High-throughput screening system of citrus bacterial cankerassociated transcription factors and its application to the regulation of citrus canker resistance

One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory network.To detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were developed.Screening the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this system.One TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis regulation.Electrophoretic mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional regulation.The CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2homeostasis.The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.In addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.

Systematic analysis and functional verification of citrus glutathione S-transferases reveals that CsGSTF1 and CsGSTU18contribute negatively to citrus bacterial canker

Citrus bacterial canker(CBC) is resulted from Xanthomonas citri subsp. citri(Xcc) infection and poses a significant threat to citrus production.Glutathione S-transferases(GSTs) are critical in maintaining redox homeostasis in plants, especially in relation to abiotic and biotic stress responses. However, the function of GSTs in resisting CBC remains unclear. Here, citrus glutathione S-transferases were investigated applying a genome-wide approach. In total, 69 CsGSTs belonging to seven classes were identified, and the phylogeny, chromosomal distribution, gene structures and conserved motifs were analyzed. Several CsGSTs responded to Xcc infection, as observed in the upregulation of CsGSTF1 and CsGSTU18 in the CBC-sensitive ‘Wanjincheng' variety but not in the resistant ‘Kumquat' variety. CsGSTF1 and CsGSTU18 were localized at the cytoplasm. Transient overexpression of CsGSTF1 and CsGSTU18 mediated reactive oxygen species(ROS) scavenging, whereas the virus-induced gene silencing(VIGS) of CsGSTF1 and CsGSTU18 caused strong CBC resistance and ROS burst. The present study investigated the characterization of citrus GST gene family, and discovered that CsGSTF1 and CsGSTU18 negatively contributed to CBC through modulating ROS homeostasis. These findings emphasize the significance of GSTs in infection resistance in plants.

国密SM4算法CBC模式的高效设计与实现

密码技术是现代信息安全技术产业发展的核心,其中,国密SM4分组密码算法因其硬件实现简单、效率高等优点,已广泛应用于加密传输、加密存储等领域.随着应用领域的不断扩展,对硬件加密效率的需求也随之提高.目前,借助流水线技术,基于ASIC实现的SM4算法在ECB(electronic code book)工作模式下能够达到较高的吞吐量.然而,在CBC(cipher block chaining)模式下,由于相邻的数据存在依赖关系,流水线技术难以提高硬件设计的吞吐率.为解决这一问题,提出了2种逻辑化简方法:一种作用于轮函数迭代过程,另一种作用于S盒置换过程.这2种方法在每一轮迭代的关键路径中均减少了2个异或运算的延时.在TSMC 40 nm工艺下的ASIC综合结果表明,该设计在CBC模式下的吞吐率达到4.2 Gb/s,单位面积吞吐量达129.4 Gb·s^(-1)·mm^(-2),高于已发表的同类设计.

Evaluation of Resistance of Different Kiwifruit Varieties (Lines) to Canker Disease and Brown Spot Disease

Kiwifruit canker and brown spot are significant diseases affecting kiwis,caused by Pseudomonas syringae patho-genic variations(Pseudomonas syringae pv.Actinidiae(Psa))and Corynesporapolytica(Corynespora cassiicola).At present,the research on canker disease and brown spot disease mainly focuses on the isolation and identification of pathogenic bacteria,drug control,resistance gene mining and functional verification.Practice has proved that breeding disease resistant varieties are an effective method to control canker disease and brown spot disease.However,most existing cultivars lack genes for canker and brown spot resistance.Wild kiwifruit resources in nat-ure exhibit extensive genetic diversity due to prolonged natural selection,containing numerous resistance genes.But,due to insufficient understanding of the resistance of most kiwifruit varieties(lines)to canker disease and brown spot disease,some high-quality resources have not been fully utilized.The incidence of canker and brown spot of 18 kiwifruit cultivars(lines)was measured by inoculating isolated branches and leaves,and their resistance to canker and brown spot was analyzed according to the length,disease index,mean diameter,and systematic clustering.The results were as follows:Among 18 different kiwifruit varieties(lines)for canker disease,there were two highly resistant materials,eight disease-resistant materials,four disease-susceptible materials,and two highly susceptible materials.Moreover,regarding brown spot disease,there were one highly resistant material,five dis-ease-resistant materials,four susceptible materials,and three highly susceptible materials.Furthermore,four resources were resistant to both diseases.The outcomes provided a theoretical basis for breeding kiwifruit against canker and brown spot.

