The chirp sub-bottom profiler,for its high resolution,easy accessibility and cost-effectiveness,has been widely used in acoustic detection.In this paper,the acoustic impedance and grain size compositions were obtained...The chirp sub-bottom profiler,for its high resolution,easy accessibility and cost-effectiveness,has been widely used in acoustic detection.In this paper,the acoustic impedance and grain size compositions were obtained based on the chirp sub-bottom profiler data collected in the Chukchi Plateau area during the 11th Arctic Expedition of China.The time-domain adaptive search matching algorithm was used and validated on our established theoretical model.The misfit between the inversion result and the theoretical model is less than 0.067%.The grain size was calculated according to the empirical relationship between the acoustic impedance and the grain size of the sediment.The average acoustic impedance of sub-seafloor strata is 2.5026×10^(6) kg(s m^(2))^(-1)and the average grain size(θvalue)of the seafloor surface sediment is 7.1498,indicating the predominant occurrence of very fine silt sediment in the study area.Comparison of the inversion results and the laboratory measurements of nearby borehole samples shows that they are in general agreement.展开更多
Based on the fifth-order Stokes regular wave theory, a simplified model for extreme-wave kinematics in deep sea was developed. In this model, from the wave records the average of two neighboring wave periods for the e...Based on the fifth-order Stokes regular wave theory, a simplified model for extreme-wave kinematics in deep sea was developed. In this model, from the wave records the average of two neighboring wave periods for the extreme crest or trough was defined as the period of the Stokes wave by the up and down zero-crossing methods. Then the input wave amplitude was deduced by substituting the wave period and extreme crest or trough into the expression for the fifth-order Stokes wave elevation. Thus the corresponding formula for the wave velocity can be used to describe kinematics beneath the extreme wave. By comparison with the published numerical models and experimental data, the proposed model is validated to be able to calculate the extreme wave velocity rather easily and accurately.展开更多
There is a great demand for in-situ real-time chemical sensors in the oceanographic research, to measure the chemical components under the deep sea. The ISE (Ion Selective Electrode) is commonly used as a detecting pa...There is a great demand for in-situ real-time chemical sensors in the oceanographic research, to measure the chemical components under the deep sea. The ISE (Ion Selective Electrode) is commonly used as a detecting part of deep-sea electro-chemical sensors. The paper highlights the solidification and micromation of the working and reference electrodes. The sensors of pH and H 2S with a thermal probe are accomplished after the solution of configuration of electrodes and signal processing. The sensor system has been tested successfully in the cruise of DY105-12, 14 sponsored by China Ocean Mineral Research and Exploitation Association(COMRA).展开更多
Based on more than 4000 km 2D seismic data and seismic stratigraphic analysis, we discussed the extent and formation mechanism of the Qiongdongnan deep sea channel. The Qiongdongnan deep sea channel is a large incised...Based on more than 4000 km 2D seismic data and seismic stratigraphic analysis, we discussed the extent and formation mechanism of the Qiongdongnan deep sea channel. The Qiongdongnan deep sea channel is a large incised channel which extends from the east boundary of the Yinggehai Basin, through the whole Qiongdongnan and the Xisha trough, and terminates in the western part of the northwest subbasin of South China Sea. It is more than 570 km long and 4–8 km wide. The chaotic (or continuous) middle (or high) amplitude, middle (or high) continuity seismic facies of the channel reflect the different lithological distribution of the channel. The channel formed as a complex result of global sea level drop during early Pliocene, large scale of sediment supply to the Yinggehai Basin, inversion event of the Red River strike-slip fault, and tilted direction of the Qiongdongnan Basin. The large scale of sediment supply from Red River caused the shelf break of the Yinggehai Basin to move torwards the S and SE direction and developed large scale of prograding wedge from the Miocene, and the inversion of the Red River strike-slip fault induced the sediment slump which formed the Qiongdongnan deep sea channel.展开更多
A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC...A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.展开更多
A flexible connector of lifting pipes in a deep sea mining system is designed. The buttress thread ( based on API standard) is used and foreign experience in connector design is referred to. With this kind of connec...A flexible connector of lifting pipes in a deep sea mining system is designed. The buttress thread ( based on API standard) is used and foreign experience in connector design is referred to. With this kind of connector, the lifting pipe wiU only bear the axial force, free of moment. The strength of the connector is analyzed in detail, including the connecting strength of the buttress thread, the dynamic load of the pipe system, pressures inside and outside of the pipe, the lateral stress of the pipe wall and so on. Especially, a geometric model is built for 3-D contact stress analysis. The distribution graph of contact stress is presented. It is indicated that the strength of the spherical connector meets the demand.展开更多
