[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Co...[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Cordyceps Sinensis effluent and betaine. [Result] Different compositions in Cordyceps Sinensis/etaine compound feed nutriment would be well separated by the method. [Conclusion] The method provides a suitable platform of separation and analysis for Cordyceps Sinensis /Betaine compound feed nutriment.展开更多
[Objective] The research aimed to explore the effects of comminution technology on the dissolution of active components from compound feed additive of Strobi/anthes cusia. [Method] Using active component adenosine in ...[Objective] The research aimed to explore the effects of comminution technology on the dissolution of active components from compound feed additive of Strobi/anthes cusia. [Method] Using active component adenosine in principal herb S. cusia and chlorogenic acid in minister drug Lonicera japonica Thunb. as dissolution indices,the effects of ultrafine comminution on the dissolution degree of compound feed additive of S. cusia were analyzed. [ Result] The dissolution degree of adenosine and chlorogenic acid in ultra micro particles were obviously higher than that of common particles. Ultrafine comminution could significantly quicken the dissolution rate of adenosine and chlorogenic acid in compound feed additive of S. cusia, shorten the extraction time, avoid the breakage of active components in long-time extraction process,and meanwhile saved the energy greatly. [ Conclusion] The ultrafine comminution technology could obviously increase the dissolution degree of active components in compound feed additive of S. cusia.展开更多
[目的]建立同时检测配合饲料中64种药物的超高效液相色谱-三重四极杆串联质谱(ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry,UHPLC-MS/MS)法,提高非法添加物的检测效率。[方法]采用Waters...[目的]建立同时检测配合饲料中64种药物的超高效液相色谱-三重四极杆串联质谱(ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry,UHPLC-MS/MS)法,提高非法添加物的检测效率。[方法]采用Waters HSS T3型色谱柱(2.1 mm×100 mm,1.8μm)进行分离,流动相A为0.1%甲酸水溶液,流动相B为含0.1%甲酸的乙腈溶液,梯度洗脱,流速为0.40 mL/min,进样量为2μL;采用电喷雾离子源正离子扫描模式进行检测,多反应监测模式进行信号采集。比较4种样品提取溶剂以及2种固相萃取柱处理对目标药物的回收率,确定样品前处理的最佳方法。利用建立的UHPLC-MS/MS法对宁夏回族自治区不同来源的100批次配合饲料样品进行64种药物检测。[结果]配合饲料样品均质后,用含0.2%甲酸的乙腈水溶液(乙腈∶水=8∶2,V/V)提取,利用Oasis PRiME HLB型固相萃取柱对样品净化,多数目标药物的回收率在60%以上。64种药物在浓度为5.0~200.0μg/L的范围内线性关系良好,相关系数(R)均大于0.99;不同药物的定量限在5.0~10.0μg/kg;阳性添加5.0、20.0、50.0μg/kg 3个浓度的平均回收率在41.00%~120.49%,批内相对标准偏差(RSD)在0.54%~15.94%,批间RSD在1.25%~13.64%。在100个批次的配合饲料样品中均未检出目标药物。[结论]建立的UHPLC-MS/MS法线性关系良好、回收率高、精密度好,具有较高的重现性和较好的可操作性,可用于配合饲料中非法添加64种药物的筛查。展开更多
为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squar...为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)方法筛选与不同养殖方式相关的差异性风味物质。结果表明:平养组和笼养组共有的挥发性风味物质27种,主要为酚类、醇类和烃类。挥发性风味物质中,己醛、1-辛烯-3-醇、E-2-壬烯醛、正己醇、壬醛、2,3-戊二酮、癸醛、2,3-辛二酮、E-2-辛烯醛为具有显著性差异的挥发性风味物质。综上,这一研究可为地方鸡肉品质基于风味物质的评价提供科学依据。展开更多
In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk, with corn, wheat bran and corn stalk as the raw materials, 13 different compound feed were design...In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk, with corn, wheat bran and corn stalk as the raw materials, 13 different compound feed were designed by Minitab （R） 15.1.0.0. The design was then divided into two groups based on addition or without addition of vegetable leaves to study the effects of compound feed and vegetable leaves on growth and de- velopment of T. molitor. The results showed that body weight, duration, crude protein content and pupation rate of larvae and emergence rate of adults were significantly affected by compound feed and vegetable leaves （ P 〈 0.05）. The larvae bred with feed 5 had the largest body weight, with the shortest duration; the larvae bred with feed 3 had the highest pupation rate; the larvae bred with feed 7 had the highest crude protein content; the adult bred with feed 9 had the largest emergence rate. Overall breeding effect of feed 7 ＋ vegetable leaves was better than any other groups, with ideal indicators, higher utilization rate and lower breeding cost. Its formula was w （wheat bran） = 33.33%, w （corn） = 33.33%, w （corn stalks） = 33.33%. Therefore, the growth of T. molitorwas significantly affected by feed types, and feed 7 ＋ vegetable leaves could be promoted in the production.展开更多