An endolysin gene from Candidatus Liberibacter asiaticus confers dual resistance to huanglongbing and citrus canker

The most damaging citrus diseases are Huanglongbing(HLB)and citrus canker,which are caused by Candidatus Liberibacter asiaticus(CaLas)and Xanthomonas citri pv.citri(Xcc),respectively.Endolysins from bacteriophages are a possible option for disease resistance in plant breeding.Here,we report improvement of citrus resistance to HLB and citrus canker using the LasLYS1 and LasLYS2 endolysins from CaLas.LasLYS2 demonstrated bactericidal efficacy against several Rhizobiaceae bacteria and Xcc,according to inhibition zone analyses.The two genes,driven by a strong promoter from Cauliflower mosaic virus,35S,were integrated into Carrizo citrange via Agrobacterium-mediated transformation.More than 2 years of greenhouse testing indicated that LasLYS2 provided substantial and long-lasting resistance to HLB,allowing transgenic plants to retain low CaLas titers and no obvious symptoms while also clearing CaLas from infected plants in the long term.LasLYS2 transgenic plants with improved HLB resistance also showed resistance to Xcc,indicating that LasLYS2 had dual resistance to HLB and citrus canker.A microbiome study of transgenic plants revealed that the endolysins repressed Xanthomonadaceae and Rhizobiaceae populations in roots while increasing Burkholderiaceae and Rhodanobacteraceae populations,which might boost the citrus defense response,according to transcriptome analysis.We also found that Lyz domain 2 is the key bactericidal motif of LasLYS1 and LasLYS2.Four endolysins with potential resistance to HLB and citrus canker were found based on the structures of LasLYS1 and LasLYS2.Overall,the work shed light on the mechanisms of resistance of CaLas-derived endolysins,providing insights for designing endolysins to develop broad-spectrum disease resistance in citrus.

浙江省香椿溃疡病病原菌的分离和鉴定

[目的]分离和鉴定引起浙江省香椿溃疡病的病原菌,揭示香椿溃疡病的病因,为该病害的科学防控提供理论依据。[方法]观察香椿溃疡病的田间症状,使用常规组织分离法分离病原菌,利用形态学结合分子生物学方法对病原菌进行物种鉴定,采用柯赫氏法则验证分离菌株的致病性。[结果]1)从感病香椿样品中分离到2株病原菌;病原菌的培养性状、有性世代和无性世代的形态特征与假毛丛赤壳菌一致。2) LSU rDNA、ITS rDNA和tef1-α基因序列分析以及多基因系统发育分析表明,XCKY2菌株的LSU rDNA、ITS rDNA和tef1-α基因片段与假毛丛赤壳菌的同源性均为100.00%;XCKY1和XCKY2菌株与11株假毛丛赤壳菌聚为一支,贝叶斯后验概率为0.94。3)致病性测定结果表明,假毛丛赤壳菌的菌饼和分生孢子悬浮液均能引起健康香椿苗发病,从发病香椿苗组织中也能再分离到假毛丛赤壳菌,由此确定假毛丛赤壳菌为香椿溃疡病的病原菌。[结论]从浙江省感病香椿样品上分离到2株假毛丛赤壳菌,经鉴定为引起香椿溃疡病的病原菌。本研究首次报道假毛丛赤壳菌是香椿溃疡病病原菌。

基于CiteSpace的林木腐烂病研究现状与趋势分析

为全面系统了解林木腐烂病国内外研究现状和进展情况,检索Web of Science(WOS)核心集和中国知网(CNKI)1990—2022年收录的林木腐烂病相关研究文献,运用CiteSpace 5.8.R3软件对纳入文献的国家、机构、作者共现、关键词聚类及突现等数据进行可视化分析。经筛选,共纳入WOS文献621篇、CNKI文献1886篇。国际上林木腐烂病研究热度总体呈上升趋势,研究国家以中、美为主,研究机构以高校为主,国内机构和学者非常活跃,合作较多,国家之间合作不足。目前较活跃的高频关键词聚类为taxonomy(分类)、antifungal activity(抗真菌活性)、mycovirus(真菌病毒)和病虫害等。国际上林木腐烂病研究热点及趋势在维度上不断发展,聚焦bark(树皮)、purification(纯化)、lectin(凝集素)、diversity(多样性)等热点问题,国内研究集中于福美砷、木质部、树势、桥接等传统角度,并逐渐关注孢子角、发病规律、抑菌活性等主题的中国国情下的研究。

