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Follicle-stimulating Hormone(FSH) Induced Internalization of Porcine FSH Receptor in Cultured Porcine Granulosa Cells and Chinese Hamster Ovary Cells Transfected with Recombinant Porcine FSH Receptor cDNA
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作者 朱长虹 田红 +1 位作者 熊忠明 肖惠珠 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2001年第3期188-190,共3页
In order to study the fate of human follicle-stimulating hormone (FSH) when hormone binds to its receptor, a quick biochemical method that can differentiate between the surface-bound and internalized hormone was used ... In order to study the fate of human follicle-stimulating hormone (FSH) when hormone binds to its receptor, a quick biochemical method that can differentiate between the surface-bound and internalized hormone was used to determine the internalization induced by FSH in cultured both porcine granulosa cells and Chinese hamster ovary (CHO) cells expressing recombinant porcine FSH receptor. The results showed that FSH was slowly internalized, and the internalized radioactivity (acid resistant) reached a peak 10-12 h after addition of 125 I-hFSH. It was suggested that FSHR do not get internalized rapidly under physiological circumstances precisely because the appropriate sequences are absent. 展开更多
关键词 follicle-stimulating hormone INTERNALIZATION granulosa cells
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关元命门序贯针刺激活FSHR/cAMP/PKA通路促进早发性卵巢功能不全模型大鼠颗粒细胞增殖的机制研究 被引量:1
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作者 许江虹 陈跃来 +4 位作者 尹平 赵雪丹 郑慧敏 胡俊威 刘璐慜 《广州中医药大学学报》 CAS 2024年第4期965-972,共8页
【目的】观察关元命门序贯针刺方案对早发性卵巢功能不全(POI)模型大鼠的治疗作用及机制。【方法】将雌性SD大鼠分为空白组、模型组、蛋白激酶A(PKA)抑制剂(H89)+针刺组、针刺组各12只。除空白组,其他3组大鼠采用雷公藤多苷片灌胃制备PO... 【目的】观察关元命门序贯针刺方案对早发性卵巢功能不全(POI)模型大鼠的治疗作用及机制。【方法】将雌性SD大鼠分为空白组、模型组、蛋白激酶A(PKA)抑制剂(H89)+针刺组、针刺组各12只。除空白组,其他3组大鼠采用雷公藤多苷片灌胃制备POI模型。模型成功建立后,空白组和模型组每日捆绑一次;针刺组大鼠在动情间期取关元穴针刺,在动情前期取命门穴针刺;H89+针刺组按照针刺组针刺方案干预,在每次针刺前30 min内腹腔注射H89。连续干预20 d。各组大鼠分别在干预后第1个动情间期和动情前期取材。酶联免疫吸附分析(ELISA)检测动情间期促卵泡激素(FSH)、雌二醇(E2)水平,Western Blot法检测动情间期促卵泡激素受体(FSHR)、芳香化酶P450(P450arom)蛋白表达,细胞计数试剂盒8(CCK-8)法检测动情间期和动情前期颗粒细胞活性,免疫组织化学法检测动情前期增殖细胞核抗原(PCNA)蛋白表达水平。【结果】(1)与空白组比较,模型组和H89+针刺组血清FSH水平显著升高(P<0.01),E2水平显著降低(P<0.001);H89+针刺组FSH水平与模型组无差异(P>0.05),E2水平低于模型组(P<0.05);针刺组FSH水平低于模型组和H89+针刺组(P<0.05),与空白组无差异(P>0.05),E2水平显著高于模型组和H89+针刺组(P<0.01),仍低于空白组(P<0.05)。(2)模型组和H89+针刺组FSHR、P450arom蛋白表达均低于空白组(P<0.01);H89+针刺组FSHR蛋白表达水平与模型组无差异(P>0.05),P450arom蛋白表达水平低于模型组(P<0.05);针刺组FSHR、P450arom蛋白表达水平均高于模型组和H89+针刺组(P<0.05),但仍低于空白组(P<0.05)。(3)模型组和H89+针刺组GCs活性和PCNA平均光密度值均低于空白组(P<0.05);H89+针刺组GCs活性和PCNA平均光密度值均低于模型组(P<0.05);针刺组的GCs活性和PCNA平均光密度值显著高于模型组和H89+针刺组(P<0.05或P<0.01)。【结论】关元命门序贯针刺方案可通过上调促卵泡激素受体(FSHR)/环磷酸腺苷(cAMP)/蛋白激酶A(PKA)通路FSHR、P450arom蛋白的表达,调控性激素水平,提高GCs活性和促进GCs细胞增殖,从而改善POI。 展开更多
关键词 序贯针刺 早发性卵巢功能不全 关元穴 命门穴 促卵泡激素受体(fshR) 芳香化酶P450(P450arom) 大鼠
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血清HE4、LH、FSH对宫颈癌和宫颈上皮内瘤变的鉴别诊断价值
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作者 王衡 高湛 +1 位作者 王蕊 冉繁华 《实用癌症杂志》 2024年第11期1900-1903,1908,共5页
