Bladder,kidney,prostate and testicular carcinoma are the top four genitourinary cancers in China.Here we analyzed mRNA and miRNA expression profiles of carcinomas of the bladder(TCC),kidney(ccRCC)and testis(TGCT)to un...Bladder,kidney,prostate and testicular carcinoma are the top four genitourinary cancers in China.Here we analyzed mRNA and miRNA expression profiles of carcinomas of the bladder(TCC),kidney(ccRCC)and testis(TGCT)to uncover their specific regulatory mechanisms.The gene expression profiles of GSE31617 were downloaded from GEO database,which contained 27 samples,including 10 TCC,7 TGCT and 10 ccRCC.Specific up-and downregulated differentially expressed genes(DEGs)and differentially expressed microRNAs(DEmiRNAs)of each cancer were selected and target genes of DEmiRNAs were predicted.Gene interaction network of the shared genes and target genes of DEmiRNAs of each cancer was predicted by STRING and constructed by Cytoscape.In each cancer,we build regulatory networks of hub genes selected and conducted GO analysis of enriched genes.Furthermore,we chose four hub genes(SALL4,RHEB,CDC42 and TNN)for survival analysis in OncoLnc database,and they all had effects on the survival rate of another genitourinary cancer-kidney renal clear cell carcinoma(KIRC).In conclusion,the present study indicated that the identified hub genes promote our understanding of molecular mechanisms underlying the development of three genitourinary cancers,and might be used as molecular targets and diagnostic biomarkers for the treatment of them.展开更多
文摘Bladder,kidney,prostate and testicular carcinoma are the top four genitourinary cancers in China.Here we analyzed mRNA and miRNA expression profiles of carcinomas of the bladder(TCC),kidney(ccRCC)and testis(TGCT)to uncover their specific regulatory mechanisms.The gene expression profiles of GSE31617 were downloaded from GEO database,which contained 27 samples,including 10 TCC,7 TGCT and 10 ccRCC.Specific up-and downregulated differentially expressed genes(DEGs)and differentially expressed microRNAs(DEmiRNAs)of each cancer were selected and target genes of DEmiRNAs were predicted.Gene interaction network of the shared genes and target genes of DEmiRNAs of each cancer was predicted by STRING and constructed by Cytoscape.In each cancer,we build regulatory networks of hub genes selected and conducted GO analysis of enriched genes.Furthermore,we chose four hub genes(SALL4,RHEB,CDC42 and TNN)for survival analysis in OncoLnc database,and they all had effects on the survival rate of another genitourinary cancer-kidney renal clear cell carcinoma(KIRC).In conclusion,the present study indicated that the identified hub genes promote our understanding of molecular mechanisms underlying the development of three genitourinary cancers,and might be used as molecular targets and diagnostic biomarkers for the treatment of them.