In order to study buckling propagation mechanism in deep sea pipelines, the contact between pipeline's inner walls in buckling process was studied. A two-dimensional ring model was used to represent the pipeline a...In order to study buckling propagation mechanism in deep sea pipelines, the contact between pipeline's inner walls in buckling process was studied. A two-dimensional ring model was used to represent the pipeline and a nonlinear spring model was adopted to simulate the contact between inner walls. Based on the elastoplastic constitutive relationship and the principle of virtual work theory, the coupling effect of pipeline's nonlinear large deformation and wall contact was included in the theoretical analysis with the aid of MATLAB, and the application scope of the theoretical model was also discussed. The calculated results show that during the loading process, the change in external pressure is closely related to the distribution of section stress, and once the walls are contacting each other, the external pressure increases and then remains stable after it reaches a specific value. Without fracture, the pipeline section will stop showing deformation. The results of theoretical calculations agree well with those of numerical simulations. Finally, in order to ensure reliability and accuracy of the theoretical results, the collapse pressure and propagation pressure were both verified by numerical simulations and experiments. Therefore, the theoretical model can be used to analyze pipeline's buckling deformation and contact between pipeline's inner walls, which forms the basis for further research on three-dimensional buckling propagation.展开更多
Navigation is a critical requirement for the operation of Autonomous Underwater Vehicles(AUVs).To estimate the vehicle position,we present an algorithm using an extended Kalman filter(EKF) to integrate dead-reckon...Navigation is a critical requirement for the operation of Autonomous Underwater Vehicles(AUVs).To estimate the vehicle position,we present an algorithm using an extended Kalman filter(EKF) to integrate dead-reckoning position with acoustic ranges from multiple beacons pre-deployed in the operating environment.Owing to high latency,variable sound speed multipath transmissions and unreliability in acoustic measurements,outlier recognition techniques are proposed as well.The navigation algorithm has been tested by the recorded data of deep sea AUV during field operations in a variety of environments.Our results show the improved performance over prior techniques based on position computation.展开更多
In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alk...In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alkaloids, diketopiperazine V(1b), brevianamide Q(2), brevianamide R(3), brevianamide K(4), and brevianamide E(5), were isolated from the Et OAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques(NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0%(1a and 1b), 53.7%(2), 46.2%(3), 61.4%(4) and 19.3%(5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.展开更多
This paper describes the implementation of a data logger for the real-time in-situ monitoring of hydrothermal systems. A compact mechanical structure ensures the security and reliability of data logger when used under...This paper describes the implementation of a data logger for the real-time in-situ monitoring of hydrothermal systems. A compact mechanical structure ensures the security and reliability of data logger when used under deep sea. The data logger is a battery powered instrument, which can connect chemical sensors (pH electrode, H2S electrode, H2 electrode) and temperature sensors. In order to achieve major energy savings, dynamic power management is implemented in hardware design and software design. The working current of the data logger in idle mode and active mode is 15 μA and 1.44 mA respectively, which greatly extends the working time of battery. The data logger has been successftdly tested in the first Sino-American Cooperative Deep Submergence Project from August 13 to September 3, 2005.展开更多
The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experime...The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experiment of spherical joint connecting deep sea mining system has been done in band six marine conditions. The results indicate that the moment of spherical joint connecting deep sea mining is smaller than that of the thread connected ones, the lifting pipe of sphelical joint is "flexible pipe". The flexural torque of the articulated lifting pipe system in pump and buffer is generally periodic variation with some irregularity, the value is stable on 60 N·S, and it is obviously smaller than that of the fixed lifting pipe system; The total displacement exhibits cyclic variation pattern, and the periodicity of them is longer than that of sea current. The results of experiment and simulation are basically consistent. And the analysis in the paper offers theoretical foundation of 1000 m deep sea mining system in China.展开更多
Depth-dependent distribution patterns of bacterial and archaeal communities in deep sea water column around the Ninetyeast Ridge in the Indian Ocean were investigated using 16S rRNA gene profiling.Sampling was conduct...Depth-dependent distribution patterns of bacterial and archaeal communities in deep sea water column around the Ninetyeast Ridge in the Indian Ocean were investigated using 16S rRNA gene profiling.Sampling was conducted at the northern Ninetyeast Ridge(1°59.89′N–9°59.70′S,87°58.90′E–88°00.03′E)from September to November 2016 where samples were collected from the bathyal(1000 m)to bathypelagic depths(>4000 m)in four different stations.A total of 1565405 clean data falling into 6712 bacterial OTUs and 1452727 clean data falling into 806 archaeal OTUs based on 97%similarity level were analyzed.Most of the bacterial 16S rRNA gene sequences were affiliated with Gammaproteobacteria,followed by Alphaproteobacteria and Bacteroidia.The archaeal 16S rRNA gene sequences mostly affiliated to Nitrososphaeria(Thaumarchaeota)dominated with relative abundances ranging from 52.68%to 97.2%,followed by Thermoplasmata(Euryarchaeota).Vertical partitioning of bacterial and archaeal communities among different water layers was observed.Canonical correspondence analysis(CCA)and Spearman’s correlations revealed that depth(P=0.003),dissolved oxygen(P=0.019),and nitrite(P=0.033)were the main environmental factors affecting bacterial community structure at genus level in the Ninetyeast Ridge.On the other hand,the first two CCA axes accounted for 74.4%of the explained total variance,it seems that the archaeal communities at genus level were heavily influenced by the environmental variables including depth,dissolved oxygen(DO),nitrite,salinity,phosphate,ammonia,nitrate,and silicate,but none of them exhibited any significant correlation on the structuring(P>0.1).展开更多
