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兽药恩诺沙星(enrofloxacin)的水解特性 被引量:21
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作者 吴银宝 廖新俤 +2 位作者 汪植三 陈杖榴 周阳 《应用生态学报》 CAS CSCD 北大核心 2006年第6期1086-1090,共5页
研究了恩诺沙星在不同pH、不同光照及不同微生物条件下的水解,为其生态风险评价提供依据.结果表明,恩诺沙星的水解产物中没有环丙沙星;50 ℃时,避光5 d后恩诺沙星在pH 1~10缓冲液中的水解都小于10%,表明恩诺沙星在恒温避光下的水解半... 研究了恩诺沙星在不同pH、不同光照及不同微生物条件下的水解,为其生态风险评价提供依据.结果表明,恩诺沙星的水解产物中没有环丙沙星;50 ℃时,避光5 d后恩诺沙星在pH 1~10缓冲液中的水解都小于10%,表明恩诺沙星在恒温避光下的水解半衰期将超过1年,同时溶液pH值的变化对恩诺沙星的水解速率无显著影响;恩诺沙星在天然水中的降解与光照有关,在室外自然光照条件下恩诺沙星降解很快,3 d后水中已经检测不出恩诺沙星.在室内自然光下,恩诺沙星降解较慢,在初始浓度分别为0.05、0.2、1.0 mg·L^-1条件下,31 d试验期内分别降解了48%、72%和65%;在避光条件下,恩诺沙星非常稳定,不易降解.在不同初始浓度下,微生物对恩诺沙星的水解无显著影响.由于恩诺沙星在室外自然光照条件下会迅速降解,因此不会对水环境构成直接的危害,但不能忽视其潜在的生态风险. 展开更多
关键词 恩诺沙星 水解
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Pharmacokinetics and tissue behavior of enrofloxacin and its metabolite ciprofloxacin in turbot Scophthalmus maximus at two water temperatures 被引量:13
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作者 梁俊平 李健 +2 位作者 赵法箴 刘萍 常志强 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第4期644-653,共10页
Turbot Scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fis... Turbot Scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fish infections. However, studies concerning the pharmacokinetics of enrofloxacin in turbot are limited. In this study, the pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin, were investigated in the turbot following intravenous and oral administration at 10 mg enrofloxacin/kg body weight, at 16℃ and 10℃ water temperatures. The concentrations of enrofloxacin and ciprofloxacin in the main tissues (plasma, muscle, liver and kidney) were detected by HPLC. The results show that the plasma concentration-time data for enrofloxacin were best described as a two-compartment open model after intravenous and oral administration. Three pharmacokinetic equations were established between the concentrations and temperatures. The kinetic profile of enrofloxacin was temperature dependent. The absorption half-life of enrofloxacin was 1.99 h and 2.17 h after oral administration, whereas the elimination half-life of the drug was 98.63 h and 136.59 h at 16℃ and 10℃, respectively. The peak concentration of enrofloxacin in plasma and tissues was higher at 16℃ than that at 10℃, and the peak plasma concentration time in the liver was the shortest at both temperatures among those of other tissues. The plasma ℃/MIC ratio varied between 11.08 and 5 540.00 at 16℃; and between 7.92 and 3 960.00 at 10℃. The AUC/MIC ratio was 467.82-280 690.00 at 16℃, and 359.48-215 690.00 at 10℃. These ratios indicate that it is possible to obtain therapeutic efficacy. Very low levels of ciprofloxacin were detected. The AUC ratios of ciprofloxacin and enrofloxacin in plasma suggest that plasma ciprofloxacin might play a minor role in enrofloxacin treatment for turbot. 展开更多
关键词 Scophthalmus maximus enrofloxacin CIPROFLOXACIN PHARMACOKINETICS
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Inhibition of Breast Cancer Resistance Protein(BCRP) by Ko143 Can Affect Pharmacokinetics of Enrofloxacin in Exopalaemon carinicauda 被引量:2
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作者 ZHAI Qianqian XU Yang +4 位作者 LI Cuiping FENG Yanyan CUI Yanting MA Li LI Jian 《Journal of Ocean University of China》 SCIE CAS CSCD 2020年第5期1116-1124,共9页
Adenosine triphosphate-binding cassette transporter breast cancer resistance protein(BCRP) exists highly in the apical membranes of epithelia, and is involved in drug availability. Ko143 is a typical inhibitor of BCRP... Adenosine triphosphate-binding cassette transporter breast cancer resistance protein(BCRP) exists highly in the apical membranes of epithelia, and is involved in drug availability. Ko143 is a typical inhibitor of BCRP in rodents. The synthetic antibacterial agent enrofloxacin(ENRO) is a fluoroquinolone employed as veterinary and aquatic medicine, and also a substrate for BCRP. BCRP gene highly expressed in the hepatopancreas and intestine of Exopalaemon carinicauda as was determined with real-time quantitative reverse transcription-polymerase chain reaction(RT-q PCR) method. The effects of Ko143 on the abundance of BCRP m RNA and ENRO pharmacokinetics in E. carinicauda were studied. The m RNA abundance of BCRP decreased significantly in hepatopancreas and intestine(P < 0.05) after Ko143 treatment. Co-administration of Ko143 significantly changed the pharmacokinetics of orally administered enrofloxacin, which was supported by higher distribution half-life(t_(1/2α)), elimination half-life(t_(1/2β)), area under the curve up to the last measurable concentration(AUC_(0-t)), peak concentration(C_(max)) and lower clearance(CL/F). These findings revealed that Ko143 downregulates BCRP expression in hepatopancreas and intestine, thus affects the pharmacokinetics of orally administered enrofloxacin in E. carinicauda. The drug-drug interaction can be caused by the change in BCRP activity if ENRO is used in combination with other drugs in shrimp. 展开更多
