Coupling of reduced inorganic fertilizer with plant-based organic fertilizer as a promising fertilizer management strategy for colored rice in tropical regions

Colored rice is a type of high-quality,high-added-value rice that has attracted increasing attention in recent years.The use of large amounts of inorganic nitrogen fertilizer in rice fields results in low fertilizer use efficiency and high environmental pollution.Organic fertilizer is a promising way to improve soil quality and sustain high yields.However,most studies focus on the effect of animal-based organic fertilizers.The effects of different ratios of plantbased organic fertilizer and inorganic fertilizer on the grain yield and quality of colored rice have rarely been reported.Therefore,a two-year field experiment was conducted in 2020 and 2021 to study the effects of replacing inorganic N fertilizers with plant-based organic fertilizers on the yield,nitrogen use efficiency(NUE),and anthocyanin content of two colored rice varieties in a tropical region in China.The experimental treatments included no nitrogen fertilization(T1),100% inorganic nitrogen fertilizer(T2),30%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T3),60%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T4),and 100% plantbased organic fertilizer(T5).The total nitrogen provided to all the treatments except T1 was the same at 120 kg ha-1.Our results showed that the T3 treatment enhanced the grain yield and anthocyanin content of colored rice by increasing nitrogen use efficiency compared with T2.On average,grain yields were increased by 9 and 8%,while the anthocyanin content increased by 16 and 10% in the two colored rice varieties under T3 across the two years,respectively,as compared with T2.Further study of the residual effect of partial substitution of inorganic fertilizers showed that the substitution of inorganic fertilizer with plant-based organic fertilizer improved the soil physiochemical properties,and thus increased the rice grain yield,in the subsequent seasons.The highest grain yield of the subsequent rice crop was observed under the T5 treatment.Our results suggested that the application of plantbased organic fertilizers can sustain the production of colored rice with high anthocyanin content in tropical regions,which is beneficial in reconciling the relationship between rice production and environmental protection.

Vividly Colored Accessories and Mood Changes in Patients Undergoing Breast Surgery

Few studies have examined the impacts of color stimuli on perioperative mood and quality of recovery;thus, this randomized controlled trial aimed to assess impacts of vividly colored accessories on mood and quality of recovery after breast surgery. This single-center, single-blind randomized controlled trial included 36 participants (all aged ≥ 20 years) who were randomized into intervention (n = 19) and control groups (n = 17). The intervention group received vividly colored accessories. The primary and secondary study outcomes were patient mood, evaluated using a two-dimensional mood scale, and postoperative recovery, evaluated using Quality of Recovery-15, which were assessed on postoperative day 3. There were no statistical intergroup differences in the scores of the Two-Dimensional Mood Scale (11.2 [intervention group] vs. 10.4 [control group], P = 0.75) and Quality of Recovery-15 (126.8 [intervention group] vs. 129.3 [control group], P = 0.73). Thus, the use of vividly colored accessories by patients undergoing breast surgery was not found to affect patients’ mood and quality of recovery.

Identification of Shell Color-Associated Genes Using Mantle Branch-Specific RNA Sequencing of Yellow-Colored Line of Pearl Oyster Pinctada fucata martensii

The yellow-colored line of pearl oyster Pinctada fucata martensii displays a yellow prismatic layer and a white nacreous layer that can be used as an ideal model for research on shell color formation.Micro-Raman spectroscopy and transcriptome analyses were performed to explore the potential molecular mechanism underlying the phenotype differentiation.The micro-Raman spectroscopy results indicate that the prismatic layer exhibits distinct characteristic peaks of carotenoids,while these peaks are not prominent in the nacreous layer.In the transcriptome comparison of the central zone of mantle and mantle edge tissue,which function in nacreous and prismatic layer formation,respectively,935 significantly differentially expressed genes(DEGs)were identified,with 385 genes upregulated and 227 genes downregulated(|log_(2)(Fold change)|>1 and false discovery rate<0.05)in the mantle edge tissue.Among these genes,some were associated with melanoma/melanogenesis,such as tyrosinase,zinc metalloprotease,glutathione S-transferase,and ATP-binding cassette sub-family;some were associated with the carotenoid-related pathway,including scavenger receptors,cytochrome P450 and lipoprotein receptor.Genes associated with porphyrin metabolism,including porphobilinogen deaminase,and copper/zinc superoxide dismutase,and genes associated with shell matrix protein,including amorphous calcium carbonate binding protein,shematrin,PIF,and collagen,also exhibited significantly different expressions.It is speculated that the different colours between prismatic layer and nacreous layer in the yellow-colored line of P.f.martensii might be resulted from melanin,carotenoids and porphyrin metabolism,while genes related to shell structure and biomineralization might also affect coloration.Our results provide new insights to understand the mechanism of shell color formation in mollusca.