目的探究血清人附睾蛋白4(HE4)、促黄体生成素(LH)、卵泡刺激素(FSH)对宫颈癌和宫颈上皮内瘤变(CIN)的鉴别诊断价值。方法选择89例接受宫颈癌筛查的宫颈病变患者,经病理学或细胞学明确诊断宫颈癌51例、CIN 38例,检测所有患者血清HE4、LH... 目的探究血清人附睾蛋白4(HE4)、促黄体生成素(LH)、卵泡刺激素(FSH)对宫颈癌和宫颈上皮内瘤变(CIN)的鉴别诊断价值。方法选择89例接受宫颈癌筛查的宫颈病变患者,经病理学或细胞学明确诊断宫颈癌51例、CIN 38例,检测所有患者血清HE4、LH、FSH水平,分析宫颈癌患者与CIN患者血清检测指标水平差异,采用受试者特征工作曲线(ROC曲线)、二元logistics回归分析法评价HE4、LH、FSH在疾病鉴别中的价值。结果宫颈癌患者血清HE4、LH、FSH水平均显著高于CIN患者(P<0.05);宫颈病变患者血清HE4与LH、FSH水平呈正相关(γ=0.357、0.392,P<0.05)。ROC曲线分析显示,血清HE4、LH、FSH诊断宫颈癌的敏感性均较低,三者联合诊断效能较单一指标更高,联合诊断曲线下面积为0.859,诊断敏感度88.24%、特异度71.05%;logistics回归分析显示,HE4>62.84 pmol/ml是宫颈癌发病的影响因素(P<0.05)。结论宫颈癌患者血清HE4、LH、FSH水平异常升高,且指标间呈正相关,三者联合用于鉴别诊断宫颈癌和CIN具有一定临床价值。 展开更多
关键词 宫颈癌 宫颈上皮内瘤变 人附睾蛋白 促黄体生成素 卵泡刺激素 价值
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FSHR基因多态性、BMI及性激素与多囊卵巢综合征发病风险的相关性分析 被引量:1
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作者 昝志芳 土增荣 +3 位作者 王淇蓉 段毓 刘建兵 李莉 《解放军医学杂志》 CAS CSCD 北大核心 2024年第1期50-56,共7页
目的探讨体重指数(BMI)、性激素及卵泡刺激素受体(FSHR)基因rs2268361和rs2349415位点的单核苷酸多态性(SNP)与多囊卵巢综合征(PCOS)发病风险的相关性。方法收集2021年3-8月山西医科大学第一医院生殖医学科门诊就诊的213例PCOS患者(PCOS... 目的探讨体重指数(BMI)、性激素及卵泡刺激素受体(FSHR)基因rs2268361和rs2349415位点的单核苷酸多态性(SNP)与多囊卵巢综合征(PCOS)发病风险的相关性。方法收集2021年3-8月山西医科大学第一医院生殖医学科门诊就诊的213例PCOS患者(PCOS组)与207名健康对照者(对照组)的外周血,随机收集PCOS组与对照组各32例卵泡液。计算PCOS组与对照组的BMI;采用免疫化学发光法检测两组外周血卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E_(2))、睾酮(T)、孕酮(P)和泌乳素(PRL)水平;采用聚合酶链式反应(PCR)和高分辨熔解曲线(HRM)分析两组FSHR基因rs2268361和rs2349415位点多态性;采用实时定量PCR检测两组外周血及卵巢颗粒细胞FSHR mRNA表达水平。结果LH与LH/FSH呈明显正相关(r=0.88,P<0.05);PCOS组BMI、E2、LH、LH/FSH、T水平明显高于对照组(P<0.05),FSH水平明显低于对照组(P<0.001);HRM分析显示,PCOS组rs2349415位点CC、CT、TT基因型频率分别为55.9%、34.3%和9.8%,对照组分别为68.6%、23.2%和8.2%,PCOS组C、T等位基因频率分别为73.0%、27.0%,对照组分别为80.2%、19.8%,两组基因型频率及等位基因频率比较差异均有统计学意义(P<0.05);PCOS组卵巢颗粒细胞中FSHR mRNA表达水平明显高于对照组(P=0.004),其中rs2349415 TT基因型FSHR mRNA表达水平高于CC(P=0.002)和CT基因型(P=0.035)。结论高水平的BMI、LH、E2及rs2349415位点T等位基因可增加PCOS的发病风险。 展开更多
关键词 多囊卵巢综合征 卵泡刺激素受体 单核苷酸多态性 性激素
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Maturation, proliferation and apoptosis of seminal tubule cells at puberty after administration of estradiol, follicle stimulating hormone or both 被引量:2
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作者 Renata Walczak-Jedrzejowska Jolanta Slowikowska-Hilczer Katarzyna Marchlewska Krzysztof Kula 《Asian Journal of Andrology》 SCIE CAS CSCD 2008年第4期585-592,共8页
Aim: To assess proliferative and apoptotic potential of the seminiferous epithelium cells in relation to Sertoli cell maturation in newborn rats under the influence of estradiol, follicle stimulating hormone (FSH) ... Aim: To assess proliferative and apoptotic potential of the seminiferous epithelium cells in relation to Sertoli cell maturation in newborn rats under the influence of estradiol, follicle stimulating hormone (FSH) or both agents given together. Methods: From postnatal day (PND) 5 to 15 male rats were daily injected with 12.5 μg of 1713-estradiol benzoate (EB) or 7.5 IU of human purified FSH (hFSH) or EB + hFSH or solvents (control). On postnatal day 16, autopsy was performed. Sertoli cell maturation/function was assessed by morphometry. Proliferation of the seminiferous