关键词 BCRP Exopalaemon carinicauda PHARMACOKINETICS enrofloxacin Ko143
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Anti-enrofloxacin Antibody Production by Using Enrofloxacin-screened HSA as an Immunogen 被引量:2
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作者 LIU Chune LIN Hong CAO Limin JIANG Jie 《Journal of Ocean University of China》 SCIE CAS 2005年第3期262-266,共5页
A two-step zero-length cross-linking procedure using active esters was successfully adopted for conjugating en-rofloxacin (EF) to human serum albumin (HSA). The derived conjugate was characterized by UV spectrum and t... A two-step zero-length cross-linking procedure using active esters was successfully adopted for conjugating en-rofloxacin (EF) to human serum albumin (HSA). The derived conjugate was characterized by UV spectrum and then usedfor immunization of BALB/C mice. In enzyme-linked immunosorbent assay (ELISA) and competitive inhibition ELISA ex-periments, the derived antiserum exhibited high antibody titer (greater than 1: 250 000) as well as varied cross-reactivity(from 97.8% to 161.7%) to three analogs of EF belonging to fluoroquinolones family. But over the concentration rangestudied, no significant cross-reactivity was observed to other group of antibiotics (chloramphenicol, oxytetracycline, sul-phamethoxazole and nysfungin). It was confirmed that the synthesized immunogen was highly antigenic and elicited specificantibody responses in BALB/C mice against EF. 展开更多
关键词 enrofloxacin IMMUNOGEN enzyme-linked immunosorbent assay human serum albumin
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A Review of Enrofloxacin for Veterinary Use 被引量:5
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作者 Tessa Trouchon Sébastien Lefebvre 《Open Journal of Veterinary Medicine》 2016年第2期40-58,共19页
This review outlines the current knowledge on the use of enrofloxacin in veterinary medicine from biochemical mechanisms to the use in the field conditions and even resistance and ecotoxicity. The basics of biochemist... This review outlines the current knowledge on the use of enrofloxacin in veterinary medicine from biochemical mechanisms to the use in the field conditions and even resistance and ecotoxicity. The basics of biochemistry, the mechanisms of action and resistance and pharmacokinetics are presented. Then an overview of available veterinary products, their efficacy and their toxicity against target species, human and environment is provided. 展开更多
关键词 enrofloxacin Antibiotic Resistances VETERINARY
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Pharmacokinetics of Enrofloxacin and Its Metabolite in Carp (Cyprinus carpio) After a Single Oral Administration in Medicated Feed 被引量:1
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作者 LIU Ying LI Zhaoxin +6 位作者 ZHANG Dahai XING Lihong SUN Weihong SUN Xiaojie PENG Jixing ZHANG Yonggang LI Xianguo 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第1期171-180,共10页
A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CI... A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CIP)in carp tissues.Optimized chromatographic separation was obtained on a Waters Xterra MS C_(18) reversed-phase column using gradient elution with methanol and 0.1%formic acid aqueous solution including 5mmolL^(-1) of ammonium acetate.The established method was applied to study the pharmacokinetics and distribution of ENR and CIP in tissues of carp following a single oral administration in feed at a dosage of 40mgkg^(-1) bw(body weight).Data were analyzed using DAS 2.0 dynamics software,and the experimental results suggest that ENR was rapidly absorbed and extensively distributed in carp tissues through systemic circulation,and the pharmacokinetic characteristics can be described with a two-compartment model.The elimination half-lives(t_(1/2β))from muscle,liver,gill,plasma and skin were 131,160,104,132 and 310 h,respectively.The areas under the drug concentration-time curves(AUC)for these tissues were 491,972,750,249 and 706hmgkg^(-1),respectively.The maximum concentration(C_(max))values were 13,29,37,9 and 5mgkg^(-1) with peak times(t_(max))of 8,4,4,2 and 4 h,respectively.Ciprofloxacin,the active metabolite of ENR,was also detected in carp tissues,indicating that only 1.54%of de-ethylation of ENR occurs in carp.At a water temperature of 18℃,the drug withdrawal time was determined to be no less than 24 d while the carp was fed at a single dosage of 40mgkg^(-1). 展开更多