Flavanone and flavonoid hydroxylase genes regulate fiber color formation in naturally colored cotton

Using naturally colored cotton(NCC)can eliminate dyeing,printing and industrial processing,and reduce sewage discharge and energy consumption.Proanthocyanidins(PAs),the primary coloration components in brown fibers,are polyphenols formed by oligomers or polymers of flavan-3-ol units derived from anthocyanidins.Three essential structural genes for flavanone and flavonoid hydroxylation encoding flavanone-3-hydroxylase(F3H),flavonoid 3’-hydroxylase(F3’H)and flavonoid 3’5’-hydroxylase(F3’5’H)are initially committed in the flavonoid biosynthesis pathway to produce common precursors.The three genes were all expressed predominantly in developing fibers of NCCs,and their expression patterns varied temporally and spatially among NCC varieties.In GhF3Hi,GhF3’Hi and GhF3’5’Hi silenced lines of NCC varieties XC20 and ZX1,the expression level of the three genes decreased in developing cotton fiber,negatively correlated with anthocyanidin content and fiber color depth.Fiber color depth and type in RNAi lines changed with endogenous gene silencing efficiency and expression pattern,the three hydroxylase genes functioned in fiber color formation.GhF3H showed functional differentiation among NCC varieties and GhF3’H acted in the accumulation of anthocyanin in fiber.Compared with GhF3’H,GhF3’5’H was expressed more highly in brown fiber with a longer duration of expression and caused lighter color of fibers in GhF3’5’H silenced lines.These three genes regulating fiber color depth and type could be used to improve these traits by genetic manipulation.

Spatiotemporal characteristics and remote sensing estimation of colored dissolved organic matter around the Leizhou Peninsula

Colored dissolved organic matter(CDOM)is an important optically active substance in marine environment.Its biochemical conservatism makes it an important indicator to offshore pollution process.Monitoring the content,composition,and diffusion process of CDOM is a good approach to analyze the terrestrial input,optical properties,and ecological environment of offshore areas.The spatiotemporal characteristics of CDOM around the Leizhou Peninsula were analyzed based on field observation data collected in the autumn 2020 and spring 2021,and an empirical inversion model of the ag(355)(absorption coefficient at 355 nm)and spectral slope(Sg)(g stands for gelbstoff/gilvin,which is called CDOM)based on Sentinel-3A ocean and land color instrument(OLCI)images was constructed.The results show(1)the order of average ag(355)value around the Leizhou Peninsula was east coast(0.503/m)>Qiongzhou Strait(0.502/m)>west coast(0.365/m);(2)the best band combinations of CDOM inversion in spring and autumn were(B4+B11)/B3 and(B7-B1)/B6(B stands for the band of spectral images),and the final inversion results are close to the measured results,indicating that the model has good accuracy;(3)the S_(g)value of the CDOM absorption spectrum was fitted to the hyperbolicexponential model.The fitting accuracy of the model was higher than those of the exponential model and the hyperbolic model,and the best S_(g)inversion model was constructed by selecting Sg(275-295)and Sg(250-295)in spring and autumn;(4)the spatial distributions of ag(355)and S_(g)were inverted,and CDOM in the waters around the Leizhou Peninsula originated from terrestrial organic matter carried by coastal aquaculture zones and runoff in the northeast Zhanjiang and the northern Beibu Gulf.