文摘[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Cordyceps Sinensis effluent and betaine. [Result] Different compositions in Cordyceps Sinensis/etaine compound feed nutriment would be well separated by the method. [Conclusion] The method provides a suitable platform of separation and analysis for Cordyceps Sinensis /Betaine compound feed nutriment.
基金funded by Guangdong Science and Technology Study Program(2010B090400529)
文摘[Objective] The research aimed to explore the effects of comminution technology on the dissolution of active components from compound feed additive of Strobi/anthes cusia. [Method] Using active component adenosine in principal herb S. cusia and chlorogenic acid in minister drug Lonicera japonica Thunb. as dissolution indices,the effects of ultrafine comminution on the dissolution degree of compound feed additive of S. cusia were analyzed. [ Result] The dissolution degree of adenosine and chlorogenic acid in ultra micro particles were obviously higher than that of common particles. Ultrafine comminution could significantly quicken the dissolution rate of adenosine and chlorogenic acid in compound feed additive of S. cusia, shorten the extraction time, avoid the breakage of active components in long-time extraction process,and meanwhile saved the energy greatly. [ Conclusion] The ultrafine comminution technology could obviously increase the dissolution degree of active components in compound feed additive of S. cusia.
文摘为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)方法筛选与不同养殖方式相关的差异性风味物质。结果表明:平养组和笼养组共有的挥发性风味物质27种,主要为酚类、醇类和烃类。挥发性风味物质中,己醛、1-辛烯-3-醇、E-2-壬烯醛、正己醇、壬醛、2,3-戊二酮、癸醛、2,3-辛二酮、E-2-辛烯醛为具有显著性差异的挥发性风味物质。综上,这一研究可为地方鸡肉品质基于风味物质的评价提供科学依据。
基金Supported by Agricultural Research Project of Shaanxi Provincial Science and Technology Department(2012.k02-10)Innovation and Entrepreneurship Training Project of University Students in Shaanxi Province(1252)Special Funds of Ecology for Construction of High-level University in Shaanxi Province(2012SXTS03)
文摘In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk, with corn, wheat bran and corn stalk as the raw materials, 13 different compound feed were designed by Minitab （R） 15.1.0.0. The design was then divided into two groups based on addition or without addition of vegetable leaves to study the effects of compound feed and vegetable leaves on growth and de- velopment of T. molitor. The results showed that body weight, duration, crude protein content and pupation rate of larvae and emergence rate of adults were significantly affected by compound feed and vegetable leaves （ P 〈 0.05）. The larvae bred with feed 5 had the largest body weight, with the shortest duration; the larvae bred with feed 3 had the highest pupation rate; the larvae bred with feed 7 had the highest crude protein content; the adult bred with feed 9 had the largest emergence rate. Overall breeding effect of feed 7 ＋ vegetable leaves was better than any other groups, with ideal indicators, higher utilization rate and lower breeding cost. Its formula was w （wheat bran） = 33.33%, w （corn） = 33.33%, w （corn stalks） = 33.33%. Therefore, the growth of T. molitorwas significantly affected by feed types, and feed 7 ＋ vegetable leaves could be promoted in the production.