广西产区柑桔溃疡病和柑桔全爪螨同治降本增效技术研究

柑桔全爪螨(俗称红蜘蛛)和柑桔溃疡病是广西沃柑生产上防控用药量最大的两种病虫害。为探索同时防治这两种病虫害的综合技术方案,从而达到降本增效的目的,设计了99%矿物油乳油∶77%氢氧化铜可湿性粉剂∶80%代森锰锌可湿性粉剂=5∶1∶2混配的“油铜锰方案”,并且3—7月在田间开展丰田FT-18型背负式电动喷雾器人工喷雾、极目EA-30XP农业无人机常量飞防和推荐施药量减量30%飞防不同施药方式的对比试验。试验表明,采用“油锰铜方案”防治柑桔红蜘蛛和柑桔溃疡病时,常量飞防和减量飞防的防治效果不低于人工喷雾,有些时期甚至更好。“油锰铜方案”减量飞防对柑桔红蜘蛛的防治效果最高可达97.71%,对柑桔溃疡病的防治效果可达98.57%。“油锰铜方案”减量飞防可实现“一高三减”的效果,即每小时10×667 m^(2)的高施药工效、减少用水量94%、减少人工成本33.33%和减少农药使用量37.5%。“油锰铜方案”减量飞防能有效解决广西沃柑产区柑桔溃疡病、柑桔红蜘蛛“一病一虫”施药难、防治效果差和防治成本高的难题,可在地势平坦的柑桔产区大面积推广应用。

7种药剂对火龙果溃疡病田间防治药效试验

通过大田试验研究了锐收果香(400 g/L氯氟醚·吡唑酯悬浮剂)、健达(42.4%唑醚·氟酰胺悬浮剂)、碧翠(16%二氰·吡唑醚水分散粒剂)、善常(35%苯甲-吡唑酯悬浮剂)、阿米妙收(325 g/L苯甲·嘧菌酯悬浮剂)、露娜森(43%氟吡菌酰胺·肟菌酯悬浮剂)、拿敌稳(75%肟菌脂·戊唑醇水分散粒剂)7种药剂不同剂量对火龙果溃疡病(Neoscytalidium dimidiatum)的防治效果及其对火龙果产量的影响。研究结果表明:分别以有效成分锐收果香270 g/hm^(2)、健达360 g/hm^(2)、碧翠360 g/hm^(2)、善常270 g/hm^(2)、阿米妙收292.5 g/hm^(2)、露娜森193.5 g/hm^(2)、拿敌稳225 g/hm^(2),在火龙果生长旺盛期、溃疡病发生初期(开花初期),对火龙果茎叶全株喷药3次,每隔10 d 1次,7种药剂不同剂量在第3次药后14 d防效都在64%以上;其中,健达360 g/hm^(2)、阿米妙收292.5 g/hm^(2)、露娜森193.5 g/hm^(2)、拿敌稳225 g/hm^(2)防治效果超过80%,经过田间测产,健达360 g/hm^(2)、阿米妙收292.5 g/hm^(2)、露娜森193.5 g/hm^(2)、拿敌稳225 g/hm^(2)处理产量在7个处理中较高,4个处理的增产率均超过10%,综合整个试验的分析和观察,健达360 g/hm^(2)、阿米妙收292.5 g/hm^(2)、露娜森193.5 g/hm^(2)、拿敌稳225 g/hm^(2)4个处理,对火龙果溃疡病的防治效果最好。