As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant an...As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.展开更多
The present status of deep sea bed mining technology for polymetallic nodules has been critically examined in light of its history of development the UNCLoS (the United Nation Convention on the Law of the Sea) regime ...The present status of deep sea bed mining technology for polymetallic nodules has been critically examined in light of its history of development the UNCLoS (the United Nation Convention on the Law of the Sea) regime and the current trends in research.The critical technologies have been highlighted and the need for system integration underscored. The deep sea bed environment and the engineering challenge were presented and the necessary features of the deep sea bed mining system for commercial mining was analyzed. The key accomplishments, problems and future bends in research in the development of the mining system are also discussed. Finally, it points out that the technology gaps can be filled by the bine commercial mining is undertaken as a result of market conditions in its favor.展开更多
The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the ...The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the 'Beyond Plate Tectonics Theory' where a combination of conceptual models and numerical modeling on plume tectonics and plate tectonics has enabled new insights into the structural and tectonic architecture and processes in the deep interior and deep sea. This paper synthesizes developments of structural geology and tectonics from a macroscopic perspective in deep interior and deep sea. Four key techniques are also reviewed: satellite altimetry for surface structures in deep-sea multi-beam sea-floor mapping; tomography for tectonics of the deep interior; diverse modeling approaches and software for unfolding dynamic evolution; and techniques for HT/HP experiments on material rheology and in situ component measurements.展开更多
The National Oceanic and Atmospheric Administration reports a 95% decline in the oldest Arctic ice over the last 33 years [1], while the National Aeronautics and Space Administration states that summer Arctic Sea Ice ...The National Oceanic and Atmospheric Administration reports a 95% decline in the oldest Arctic ice over the last 33 years [1], while the National Aeronautics and Space Administration states that summer Arctic Sea Ice Extent (SIE) is shrinking by 12.2% per decade since 1979 due to warmer temperatures [2]. Given the rapidly changing Arctic conditions, accurate prediction models are crucial. Deep learning models developed for Arctic forecasts primarily focus on exploring convolutional neural networks (CNN) and convolutional Long Short-Term Memory (LSTM) networks, while the exploration of the power of LSTM networks is limited. In this research, we focus on enhancing the performance of an LSTM network for predicting monthly Arctic SIE. We leverage five climate and atmospheric variables, validated for their correlation with SIE in prior studies [3]. We utilize the Spearman’s rank correlation and ExtraTrees regressor to enhance our understanding of the importance of the five variables in predicting SIE. We further enhance our predictor variables with seasonal information, lagged time steps, and a linear regression simulated SIE that accounts for the influence of past SIE on current SIE. Statistical methods guide our selection of data scalers and best evaluation metrics for our model. By experimenting with hyperparameter optimization and advanced deep learning training techniques, such as batch sizes, number of neurons, early stopping, and model checkpoint, our model achieved a Mean Absolute Error (MAE) of 0.191 and R2 of 0.996, underscoring its ability to account for nearly all the variance in our data and holds great promise for the prediction of SIE.展开更多
Solemyidae is an ancient group of protobranch bivalves,and most solemyids are symbiotic with chemoautotrophic and gill-hosted bacteria,enabling them to survive in unusual habitats such as deepsea chemosynthetic enviro...Solemyidae is an ancient group of protobranch bivalves,and most solemyids are symbiotic with chemoautotrophic and gill-hosted bacteria,enabling them to survive in unusual habitats such as deepsea chemosynthetic environments.However,evolution of the mitogenomes in this family and their phylogenetic relationships remain poorly understood.The complete mitogenome of Acharax sp.was determined and compared with other available mitogenomes of solemyids.The mitogenome of Acharax sp.is 18970 bp in length and consists of 13 protein-coding genes,2 ribosomal RNA genes,and 22 transfer RNA genes.The gene arrangement was identical to those of other sequenced solemyids.For the present five mitogenomes of Solemyidae species,all protein-coding genes were initiated with the typical ATD(ATA,ATG,and ATT)codon and terminated with the TAA/TAG codon.Furthermore,the incomplete termination codon was detected.The Ka/Ks ratio analyses indicated that 13 protein-coding genes of five Solemyidae mitogenomes suffered strong purifying selection.Compared to 45 existing shallow water equivalents,the 18 available mitogenomes from the deep-sea,including the Acharax sp.in this study,show significantly more nonpolar amino acids in the 13 protein-coding genes,which indicates the adaptation to the deep-sea environment.The phylogenetic tree based on 48 Bivalvia complete mitogenomes provided further information to support the scientific classification of protobranchs.The relationships among Solemyidae were assessed based on 2 mitochondrial(16S rRNA and COX1)and 3 nuclear(18S rRNA,28S rRNA,and histone H3)gene sequences from 17 in-group species.The two genera Acharax and Solemya formed a monophyletic clade each,and Acharax sp.clustered with previously reported Acharax bivalves with high support values.展开更多
