Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscor...Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscorea deltoidea Wall var. orbiculata. The new compound was identified to be isonarthogenin 3 O - a - L - rhamnopyranosyl (12) - [ a - L - rhamnopyranosyl - ( 1 4 ) ] - b - D - glucopyranoside by various NMR techniques in combination with chemical methods. The three saponins showed strong activity against Pyricularia oryzae, and were cytotoxic to cancer cell line K562, HCT-15, A549, and A2780a in vitro.展开更多
Ficus deltoidea Jack （Moraceae） or Mas Cotek is a small shrub with a great morphological variation. Measurement of 40 morphological traits had been done on 50 accessions to find the most significant characters that ...Ficus deltoidea Jack （Moraceae） or Mas Cotek is a small shrub with a great morphological variation. Measurement of 40 morphological traits had been done on 50 accessions to find the most significant characters that enable differentiation being done according to its variety groups. The data were analyzed with principal component analysis （PCA） and cluster analysis （CA） using cluster software package programme to produce the scatter diagram and dendrogram relationship of the taxa. The results showed that there were 25 morphological characters having the value of factor analysis greater than 0.60 from its principal component （PC） with the Eigen value greater than 1.0. 16 out of 40 morphological characters had been identified as having high values of correlation coefficient ranging from -0.783 to 0.890. The analysis showed that the most responsible characters in grouping the samples into different groups are the shape and size of leaf, number and color of dots on the leaf surface and characters of syconium. The scatter diagram of the accessions on the PC1 against PC2 showed six major groups. The dendrogram displayed the relationship among the accessions and within the dissimilarity distance = 19, it classified the samples into five major groups. Observation on F. deltoidea resulted in the findings of high variability among the accessions. The most significant characters in grouping accessions are the shapes of leaf base （BL）, shape of leaf apex （SA）, ratio of lamina width to lamina length （R）, dots color at the lower midrib （DLM）, color of young syconium （CYS）, color of mature syconium （CMS） and the number of syconium on trees （DST）. This study provides basic information for introduction of some particular traits and effective conservation of the species breeding programme. The morphological traits were found to be useful for the diversity studies and in identifying the variation. The actual figures of F. deltoidea obtained through this study enable comparison being done to the previous and in future study. Hence, the varieties that are extinct could be recognised.展开更多
Objective： This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods： Methanol and water extracts of l...Objective： This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods： Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content （TFC） and total phenolic content （TPC）, respectively. The study was initiated by determining the maximum non-toxic dose （MNTD） of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD （1/2MNTD）, after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density （OD） at 520 nm, respec- tively. Results： The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents （QE）/100 g dry weight （DW）, while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents （GAE）/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were （300.0±28.3） and （225.0±21.2） μg/ml, respectively, for var. deltoidea, while much lower MNTDs [（60.0±2.0） IJg/ml for methanol extracts and （8.0＋1.0） μg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or 1/2MNTD significantly inhibited the maturation of preadipocytes. Conclusions： The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.展开更多
文摘Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscorea deltoidea Wall var. orbiculata. The new compound was identified to be isonarthogenin 3 O - a - L - rhamnopyranosyl (12) - [ a - L - rhamnopyranosyl - ( 1 4 ) ] - b - D - glucopyranoside by various NMR techniques in combination with chemical methods. The three saponins showed strong activity against Pyricularia oryzae, and were cytotoxic to cancer cell line K562, HCT-15, A549, and A2780a in vitro.
文摘Ficus deltoidea Jack （Moraceae） or Mas Cotek is a small shrub with a great morphological variation. Measurement of 40 morphological traits had been done on 50 accessions to find the most significant characters that enable differentiation being done according to its variety groups. The data were analyzed with principal component analysis （PCA） and cluster analysis （CA） using cluster software package programme to produce the scatter diagram and dendrogram relationship of the taxa. The results showed that there were 25 morphological characters having the value of factor analysis greater than 0.60 from its principal component （PC） with the Eigen value greater than 1.0. 16 out of 40 morphological characters had been identified as having high values of correlation coefficient ranging from -0.783 to 0.890. The analysis showed that the most responsible characters in grouping the samples into different groups are the shape and size of leaf, number and color of dots on the leaf surface and characters of syconium. The scatter diagram of the accessions on the PC1 against PC2 showed six major groups. The dendrogram displayed the relationship among the accessions and within the dissimilarity distance = 19, it classified the samples into five major groups. Observation on F. deltoidea resulted in the findings of high variability among the accessions. The most significant characters in grouping accessions are the shapes of leaf base （BL）, shape of leaf apex （SA）, ratio of lamina width to lamina length （R）, dots color at the lower midrib （DLM）, color of young syconium （CYS）, color of mature syconium （CMS） and the number of syconium on trees （DST）. This study provides basic information for introduction of some particular traits and effective conservation of the species breeding programme. The morphological traits were found to be useful for the diversity studies and in identifying the variation. The actual figures of F. deltoidea obtained through this study enable comparison being done to the previous and in future study. Hence, the varieties that are extinct could be recognised.
基金Project supported by the International Medical University （No. BPharm B0108 Res072011）, Malaysia
文摘Objective： This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods： Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content （TFC） and total phenolic content （TPC）, respectively. The study was initiated by determining the maximum non-toxic dose （MNTD） of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD （1/2MNTD）, after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density （OD） at 520 nm, respec- tively. Results： The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents （QE）/100 g dry weight （DW）, while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents （GAE）/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were （300.0±28.3） and （225.0±21.2） μg/ml, respectively, for var. deltoidea, while much lower MNTDs [（60.0±2.0） IJg/ml for methanol extracts and （8.0＋1.0） μg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or 1/2MNTD significantly inhibited the maturation of preadipocytes. Conclusions： The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.