延安地区防治苹果树腐烂病药剂调查及田间防效试验

为了解延安地区防治苹果腐烂病药剂种类,筛选防治苹果树腐烂病高效药剂,为苹果树腐烂病绿色防控提供依据。首先调查了目前延安地区常用防治腐烂病的药剂种类。在调查基础上,以29 a生富士苹果树为试验材料,采用病疤刮治法,选用5种杀菌剂对苹果树腐烂病进行田间防效试验,以病疤复发率、防治效果、愈合度和愈合宽度为指标对药效进行综合评价。试验结果表明,使用0.5%小蘖碱(溃腐灵)水剂原液、1.8%辛菌胺醋酸盐水剂200倍液、新敌腐2代原液、6%嘧啶核苷类抗菌素(农抗120)15倍液、3%甲基硫菌灵(国光膜泰)糊剂125 g/m^(2)涂抹刮净的病疤后,病斑复发率依次为3.4%、6.8%、3.0%、4.0%、0;愈合度依次为28.25%、18.52%、47.11%、20.13%、50.55%;愈伤组织平均宽度为2.97 cm、1.88 cm、3.56 cm、1.64 cm、4.73 cm;防治效果分别为96.6%、93.2%、97.0%、96.0%、100%,均高于90%。综合药效评价,5种杀菌剂对防治苹果树腐烂病都有效果,其中3%甲基硫菌灵糊剂125 g/m^(2)综合效果最好,其次是新敌腐2代原液和0.5%小蘖碱水剂原液。本试验5种药剂均可作为生产中防治腐烂病应用药剂。

山荆子MbLRK10L1.1基因正调控腐烂病抗性

此前从东北山荆子(Malus baccata)中发现可能参与腐烂病信号响应的WAK基因 MbLRK10L1.1,在此基础上,拟结合生物信息学分析、表达分析和功能验证研究其在腐烂病抗性中的作用机制。结果表明, MbLRK10L1.1响应Valsa mali(Vm)信号,并且在瞬时表达 MbLRK10L1.1后,‘烟富6号’果实表面病斑扩散速率明显减小, FRK1(Flg22-induced Receptor-like Kinase 1)、TaNOX(Triticum aestivum NADPH oxidase)、 EDS1(Enhanced disease susceptibility 1)等相关抗病基因均有不同程度的上调表达。表明 MbLRK10L1.1可通过JA、SA、SAR以及PTI等途径正调控果实对腐烂病菌的抗性。

CBC改性磷石膏基复合胶凝材料的制备与工艺研究

以磷石膏为主要原料,研究CBC改性磷石膏基复合胶凝材料的特性。结果表明,将磷石膏与SH按比例混合,陈化2 h,将陈化后的原料按比例加入磷渣、KZ、粉煤灰等浇注成型,脱模之后放入常压养护箱中养护24 h的材料性能最好。在此基础上,对不同加水量、成型方式下试件性能进行分析,优化了最佳的加水量和成型方式。经CBC改性石膏基复合胶凝材料兼具CBC和石膏性能,通过现有工艺技术的研究为磷石膏资源化提供了一种新的、切实可行的技术方法。

软枣猕猴桃新品种湘猕枣的选育

湘猕枣是从湖南浏阳市大围山野生软枣猕猴桃群体中选育出的新品种。果实呈长柱形,果肉绿色,成熟后果皮呈紫红色,种子附近果肉呈紫红色。果皮光滑无毛,表面蜡质具果粉。平均单果质量为25.18 g,最大单果质量为28.33g,果形指数为1.37。花后100d采收时的可溶性固形物含量(w,后同)为13.13%,总酸含量为1.13%,后熟期果实可溶性固形物含量最高可达26.5%,平均可溶性固形物含量为23.34%,干物质含量为22.28%。单个果实平均含有种子130粒,维生素C含量为36 mg·100 g^(-1),果柄长度为11.14~26.29 mm。该品种抗逆性强,溃疡病抗性强于米良1号,可作为鲜食和加工品种。

Investigation of Kiwifruit Bacterial Canker Disease(Pseudomonas syringae pv.Actinidiae Takikawa) in Guanzhong Area of Shanxi Province and Its Biological Control