关键词 Cyprinus carpio enrofloxacin CIPROFLOXACIN PHARMACOKINETICS liquid chromatography-tandem mass spectrometry
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Studies on Preparation and Characteristics of Monoclonal Antibodies Against Enrofloxacin and Cross-Reactivity of Related Fluoroquinolones
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作者 CAIQin-ren ZENGZhen-ling YANGGui-xiang CHENZhang-liu 《Agricultural Sciences in China》 CAS CSCD 2004年第11期862-868,共7页
An ester activation method was employed to couple enrofloxacin(ENFX) to the carrier proteins BSA and OVA. The conjugates ENFX-BSA and ENFX-OVA were identified with an UV spectrophotometer and amino acid automation a... An ester activation method was employed to couple enrofloxacin(ENFX) to the carrier proteins BSA and OVA. The conjugates ENFX-BSA and ENFX-OVA were identified with an UV spectrophotometer and amino acid automation analysis instrument, and resulted in conjugates with 48 ENFX molecules per carrier molecule(BSA). Splenocytes from mice immunized with ENFX-BSA were fused with SP2/0 myeloma cells and hybridomas secreting antibodies against enrofloxacin were selected and cloned. Two stable monoclonal antibodies, 2C5, 5D5 of the subclass IgG2a, were isolated. Using antibody 5D5, an indirect competitive inhibition enzyme-linked immunosorbent assay (Ci-ELISA) was developed for the quantitative detection of enrofloxacin and its metabolites. The IC50 of the standard curve was 21.67 ng mL-1 and the limit of detection for enrofloxacin was 0.13 ng mL-1. This method was sensitive and had a linear range from 0.13 to 10 000 ng mL-1 (r= -0.9782). Monoclonal antibody 5D5 exhibited high relative affinity to enrofloxacin, and the cross-reactivities with ciprofloxacin, marbofloxacin, sarafloxacin and danorfloxacin were 110.8, 27.40, 71.05 and 37.41%, respectively. Three non-fluoroquinolones of cefadroxil, chloramphenicol, sulfadimethoxine were tested and there was no cross-reaction between them. 展开更多
关键词 enrofloxacin Monoclonal antibodies CROSS-REACTIVITY
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Concentration of Enrofloxacin Residue from Tilapia (Oreochromis niloticus) Muscular That Infected by Aeromonas salmonicida
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作者 Kumiasih Fera Aryanti 《Journal of Agricultural Science and Technology(A)》 2014年第9期750-754,共5页
Aim of this research was to find out the concentration of enrofloxacin residue in tilapia meat for several weeks after antibiotic treatment. Twenty seven tilapia fishes were divided into three groups. The first group ... Aim of this research was to find out the concentration of enrofloxacin residue in tilapia meat for several weeks after antibiotic treatment. Twenty seven tilapia fishes were divided into three groups. The first group was not infected and treated, the second group was infected with A. salmonicida subsp, smithia and the third group was infected with A. salmonicida subsp. achromogenes intramuscularly. Six days after infection, treatment was carried out using Baytril administered orally for the second group and intramuscularly for the third group during five days. At the 1 st, 4th and 8th week after the treatment, Three fish were taken from each group to be analyzed for its concentration of enrofloxacin residue by diffusion on Mueller Hinton Agar (MHA) method and quantitatively using high performance liquid chromatography (HPLC) method. The MHA test showed the formation of inhibition zone, at the 1 st week and 4th week after the treatment, while at 8th week after treatment did not show inhibition zone. The HPLC test on enrofloxacin residual concentration in tilapia infected with A. salmonicida subsp, smithia (second group) at the 1st, 4th and 8th week after treatment showed the average of 33.0, 6.10 and 0.0021 μg/g of enrofloxacin residue level. While in tilapia infected with A. salmonicida subsp, achromogenes and treated with enrofloxacin intramuscularly (third group) showed the average of residue level 35.79, 2.18 and 0.00065 μg/g. In conclusion, the residue of enrofloxacin was still high concentration until the fourth week after treatment in the second and third groups. Based on Indonesian National Standards and Rules, the maximum limit of enrofloxacin residue is 0.01 μg/g. The concentration of enrofloxacine residue was very low and the concentration of enrofloxacin residue collected from tilapia using orally and intramuscularly method of treatment was not different. 展开更多
关键词 RESIDUE enrofloxacin Aeromonas salmonicida subsp smithia SUBSP achromogenes.
