[Objective]The aim was to prove that the mitochondrial genes of Cyt b and 12S rRNA with different evolutional rates have effects on the topological structures of phylogenetic trees.[Method]The complete sequences of Cy...[Objective]The aim was to prove that the mitochondrial genes of Cyt b and 12S rRNA with different evolutional rates have effects on the topological structures of phylogenetic trees.[Method]The complete sequences of Cyt b and 12S rRNA from 15 species in 12 families of snakes were downloaded and extracted from GenBank,while their molecular phylogenetic trees were constructed by Maximum Likelihood（ML） method with GTR ＋I ＋G substitute model based on PAUP4.0 software.[Result]With the same software,methods and species,the difference in topological structures of phylogenetic trees was mainly due to different evolutional rates of Cyt b and 12S rRNA genes.[Conclusion]In studies on phylogenetic trees,aimed to different research species and purposes,phylogenetic trees should be constructed by choosing the correct and appropriate genes.展开更多
[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mit...[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.[Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.[Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab.展开更多
为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大...为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。展开更多
基金Supported by Natural Science Fund in Guangdong(915102600100-0003)Project for Excellent Young Scientists and Engineers in Guangdong Academy of Sciences(200804)Fund of Open Laboratory for Protection and Utilization of Wildlife in Guangdong(200901)~~
文摘[Objective]The aim was to prove that the mitochondrial genes of Cyt b and 12S rRNA with different evolutional rates have effects on the topological structures of phylogenetic trees.[Method]The complete sequences of Cyt b and 12S rRNA from 15 species in 12 families of snakes were downloaded and extracted from GenBank,while their molecular phylogenetic trees were constructed by Maximum Likelihood（ML） method with GTR ＋I ＋G substitute model based on PAUP4.0 software.[Result]With the same software,methods and species,the difference in topological structures of phylogenetic trees was mainly due to different evolutional rates of Cyt b and 12S rRNA genes.[Conclusion]In studies on phylogenetic trees,aimed to different research species and purposes,phylogenetic trees should be constructed by choosing the correct and appropriate genes.
基金Supported by Application Basic Research Foundation of TianjinInternational Cooperation Fund(033803511,033803511G)the Tianjin Higher Education Science and Technology Development Fund(2004BA31)~~
文摘[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.[Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.[Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab.
文摘为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。
