A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodict...A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.展开更多
A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation....A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared high sequence homology with viscotoxins isolated from Viscum album. Pharmacological experiments showed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could be used as a leading compound in cancer therapy.展开更多
Viscum coloratum(Kom.)Nakai is a well-known medicinal hemiparasite widely distributed in Asia.The synthesis and accumulation of its metabolites are affected by both environmental factors and the host plants,while the ...Viscum coloratum(Kom.)Nakai is a well-known medicinal hemiparasite widely distributed in Asia.The synthesis and accumulation of its metabolites are affected by both environmental factors and the host plants,while the latter of which is usually overlooked.The purpose of this study was to comprehensively evaluate the effects of host and habitat on the metabolites in V.coloratum through multiple chemical and biological approaches.The metabolite profile of V.coloratum harvested from three different host plants in two habitats were determined by multiple chemical methods including high-performance liquid chromatography-ultraviolet(HPLC-UV),gas chromatography-flame ionization detector(GC-FID)and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF/MS).The differences in antioxidant efficacy of V.coloratum were determined based on multiple in vitro models.The multivariate statistical analysis and data fusion strategy were applied to analyze the differences in metabolite profile and antioxidant activity of V.coloratum.Results indicated that the metabolite profile obtained by various chemical approaches was simultaneously affected by host and environment factors,and the environment plays a key role.Meanwhile,three main differential metabolites between two environment groups were identified.The results of antioxidant assay indicated that the environment has greater effects on the biological activity of V.coloratum than the host.Therefore,we conclude that the integration of various chemical and biological approaches combined with multivariate statistical and data fusion analysis,which can determine the influences of host plant and habitat on the metabolites,is a powerful strategy to control the quality of semi-parasitic herbal medicine.展开更多
In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially lig...In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.展开更多
The purpose of this study was to evaluate the similarity of chemical profile of Viscum loloratum harvested in different months using high performance liquid chromatography(HPLC)fingerprint and multivariate statistical...The purpose of this study was to evaluate the similarity of chemical profile of Viscum loloratum harvested in different months using high performance liquid chromatography(HPLC)fingerprint and multivariate statistical analysis,and to determine the optimal harvest time of Viscum coloratum.8 compounds were isolated from Viscum coloratum by a variety of isolation techniques,3 of which were isolated from the genus Viscum L.for the first time.The fingerprint of Viscum coloratum was established by HPLC,and 16 common peaks were obtained,4 of which were identified by comparison with the reference substances.Results showed that the Viscum coloratum harvested in 6 months could be roughly divided into three groups based on the chemical profiles,and the Viscum coloratum harvested in January had higher levels of the marked compounds.The HPLC fingerprint objectively reveals the characteristics of Viscum coloratum harvested in different months,which can be used to evaluate and control the quality of Viscum coloratum harvested in different months.展开更多
Mistletoes are parasite plants growing on various tree species,taking nutrients from the host.Beneficial compounds of mistletoe were used in medicine and these mistletoes were commonly collected from oak species that ...Mistletoes are parasite plants growing on various tree species,taking nutrients from the host.Beneficial compounds of mistletoe were used in medicine and these mistletoes were commonly collected from oak species that were not well identified.We analyzed the nutritional value by measuring proximate and mineral components of Korean mistletoe(Viscum album var.coloratum)from various host trees.Carbohydrate was the most proximate component in both leaves and twigs followed crude protein,crude fiber,crude ash and crude fat.While the contents of the proximate components and minerals varied among hosts and between plant parts,leaves contained higher crude protein and ash,and more minerals except for magnesium than twigs,although no significant difference was found inmistletoe grown on Prunus mandshurica.There were significant differences of proximate and mineral contents depending on the host species.Mistletoe grown on Chaenomeles sinensis contained higher levels of carbohydrates,crude fat,crude ash,magnesium,calcium,sodium,iron,and zinc than mistletoe grown on other species.Mistletoe grown on P.mandshurica contained high mineral levels of potassium,copper and phosphorous.The nutritional value of mistletoes were greatly influenced by the host species but among the hosts,specific nutrients accumulated in leaves more than in twigs,and vice versa.Based on the analysis,Korean mistletoe contains relatively good quality of nutrients and has high potential for dietary supplements as well as for medicinal compounds.展开更多
Viscum coloratum(Kom.)Nakai is a well-known medicinal plant.However,the optimal harvest time for V.coloratum is unknown.Few studies were performed to analyze compound variation during storage and to improve post-harve...Viscum coloratum(Kom.)Nakai is a well-known medicinal plant.However,the optimal harvest time for V.coloratum is unknown.Few studies were performed to analyze compound variation during storage and to improve post-harvest quality control.Our study aimed to comprehensively evaluate the quality of V.coloratum in different growth stages,and determine the dynamic variation of metabolites.Ultra-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in V.coloratum harvested in six growth periods,and the associated biosynthetic pathways were explored.The accumulation of different types of compounds were analyzed based on their synthesis pathways.Grey relational analysis was used to evaluate the quality of v.coloratum across different months.The compound variation during storage was analyzed by a high-temperature high-humidity accelerated test.The results showed that the quality of V.coloratum was the hightest in March,followed by November,and became the lowest in July.During storage,compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids,leading to an increase followed by a decrease in the content of some compounds,and resulted in a large gap during the degradation time course among different compounds.Due to the rapid rate and large degree of degradation,five compounds were tentatively designated as“early warning components”for quality control.This report provides reference for better understanding the biosynthesis and degradation of metabolites in V.coloratum and lays a theoretical foundation for rational application of V.coloratum and better quality control of V.coloratum during storage.展开更多
基金supported by the National NaturalScience Foundation of China(No.30901967)the Scientific Research Foundation for the Doctors of the Liaoning Provincial Department of Science and Technology(No.20091078)the Science and Technology Research Projects of the Educational Commission of Liaoning Province(No.2009A684)for financial support
文摘A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.