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含enrofloxacin脂质体制剂
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作者 林红霞 孙淑英 《辽宁医药》 2001年第2期35-36,共2页
关键词 enrofloxacin 脂质体制剂 磷脂量 抗氧剂
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Photocatalytic degradation of enrofloxacin with CoAl-LDH mediated persulfate system:Efficiency evaluations and reaction pathways
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作者 Yuxuan Zhang Kai He +5 位作者 Long Chen Wen Liu c Jinlong Yuan Yaotong Gao Yuanfeng Qi Bingjun Liu 《Emerging Contaminants》 CSCD 2024年第3期280-292,共13页
Residual enrofloxacin(ENR)exposed in aqueous environments is challenging to the ecosphere.In this work,a layered double hydroxide CoAl-LDH was used to activate the common oxidizing agent persulfate(PS)for photodegrada... Residual enrofloxacin(ENR)exposed in aqueous environments is challenging to the ecosphere.In this work,a layered double hydroxide CoAl-LDH was used to activate the common oxidizing agent persulfate(PS)for photodegradation of ENR,and the degradation pathways of ENR were scrutinized and elucidated.The results indicated that,under the optimal conditions obtained through orthogonal experiments,even though the degradation rate of ENR was as high as 97.72%,the removal of total organic carbon(TOC)from the system was only about 30%.Eleven probable reaction pathways were categorized,and thirty-one types of intermediates were identified in participating in the complex degradation process.The major products of ENR were P4(C_(17)H_(20)FN_(3)O_(3)),P22(C_(19)H_(22)FN_(3)O_(4)),P19(C_(17)H_(18)FN_(3)O_(3)),which are mainly derived from the cleavage of the piperazine groups and quinolone rings.Density functional theory(DFT)calculations of the Fukui index for ENR revealed that the two N atoms in the piperazine ring were the core reactive sites in triggering the degradation chains,which were sensitive for electrophilic attack by the dominant radicals(·OH and SO_(4)·^(-))generated from the composite PS-UV-CoAl-LDH system. 展开更多
关键词 Layered double hydroxide PERSULFATE enrofloxacin Degradation pathway Density functional theory
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Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin 被引量:15
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作者 Hai-tang ZHANG Jin-qing JIANG +5 位作者 Zi-liang WANG Xin-yao CHANG Xing-you LIU San-hu WANG Kun ZHAO Jin-shan CHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第11期884-891,共8页
Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide(EDC) method was employed to synthesize the artificial antigen of enrofloxacin(ENR),and New Zealand rabbits were used to produce anti-ENR polyclonal antibody(pAb).... Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide(EDC) method was employed to synthesize the artificial antigen of enrofloxacin(ENR),and New Zealand rabbits were used to produce anti-ENR polyclonal antibody(pAb).Based on the checkerboard titration,an indirect competitive enzyme-linked immunosorbent assay(ELISA) standard curve was established.This assay was sensitive and had a linear range from 0.6 to 148.0 μg/kg(R2=0.9567),with the half maximal inhibitory concentration(IC50) and limit of detection(LOD) values of 9.4 μg/kg and 0.2 μg/kg,respectively.Of all the competitive analogues,the produced pAb exhibited a high cross-reactivity to ciprofloxacin(CIP)(87%),the main metabolite of ENR in tissues.After optimization,the matrix effects can be ignored using a 10-fold dilution in beef and 20-fold dilution in pork.The overall recoveries and coefficients of variation(CVs) were in the ranges of 86%-109% and 6.8%-13.1%,respectively.It can be concluded that the established ELISA method is suitable for simultaneous detection of ENR and CIP in animal tissues. 展开更多
关键词 enrofloxacin CIPROFLOXACIN Indirect competitive ELISA Animal tissues
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Leaching behavior of enrofloxacin in three different soils and the influence of a surfactant on its mobility 被引量:6
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作者 Zhiyong Yu Ayfer Yediler +1 位作者 Min Yang Sigurd Schulte-Hostede 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第3期435-439,共5页
The leaching behaviors of enrofloxacin (ENR), a fluoroquinolone group antibiotic, in three different standard soils, namely sandy, loamy sand and sandy loam were investigated according to OECD guideline 312. In addi... The leaching behaviors of enrofloxacin (ENR), a fluoroquinolone group antibiotic, in three different standard soils, namely sandy, loamy sand and sandy loam were investigated according to OECD guideline 312. In addition, the effects of tenside, sodium dodecylbenzenesulfonate (DBS) on the mobility of ENR in two different soils were studied. The mobility of ENR in all three standard soils was very similar and was mostly (98%) concentrated on the top 0-5 cm segment of the soils at pH 5.7. The DBS can enhance the mobility of ENR in soils but the impact was in general negligible under the studied conditions. 展开更多
关键词 enrofloxacin LEACHING SOIL sodium dodecylbenzenesulfonate
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Toxic effects of enrofloxacin on Scenedesmus obliquus 被引量:4
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作者 Hongwei QIN Liufang CHEN +2 位作者 Nan LU Yahui ZHAO Xing YUAN 《Frontiers of Environmental Science & Engineering》 SCIE EI CAS CSCD 2012年第1期107-116,共10页