Seasonal Distribution of Observed Colored Dissolved Organic Matter(CDOM)in the Eastern Indian Ocean

Colored dissolved organic matter(CDOM)is a crucial constituent that affects the optical absorption properties of seawater.Owing to the relatively limited measured data on the spatial distribution characteristics of CDOM in the tropical eastern Indian Ocean,this study analyzes the optical absorption characteristics of CDOM in the southeast Indian Ocean using the data collected during four seasons from 2013 to 2017.This work also systematically describes the seasonal horizontal and vertical distribution characteristics of CDOM in this area and conducts a preliminary analysis of the relevant factors affecting CDOM absorption characteristics in this region.Results indicate that the CDOM ag(440)during summer was remarkably lower than that in the coastal waters of Europe and coastal waters of China but slightly higher than that in the western and southeast Pacific.The spatial distribution of surface CDOM shows remarkable seasonal differences,and the spatial distribution characteristics of CDOM in the 5°S,92°E region differ between spring/summer and autumn/winter.The values of ag(400)and ag(440)are weak/strong at a surface/subsurface level of 100 m,with differences found between summer and winter.The correlation of CDOM with temperature,salinity,and chlorophyll-a concentration is relatively low,indicating that CDOM is an independent driving mechanism influenced by phytoplankton degradation,photobleaching,and water mixing.

Study on the Introduction of Plants with Colored Foliage in Zhengzhou Area and Their Adaptability

[Objective] This study aimed to preliminarily select the plants with colored foliage adaptable to Zhengzhou area. [Method] An introduction trial on 42 varieties with colored foliage was conducted, in which major ornamental characteristics, phenological phases and stress resistance of all the introduced plants with colored foliage were observed and the introduced plants were comprehensively evaluated. [Result] Golden-and Compound-leaved Maple, Red-leaved Sakura, Pink-and Compound-leaved Maple, Amur Maple, Purple-leaved Canadian Redbud with good performance were selected preliminarily, which can be applied for landscape in Zhengzhou area. Among them, Golden- and Compound-leaved Maple, Golden- and Silvery-leaved Maple and Purple-leaved Smoketree can either be planted separately, or in a line as boulevard trees, or in groups as ornamental forest; Red-leaved Sakura, Pink- and Compound-leaved Maple and Purple-leaved Canadian Redbud can be planted in groups or separately in gardens, parks or alongside streets or in front of buildings; Amur Maple with high adaptability is a excellent tree species for afforesting barren mountain. [Conclusion] This study has not only provided new excellent germplasm sources for the landscaping in Zhengzhou area, thus favoring the improvement of local landscaping level, but also provides examples and references for the introduction of plants with colored foliage in the other areas.

Development and Utilization of Naturally Colored Cocoon Resources of Bombyx mori

This paper summarized the diversity and prominent characteristics of naturally colored cocoon resources of Bombyx mori, and introduced the production methods of colored cocoons, the development and utilization of naturally colored cocoon resources at home and abroad as well as the variety breeding instances, based on which suggestions were proposed for the development of colored cocoon industry.

Breeding of a Natural Colored-Cocoon Bombyx mori Variety Shuhuang No.1

[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteristics were identified by laboratory tests and rural promotion practices in Sichuan Province.[Result]The cocoon produced by the new variety Shuhuang No.1 was golden-yellow and radiantly beautiful.Shuhuang No.1 was strong and easy to rear,uniform in development.The male and female ones could be distinguished by their larval color and markings.The cocoon yield per 10 000 the 4th instar larvae reached 20.30 kg; the length of a cocoon filament was about 1 115.0 m,and a non-broken filament was 882.95 mm long,accounting for 79.15％ of the total length.The raw silk ratio of fresh cocoon was 18.39％,and the neatness was 91.25 point.The main economic features of Shuhuang No.1 were better than those of the control.[Conclusion] The new variety had been approved by Sichuan Silkworm Evaluation Commission.It can be promoted in parent silkworm rearing areas Sichuan,Chongqing,Yangtze River basin.