[ Objective ] The paper was to grasp the incidence characteristic of kiwifruit bacterial canker disease （Pseudomonas syrirtgate pv. actinidiae Takikawa） in Guanzhong area of Shanxi province, and to screen the endophytic actinomycetes with control effect against kiwifruit bacterial canker. [ Method] The incidence characteristics of kiwifruit bacterial canker disease in 65 orchards of 13 villages in guanzhong area of Shanxi province （Yangling, Zhouzhi, Meixian） were investiga- ted and analyzed. The antagonistic strains against Pseudomonas syringae were simultaneously screened from 242 endophytic actinomycetes, and carried out field dis- ease control test. [ Result] The average incidence rate of kiwifruit bacterial canker disease in guan^ong area of Shanxi province was 7.95% ; the variety of kiwifruit had dose relationship with incidence rate. 76 strains bad inhibition activity agmnst P. syringae, while the inhibition zone diameters of fermentation broths of 35 strains against P. syr/ngae were greater than 20 nun. TIASA5 strain had inhibition activity against four target bacteria; gCLA4 strain not only had inhibition activity against four target bacteria, but also had strong inhibition activity against 10 species of plant pathogenic fungi. Field test showed that the fermentation broth of gCLA4 strain had obvious control effect against kiwifruit bacterial canker disease, and the relative control effect at 21 d could reach 66.7%. [ Conclusion] The an- tibacterial spectrum of fermentation broth of gCLA4 strain is broad, so gCLA4 strain has the potential to be developed into a broad-spectrum agricultural fungicide. Key words Kiwifruit bacterial canker disease; Endophytic actinomycetes; Biological control; Field test

CBC联合LDH对白血病、淋巴瘤骨髓侵犯诊断价值的ROC分析研究

目的探讨血细胞计数(CBC)联合乳酸脱氢酶(LDH)对白血病、淋巴瘤骨髓侵犯早期筛查的临床诊断价值,建立筛查规则。方法收集我院260例白血病、淋巴瘤骨髓侵犯患者初诊或复发时初次CBC、LDH结果,运用受试者工作曲线(ROC)分析白细胞总数(WBC)、血红蛋白(HB)、血小板(PLT)、淋巴细胞绝对值(L#)、淋巴细胞百分比(L%)、中性粒细胞绝对值(N#)、中性粒细胞百分比(N%)、单核细胞绝对值(M#)、单核细胞百分比(M%)及LDH对白血病、淋巴瘤骨髓侵犯诊断的曲线下面积(AUC),筛选出具有诊断价值的指标。分析这些指标联合诊断的灵敏度(SE)、特异性(SP)、阳性预测值(PPV)、阴性预测值(NPV),建立筛查规则。结果增高有诊断价值的指标有WBC、L#、L%、LDH、M#;减低有诊断价值的指标有N%、HB、PLT、N#。建立的CBC+LDH筛查规则如下:WBC≥20×109/L或≤2.7×109/L;HB≤60 g/L;PLT≤55×109/L或≥355×109/L;L#≥3.5×109/L;L%≥52%;M#≥0.7×109/L;LDH≥400U/L;WBC不能分类或分类不全;当WBC≥20×109/L,HB、PLT不减低,L#、L%、PLT不增高,不存在WBC不能分类或分类不全情况,则可排除白血病及淋巴瘤骨髓侵犯的可能性。该筛查规则对白血病、淋巴瘤骨髓侵犯的诊断灵敏度、特异性、阳性预测值、阴性预测值分别为95.4%、62.5%、80.5%、89.3%。结论 CBC+LDH法优于CBC法,可作为白血病、淋巴瘤细胞骨髓侵犯的有效筛查手段。

7种杀菌剂对3种林木腐烂病菌的毒力测定及活性评价

林木腐烂病是苹果树、梨树和杨树等林木枝干的重要真菌性病害。为了筛选出对苹果树腐烂病菌Valsa mali var.mali、梨树腐烂病菌V.mali var.pyri和杨树腐烂病菌V.sordida等3种不同寄主腐烂病菌都能有效防控的杀菌剂,本研究开展室内毒力试验比较了7种杀菌剂对3种腐烂病病原菌菌丝生长和分生孢子萌发的抑制效果,并进一步通过田间活性测定试验比较7种杀菌剂对梨树腐烂病病斑扩展和分生孢子发生的防治效果,同时测定了增效剂8.6%聚乙二醇(PEG)对7种杀菌剂的增效作用。毒力测定结果表明,苯醚甲环唑、戊唑醇、吡唑醚菌酯和丙唑·多菌灵对3种腐烂病病原菌菌丝生长和分生孢子萌发的抑制作用较强,其中EC_(50)平均值最低的是苯醚甲环唑,而戊唑醇的MIC平均值最低,在0.33 mg/L浓度下对3种腐烂病病原菌的菌丝生长和分生孢子萌发抑制率均达到100%。田间试验结果表明,45%苯醚甲环唑SC、43%戊唑醇SC和35%丙唑·多菌灵SE对梨树腐烂病病斑扩展和分生孢子萌发的防治效果突出,其中45%苯醚甲环唑SC 30.00 mg/L对病斑扩展防治效果达到82.23%,孢子萌发抑制效果达到85.96%,田间防治效果最好。10%丙硫唑SC+8.6%PEG处理组对病斑扩展防治效果提高了15.39百分点,达到73.46%,分生孢子萌发抑制率提高了23.75百分点,达到83.06%,增效作用显著。本研究为苹果树、梨树和杨树等3种寄主腐烂病的化学防控提供了科学依据。