In this article, the toxic effects of Enrofloxacin (ENFX) on Scenedesmus obliquus were studied, through investigating the growth, photosynthetic pigments, and protein contents. The possible toxic mechanisms of ENFX ... In this article, the toxic effects of Enrofloxacin (ENFX) on Scenedesmus obliquus were studied, through investigating the growth, photosynthetic pigments, and protein contents. The possible toxic mechanisms of ENFX were analyzed by determining the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, proline content, and superoxide anion (O2) generation rate. Results showed that the growth of algae was inhibited by ENFX and the 50% effective concentration (EC50) values for 24, 48, 72, and 96 h of ENFX were 88.39, 63.86, 45.10, and 59.16 mg .L^-1, respectively. After treated with ENFX for 96h, the contents of photosynthetic pigments decreased with the increase of ENFX concentration, the content of soluble protein and the activity of SOD increased and then decreased, and the generation rate of superoxide anion (O2^-) increased continually. The contents of MDA and proline changed little in lower ENFX concentration groups, but increased rapidly when treated with higher concentration groups. These results suggested that ENFX affected the growth ofS. obliquus, and the main toxicity mechanism was that algal cells generated the reactive oxygen species under ENFX stress, and then the reactive oxygen species (ROS) induced the oxidation damages of biologic macromolecules and changed the biomembrane permeability further. 展开更多
关键词 enrofloxacin Scenedesmus obliquus toxic effects
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Multi-function adsorbent-photocatalyst MXene-TiO_(2) composites for removal of enrofloxacin antibiotic from water 被引量:4
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作者 Siwanat Sukidpaneenid Chamorn Chawengkijwanich +3 位作者 Chonlada Pokhum Toshihiro Isobe Pakorn Opaprakasit Paiboon Sreearunothai 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第2期414-428,共15页
MXenes,a new family of two-dimensional transition metal carbides or nitrides,have attracted tremendous attention for various applications due to their unique properties such as good electrical conductivity,hydrophilic... MXenes,a new family of two-dimensional transition metal carbides or nitrides,have attracted tremendous attention for various applications due to their unique properties such as good electrical conductivity,hydrophilicity,and ion intercalability.In this work,Ti_(3)C_(2) MXene,or MX,is converted to MX-TiO_(2) composites using a simple and rapid microwave hydrothermal treatment in HCl/NaCl mixture solution that induces formation of fine TiO_(2) particles on the MX parent structure and imparts photocatalytic activity to the resulting MX-TiO_(2) composites.The composites were used for enrofloxacin(ENR),a frequently found contaminating antibiotic,removal from water.The relative amount of the MX and TiO_(2) can be controlled by controlling the hydrothermal temperature resulting in composites with tunable adsorption/photocatalytic properties.NaCl addition was found to play important role as composites synthesized without NaCl could not adsorb enrofloxacin well.Adding NaCl into the hydrothermal treatment causes sodium ions to be simultaneously intercalated into the composite