Background: Circus cyaneus is a medium-sized bird of prey that is widely distributed across the Northern Hemisphere. There are two currently recognized forms, the Palearctic form C. c. cyaneus(Hen Harrier), and the Ne...Background: Circus cyaneus is a medium-sized bird of prey that is widely distributed across the Northern Hemisphere. There are two currently recognized forms, the Palearctic form C. c. cyaneus(Hen Harrier), and the Nearctic form C. c. hudsonius(Northern Harrier). The forms have recently been split by the British Ornithologists' Union but the American Ornithologists' Union and some other taxonomic committees have not yet made any change. Here we examine the phylogenetic relationship between the two forms using sequence data from multiple nuclear and mitochondrial genes and examine breeding biology, body size, morphology, dispersal and other behaviors.Methods: In order to fully compare cyaneus and hudsonius, we carried out a full literature review, measured museum skins and carried out phylogenetic analysis using a number of different mitochondrial genes and compare our findings to other recent work.Results: We find that these two allopatric taxa form reciprocally monophyletic groups, show substantial mt DNA sequence divergence, and further differ significantly with respect to body size, plumage characters, breeding biology, dispersal and other behavioral traits.Conclusions: Based on an array of consistently divergent characteristics, it is suggested that the two forms are best regarded as separate species, Hen Harrier(Circus cyaneus) and Northern Harrier(Circus hudsonius).展开更多
AIM: To evaluate donation after circulatory death (DCD) orthotopic liver transplant outcomes [hypoxic cholangiopathy (HC) and patient/graft survival] and donor risk-conditions.METHODS: From 2003-2013, 45 DCD donor tra...AIM: To evaluate donation after circulatory death (DCD) orthotopic liver transplant outcomes [hypoxic cholangiopathy (HC) and patient/graft survival] and donor risk-conditions.METHODS: From 2003-2013, 45 DCD donor transplants were performed. Predonation physiologic data from UNOS DonorNet included preoperative systolic and diastolic blood pressure, heart rate, pH, SpO<sub>2</sub>, PaO<sub>2</sub>, FiO<sub>2</sub>, and hemoglobin. Mean arterial blood pressure was computed from the systolic and diastolic blood pressures. Donor preoperative arterial O<sub>2</sub> content was computed as [hemoglobin (gm/dL) × 1.37 (mL O<sub>2</sub>/gm) × SpO<sub>2</sub>%) + (0.003 × PaO<sub>2</sub>)]. The amount of preoperative donor red blood cell transfusions given and vasopressor use during the intensive care unit stay were documented. Donors who were transfused ≥ 1 unit of red-cells or received ≥ 2 vasopressors in the preoperative period were categorized as the red-cell/multi-pressor group. Following withdrawal of life support, donor ischemia time was computed as the number-of-minutes from onset of diastolic blood pressure < 60 mmHg until aortic cross clamping. Donor hypoxemia time was the number-of-minutes from onset of pulse oximetry < 80% until clamping. Donor hypoxia score was (ischemia time + hypoxemia time) ÷ donor preoperative hemoglobin.RESULTS: The 1, 3, and 5 year graft and patient survival rates were 83%, 77%, 60%; and 92%, 84%, and 72%, respectively. HC occurred in 49% with 16% requiring retransplant. HC occurred in donors with increased age (33.0 ± 10.6 years vs 25.6 ± 8.4 years, P = 0.014), less preoperative multiple vasopressors or red-cell transfusion (9.5% vs 54.6%, P = 0.002), lower preoperative hemoglobin (10.7 ± 2.2 gm/dL vs 12.3 ± 2.1 gm/dL, P = 0.017), lower preoperative arterial oxygen content (14.8 ± 2.8 mL O<sub>2</sub>/100 mL blood vs 16.8 ± 3.3 mL O<sub>2</sub>/100 mL blood, P = 0.049), greater hypoxia score >2.0 (69.6% vs 25.0%, P = 0.006), and increased preoperative mean arterial pressure (92.7 ± 16.2 mmHg vs 83.8 ± 18.5 mmHg, P = 0.10). HC was independently associated with age, multi-pressor/red-cell transfusion status, arterial oxygen content, hypoxia score, and mean arterial pressure (r<sup>2</sup> = 0.6197). The transplantation rate was greater for the later period with more liberal donor selection [era 2 (7.1/year)], compared to our early experience [era 1 (2.5/year)]. HC occurred in 63.0% during era 2 and in 29.4% during era 1 (P = 0.03). Era 2 donors had longer times for extubation-to-asystole (14.4 ± 4.7 m vs 9.3 ± 4.5 m, P = 0.001), ischemia (13.9 ± 5.9 m vs 9.7 ± 5.6 m, P = 0.03), and hypoxemia (16.0 ± 5.1 m vs 11.1 ± 6.7 m, P = 0.013) and a higher hypoxia score > 2.0 rate (73.1% vs 28.6%, P = 0.006).CONCLUSION: Easily measured donor indices, including a hypoxia score, provide an objective measure of DCD liver transplantation risk for recipient HC. Donor selection criteria influence HC rates.展开更多