epithelium cells was quantitatively evaluated using immunohistochemical labeling against proliferating cell nuclear antigen and apoptosis using the TUN-EL method. Results: Although EB inhibited Sertoli cell maturation and hFSH was not effective, a pronounced acceleration of Sertoli cell maturation occurred after EB + hFSH. Whereas hFSH stimulated Sertoli cell proliferation, EB or EB + hFSH inhibited Sertoli cell proliferation. All treatments significantly stimulated germ cell proliferation. Apoptosis of Sertoli cells increased 9-fold and germ cells 2-fold after EB, and was not affected by hFSH but was inhibited after EB + hFSH. Conclusion: At puberty, estradiol inhibits Sertoli cell maturation, increases Sertoli and germ cell apoptosis but stimulates germ cell proliferation. Estradiol in synergism with FSH, but neither of the hormones alone, accelerates Sertoli cell maturation associated with an increase in germ cell survival. Estradiol and FSH cooperate to induce seminal tubule maturation and trigger first spermatogenesis. 展开更多
关键词 ESTRADIOL follicle stimulating hormone germ cells Sertoli cells PROLIFERATION apoptosis
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Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells 被引量:2
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作者 Yuebing Qiao Xiuyan Ma Huixian Cui 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第6期656-658,共3页
BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect... BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect of different dosages of leptin on luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion from in vitro cultured rat anterior pituitary cells. DESIGN: Contrast study based on cells. SETTING: This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS: Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS: Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10^-12, 1×10^-11, 1×10^-9, 1×10^-7, and 1×10^-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES: Contents of LH and FSH were detected by radioimmunology. RESULTS: Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10^-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations (P 〈 0.01). LH release in the leptin (1×10^-12, 1×10^-11, 1×10^-7, and 1×10^-6 mol/L) groups was significantly higher than that in the control group (P 〈 0.01). FSH content in the leptin (1×10^-11, 1×10^-9, and 1×10^-7 mol/L) groups was significantly higher than that in the control group (P 〈 0.01). CONCLUSION: Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner. 展开更多
关键词 LEPTIN anterior pituitary luteinizing hormone follicle stimulating hormone
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Association between Two Polymorphisms of Follicle Stimulating Hormone Receptor Gene and Susceptibility to Polycystic Ovary Syndrome: a Meta-analysis 被引量:3
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作者 Lei Qiu Jing Liu Qi-ming Hei 《Chinese Medical Sciences Journal》 CAS CSCD 2015年第1期44-50,共7页