structure,improving ENR adsorption greatly from 1 to 6 mg ENR/g composite.It also slows down the MX to TiO2 conversion leading to a smaller and more uniform distribution of TiO_(2) particles on the structure.MX-TiO_(2)/NaCl composites,which have sodium intercalated in their structures,showed both higher ENR adsorption and photocatalytic activity than composites without NaCl despite the latter having higher TiO2 content.Adsorbed ENR on the composites can be efficiently degraded by free radicals generated from the photoexcited TiO2 particles,leading to high photocatalytic degradation efficiency.This demonstrates the synergetic effect between adsorption and photocatalytic degradation of the synthesized compounds. 展开更多
关键词 MXene-TiO_(2)composites Ti3C2 Ion-exchange Photocatalytic degradation enrofloxacin Antibiotics removal
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典型抗菌药物在不同品种肉鸡羽毛和皮脂中的残留规律研究
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作者 陈大伟 张静 +6 位作者 刘茵茵 马丽娜 唐修君 陆俊贤 赵敏 万玉 高玉时 《安徽农业科学》 CAS 2024年第22期85-87,共3页
[目的]探索典型抗菌药物在不同品种肉鸡羽毛和皮脂中的残留规律,评价羽毛在肉鸡兽药残留监控中的意义。[方法]选择雪山鸡和AA肉鸡于出栏前21 d投喂氟苯尼考和恩诺沙星,测定停药后不同时间羽毛和皮脂中的药物残留量。[结果]停药后第1天2... [目的]探索典型抗菌药物在不同品种肉鸡羽毛和皮脂中的残留规律,评价羽毛在肉鸡兽药残留监控中的意义。[方法]选择雪山鸡和AA肉鸡于出栏前21 d投喂氟苯尼考和恩诺沙星,测定停药后不同时间羽毛和皮脂中的药物残留量。[结果]停药后第1天2个品种肉鸡羽毛中氟苯尼考及其代谢物(氟苯尼考胺)的残留总量达到最高水平,且含量相当。停药后第1天雪山鸡羽毛中恩诺沙星及其代谢物(环丙沙星)残留总量达到峰值,AA肉鸡于停药后第3天达到峰值,且雪山鸡羽毛中药物残留总量峰值远高于AA肉鸡。此后,羽毛中药物残留总量逐渐下降,停药后第14天氟苯尼考及其代谢物在AA肉鸡和雪山鸡羽毛中的残留总量分别为281.37和424.30μg/kg,恩诺沙星及其代谢物残留总量分别为16.03和26.21μg/kg。皮脂中2种药物的残留规律与羽毛中2种药物的残留规律类似,提示通过监测羽毛中的药物残留量可以间接推断可食用组织的药物残留情况。[结论]同种药物在不同品种肉鸡羽毛中的代谢规律存在差异,羽毛中药物残留量可作为监测肉鸡产品中药物残留水平的参考。 展开更多
关键词 氟苯尼考 恩诺沙星 羽毛 皮脂 残留规律
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基于造纸污泥的活性炭对恩诺沙星的吸附研究
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作者 王潇潇 陈燕 柳炜 《中国造纸》 CAS 北大核心 2024年第2期158-164,共7页
本研究首先从造纸污泥制备炭化物(CCP),再利用磷酸/硝酸混合液活化CCP,制备造纸污泥基活性炭(ACPMS)。将ACPMS用于抗生素恩诺沙星(ENF)的吸附去除。结果表明,ENF在ACPMS上的吸附过程符合朗格缪尔等温线模型和准二阶动力学模型,最大吸附... 本研究首先从造纸污泥制备炭化物(CCP),再利用磷酸/硝酸混合液活化CCP,制备造纸污泥基活性炭(ACPMS)。将ACPMS用于抗生素恩诺沙星(ENF)的吸附去除。结果表明,ENF在ACPMS上的吸附过程符合朗格缪尔等温线模型和准二阶动力学模型,最大吸附能力为46.8 mg/g,表明ACPMS可以高效吸附ENF,是一种从环境基质中去除抗生素的有效工具。 展开更多
关键词 恩诺沙星 吸附 造纸污泥 活性炭
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牛肉粉中恩诺沙星和磺胺嘧啶高准确度分析方法研究与标准物质研制 被引量:1
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作者 李瑶瑶 杨吉双 +3 位作者 李秀琴 时玲 高燕 张庆合 《化学试剂》 CAS 2024年第5期93-99,共7页
建立了基于液相色谱-同位素稀释质谱法(LC-IDMS)的牛肉粉中恩诺沙星和磺胺嘧啶残留测定的高准确度分析方法,采用同位素稀释单点校准定量,恩诺沙星和磺胺嘧啶的加标回收率分别为(99.0±0.8)%和(100.4±1.2)%,基质效应因子分别为0... 建立了基于液相色谱-同位素稀释质谱法(LC-IDMS)的牛肉粉中恩诺沙星和磺胺嘧啶残留测定的高准确度分析方法,采用同位素稀释单点校准定量,恩诺沙星和磺胺嘧啶的加标回收率分别为(99.0±0.8)%和(100.4±1.2)%,基质效应因子分别为0.9991和1.0119。采用添加法制备牛肉粉中恩诺沙星和磺胺嘧啶标准物质候选物,进行了标准物质均匀性检验、稳定性检验和定值,评定了不确定度。标准物质均匀性、稳定性良好,恩诺沙星特性量值为729 ng/g,扩展不确定度为42 ng/g(k=2),磺胺嘧啶特性量值为413 ng/g,扩展不确定度为31 ng/g(k=2)。该标准物质的研制能够应用于食品中恩诺沙星和磺胺嘧啶等相关兽药残留检测的质量控制、方法开发和验证等领域,进而保障相关检测结果的准确性、可比性。 展开更多
关键词 牛肉粉 恩诺沙星 磺胺嘧啶 同位素稀释质谱法 标准物质
