPWV and CRPWV,thus improve the elasticity of medium-sized peripheral muscular arteries in addition to its lipids lowering effect.The efficacy of ZBT is similar to that of ATO.

Yangxin Dawayimixike honey paste inhibits atherosclerosis in ApoE^(-/-)mice by attenuating blood lipids and exerting anti-inflammatory activity

Background:Yangxin Dawayimixike honey paste(YDHP)is a representative traditional Chinese medicine,and its main function is curing angina pectoris,palpitation and neurasthenia.However,it is unclear whether YDHP can suppress the development of atherosclerosis.The aim of this study was to validate the potential application of YDHP in atherosclerosis therapy and search for its potential mechanisms.Methods:Seven-week-old ApoE^(-/-)mice were randomly divided into a normal group fed a normal diet,an atherosclerosis model group fed a high-fat diet,YDHP groups fed a high-fat diet mixed with different doses of YDHP and positive control groups fed a high-fat diet mixed with atorvastatin or rosuvastatin.After feeding for 10 weeks,body weight,blood lipids,liver and kidney function indexes,serum inflammatory cytokines and atherosclerotic plaque areas were measured.Serum metabolic profiles were detected by an automatic biochemical analyser.Serum inflammatory cytokines were quantified by enzyme-linked immunosorbent assay kits.Atherosclerotic plaque areas were analysed using Oil Red O staining.Results:The YDHP(200,400 and 800 mg/kg/d)treated groups showed reduced serum levels of low density lipoprotein-cholesterol(P<0.05,when 200 or 400 mg/kg/d of YDHP group compared with the atherosclerosis model group;P<0.01,when 800 mg/kg/d of YDHP group compared with the atherosclerosis model group),total cholesterol(P<0.05,when 200 mg/kg/d of YDHP group compared with the atherosclerosis model group;P<0.01,when 400 or 800 mg/kg/d of YDHP group compared with the atherosclerosis model group)and triglyceride(P<0.01),however,elevated serum levels of high-density lipoprotein cholesterol(P<0.01)compared to the atherosclerosis model group.YDHP inhibited the area of atherosclerotic lesions.In addition,YDHP suppressed the levels of serum inflammatory cytokines such as interleukin 6(P<0.05,when 200 mg/kg/d of YDHP group compared with the atherosclerosis model group;P<0.01,when 400 or 800 mg/kg/d of YDHP group compared with the atherosclerosis model group)and tumor necrosis factorα(P<0.05,when 200 mg/kg/d of YDHP group compared with the atherosclerosis model group;P<0.01,when 400 or 800 mg/kg/d of YDHP group compared with the atherosclerosis model group).Conclusion:Our study demonstrated that YDHP showed considerable activity in alleviating the formation of atherosclerotic plaques in ApoE^(-/-)mice by reducing blood lipids and exerting anti-inflammatory activity.

Effect of Soufeng Qutan Chinese medicine on blood lipid and IL-1β in atherosclerotic mice with AopE gene knockout

Objective:To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis.Methods:Mice of six weeks old which were knocked out gene ApoE and fed with a high-fat diet for 13 weeks to establish a model of atherosclerotic unstable plaque,and they were randomly divided into the model group,the atorvastatin group and the low,medium,and high-dose Shoufeng Qutan groups.In addition,six weeks old C57BL/6J mice were set as the blank con?trol group. Both the blank control group and the model group were given 0.9% normal saline by gavage. The low,medium,and high-dose groups of Soufeng Qutangroup and the atorvastatin group were given different doses of Soufeng Qutan Chinese Medicine Decoction and atorvastatin. After 13 weeks,the mice were sacrificed,and the abdominal aortic blood vessels were observed by HE staining. Total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C). and high-density lipopro?tein cholesterol(HDL-C)content were detected by a biochemical automatic analyzer and IL-1β levels were detected by ELISA.Results:Compared with the blank control group,a large number of foam cells could be seen in the model group with obvious plaques;serum TC,TG,LDL-C,and IL-1β levels were significantly increased(P<0.05),and HDL-C level was markedly de?creased (P<0.05). Compared with the model group,the Soufeng Qutangroup at all doses and the atorvastatin group had a small amount of foam cells and reduced plaque area;serum TC,TG,LDL-C,and IL-1β levels were significantly decreased (P<0.05),and the level of HDL-C was significantly increased (P<0.05).Conclusion:Soufeng Qutan Chinesemedicine can inhibit the pro?gression of cellular inflammation,downregulate the expression of IL-1β,reduce plaques,and improve blood lipids. This may be one of the molecular mechanisms of Soufeng Qutan Chinese medicine in the early treatment of atherosclerosis.