Effects of Pigment Indicators on the Color Expression of Leaves of the Colored-leaf Garden Plants

[Objective] In this study, the relationship between the pigments and the color expression of leaves of colored-leaf plants was discussed. [Method] The colors of leaf blades of 6 kinds of plants were analyzed with the Royal Horticultural Soci-ety Colour Chart. The chlorophyl content, carotenoids content and anthocyanin con-tent in leaf blades were determined. In addition, the color types of leaf blades, kinds of pigments, pigment contents and pigment distributions of 6 kinds of plants were compared. [Result] The chlorophyl contents ranked as Populus canadensis Moench （green leaves） 〉 Populus deltoids cv. Zhonghuahongye （purple green leaves） 〉 Populus euramericana cv. Quanhong （red leaves）; Acer palmatum Thunb. （green leaves） 〉 Acer palmatum cv. Atropurpureum （purple red leaves） 〉 Acer pal-matum Thunb. cv. Atropurpureum （red leaves）. The ranking of anthocyanin contents was just opposite. The chlorophyl content was negatively related to the anthocyanin content. The leaf color of plants is determined by various pigments. The more the chlorophyl is, the greener the leaf is. The more the anthocyanin is, the redder the leaf is. In the colored-leaf plants, the chlorophyl content represents about 80% of the content of pigments, the carotenoids content represents about 17%, and the an-thocyanin represents about 3%. There is a difference in the chlorophyl content be-tween colored-leaf plants and green plants. However, the relatively low chlorophyl content wil not hamper the normal life activities of colored-leaf plants. [Conclusion] We hoped to provide reference and basis for the production and landscaping of col-ored-leaf plants.

Colored women in Jack London'works

This thesis studies the colored women in Jack London's works, probing into origins of his affections for and attitudes towards women on his myth-like life.

Synthesis and characterization of colored layered double hydroxides for thermal stabilizer

Colored layered double hydroxides （LDHs） can be synthesized by introducing colored cations such as Fe^3＋ and Cr^3 ＋, which call be used as thermal stabilizers for polyvinyl chloride （PVC）. The yellowish Mg/Fe and bluish Mg/Cr LDHs are prepared by the co-precipitation method. The results show that the MgsCr_ CO3 and Mg3Fe_ CO3 colored layered double hydroxides can stabilize PVC for more than 30 min under the thermal aging temperature of 180 ℃. The preparation can use cheap Mg（OH） 2 instead of MgCl2, which produces a much smaller amount of the by-product NH4Cl. It is known that NH4Cl is a cheap fertilizer that is difficult to sell; therefore, the preparation is much greener and more economic than the one using magnesium salt.

Property changes of wood-fiber/HDPE composites colored by iron oxide pigments after accelerated UV weathering

Four kinds of iron oxide pigments were added into wood-fiber/high-density-polyethylene composites （WF/HDPE） at three different concentrations, to determine the effects of pigments on the changes in the color and mechanical properties of the composites before and after UV accelerated weathering. HDPE, wood fibers, pigments and other processing additives were dry-mixed in a high-speed mixer. The mixtures were extruded by two-step extrusion process with a self-designed twin-screw/single-screw extruder system. Color of the samples was determined according to CIE 1976 L^＊a^＊b^＊ system by a spec- trophotometer and the bending properties were tested to evaluate the mechanical properties before and after accelerated UV weathering. The result shows that the modulus of elasticity of WF/HDPE did not obvi- ously changed after incorporating with the pigments, but the bending strength increased. After accelerated aging for 2000 h, both color and mechanical properties significantly changed. Iron oxide red and black performed better than the other two pigments, and the pigments dosage of 2.28% in the composites is favourable.