Drilling wells reveal that the organic matter abundance of Miocene marine source rocks in shallow water area of the Qiongdongnan Basin is relatively low with poor hydrocarbon generation poten- tial. However, in some d...Drilling wells reveal that the organic matter abundance of Miocene marine source rocks in shallow water area of the Qiongdongnan Basin is relatively low with poor hydrocarbon generation poten- tial. However, in some drilling wells of deep water area close to the central depression belt, Miocene marine source rocks with better organic matter abundance and hydrocarbon generation have been found, which have achieved better source rock standard based on the analysis of geochemical charac- teristics. Although there are no exploratory wells in deep water area of the research region, through the comparative analysis of geochemical data of several typical exploratory wells respectively from shallow water area in the basin, central depression belt margin in deep-water area of the basin and Site 1148 of deep sea drilling in the South China Sea Basin, it reveals that the tendency of the quality of source rocks becomes positive gradually from delta to bathyal environment, which then becomes negative as in deep oceanic environment. Owing to the lack of terrestrial organic matter input, the important controlling factors of Miocene marine source rocks in the Qiongdongnan Basin are ocean productivity and preservation conditions of organic matter. The element geochemistry data indicate that the tendency of the paleoproductivity and the preservation conditions of organic matter become positive as water depth increase from shallow area to bathyal area close to central depression belt. So it is speculated that there must exist high quality source rocks in the central depression area where the preservation conditions of organic matter are much better. Besides, in theory, in oxygen-poor zone of oceanic environment at the water depth 400–1 000 m, the preservation conditions of organic matter are well thus forming high-quality marine source rocks. The result- ing speculation, it is reasonable to consider that there are high hydrocarbon generation potential source rocks in bathyal environment of the Qiongdongnan Basin, especially at the water depth 400– 1 000 m.展开更多
A metal-dissolved oxygen seawater battery(SWB)uses metal and dissolved oxygen as the reactants,and it is ideal for use as a long-time low-power distributed power supply in deep sea,due to its advantages of open struct...A metal-dissolved oxygen seawater battery(SWB)uses metal and dissolved oxygen as the reactants,and it is ideal for use as a long-time low-power distributed power supply in deep sea,due to its advantages of open structure in service without electrolyte.However,several simulating deep-sea environmental factors,such as flow rate,dissolved oxygen concentration,and temperature of seawater may af fect the oxygen reduction reaction(ORR)rate and the stability of electrochemically modified polyacrylonitrile-based carbon fiber brush(MPAN-CFB)cathode,which was studied by steady-state polarization and galvanostatic discharge methods.In addition,the scales formed on MPAN-CFB surface were characterized by SEM and XRD.Results show that the ORR rate increased quickly with the increase of the seawater flow rate up to 3 cm/s,and then gradually stabilized.Moreover,the ORR rate was largely af fected by dissolved oxygen concentration,and the concentration of>3 mg/L was favorable.Compared with surface layer temperature of 15℃,the low temperature of deep sea(4℃)has a negligible ef fect on ORR rate.When the working current is too high,it will lead to the formation of CaCO_3 scales(aragonite)of at the cathodic surface,resulting in the decrease of ORR rate,and consequently the damage to the long-time stability of MPAN-CFB.展开更多
基金supported by the National Key R&D Program of China (No.2021YFC2801202)the National Natural Science Foundation of China (No.42076224)the Fundamental Research Funds for the Central Universities (No.202262012)。
文摘The chirp sub-bottom profiler,for its high resolution,easy accessibility and cost-effectiveness,has been widely used in acoustic detection.In this paper,the acoustic impedance and grain size compositions were obtained based on the chirp sub-bottom profiler data collected in the Chukchi Plateau area during the 11th Arctic Expedition of China.The time-domain adaptive search matching algorithm was used and validated on our established theoretical model.The misfit between the inversion result and the theoretical model is less than 0.067%.The grain size was calculated according to the empirical relationship between the acoustic impedance and the grain size of the sediment.The average acoustic impedance of sub-seafloor strata is 2.5026×10^(6) kg(s m^(2))^(-1)and the average grain size(θvalue)of the seafloor surface sediment is 7.1498,indicating the predominant occurrence of very fine silt sediment in the study area.Comparison of the inversion results and the laboratory measurements of nearby borehole samples shows that they are in general agreement.