Objective To investigate the association between two polymorphisms of follicle stimulating hormone receptor (FSHR) gene and polycystic ovary syndrome (PCOS) susceptibility. Methods Case-control studies on relatio... Objective To investigate the association between two polymorphisms of follicle stimulating hormone receptor (FSHR) gene and polycystic ovary syndrome (PCOS) susceptibility. Methods Case-control studies on relationship of Thr307Ala and Asn680Ser polymorphisms in FSHR gene and PCOS susceptibility were searched from PubMed, ISI web of knowledge, EBSCO, and China National Knowledge Infrastructure (CNKI) databases up to March 21, 2013. The pooled odds ratio (OR) and 95% confidence interval (CO were calculated using fixed- or random-effect model based on heterogeneity test in 5 genotype models analyses. Results A total of 11 studies were included in the Meta-analysis. The random-effect analysis showed Asn680Ser was significantly associated with the reduced susceptibility to PCOS with dominant model (Asn/Asn+Asn/Ser vs. Ser/Ser, OR=0.83, 95% CI: 0.69-1.00), recessive model (Asn/Asn vs. Asn/Ser+ Ser/Ser, OR=0.84, 95% CI: 0.72-0.98), homozygote comparison (Ash/Ash vs. Ser/Ser, 0R=0.79, 95% CI: 0.63-0.98), and the allele contrast (Asn vs. Ser, OR=0.87, 95% CI: 0.79-0.97) respectively(P=0.02, I2=56.0%), being protective factors for PCOS. However, no significant associations were found between Thr307Ala and PCOS. Conclusion There might be a significant association between Asn680Ser polymorphism and PCOS. 展开更多
关键词 follicle stimulating hormone receptor polycystic ovary syndrome META-ANALYSIS single-nucleotide polymorphism
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Urinary follicle stimulating hormone can be used as a biomarker to assess male reproductive function 被引量:1
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作者 Xin-RuWANG JamesWOverstreet +5 位作者 HeatherTodd QingQIU Jiang-HuaYANG Shu-YiWANG Xi-PingXU BillLLasley 《Asian Journal of Andrology》 SCIE CAS CSCD 1999年第1期67-72,共6页
Aim: To develop an algorithm for use in population-based studies to assess testicular function by measurements of totalurinary follicle stimulating hormone (FSH). Methods: Total concentrations of urinary FSH were meas... Aim: To develop an algorithm for use in population-based studies to assess testicular function by measurements of totalurinary follicle stimulating hormone (FSH). Methods: Total concentrations of urinary FSH were measured in a groupof 44 men at the University of California, Davis (UCD) and were compared to FSH measurements in serum. On thebasis of these and other published data, a urinary FSH value of >2 ng/mg creatinine (Cr) was selected as the cutoffpoint to identify men with elevated serum FSH ( > 12 IU/L) or low sperm counts ( < 20 million/mL). Results: Thesensitivity and specificity of this algorithm for detecting elevated serum FSH in a group of 58 agricultural workers in thePeople's Republic of China were 100 % and 50 %, respectively. The sensitivity and specificity of this algorithm fordetecting low sperm counts in a population of 105 infertility patients at UCD were 58 % and 76 %, respectively.Conclusion: This test may have particular value in identifying populations with no evidence of testicular toxicity, andin which labor-intensive semen studies may not be feasible. 展开更多