We investigated the water-crossing behavior of Western Marsh Harriers(Circus aeruginosus),mostly adults,and European Honey Buzzards(Pernis apivorus),mostly juveniles,in relation to wind conditions,time of the day,floc...We investigated the water-crossing behavior of Western Marsh Harriers(Circus aeruginosus),mostly adults,and European Honey Buzzards(Pernis apivorus),mostly juveniles,in relation to wind conditions,time of the day,flocking and age classes,at a watchsite in central Italy during the autumn migration en route to Africa.Although European Honey Buzzards are less suited than Western Marsh Harriers to undertake long sea crossings,they were more inclined to leave the coast when migrating in flocks.Few birds of both species chose to fly along the coast.Western Marsh Harriers,such as European Honey Buzzards migrating alone,undertook the water crossing rather than stopping migration(birds roosting at the site or flying back inland) during the absence of wind and vice versa during head winds.Conversely,European Honey Buzzards migrating in flocks were not affected in their decision(crossing or stopping migration) by wind direction.Both species undertook the water crossing rather than stopping migration during mornings and vice versa during afternoons.Finally,in both species,adults and juveniles showed the same behavior in front of a water barrier.This result was expected in the case of the Western Marsh Harrier but not from the European Honey Buzzard since,in this species,the water-crossing tendency is age dependent with adults avoiding sea crossings.Our study confirms that flocking significantly affects the water-crossing behavior of European Honey Buzzards during migration.Moreover,in this species,inexperience of juveniles and presumably younger adults,about the high energetic costs of long powered flight and about the existence of shorter routes over water,might explain the strong water-crossing tendency shown by migrants independently from their age.展开更多
Pain is an unpleasant sensory and emotional experience associated with,or resembling that associated with,actual or potential tissue damage.The processing of pain involves complicated modulation at the levels of the p...Pain is an unpleasant sensory and emotional experience associated with,or resembling that associated with,actual or potential tissue damage.The processing of pain involves complicated modulation at the levels of the periphery,spinal cord,and brain.The pathogenesis of chronic pain is still not fully understood,which makes the clinical treatment challenging.Optogenetics,which combines optical and genetic technologies,can precisely intervene in the activity of specific groups of neurons and elements of the related circuits.Taking advantage of optogenetics,researchers have achieved a body of new findings that shed light on the cellular and circuit mechanisms of pain transmission,pain modulation,and chronic pain both in the periphery and the central nervous system.In this review,we summarize recent findings in pain research using optogenetic approaches and discuss their significance in understanding the pathogenesis of chronic pain.展开更多
The tropical Indian Ocean circulation system includes the equatorial and near-equatorial circulations, the marginal sea circulation, and eddies. The dynamic processes of these circulation systems show significant mult...The tropical Indian Ocean circulation system includes the equatorial and near-equatorial circulations, the marginal sea circulation, and eddies. The dynamic processes of these circulation systems show significant multi-scale variability associated with the Indian Monsoon and the Indian Ocean dipole. This paper summarizes the research progress over recent years on the tropical Indian Ocean circulation system based on the large-scale hydrological observations and numerical simulations by the South China Sea Institute of Oceanology(SCSIO), Chinese Academy of Sciences. Results show that:(1) the wind-driven Kelvin and Rossby waves and eastern boundary-reflected Rossby waves regulate the formation and evolution of the Equatorial Undercurrent and the Equatorial Intermediate Current;(2) the equatorial wind-driven dynamics are the main factor controlling the inter-annual variability of the thermocline in the eastern Indian Ocean upwelling;(3) the equatorial waves transport large amounts of energy into the Bay of Bengal in forms of coastal Kelvin and reflected free Rossby waves. Several unresolved issues within the tropical Indian Ocean are discussed:(i) the potential effects of the momentum balance and the basin resonance on the variability of the equatorial circulation system, and(ii) the potential contribution of wind-driven dynamics to the life cycle of the eastern Indian Ocean upwelling. This paper also briefly introduces the international Indian Ocean investigation project of the SCSIO, which will advance the study of the multi-scale variability of the tropical Indian Ocean circulation system, and provide a theoretical and data basis to support marine environmental security for the countries around the Maritime Silk Road.