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恩诺沙星及其代谢物环丙沙星在牛蛙体内的残留消除规律研究 被引量:1
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作者 董军 张思雨 +4 位作者 房迪 苏泽辉 舒锐 雷小婷 胡鲲 《淡水渔业》 CSCD 北大核心 2024年第1期66-75,共10页
为研究恩诺沙星及其代谢产物环丙沙星在牛蛙(Lithobates catesbeiana)体内药代动力学及分布,明确恩诺沙星在牛蛙体内的最大残留限量及休药期。本实验进行牛蛙单次口灌10%恩诺沙星粉混悬液(20 mg/kg·bw),在0.5、1、2、4、8、12、24... 为研究恩诺沙星及其代谢产物环丙沙星在牛蛙(Lithobates catesbeiana)体内药代动力学及分布,明确恩诺沙星在牛蛙体内的最大残留限量及休药期。本实验进行牛蛙单次口灌10%恩诺沙星粉混悬液(20 mg/kg·bw),在0.5、1、2、4、8、12、24、48、96、192、336、480 h分组织取样检测实验。研究显示:恩诺沙星和环丙沙星在牛蛙血液中的达峰时间(T_(max))相同为1 h,峰浓度(C_(max))分别为26.592μg/mL、2.256μg/mL;恩诺沙星在牛蛙肌肉、肝脏、肾脏、脑中的T_(max)均为8 h,C_(max)分别为10.21、13.84、17.52、3.95 mg/kg;环丙沙星在牛蛙肌肉、肝脏、肾脏的T_(max)分别为12、8、12 h,C_(max)分别为1.362、20.46、4.07 mg/kg,在脑中未检测出环丙沙星。结果表明:恩诺沙星在牛蛙体内吸收迅速,21.90%~40.35%恩诺沙星转化为环丙沙星,与鱼类和甲壳类生物相比,环丙沙星在牛蛙体内更易消除;恩诺沙星在牛蛙体内的最大残留限量MRL为108.22μg/kg;单剂量10%恩诺沙星粉(20 mg/kg bw)适用于治疗由于嗜水气单胞菌(MIC:0.1μg/mL)患病牛蛙;牛蛙在室温为25℃左右的饲养环境下,建议休药期不低于514.24 h(22 d)。 展开更多
关键词 恩诺沙星 牛蛙(Lithobates catesbeiana) 残留 休药期
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2种抗生素对大黄鱼肠道组织及紧密连接蛋白基因表达的影响
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作者 成好学 王庚申 +8 位作者 许叶祥 王莹 刘慧玲 徐伟 施慧 谢建军 汪玮 张东旭 许文军 《水产科学》 CAS CSCD 北大核心 2024年第4期531-540,共10页
为探究盐酸多西环素和恩诺沙星对大黄鱼肠道结构及肠道紧密连接蛋白相关基因表达的影响,在水温16.80~22.50℃下,将体质量(41.57±1.63)g的大黄鱼饲养在直径1 m、水深1.25 m的圆形塑料水槽中,投喂剂量0、0.2、1.0、2.0 g/kg的药饵5~7... 为探究盐酸多西环素和恩诺沙星对大黄鱼肠道结构及肠道紧密连接蛋白相关基因表达的影响,在水温16.80~22.50℃下,将体质量(41.57±1.63)g的大黄鱼饲养在直径1 m、水深1.25 m的圆形塑料水槽中,投喂剂量0、0.2、1.0、2.0 g/kg的药饵5~7 d,停药后1、5、15 d,观察肠道组织形态变化,测定紧密连接蛋白基因Claudin-7、ZO-1和Occludin的相对表达量。试验结果显示:大黄鱼摄食2种药物后,肠道均出现肠绒毛萎缩,组织空泡化等病变,随着给药剂量的增加和给药时间的延长,肠道组织损伤程度逐渐加深,紧密连接蛋白基因表达量持续降低;肠黏膜损伤在停药15 d后仍未恢复至对照组水平,药物剂量越高恢复程度越低;与对照组相比,2种药物高剂量组紧密连接蛋白基因表达量在停药15 d后仍显著低于对照组(P<0.05),其余组无显著差异(P>0.05)。试验结果表明,盐酸多西环素和恩诺沙星以剂量时间依赖方式损伤大黄鱼肠道组织,降低肠道通透性,药物剂量越高,恢复周期越长,在15 d后仍未完全恢复。 展开更多
关键词 盐酸多西环素 恩诺沙星 大黄鱼 肠道 组织病理 紧密连接蛋白基因
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预防刺参腐皮综合征复方中药的筛选及体内药效评价
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作者 庞明海 王春元 +6 位作者 王印庚 李成华 廖梅杰 张正 荣小军 李彬 刘妮 《水生生物学报》 CAS CSCD 北大核心 2024年第7期1130-1140,共11页
为开发防治刺参腐皮综合征的复方中药,从14种中药中筛选出对灿烂弧菌(Vibrio splendidus)有明显抑制作用的单味中药,并通过正交实验确定最佳抗菌复方FF-Z(诃子﹕五倍子﹕穿心莲﹕川芎=15 g﹕2 g﹕10 g﹕5 g)。在饲料中分别添加10、20、3... 为开发防治刺参腐皮综合征的复方中药,从14种中药中筛选出对灿烂弧菌(Vibrio splendidus)有明显抑制作用的单味中药,并通过正交实验确定最佳抗菌复方FF-Z(诃子﹕五倍子﹕穿心莲﹕川芎=15 g﹕2 g﹕10 g﹕5 g)。在饲料中分别添加10、20、30和40 g/kg的复方FF-Z作为试验组,不含复方FF-Z的饲料为对照组,并以含恩诺沙星粉20 g/kg的饲料为药物对照组,连续投喂刺参10d,继而用终浓度为10^(7) CFU/mL的灿烂弧菌进行浸浴攻毒,以观察预防保护效果。结果显示,灿烂弧菌对复方FF-Z极敏感,抑菌圈直径可达(23.10±0.62)mm,最小抑菌浓度(MIC)和最小杀菌浓度(MBC)值分别为7.81和15.63 mg/mL;与阴性对照组相比,复方FF-Z各剂量组刺参的SOD、ACP和AKP活性显著提高,且优于药物对照组。在灿烂弧菌感染后,复方FF-Z各剂量组处理的刺参发病时间比阳性对照组推迟2d,其中复方FF-Z 20 g/kg组、FF-Z 30 g/kg组和FF-Z 40 g/kg组刺参的20d发病率分别为22.08%、22.14%和14.58%,显著低于阳性对照组的84.45%。组织病理学观察结果表明,添加30 g/kg复方FF-Z的饲料能够有效减轻灿烂弧菌入侵引起的刺参组织炎症反应,从而起到保护作用。研究证明,复方FF-Z能够显著提高刺参的免疫和抗病力,对灿烂弧菌引起的腐皮综合征具有良好的预防保护效果,能延缓该病的发病进程并降低发病率。 展开更多
关键词 腐皮综合征 灿烂弧菌 中药复方 恩诺沙星 体外抑菌 刺参
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