BOC、MBOC和CBCS三种子载波调制方式的性能分析

根据自相关函数、功率谱密度、多径误差包络和Cramer Rao跟踪误差下边界等性能指标,分析和评估了BOC、MBOC和CBCS的性能。结果表明,MBOC和CBCS的功率谱密度的两个分裂主瓣在±1.023MHz附近,保证了与BOC(1,1)的互操作性,但它们的高频分量幅度都明显高于BOC(1,1);MBOC和CBCS的自相关函数比BOC(1,1)窄;从抗多径误差能力和Cramer Rao跟踪误差下边界这两方面来比较,MBOC和CBCS的性能比BOC(1,1)好。

杨树溃疡病拮抗菌防治机制研究进展

随着杨树栽植面积不断扩大,杨树溃疡病发生趋重,对树木造成严重损害。杨树溃疡病主要防治手段为化学药剂,但环境污染和病原菌耐药性等问题越发严重,需开发无污染且防治有效的技术或策略。以植物根际微生态组学为研究手段,可获得提升植物抗病能力的有益微生物。这些拮抗菌能够抑制溃疡病原菌的生长且无环境污染,具有很好的生物防治潜力。鉴于此,本综述主要探讨拮抗菌对防治杨树溃疡病的作用方式,包括通过养分水分竞争和分泌抑菌物质抑制病原菌生长,提高植物抗病性抵御病原菌侵染,以及提高宿主自身的活性氧耐受性等机制。未来利用多组学手段,将微生物培养与宏基因组学、代谢组学充分结合,进一步探究拮抗菌的协同作用机制,将有助于开发高效、稳定、环保的微生物菌剂;同时,寻找合适的纳米材料作为载体,构建能最大限度发挥拮抗菌功能的菌剂体系,也是未来生物控制剂研发的重要方向之一。

CBC技术在动态可重构CAPP系统中的应用

文章分析了目前ＣＡＰＰ系统普遍存在的问题，针对这些问题，提出了利用面向对象的方法对 ＣＡＰＰ系统进行分析和设计，利用基于 ＣＯＭ的组件（ＣＯＭ Ｂａｓｅｄ Ｃｏｍｐｏｎｅｎｔ；ＣＢＣ）技术对ＣＡＰＰ系统进行开发。并通过实例，详细论述了利用面向对象方法和组件技术对 ＣＡＰＰ系统进行分析、设计和开发的过程。

CaO-SiO_2-P_2O_5-H_2O 系统中 CBC 材料的原料配比研究

以硅溶胶、磷酸和硝酸钙为原料，以１８种不同化学成分的原料配比分别合成了ＣａＯ－ＳｉＯ２－Ｐ２Ｏ５－Ｈ２Ｏ系统中的ＣＢＣ材料．引入磷率（ＰＭ）或硅率（ＳＭ）及氧化钙标准值（ＣＳｔ）的概念，综合材料的化学成分及矿物组成的分析结果，以测得的劈裂抗拉强度指标为依据，对材料的配料问题进行研究．结果表明，欲制得高劈裂抗拉强度的材料，应控制ＰＭ为２０～３．０，ＣＳｔ为９０～１０５，或化学组成范围为：ＣａＯ，５３１～６１．２；ＳｉＯ２，９．３～１７．１；Ｐ２Ｏ５，２５．８～３５．５．