展开更多
The analysis of observed salinity data over 35 years (1961—1996) at four stations around the Bohai Sea, i.e. Huludao, Qinhuangdao, Tanggu and Beihuangcheng, reveals that the salinity of the 4 observation stations has...The analysis of observed salinity data over 35 years (1961—1996) at four stations around the Bohai Sea, i.e. Huludao, Qinhuangdao, Tanggu and Beihuangcheng, reveals that the salinity of the 4 observation stations has increased 1.1, 1.6, 1.9 and 0.4, respectively. The data also show that over the past 35 years, there have been at least 5 large salin-ity variation processes. The salinity data from two cruises of the Bohai Sea in August 1958 and 2000, show that the salin-ity pattern of the Bohai Sea has changed markedly. Low sa-linity in the sea surface layer around the old Yellow River mouth in August 1958 had been replaced by high salinity in August 2000 and the maximum variation of salinity is over 10.0. In addition, the values and distribution of salinity were almost the same from surface to bottom there in August 2000, but there existed significantly different salinity levels be-tween the surface layer and the deep layer in August 1958. When a comparison is made between the salinity levels of the above-mentioned two years, it is found that the salinity in August 2000 is on average 2.0 higher than that of August 1958 in the main part of the Bohai Sea. The change of tem-perature and salinity field in the Bohai Sea leads to the change of the circulation. The numerical simulation shows that in comparison with the circulation structure of the Bohai Sea in August 1958, the circulation in August 2000 changes markedly. The significant changes of circulation appeared in Bohai Bay, Laizhou Bay and in the middle of the Bohai Sea. The clockwise current loop outside of the Bohai Bay and counterclockwise current loop outside of the Laizhou Bay in August 1958 disappeared in August 2000, and the counterclockwise current loop of the Bohai Bay mi-grated obviously outward. The flow direction in the Laizhou Bay turned 180° around. Corresponding to the variation of the Bohai Sea circulation, the amount of water exchange between the Bohai Sea and the Yellow Sea has also changed. The water exchange rate through the Bohai Strait decreases, on average, 0.7×104 m3/s in August 2000 in contrast to that of August 1958.展开更多
基金The costs for the Cytochrome b sequencing and sample procurement was provided by the Museum of Vertebrate Zoology,UC Berkeley.Transport costs for museum visits were met by GJE
文摘Background: Circus cyaneus is a medium-sized bird of prey that is widely distributed across the Northern Hemisphere. There are two currently recognized forms, the Palearctic form C. c. cyaneus(Hen Harrier), and the Nearctic form C. c. hudsonius(Northern Harrier). The forms have recently been split by the British Ornithologists' Union but the American Ornithologists' Union and some other taxonomic committees have not yet made any change. Here we examine the phylogenetic relationship between the two forms using sequence data from multiple nuclear and mitochondrial genes and examine breeding biology, body size, morphology, dispersal and other behaviors.Methods: In order to fully compare cyaneus and hudsonius, we carried out a full literature review, measured museum skins and carried out phylogenetic analysis using a number of different mitochondrial genes and compare our findings to other recent work.Results: We find that these two allopatric taxa form reciprocally monophyletic groups, show substantial mt DNA sequence divergence, and further differ significantly with respect to body size, plumage characters, breeding biology, dispersal and other behavioral traits.Conclusions: Based on an array of consistently divergent characteristics, it is suggested that the two forms are best regarded as separate species, Hen Harrier(Circus cyaneus) and Northern Harrier(Circus hudsonius).