一种基于赋时Colored Petri网的工作流模型

工作流管理技术是 2 0世纪 90年代初新兴起的软件技术 ,其实现的关键在于工作流概念模型的建立。论文选择赋时ColoredPetri网 (TimedColoredPetriNet)作为工作流建模的主要工具 ,为了实现用Petri网来建立工作流模型 ,对 petri网做了适当的扩展以适应模型管理。最后通过 1个案例进一步阐述了基于Petri网的工作流建模技术。

Studies on Hereditary Properties of Colored Cotton in Xinjiang

[Objective] This study was to reveal the genetic characteristics of colored cotton varieties grown in Xinjiang. [Method] Five white upland and two white sea is- land cotton varieties were processed complete diallel crosses with five brown cotton varieties and five green cotton varieties respectively, constructing 96 F2 populations, and the population number of those in line with Mendel＇s law were counted. The individual fiber colors were statistically analyzed in each F2 generation. The cluster analysis was carried out to the parents based on their agronomic traits, and the SSR was used to analyze the genetic diversity of parents. [Result] Both green and brown fiber colors were determined by single nuclear gene; brown fiber color mainly presented dominant heredity, while green fiber color presented dominant, incomplete dominant or recessive heredity. The cluster analysis indicated that brown cotton vari- eties in Xinjiang had closer genetic relationships with Xinjiang-native upland cotton varieties than other upland cotton varieties in China, but distant from sea island cot- ton varieties; while green cotton varieties grown in Xinjiang showed distant genetic relationships with both native and other upland cotton varieties in China, and most distant from sea island varieties. SSR genetic diversity analysis of the parents showed that in all white/brown cross combinations, Xinluzao31 and Xincaimian11 showed the highest polymorphism while 293-ZM-2 and Xincaimian5 showed the low- est; in white/green cross combinations, Xinluzao31 and Green 85 showed the highest polymorphism while Xinluzao13 and Xingcaimian12 showed the lowest. [Conclusion] According to the genetic model and cluster analysis of colored cotton, the quality of cotton can be improved by hybridizing the colored cotton with Xinjiang native or in- land cotton varieties of China. This study provides germplasm resources and further study basis for the breeding of new colored cotton varieties, and materials to the fiber color gene mapping and cloning in future, as well as the technical assurance for the directional breeding of quality colored cotton varieties.

Total phenolic, flavonoid content, and antioxidant activity of flour, noodles, and steamed bread made from different colored wheat grains by three milling methods

The objective of this study was to evaluate the effects of wheat variety, food processing, and milling method on antioxidant properties. Black wheat variety Heibaoshi 1 had the highest total phenolic content(659.8 μg gallic acid equivalents g-1), total flavonoid content(319.3 μg rutin equivalents g-1), and antioxidant activity, whereas light purple wheat variety Shandongzimai 1 had the lowest total flavonoid content(236.2 μg rutin equivalents g-1) and antioxidant activity. Whole wheat flour and partially debranned grain flour had significantly higher total phenolic contents, total flavonoid contents, and antioxidant activity than refined flour(P < 0.05). Compared with flour, total phenolic contents, total flavonoid contents and antioxidant activity decreased in noodles and steamed bread, whereas noodles had slightly higher total phenolic and flavonoid content than steamed bread. Antioxidant activities(by ferric reducing ability of plasma assay) of steamed bread made from whole wheat flour, partially debranned grain flour, and refined flour were 23.5%, 21.1%, and 31.6% lower, respectively, than the corresponding values of flour. These results suggested that black whole wheat flour and partially debranned grain flour are beneficial to human health.

Modeling Method of Automotive Body CAN/LIN Nets Application Protocol Based on Object-oriented Colored Petri Net

Recently automotive nets are adopted to solve increasing problems in automotive electronic systems.Technologies of automotive local area network from CAN and LIN can solve the problems of the increasing of wire bunch weight and lack in module installation space.However,the multilayer automotive nets software becomes more and more complex,and the development expense is difficult to predict and to keep in check.In this paper,the modeling method of hierarchical automotive nets and the substitution operation based on object-oriented colored Petri net(OOCPN) are proposed.The OOCPN model which analyzes the software structure and validates the collision mechanism of CAN/LIN bus can speed the automobile system development.First,the subsystems are divided and modeled by object-oriented Petri net(OOPN).According to the sets of message sharing relations,the message ports among them are set and the communication gate transitions are defined.Second,the OOPN model is substituted step by step until the inner objects in the automotive body control modules(BCM) are indivisible and colored by colored Petri net(CPN).And the color subsets mark the node messages for the collision mechanism.Third,the OOCPN model of the automotive body CAN/LIN nets is assembled,which keeps the message sets and the system can be expanded.The proposed model is used to analyze features of information sharing among the objects,and it is also used to describe each subsystem real-time behavior of processing messages and implemental device controllers operating,and puts forward a reasonable software framework for the automotive body control subsystem.The research can help to design the communication model in the automotive body system effectively and provide a convenient and rapid way for developing the logical hierarchy software.