基金Supported by the NSFC (under Grant Nos.5070900 and 10772040)the National High Tech Research and Development Program of China (2006AA09A109-3)
文摘Based on the fifth-order Stokes regular wave theory, a simplified model for extreme-wave kinematics in deep sea was developed. In this model, from the wave records the average of two neighboring wave periods for the extreme crest or trough was defined as the period of the Stokes wave by the up and down zero-crossing methods. Then the input wave amplitude was deduced by substituting the wave period and extreme crest or trough into the expression for the fifth-order Stokes wave elevation. Thus the corresponding formula for the wave velocity can be used to describe kinematics beneath the extreme wave. By comparison with the published numerical models and experimental data, the proposed model is validated to be able to calculate the extreme wave velocity rather easily and accurately.
基金The research program was financially supported by the Joint Program of Chinese 863 Project (Grant No. 2001AA612020 4) and the sea trial support from COMRA, China Ocean Mineral Research and Exploitation Association as well.
文摘There is a great demand for in-situ real-time chemical sensors in the oceanographic research, to measure the chemical components under the deep sea. The ISE (Ion Selective Electrode) is commonly used as a detecting part of deep-sea electro-chemical sensors. The paper highlights the solidification and micromation of the working and reference electrodes. The sensors of pH and H 2S with a thermal probe are accomplished after the solution of configuration of electrodes and signal processing. The sensor system has been tested successfully in the cruise of DY105-12, 14 sponsored by China Ocean Mineral Research and Exploitation Association(COMRA).
基金Supported by the National High Technology Research and Development Program of China (863 Program, 2006AA09Z349)the National Basic Research Program of China (2007CB411703)
文摘Based on more than 4000 km 2D seismic data and seismic stratigraphic analysis, we discussed the extent and formation mechanism of the Qiongdongnan deep sea channel. The Qiongdongnan deep sea channel is a large incised channel which extends from the east boundary of the Yinggehai Basin, through the whole Qiongdongnan and the Xisha trough, and terminates in the western part of the northwest subbasin of South China Sea. It is more than 570 km long and 4–8 km wide. The chaotic (or continuous) middle (or high) amplitude, middle (or high) continuity seismic facies of the channel reflect the different lithological distribution of the channel. The channel formed as a complex result of global sea level drop during early Pliocene, large scale of sediment supply to the Yinggehai Basin, inversion event of the Red River strike-slip fault, and tilted direction of the Qiongdongnan Basin. The large scale of sediment supply from Red River caused the shelf break of the Yinggehai Basin to move torwards the S and SE direction and developed large scale of prograding wedge from the Miocene, and the inversion of the Red River strike-slip fault induced the sediment slump which formed the Qiongdongnan deep sea channel.
文摘A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.
基金This research was financially supported by China Ocean Mineral Resources R &D Association (Grant No. DY105-03-02-17) .
文摘A flexible connector of lifting pipes in a deep sea mining system is designed. The buttress thread ( based on API standard) is used and foreign experience in connector design is referred to. With this kind of connector, the lifting pipe wiU only bear the axial force, free of moment. The strength of the connector is analyzed in detail, including the connecting strength of the buttress thread, the dynamic load of the pipe system, pressures inside and outside of the pipe, the lateral stress of the pipe wall and so on. Especially, a geometric model is built for 3-D contact stress analysis. The distribution graph of contact stress is presented. It is indicated that the strength of the spherical connector meets the demand.