关键词 follicle stimulating hormone sperm count REPRODUCTION TESTIS SPERMATOZOA URINE URINALYSIS
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不同繁殖力新疆山羊发情周期血清FSH和LH浓度变化规律研究
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作者 全凯丽 刘建新 +2 位作者 吕雪峰 邢巍婷 郑文新 《黑龙江动物繁殖》 2024年第5期1-6,共6页
为了探究不同繁殖力母羊发情周期血清中促卵泡激素(FSH)和促黄体激素(LH)分泌变化规律,试验选择新疆山羊高繁殖力母羊17只和低繁殖力母羊14只,采用酶联免疫分析法测定母羊发情周期血清中FSH和LH浓度。结果表明:高、低繁殖力母羊发情周期... 为了探究不同繁殖力母羊发情周期血清中促卵泡激素(FSH)和促黄体激素(LH)分泌变化规律,试验选择新疆山羊高繁殖力母羊17只和低繁殖力母羊14只,采用酶联免疫分析法测定母羊发情周期血清中FSH和LH浓度。结果表明:高、低繁殖力母羊发情周期中FSH和LH浓度均呈脉冲波动式分泌,高繁殖力母羊血清中FSH浓度在发情周期第1,5,13,21天出现4个波峰,LH浓度在发情周期第2,5,11,21天出现4次波峰;低繁殖力母羊血清中FSH浓度在发情周期第1,5,21天出现3个波峰,LH浓度在发情周期第5,13,21天出现3次波峰。在发情期和发情前期母羊血清中FSH和LH浓度均高于发情后期和间情期,高、低繁殖力母羊血清中FSH和LH浓度均在发情前期(>17~21 d)开始升高,在发情期(0~48 h)当天达到峰值之后呈波动式下降趋势,且高繁殖力母羊发情周期4个阶段血清中FSH和LH浓度均高于低繁殖力母羊。高、低繁殖力母羊血清中FSH和LH基础浓度均在发情期最高,间情期最低。说明不同繁殖力新疆山羊母羊发情周期中FSH和LH分泌变化规律一致,高繁殖力母羊在整个发情周期中FSH和LH浓度高于低繁殖力母羊且波峰数量多于低繁殖力母羊。 展开更多
关键词 新疆山羊 生殖激素 促卵泡激素(fsh) 促黄体激素(LH) 繁殖力
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Fluoride Exposure,Follicle Stimulating Hormone Receptor Gene Polymorphism and Hypothalamus-pituitary-ovarian Axis Hormones in Chinese Women 被引量:2
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作者 ZHAO Ming Xu ZHOU Guo Yu +7 位作者 ZHU Jing Yuan GONG Biao HOU Jia Xiang ZHOU Tong DUAN Li Ju DING Zhong CUI Liu Xin BA Yue 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第9期696-700,共5页
The effects of fluoride exposure on thefunctions of reproductive and endocrine systemshave attracted widespread attention in academiccircle nowadays. However, it is unclear whether thegene-environment interaction may ... The effects of fluoride exposure on thefunctions of reproductive and endocrine systemshave attracted widespread attention in academiccircle nowadays. However, it is unclear whether thegene-environment interaction may modify thesecretion and activity of hypothalamus-pituitary-ovarian (HPO) axis hormones. Thus, the aim of thisstudy was to explore the influence of fluorideexposure and follicle stimulating hormone receptor(FSHR) gene polymorphism on reproductivehormones in Chinese women. A cross sectionalstudy was conducted in seven villages of HenanProvince, China during 2010-2011. A total of 679women aged 18-48 years were recruited throughcluster sampling and divided into three groups, i.e.endemic fluorosis group (EFG), defluoridationproject group (DFPG), and control group (CG) basedon the local fluoride concentration in drinkingwater. The serum levels of gonadotropin releasinghormone (GnRH), follicle stimulating hormone(FSH), luteinizing hormone (LH), and estradiol (E2)were determined respectively and the FSHRpolymorphism was detected by real time PCR assay.The results provided the preliminary evidenceindicating the gene-environment interaction onHPO axishormones in women. 展开更多