Concentration and Characterization of Colored Dissolved Organic Matter in the Surface Microlayer and Subsurface Water of the Yellow Sea and the East China Sea

The distribution and chemical properties of colored dissolved organic matter(CDOM) in the Yellow Sea and the East China Sea during December 2011-January 2012 were investigated. The input of freshwater and biological activities had an evident influence on the CDOM levels(characterized by the light absorption coefficient at the wavelength of 355 nm a_(355)) in the study area. The spatial distribution of CDOM levels displayed a gradually decreasing trend from the coastal waters(0.37 m^(-1)) to the open sea(0.18 m^(-1)). The spectral slope ratio(the slope ratio S_R defined as S_(275-295):S_(350-400)) during the cruise was correlated with salinity, and exhibited a large variation from inshore(average of 2.515) to offshore sites(average of 5.327) compared with the distribution of a_(355). The values of S_R were related to CDOM molecular weight(MW). The a_(355), S_R, and chlorophyll a in 37 samples collected from the surface microlayer were significantly correlated with those in the corresponding subsurface water samples, implying a strong exchange action between the microlayer and bulk water. The a_(355) and S_R of CDOM exhibited significant microlayer enrichment, with mean enrichment factors(EFs) of 1.72 and 1.62, respectively.

Stochastic Resonance in a Single-Mode Laser Driven by Quadratic Colored Pump Noise:Effects of Biased Amplitude Modulation Signal

A single-mode laser noise model driven by quadratic colored pump noise and biased amplitude modulationsignal is proposed.The analytic expression of signal-to-noise ratio is calculated by using a new linearized procedure.Itis found that there are three different typies of stochastic resonance in the model:the conventional form of stochasticresonance,the stochastic resonance in the broad sense,and the bona fide SR.

The satellite reversion of dissolved organic carbon (DOC) based on the analysis of the mixing behavior of DOC and colored dissolved organic matter: the East China Sea as an example

The retrieval of dissolved organic carbon （DOC） distribution by remote sensing is mainly based on the em- pirical relationship of DOC concentration and colored dissolved organic matter （CDOM） concentration in many literatures. To investigate the nature of this relationship, the distributions and mixing behaviors of DOC and CDOM are reviewed in the world＇s major estuaries and bays. It is found that, generally, the C- DOM concentration is well correlated with the salinity in most estuaries, while DOC usually shows a non- conservative behavior which leads to a weak correlation between the DOC concentration and the CDOM concentration. To establish a good satellite reversion of the DOC concentration, the East China Sea（ECS） was taken as an example, and the mixing behavior of DOC and CDOM as well as the influence of biogeo- chemical processes were analyzed except for the physical mixing process with the data from late autumn （November, 2010） and winter （December, 2009） cruises. In the two ECS cruises, the CDOM concentration was found to be tightly correlated with the salinity, influenced little by the photochemical or biological pro- cesses. The data from the winter cruise show that DOC followed a conservative mixing along the salinity gradient, while in the late autumn cruise it was significantly affected by the biological activities, resulting in a poor correlation between the DOC and the CDOM. Accordingly, an improved DOC algorithm （CSDM） was proposed： when the biological influence was significant （Chl a greater than 0.8 μg/dm3）, DOC was retrieved by the conservative and biological model, and if the conservative mixing was dominant （Chl a less than 0.8 μg/dm3）, the direct DOC concentration and CDOM concentration relationship was used. Based on the pro- posed algorithm, a reasonable DOC distribution for the ECS from satellite was obtained in this study, and the proposed method can be applied to the other large river-dominant marginal sea.