文摘A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared high sequence homology with viscotoxins isolated from Viscum album. Pharmacological experiments showed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could be used as a leading compound in cancer therapy.
基金funded by the National Natural Science Foundation of China(Grant No.:30901967)the Natural Science Foundation of Liaoning Province(Grant No.:2013020223)Shenyang Pharmaceutical University Student Science and Technology Innovation Project(Grant No.:12)。
文摘Viscum coloratum(Kom.)Nakai is a well-known medicinal hemiparasite widely distributed in Asia.The synthesis and accumulation of its metabolites are affected by both environmental factors and the host plants,while the latter of which is usually overlooked.The purpose of this study was to comprehensively evaluate the effects of host and habitat on the metabolites in V.coloratum through multiple chemical and biological approaches.The metabolite profile of V.coloratum harvested from three different host plants in two habitats were determined by multiple chemical methods including high-performance liquid chromatography-ultraviolet(HPLC-UV),gas chromatography-flame ionization detector(GC-FID)and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF/MS).The differences in antioxidant efficacy of V.coloratum were determined based on multiple in vitro models.The multivariate statistical analysis and data fusion strategy were applied to analyze the differences in metabolite profile and antioxidant activity of V.coloratum.Results indicated that the metabolite profile obtained by various chemical approaches was simultaneously affected by host and environment factors,and the environment plays a key role.Meanwhile,three main differential metabolites between two environment groups were identified.The results of antioxidant assay indicated that the environment has greater effects on the biological activity of V.coloratum than the host.Therefore,we conclude that the integration of various chemical and biological approaches combined with multivariate statistical and data fusion analysis,which can determine the influences of host plant and habitat on the metabolites,is a powerful strategy to control the quality of semi-parasitic herbal medicine.
基金supported by the forestry public welfare industry research special-study group on cultivation and utilization of medicinal fungi and astragalus in Daxing’anling(201004079)
文摘In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.
基金This work was supported by the Natural Science Foundation of Liaoning Province(No.20180551065).
文摘The purpose of this study was to evaluate the similarity of chemical profile of Viscum loloratum harvested in different months using high performance liquid chromatography(HPLC)fingerprint and multivariate statistical analysis,and to determine the optimal harvest time of Viscum coloratum.8 compounds were isolated from Viscum coloratum by a variety of isolation techniques,3 of which were isolated from the genus Viscum L.for the first time.The fingerprint of Viscum coloratum was established by HPLC,and 16 common peaks were obtained,4 of which were identified by comparison with the reference substances.Results showed that the Viscum coloratum harvested in 6 months could be roughly divided into three groups based on the chemical profiles,and the Viscum coloratum harvested in January had higher levels of the marked compounds.The HPLC fingerprint objectively reveals the characteristics of Viscum coloratum harvested in different months,which can be used to evaluate and control the quality of Viscum coloratum harvested in different months.
基金supported by Kangwon National University and NRF,Korea(No.2017R1D1A3B03032994)
文摘Mistletoes are parasite plants growing on various tree species,taking nutrients from the host.Beneficial compounds of mistletoe were used in medicine and these mistletoes were commonly collected from oak species that were not well identified.We analyzed the nutritional value by measuring proximate and mineral components of Korean mistletoe(Viscum album var.coloratum)from various host trees.Carbohydrate was the most proximate component in both leaves and twigs followed crude protein,crude fiber,crude ash and crude fat.While the contents of the proximate components and minerals varied among hosts and between plant parts,leaves contained higher crude protein and ash,and more minerals except for magnesium than twigs,although no significant difference was found inmistletoe grown on Prunus mandshurica.There were significant differences of proximate and mineral contents depending on the host species.Mistletoe grown on Chaenomeles sinensis contained higher levels of carbohydrates,crude fat,crude ash,magnesium,calcium,sodium,iron,and zinc than mistletoe grown on other species.Mistletoe grown on P.mandshurica contained high mineral levels of potassium,copper and phosphorous.The nutritional value of mistletoes were greatly influenced by the host species but among the hosts,specific nutrients accumulated in leaves more than in twigs,and vice versa.Based on the analysis,Korean mistletoe contains relatively good quality of nutrients and has high potential for dietary supplements as well as for medicinal compounds.
基金supported by Liaoning Revitalization Talents Program(No.XLYC1908031)。
文摘Viscum coloratum(Kom.)Nakai is a well-known medicinal plant.However,the optimal harvest time for V.coloratum is unknown.Few studies were performed to analyze compound variation during storage and to improve post-harvest quality control.Our study aimed to comprehensively evaluate the quality of V.coloratum in different growth stages,and determine the dynamic variation of metabolites.Ultra-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in V.coloratum harvested in six growth periods,and the associated biosynthetic pathways were explored.The accumulation of different types of compounds were analyzed based on their synthesis pathways.Grey relational analysis was used to evaluate the quality of v.coloratum across different months.The compound variation during storage was analyzed by a high-temperature high-humidity accelerated test.The results showed that the quality of V.coloratum was the hightest in March,followed by November,and became the lowest in July.During storage,compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids,leading to an increase followed by a decrease in the content of some compounds,and resulted in a large gap during the degradation time course among different compounds.Due to the rapid rate and large degree of degradation,five compounds were tentatively designated as“early warning components”for quality control.This report provides reference for better understanding the biosynthesis and degradation of metabolites in V.coloratum and lays a theoretical foundation for rational application of V.coloratum and better quality control of V.coloratum during storage.