基金supported by the National Natural Science Foundation of China (Grant Nos. 51239008 and 51179126)the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011ZX05026-005)
文摘In order to study buckling propagation mechanism in deep sea pipelines, the contact between pipeline's inner walls in buckling process was studied. A two-dimensional ring model was used to represent the pipeline and a nonlinear spring model was adopted to simulate the contact between inner walls. Based on the elastoplastic constitutive relationship and the principle of virtual work theory, the coupling effect of pipeline's nonlinear large deformation and wall contact was included in the theoretical analysis with the aid of MATLAB, and the application scope of the theoretical model was also discussed. The calculated results show that during the loading process, the change in external pressure is closely related to the distribution of section stress, and once the walls are contacting each other, the external pressure increases and then remains stable after it reaches a specific value. Without fracture, the pipeline section will stop showing deformation. The results of theoretical calculations agree well with those of numerical simulations. Finally, in order to ensure reliability and accuracy of the theoretical results, the collapse pressure and propagation pressure were both verified by numerical simulations and experiments. Therefore, the theoretical model can be used to analyze pipeline's buckling deformation and contact between pipeline's inner walls, which forms the basis for further research on three-dimensional buckling propagation.
基金financially supported by the National Natural Science Foundation of China(Grant No.51309215)
文摘Navigation is a critical requirement for the operation of Autonomous Underwater Vehicles(AUVs).To estimate the vehicle position,we present an algorithm using an extended Kalman filter(EKF) to integrate dead-reckoning position with acoustic ranges from multiple beacons pre-deployed in the operating environment.Owing to high latency,variable sound speed multipath transmissions and unreliability in acoustic measurements,outlier recognition techniques are proposed as well.The navigation algorithm has been tested by the recorded data of deep sea AUV during field operations in a variety of environments.Our results show the improved performance over prior techniques based on position computation.
基金supported by Chinese National Science Fund (No. 41176120)Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. BS2010HZ027)+1 种基金Specialized Research Fund for the Doctoral Program of Higher Education (No. 20100132120026)the Fifty First Postdoctoral Fund of China (2012M511552)
文摘In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alkaloids, diketopiperazine V(1b), brevianamide Q(2), brevianamide R(3), brevianamide K(4), and brevianamide E(5), were isolated from the Et OAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques(NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0%(1a and 1b), 53.7%(2), 46.2%(3), 61.4%(4) and 19.3%(5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.
基金supported by the International Cooperative Key Project(Grant No.2004DFA04900)Ministry of Sciences and Technology of PRC,and the National Natural Science Foundation of China (Grant Nos.40637037 and 50675198)
文摘This paper describes the implementation of a data logger for the real-time in-situ monitoring of hydrothermal systems. A compact mechanical structure ensures the security and reliability of data logger when used under deep sea. The data logger is a battery powered instrument, which can connect chemical sensors (pH electrode, H2S electrode, H2 electrode) and temperature sensors. In order to achieve major energy savings, dynamic power management is implemented in hardware design and software design. The working current of the data logger in idle mode and active mode is 15 μA and 1.44 mA respectively, which greatly extends the working time of battery. The data logger has been successftdly tested in the first Sino-American Cooperative Deep Submergence Project from August 13 to September 3, 2005.
基金This research was financially supported by China Ocean Mineral Resources Reasearch and Design Association (GrantNo DY105-03-02-17)Ph.D Programs Foundation of Ministry of Education of China (Grant No20060008025)
文摘The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experiment of spherical joint connecting deep sea mining system has been done in band six marine conditions. The results indicate that the moment of spherical joint connecting deep sea mining is smaller than that of the thread connected ones, the lifting pipe of sphelical joint is "flexible pipe". The flexural torque of the articulated lifting pipe system in pump and buffer is generally periodic variation with some irregularity, the value is stable on 60 N·S, and it is obviously smaller than that of the fixed lifting pipe system; The total displacement exhibits cyclic variation pattern, and the periodicity of them is longer than that of sea current. The results of experiment and simulation are basically consistent. And the analysis in the paper offers theoretical foundation of 1000 m deep sea mining system in China.