关键词 fshR Fluoride Exposure follicle stimulating hormone Receptor Gene Polymorphism and Hypothalamus-pituitary-ovarian Axis hormones in Chinese Women LH gene
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The preparation and application of N-terminal 57 amino acid protein of the follicle-stimulating hormone receptor as a candidate male contraceptive vaccine 被引量:3
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作者 Cheng Xu Ying-Chun Li +7 位作者 Hua Yang Yan Long Min-Jian Chen Yu-Feng Qin Yan-Kai Xia Ling Song Ai-Hua Gu Xin-Ru Wang 《Asian Journal of Andrology》 SCIE CAS CSCD 2014年第4期623-630,共8页
Follicle-stimulating hormone receptor (FSHR), which is expressed only on Sertoli cells and plays a key role in spermatogenesis, has been paid attention for its potential in male contraception vaccine research and de... Follicle-stimulating hormone receptor (FSHR), which is expressed only on Sertoli cells and plays a key role in spermatogenesis, has been paid attention for its potential in male contraception vaccine research and development. This study introduces a method for the preparation and purification of human FSHR 57-amino acid protein (FSHR-57aa) as well as determination of its immunogenicity and antifertility effect. A recombinant pET-28a(+)-FSHR-57aa plasmid was constructed and expressed in Escherichia coil strain BL21 StarTM (DE3) and the FSHR-57aa protein was separated and collected by cutting the gel and recovering activity by efficient refolding dialysis. The protein was identified by Western blot and high-performance liquid chromatography analysis with a band of nearly 7 kDa and a purity of 97.4%. Male monkeys were immunized with rhFSHR-57aa protein and a gradual rising of specific serum IgG antibody was found which reached a plateau on day 112 (16 weeks) after the first immunization. After mating of one male with three female monkeys, the pregnancy rate of those mated with males immunized against FSHR-57aa was significantly decreased while the serum hormone levels of testosterone and estradiol were not disturbed in the control or the FSHR-57aa groups. By evaluating pathological changes in testicular histology, we found that the blood-testis barrier remained intact, in spite of some small damage to Sertoli cells. In conclusion, our study demonstrates that the rhFSHR-57aa protein might be a feasible male contraceptive which could affect sperm production without disturbing hormone levels. 展开更多
关键词 follicle-stimulating hormone receptor prokaryotic recombinant expression male contraception VACCINE
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Follicle-stimulating hormone autoantibody is involved in idiopathic spermatogenic dysfunction 被引量:2
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作者 Bing Yao Jian Wang +2 位作者 Wei Liang Ying-Xia Cui Yi-Feng Ge 《Asian Journal of Andrology》 SCIE CAS CSCD 2008年第6期915-921,共7页
