In order to understand the pathogenicity of duck Tembusu virus （DTMUV）, it was injected into muscle of 5-d-old Cherry Valley ducklings according to the dosage of 1×104 EID50. Then, the biochemical indexes of du...In order to understand the pathogenicity of duck Tembusu virus （DTMUV）, it was injected into muscle of 5-d-old Cherry Valley ducklings according to the dosage of 1×104 EID50. Then, the biochemical indexes of duckling serum samples were determined by kits, and the changes in detoxification, tissue viral load and cytokines were detected by using fluorescence quantitative PCR. The results showed that DTMUV had serious damage to the liver, kidney, heart and muscle of ducklings; DTMUV could proliferate in the liver, spleen, lung and brain; the virus levels in the liver and brain reached the peaks on day 5 after the inoculation and those in the lung and spleen reached the peaks on day 9; the virus content was highest in the brain, liver and spleen; and DTMUV induced the overexpression of IFN-γ, IFN-α, IL-6, IFN-β, IL-1β, TLR-7,IL-2, major histocompatibility complex type I （MHC-I） andmajor histocompatibility complex type II （MHC-II） in the spleen on day 1 and the overexpression of IL-6 and IL-2 in the brain on days 1, 2 and 3.展开更多
Duck tembusu virus disease is one of the most serious infectious diseases endangering duck industry. A strain of virus was isolated from a dead duck,and performed PCR identification and sequence analysis. The results ...Duck tembusu virus disease is one of the most serious infectious diseases endangering duck industry. A strain of virus was isolated from a dead duck,and performed PCR identification and sequence analysis. The results showed that the sequence of the isolate shared above 99% homology with duck tembusu virus( DTMUV) sequence on Gen Bank. The result indicated that the isolated virus was DTMUV.展开更多
Eleven strains of duck Tembusu virus(TMUV)were isolated from diseased ducks at different duck farms in South China during 2011–2015,and their whole genomes were sequenced.The 11 isolated strains shared high sequence ...Eleven strains of duck Tembusu virus(TMUV)were isolated from diseased ducks at different duck farms in South China during 2011–2015,and their whole genomes were sequenced.The 11 isolated strains shared high sequence identity from 95.9%to 99.5%.Phylogenetic analysis revealed that all TMUV strains isolated after 2010 are clustered into two distinct branches,one branch comprising 2 Malaysian strains,and the other branch comprising all Chinese and Thai strains forming Chinese cluster 1 and Chinese cluster 2.Five of the 11 isolated strains were closely related to classical Chinese strains(BYD)belonging to Chinese cluster 1,and the remaining 6 isolated strains were closely related to strains newly isolated in South China and Thailand,belonging to Chinese cluster 2(a mixed China-Thailand cluster).Phylogenetic analysis of the partial E and NS 5 gene of TMUVs also showed the similar results.The results collectively showed a new trend of TMUV disease that two Chinese clusters of TMUV have been concurrently circulating in South China.This study provides practical guidance for preparing vaccines against TMUV in South China.展开更多
基金Supported by National Natural Science Foundation of China(31402224)Key Research&Development Project of Hainan Province(ZDYF2017029)Agricultural Science&Technology Innovation Fund of Hainan Academy of Agricultural Sciences(CXZX201413)~~
文摘In order to understand the pathogenicity of duck Tembusu virus （DTMUV）, it was injected into muscle of 5-d-old Cherry Valley ducklings according to the dosage of 1×104 EID50. Then, the biochemical indexes of duckling serum samples were determined by kits, and the changes in detoxification, tissue viral load and cytokines were detected by using fluorescence quantitative PCR. The results showed that DTMUV had serious damage to the liver, kidney, heart and muscle of ducklings; DTMUV could proliferate in the liver, spleen, lung and brain; the virus levels in the liver and brain reached the peaks on day 5 after the inoculation and those in the lung and spleen reached the peaks on day 9; the virus content was highest in the brain, liver and spleen; and DTMUV induced the overexpression of IFN-γ, IFN-α, IL-6, IFN-β, IL-1β, TLR-7,IL-2, major histocompatibility complex type I （MHC-I） andmajor histocompatibility complex type II （MHC-II） in the spleen on day 1 and the overexpression of IL-6 and IL-2 in the brain on days 1, 2 and 3.
基金Supported by National Natural Science Foundation of China(31402224)Key Research & Development Project of Hainan Province(ZDYF2017029)Agricultural Science and Technology Innovation Project of Hainan Academy of Agricultural Sciences(CXZX201413)
文摘Duck tembusu virus disease is one of the most serious infectious diseases endangering duck industry. A strain of virus was isolated from a dead duck,and performed PCR identification and sequence analysis. The results showed that the sequence of the isolate shared above 99% homology with duck tembusu virus( DTMUV) sequence on Gen Bank. The result indicated that the isolated virus was DTMUV.
基金Supported by the Science and Technology Planning Project of Guangdong Province,China(2017A040403015,2017A020225022)the Natural Science Foundation of Guangdong Province,China(2018B030315002)the Guangdong Province Key Laboratory of Waterfowl Healthy Breeding Open Funding(SQZS1802)
文摘Eleven strains of duck Tembusu virus(TMUV)were isolated from diseased ducks at different duck farms in South China during 2011–2015,and their whole genomes were sequenced.The 11 isolated strains shared high sequence identity from 95.9%to 99.5%.Phylogenetic analysis revealed that all TMUV strains isolated after 2010 are clustered into two distinct branches,one branch comprising 2 Malaysian strains,and the other branch comprising all Chinese and Thai strains forming Chinese cluster 1 and Chinese cluster 2.Five of the 11 isolated strains were closely related to classical Chinese strains(BYD)belonging to Chinese cluster 1,and the remaining 6 isolated strains were closely related to strains newly isolated in South China and Thailand,belonging to Chinese cluster 2(a mixed China-Thailand cluster).Phylogenetic analysis of the partial E and NS 5 gene of TMUVs also showed the similar results.The results collectively showed a new trend of TMUV disease that two Chinese clusters of TMUV have been concurrently circulating in South China.This study provides practical guidance for preparing vaccines against TMUV in South China.