基金Supported by the China Ocean Mineral Resources Research and Development Association Program(Nos.DY135-E2-1-01,DY135-E2-4-00)the China Global Sea-Atmosphere Interaction Research Program(No.GASI-02-IND-STSsum)the S&T Innovation Project of Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ14)。
文摘Depth-dependent distribution patterns of bacterial and archaeal communities in deep sea water column around the Ninetyeast Ridge in the Indian Ocean were investigated using 16S rRNA gene profiling.Sampling was conducted at the northern Ninetyeast Ridge(1°59.89′N–9°59.70′S,87°58.90′E–88°00.03′E)from September to November 2016 where samples were collected from the bathyal(1000 m)to bathypelagic depths(>4000 m)in four different stations.A total of 1565405 clean data falling into 6712 bacterial OTUs and 1452727 clean data falling into 806 archaeal OTUs based on 97%similarity level were analyzed.Most of the bacterial 16S rRNA gene sequences were affiliated with Gammaproteobacteria,followed by Alphaproteobacteria and Bacteroidia.The archaeal 16S rRNA gene sequences mostly affiliated to Nitrososphaeria(Thaumarchaeota)dominated with relative abundances ranging from 52.68%to 97.2%,followed by Thermoplasmata(Euryarchaeota).Vertical partitioning of bacterial and archaeal communities among different water layers was observed.Canonical correspondence analysis(CCA)and Spearman’s correlations revealed that depth(P=0.003),dissolved oxygen(P=0.019),and nitrite(P=0.033)were the main environmental factors affecting bacterial community structure at genus level in the Ninetyeast Ridge.On the other hand,the first two CCA axes accounted for 74.4%of the explained total variance,it seems that the archaeal communities at genus level were heavily influenced by the environmental variables including depth,dissolved oxygen(DO),nitrite,salinity,phosphate,ammonia,nitrate,and silicate,but none of them exhibited any significant correlation on the structuring(P>0.1).
基金The Xiamen Ocean Economic Innovation and Development Demonstration Project under contract No.16PZP001SF16
文摘As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.
文摘The present status of deep sea bed mining technology for polymetallic nodules has been critically examined in light of its history of development the UNCLoS (the United Nation Convention on the Law of the Sea) regime and the current trends in research.The critical technologies have been highlighted and the need for system integration underscored. The deep sea bed environment and the engineering challenge were presented and the necessary features of the deep sea bed mining system for commercial mining was analyzed. The key accomplishments, problems and future bends in research in the development of the mining system are also discussed. Finally, it points out that the technology gaps can be filled by the bine commercial mining is undertaken as a result of market conditions in its favor.
基金Funding Sources:Marine 863 Project (No.2009AA093401)Projects of the National Natural Science Foundation of China (Nos.41072152,90814011 and 41190072)
文摘The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the 'Beyond Plate Tectonics Theory' where a combination of conceptual models and numerical modeling on plume tectonics and plate tectonics has enabled new insights into the structural and tectonic architecture and processes in the deep interior and deep sea. This paper synthesizes developments of structural geology and tectonics from a macroscopic perspective in deep interior and deep sea. Four key techniques are also reviewed: satellite altimetry for surface structures in deep-sea multi-beam sea-floor mapping; tomography for tectonics of the deep interior; diverse modeling approaches and software for unfolding dynamic evolution; and techniques for HT/HP experiments on material rheology and in situ component measurements.
文摘The National Oceanic and Atmospheric Administration reports a 95% decline in the oldest Arctic ice over the last 33 years [1], while the National Aeronautics and Space Administration states that summer Arctic Sea Ice Extent (SIE) is shrinking by 12.2% per decade since 1979 due to warmer temperatures [2]. Given the rapidly changing Arctic conditions, accurate prediction models are crucial. Deep learning models developed for Arctic forecasts primarily focus on exploring convolutional neural networks (CNN) and convolutional Long Short-Term Memory (LSTM) networks, while the exploration of the power of LSTM networks is limited. In this research, we focus on enhancing the performance of an LSTM network for predicting monthly Arctic SIE. We leverage five climate and atmospheric variables, validated for their correlation with SIE in prior studies [3]. We utilize the Spearman’s rank correlation and ExtraTrees regressor to enhance our understanding of the importance of the five variables in predicting SIE. We further enhance our predictor variables with seasonal information, lagged time steps, and a linear regression simulated SIE that accounts for the influence of past SIE on current SIE. Statistical methods guide our selection of data scalers and best evaluation metrics for our model. By experimenting with hyperparameter optimization and advanced deep learning training techniques, such as batch sizes, number of neurons, early stopping, and model checkpoint, our model achieved a Mean Absolute Error (MAE) of 0.191 and R2 of 0.996, underscoring its ability to account for nearly all the variance in our data and holds great promise for the prediction of SIE.