文摘AIM: To evaluate donation after circulatory death (DCD) orthotopic liver transplant outcomes [hypoxic cholangiopathy (HC) and patient/graft survival] and donor risk-conditions.METHODS: From 2003-2013, 45 DCD donor transplants were performed. Predonation physiologic data from UNOS DonorNet included preoperative systolic and diastolic blood pressure, heart rate, pH, SpO<sub>2</sub>, PaO<sub>2</sub>, FiO<sub>2</sub>, and hemoglobin. Mean arterial blood pressure was computed from the systolic and diastolic blood pressures. Donor preoperative arterial O<sub>2</sub> content was computed as [hemoglobin (gm/dL) × 1.37 (mL O<sub>2</sub>/gm) × SpO<sub>2</sub>%) + (0.003 × PaO<sub>2</sub>)]. The amount of preoperative donor red blood cell transfusions given and vasopressor use during the intensive care unit stay were documented. Donors who were transfused ≥ 1 unit of red-cells or received ≥ 2 vasopressors in the preoperative period were categorized as the red-cell/multi-pressor group. Following withdrawal of life support, donor ischemia time was computed as the number-of-minutes from onset of diastolic blood pressure < 60 mmHg until aortic cross clamping. Donor hypoxemia time was the number-of-minutes from onset of pulse oximetry < 80% until clamping. Donor hypoxia score was (ischemia time + hypoxemia time) ÷ donor preoperative hemoglobin.RESULTS: The 1, 3, and 5 year graft and patient survival rates were 83%, 77%, 60%; and 92%, 84%, and 72%, respectively. HC occurred in 49% with 16% requiring retransplant. HC occurred in donors with increased age (33.0 ± 10.6 years vs 25.6 ± 8.4 years, P = 0.014), less preoperative multiple vasopressors or red-cell transfusion (9.5% vs 54.6%, P = 0.002), lower preoperative hemoglobin (10.7 ± 2.2 gm/dL vs 12.3 ± 2.1 gm/dL, P = 0.017), lower preoperative arterial oxygen content (14.8 ± 2.8 mL O<sub>2</sub>/100 mL blood vs 16.8 ± 3.3 mL O<sub>2</sub>/100 mL blood, P = 0.049), greater hypoxia score >2.0 (69.6% vs 25.0%, P = 0.006), and increased preoperative mean arterial pressure (92.7 ± 16.2 mmHg vs 83.8 ± 18.5 mmHg, P = 0.10). HC was independently associated with age, multi-pressor/red-cell transfusion status, arterial oxygen content, hypoxia score, and mean arterial pressure (r<sup>2</sup> = 0.6197). The transplantation rate was greater for the later period with more liberal donor selection [era 2 (7.1/year)], compared to our early experience [era 1 (2.5/year)]. HC occurred in 63.0% during era 2 and in 29.4% during era 1 (P = 0.03). Era 2 donors had longer times for extubation-to-asystole (14.4 ± 4.7 m vs 9.3 ± 4.5 m, P = 0.001), ischemia (13.9 ± 5.9 m vs 9.7 ± 5.6 m, P = 0.03), and hypoxemia (16.0 ± 5.1 m vs 11.1 ± 6.7 m, P = 0.013) and a higher hypoxia score > 2.0 rate (73.1% vs 28.6%, P = 0.006).CONCLUSION: Easily measured donor indices, including a hypoxia score, provide an objective measure of DCD liver transplantation risk for recipient HC. Donor selection criteria influence HC rates.
文摘We investigated the water-crossing behavior of Western Marsh Harriers(Circus aeruginosus),mostly adults,and European Honey Buzzards(Pernis apivorus),mostly juveniles,in relation to wind conditions,time of the day,flocking and age classes,at a watchsite in central Italy during the autumn migration en route to Africa.Although European Honey Buzzards are less suited than Western Marsh Harriers to undertake long sea crossings,they were more inclined to leave the coast when migrating in flocks.Few birds of both species chose to fly along the coast.Western Marsh Harriers,such as European Honey Buzzards migrating alone,undertook the water crossing rather than stopping migration(birds roosting at the site or flying back inland) during the absence of wind and vice versa during head winds.Conversely,European Honey Buzzards migrating in flocks were not affected in their decision(crossing or stopping migration) by wind direction.Both species undertook the water crossing rather than stopping migration during mornings and vice versa during afternoons.Finally,in both species,adults and juveniles showed the same behavior in front of a water barrier.This result was expected in the case of the Western Marsh Harrier but not from the European Honey Buzzard since,in this species,the water-crossing tendency is age dependent with adults avoiding sea crossings.Our study confirms that flocking significantly affects the water-crossing behavior of European Honey Buzzards during migration.Moreover,in this species,inexperience of juveniles and presumably younger adults,about the high energetic costs of long powered flight and about the existence of shorter routes over water,might explain the strong water-crossing tendency shown by migrants independently from their age.
基金supported by grants from the National Natural Science Foundation of China(82073819 and 81872843)Fundamental Research Funds for the Central Universities of China(2021QNA7005).
