Ecological water replenishment(EWR)is an important strategy for river restoration globally,but timely evaluation of its ecological effects at a large spatiotemporal scale to further adjust the EWR schemes is of great ...Ecological water replenishment(EWR)is an important strategy for river restoration globally,but timely evaluation of its ecological effects at a large spatiotemporal scale to further adjust the EWR schemes is of great challenge.Here,we examine the impact of EWR on microeukaryotic plankton communities in three distinct river ecosystems through environmental DNA(eDNA)metabarcoding.The three ecosystems include a long-term cut-off river,a short-term connected river after EWR,and long-term connected rivers.We analyzed community stability by investigating species composition,stochastic and deterministic dynamics interplay,and ecological network robustness.We found that EWR markedly reduced the diversity and complexity of microeukaryotic plankton,altered their community dynamics,and lessened the variation within the community.Moreover,EWR disrupted the deterministic patterns of community organization,favoring dispersal constraints,and aligning with trends observed in naturally connected rivers.The shift from an isolated to a temporarily connected river appeared to transition community structuring mechanisms from deterministic to stochastic dominance,whereas,in permanently connected rivers,both forces concurrently influenced community assembly.The ecological network in temporarily connected rivers post-EWR demonstrated significantly greater stability and intricacy compared to other river systems.This shift markedly bolstered the resilience of the ecological network.The eDNA metabarcoding insights offer a novel understanding of ecosystem resilience under EWR interventions,which could be critical in assessing the effects of river restoration projects throughout their life cycle.展开更多
基金supported by the National Key Research and Development Program of China(2021YFC3201005,2021YFC3201000,and 2022YFC2601301)the Fundamental Research Funds for Central Public Welfare Scientific Research Institutes of China(2022YSKY-41)+1 种基金the National Nature Science Foundation of China(U1906223)The authors thank Yong Du in Yongding River Investment Co.Ltd for providing Fig.S2.Dr James Walter Voordeckers is acknowledged for assistance in English language modification.
文摘Ecological water replenishment(EWR)is an important strategy for river restoration globally,but timely evaluation of its ecological effects at a large spatiotemporal scale to further adjust the EWR schemes is of great challenge.Here,we examine the impact of EWR on microeukaryotic plankton communities in three distinct river ecosystems through environmental DNA(eDNA)metabarcoding.The three ecosystems include a long-term cut-off river,a short-term connected river after EWR,and long-term connected rivers.We analyzed community stability by investigating species composition,stochastic and deterministic dynamics interplay,and ecological network robustness.We found that EWR markedly reduced the diversity and complexity of microeukaryotic plankton,altered their community dynamics,and lessened the variation within the community.Moreover,EWR disrupted the deterministic patterns of community organization,favoring dispersal constraints,and aligning with trends observed in naturally connected rivers.The shift from an isolated to a temporarily connected river appeared to transition community structuring mechanisms from deterministic to stochastic dominance,whereas,in permanently connected rivers,both forces concurrently influenced community assembly.The ecological network in temporarily connected rivers post-EWR demonstrated significantly greater stability and intricacy compared to other river systems.This shift markedly bolstered the resilience of the ecological network.The eDNA metabarcoding insights offer a novel understanding of ecosystem resilience under EWR interventions,which could be critical in assessing the effects of river restoration projects throughout their life cycle.