基金Supported by the Senior User Project of R/V Kexue(No.KEXUE2020GZ01)the National Natural Science Foundation of China(No.42176114)。
文摘Solemyidae is an ancient group of protobranch bivalves,and most solemyids are symbiotic with chemoautotrophic and gill-hosted bacteria,enabling them to survive in unusual habitats such as deepsea chemosynthetic environments.However,evolution of the mitogenomes in this family and their phylogenetic relationships remain poorly understood.The complete mitogenome of Acharax sp.was determined and compared with other available mitogenomes of solemyids.The mitogenome of Acharax sp.is 18970 bp in length and consists of 13 protein-coding genes,2 ribosomal RNA genes,and 22 transfer RNA genes.The gene arrangement was identical to those of other sequenced solemyids.For the present five mitogenomes of Solemyidae species,all protein-coding genes were initiated with the typical ATD(ATA,ATG,and ATT)codon and terminated with the TAA/TAG codon.Furthermore,the incomplete termination codon was detected.The Ka/Ks ratio analyses indicated that 13 protein-coding genes of five Solemyidae mitogenomes suffered strong purifying selection.Compared to 45 existing shallow water equivalents,the 18 available mitogenomes from the deep-sea,including the Acharax sp.in this study,show significantly more nonpolar amino acids in the 13 protein-coding genes,which indicates the adaptation to the deep-sea environment.The phylogenetic tree based on 48 Bivalvia complete mitogenomes provided further information to support the scientific classification of protobranchs.The relationships among Solemyidae were assessed based on 2 mitochondrial(16S rRNA and COX1)and 3 nuclear(18S rRNA,28S rRNA,and histone H3)gene sequences from 17 in-group species.The two genera Acharax and Solemya formed a monophyletic clade each,and Acharax sp.clustered with previously reported Acharax bivalves with high support values.
基金The Major State Basic Research Development Program (973 Program) under contract No. 2009CB219402
文摘Drilling wells reveal that the organic matter abundance of Miocene marine source rocks in shallow water area of the Qiongdongnan Basin is relatively low with poor hydrocarbon generation poten- tial. However, in some drilling wells of deep water area close to the central depression belt, Miocene marine source rocks with better organic matter abundance and hydrocarbon generation have been found, which have achieved better source rock standard based on the analysis of geochemical charac- teristics. Although there are no exploratory wells in deep water area of the research region, through the comparative analysis of geochemical data of several typical exploratory wells respectively from shallow water area in the basin, central depression belt margin in deep-water area of the basin and Site 1148 of deep sea drilling in the South China Sea Basin, it reveals that the tendency of the quality of source rocks becomes positive gradually from delta to bathyal environment, which then becomes negative as in deep oceanic environment. Owing to the lack of terrestrial organic matter input, the important controlling factors of Miocene marine source rocks in the Qiongdongnan Basin are ocean productivity and preservation conditions of organic matter. The element geochemistry data indicate that the tendency of the paleoproductivity and the preservation conditions of organic matter become positive as water depth increase from shallow area to bathyal area close to central depression belt. So it is speculated that there must exist high quality source rocks in the central depression area where the preservation conditions of organic matter are much better. Besides, in theory, in oxygen-poor zone of oceanic environment at the water depth 400–1 000 m, the preservation conditions of organic matter are well thus forming high-quality marine source rocks. The result- ing speculation, it is reasonable to consider that there are high hydrocarbon generation potential source rocks in bathyal environment of the Qiongdongnan Basin, especially at the water depth 400– 1 000 m.
基金Supported by the National Major Scientific Instruments Development Project of the National Natural Science Foundation of China(No.41427803)the Zhuang Fa Yu Yan Program(No.41421020401)
文摘A metal-dissolved oxygen seawater battery(SWB)uses metal and dissolved oxygen as the reactants,and it is ideal for use as a long-time low-power distributed power supply in deep sea,due to its advantages of open structure in service without electrolyte.However,several simulating deep-sea environmental factors,such as flow rate,dissolved oxygen concentration,and temperature of seawater may af fect the oxygen reduction reaction(ORR)rate and the stability of electrochemically modified polyacrylonitrile-based carbon fiber brush(MPAN-CFB)cathode,which was studied by steady-state polarization and galvanostatic discharge methods.In addition,the scales formed on MPAN-CFB surface were characterized by SEM and XRD.Results show that the ORR rate increased quickly with the increase of the seawater flow rate up to 3 cm/s,and then gradually stabilized.Moreover,the ORR rate was largely af fected by dissolved oxygen concentration,and the concentration of>3 mg/L was favorable.Compared with surface layer temperature of 15℃,the low temperature of deep sea(4℃)has a negligible ef fect on ORR rate.When the working current is too high,it will lead to the formation of CaCO_3 scales(aragonite)of at the cathodic surface,resulting in the decrease of ORR rate,and consequently the damage to the long-time stability of MPAN-CFB.