文摘Pain is an unpleasant sensory and emotional experience associated with,or resembling that associated with,actual or potential tissue damage.The processing of pain involves complicated modulation at the levels of the periphery,spinal cord,and brain.The pathogenesis of chronic pain is still not fully understood,which makes the clinical treatment challenging.Optogenetics,which combines optical and genetic technologies,can precisely intervene in the activity of specific groups of neurons and elements of the related circuits.Taking advantage of optogenetics,researchers have achieved a body of new findings that shed light on the cellular and circuit mechanisms of pain transmission,pain modulation,and chronic pain both in the periphery and the central nervous system.In this review,we summarize recent findings in pain research using optogenetic approaches and discuss their significance in understanding the pathogenesis of chronic pain.
基金supported by the National Key Research and Development Program of China(Grant No.2017YFC1405100)the National Natural Science Foundation of China(Grant Nos.41521005,41476011,41706027,41676013)+4 种基金the Natural Science Foundation of Guangdong(Grant No.2016A030310015)the Open Fund of the Key Laboratory of Ocean Circulation and Waves,Chinese Academy of Sciences(Grant No.KLOCW1604)the Open Fund of the State Key Laboratory of Tropical Oceanography(Grant No.LTOZZ1702)the MEL Visiting Fellowship(Grant No.MELRS1640)the Guangzhou Science and Technology Foundation(Grant No.201804010133)
文摘The tropical Indian Ocean circulation system includes the equatorial and near-equatorial circulations, the marginal sea circulation, and eddies. The dynamic processes of these circulation systems show significant multi-scale variability associated with the Indian Monsoon and the Indian Ocean dipole. This paper summarizes the research progress over recent years on the tropical Indian Ocean circulation system based on the large-scale hydrological observations and numerical simulations by the South China Sea Institute of Oceanology(SCSIO), Chinese Academy of Sciences. Results show that:(1) the wind-driven Kelvin and Rossby waves and eastern boundary-reflected Rossby waves regulate the formation and evolution of the Equatorial Undercurrent and the Equatorial Intermediate Current;(2) the equatorial wind-driven dynamics are the main factor controlling the inter-annual variability of the thermocline in the eastern Indian Ocean upwelling;(3) the equatorial waves transport large amounts of energy into the Bay of Bengal in forms of coastal Kelvin and reflected free Rossby waves. Several unresolved issues within the tropical Indian Ocean are discussed:(i) the potential effects of the momentum balance and the basin resonance on the variability of the equatorial circulation system, and(ii) the potential contribution of wind-driven dynamics to the life cycle of the eastern Indian Ocean upwelling. This paper also briefly introduces the international Indian Ocean investigation project of the SCSIO, which will advance the study of the multi-scale variability of the tropical Indian Ocean circulation system, and provide a theoretical and data basis to support marine environmental security for the countries around the Maritime Silk Road.
文摘The analysis of observed salinity data over 35 years (1961—1996) at four stations around the Bohai Sea, i.e. Huludao, Qinhuangdao, Tanggu and Beihuangcheng, reveals that the salinity of the 4 observation stations has increased 1.1, 1.6, 1.9 and 0.4, respectively. The data also show that over the past 35 years, there have been at least 5 large salin-ity variation processes. The salinity data from two cruises of the Bohai Sea in August 1958 and 2000, show that the salin-ity pattern of the Bohai Sea has changed markedly. Low sa-linity in the sea surface layer around the old Yellow River mouth in August 1958 had been replaced by high salinity in August 2000 and the maximum variation of salinity is over 10.0. In addition, the values and distribution of salinity were almost the same from surface to bottom there in August 2000, but there existed significantly different salinity levels be-tween the surface layer and the deep layer in August 1958. When a comparison is made between the salinity levels of the above-mentioned two years, it is found that the salinity in August 2000 is on average 2.0 higher than that of August 1958 in the main part of the Bohai Sea. The change of tem-perature and salinity field in the Bohai Sea leads to the change of the circulation. The numerical simulation shows that in comparison with the circulation structure of the Bohai Sea in August 1958, the circulation in August 2000 changes markedly. The significant changes of circulation appeared in Bohai Bay, Laizhou Bay and in the middle of the Bohai Sea. The clockwise current loop outside of the Bohai Bay and counterclockwise current loop outside of the Laizhou Bay in August 1958 disappeared in August 2000, and the counterclockwise current loop of the Bohai Bay mi-grated obviously outward. The flow direction in the Laizhou Bay turned 180° around. Corresponding to the variation of the Bohai Sea circulation, the amount of water exchange between the Bohai Sea and the Yellow Sea has also changed. The water exchange rate through the Bohai Strait decreases, on average, 0.7×104 m3/s in August 2000 in contrast to that of August 1958.