Advances in the relationship between apolipoprotein gene polymorphism and blood lipid and cardiovascular disease

Objective： Dyslipidemia is a leading cause of cardiovascular disease. At present, studies have shown that theincidence of cardiovascular disease in our country increased year by year. According to WHO statistics in 2013, itshowed that about 17 million people worldwide die from coronary heart disease （CHD） every year. Currently, CHDis the first cause of death in western countries and the incidence of CHD also showed a trend of increasing. Inrecent years more experts and scholars at home and abroad found gene polymorphism is closely related tohigh-density lipoprotein cholesterol （HDLC） gene and triglyceride （TG） levels. Apolipoprotein （APO） gene is akind of popular polymorphic proteins, whose genetic polymorphisms is through the impact of lipid metabolism,and then closely related to cerebrovascular diseases. But the results are different in different populations and races,or even the opposite. Methods： This review will summarize the gene polymorphism loci of commonapolipoprotein-ApoA1, ApoA5, Apo B, ApoC3, ApoE, which is associated with lipid levels and cardiovasculardisease. Conclusion： It is important for us to get a further understand and prevent the occurrence and developmentof cardiovascular disease from gene level..

Epidemiologic surve of blood lipid level among 1401 residents aged≥60 years in rong-gui community

Objectives To investigate prevalence of blood lipid level among 1401 residents aged≥60 years in rong-gui community.Methods 1401 ones(579 males,822 females, (68.12±6.64)years Of total 1503 residents aged≥60 years in the 2 villages which were randomly exampled in rong-gui community were investigated.867 ones aged among 60-69 years(61.9%),430 ones aged among 70-79 years (30.7%),104 ones aged≥60 years(7.4%).Investigative items included age,sex,total cholesterol(TC),triglyceride (TG),body height,body weight,waist circumference(WC), hip circumference(HC),fasting plasma glucose(FG),blood pressure,history of past illness,body mass index(BMI), waist hip ratio(WHR),waist height ratio(WHtR).Results (l)Of total 1401 residents,Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male(P【0.01), The difference among three age groups were not significant, the combined prevalence of hyperlipemia and borderline hyperlipemia was 80.1%.(2)The FG、BMI、SBP、DBP、WC、WHR、WHtR of residents with hyperlipemia were higher than ones with normal blood lipid level(P【0.01).(3)By Pearson correlation analysis,we found that hyperlipemia was obviously correlated with FG,BMI,WC,WHR,WHtR, SBP,DBP.Conclusions(1)Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male.(2) hyperlipemia were obviously correlated with higher FG、BMI、WC、WHR、WHtR、SBP、DBP.

Effects of Danqi Yishen Capsule on Blood Lipid Levels and Hemorheological Indicators of Hyperlipidemia Patients

Objective: To explore the effects of Danqi Yishen Capsule (Danqi Capsule for Tonifying Kidney) on blood lipid levels and hemorheological indicators of hyperlipidemia patients. Methods: A total of 96 patients with hyperlipidemia were randomly divided into the control group and observation group of 48 cases respectively. The control group was given by simvastatin in oral administration of 10 mg half an hour after dinner, once a day, while the observation group based on the treatment of the control group, was orally given Danshen Yishen Capsule of three capsules each time, three times a day. The treatment of both groups lasted for two months. Then the comparison focused on blood lipid levels, hemorheological indicators, and adverse reactions between both groups before and after the treatment. Results: There was no significant difference in blood lipid levels and hemorheological indicators between both groups before the treatment (P > 0.05). The symptoms of both groups were significantly improved after the treatment, and the difference before and after the treatment was statistically significant (P < 0.05), and the difference between both groups was also statistically significant (P < 0.05). There was no significant difference in the incidence rate of adverse reactions between both groups (P > 0.05). Conclusion: Danqi Yishen Capsule has a significant value in clinical applications, which can optimize clinical efficacy, improve blood lipid levels and hemorheological indicators of hyperlipidemia patients, as well as reduce the probability of adverse reactions in patients.