Aim: To detect the anti-follicle-stimulating hormone (FSH) antibody in idiopathic infertile patients and fertile subjects in order to determine the role of this antibody in patients with spermatogenic dysfunction. ... Aim: To detect the anti-follicle-stimulating hormone (FSH) antibody in idiopathic infertile patients and fertile subjects in order to determine the role of this antibody in patients with spermatogenic dysfunction. Methods: The anti-FSH antibody in serum was detected by an enzyme-linked immunosorbent assay (ELISA). The functional and structural integrity of the sperm membrane was evaluated with hypo-osmotic swelling (HOS) test and the ultrastructure of the spermatozoa was investigated by transmission electron microscopy (TEM). Results: The extent of positive FSH antibody in the patients with oligozoospermia and/or asthenozoospermia was significantly higher than that in the fertile subjects and infertile patients with normal sperm concentration and motility, but it was significantly lower than that in the patients with azoospermia. The extent of anti-FSH antibody in the patients with azoospermia was significantly greater than that in patients with oligospermia and/or asthenospermia, infertile people with normal sperm density and motility and fertile people. The hypo-osmotic swelling test showed that the percentage of HOS-positive spermatozoa (swollen) was 45.1% ±3.5% in the FSH antibody-positive group and 59.1% ± 6.2% in the FSH antibody-negative control group. The percentage of functional membrane damage to spermatozoa was significantly higher in the anti- FSH antibody-positive group than in the control group. TEM showed that the outer acrosomal membrane was located far from the nucleus, and detachment of the acrosome was found in the FSH autoantibody-positive group. Conclusion: These data suggest that the presence of anti-FSH antibody is strongly correlated with the sperm quantity and quality in idiopathic male infertility. Anti-FSH antibody may be an important factor causing spermatogenic dysfunction and infertility. 展开更多
关键词 follicle-stimulating hormone ANTIBODY REPRODUCTION spermatogenic dysfunction
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来曲唑、FSH促排卵方案及HRT方案在PCOS患者冻融单囊胚胎移植内膜准备中的应用
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作者 何雪梅 李萍 《海南医学》 CAS 2024年第14期2017-2020,共4页
目的探讨来曲唑、卵泡刺激素(FSH)促排卵方案及激素替代治疗(HRT)方案在多囊卵巢综合征(PCOS)患者冻融单囊胚胎移植内膜准备中的应用效果。方法选择2021年1月至2022年12月厦门市妇幼保健院206例接受冻融单囊胚移植的PCOS患者作为研究对... 目的探讨来曲唑、卵泡刺激素(FSH)促排卵方案及激素替代治疗(HRT)方案在多囊卵巢综合征(PCOS)患者冻融单囊胚胎移植内膜准备中的应用效果。方法选择2021年1月至2022年12月厦门市妇幼保健院206例接受冻融单囊胚移植的PCOS患者作为研究对象,按随机数表法分为A组69例、B组69例和C组68例。三组患者分别采用不同内膜准备方案,其中A组使用来曲唑方案,B组使用FSH促排卵方案,C组使用HRT方案。比较三组患者的治疗情况、妊娠情况及母婴结局。结果三组患者的胚胎移植个数比较差异无统计学意义(P>0.05);三组患者的生化妊娠、临床妊娠、异位妊娠比较差异均无统计学意义(P>0.05),而A组患者的持续妊娠、活产率分别为60.87%、57.97%,B组分别为59.42%、55.07%,均高于C组的41.18%、36.76%,差异均有统计学意义(P<0.05),但A组和B组患者的持续妊娠、活产率比较差异无统计学意义(P>0.05);三组患者的妊娠期糖尿病、妊娠期高血压、前置胎盘、剖宫产、产后出血发生率及分娩孕周、新生儿体质量和早产比较差异均无统计学意义(P>0.05)。结论与HRT方案相比,使用来曲唑、FSH促排卵方案进行内膜准备有助于提高PCOS患者冻融单囊胚胎移植后的持续妊娠率及活产率。 展开更多
关键词 多囊卵巢综合征 胚胎移植 来曲唑 卵泡刺激素 激素替代治疗 母婴结局
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Cloning and Transcriptional Activity of Follicle-Stimulating Hormone Receptor Promoter in the Jintang Black Goat 被引量:1
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作者 LIANG Tian-yu LI Jian CAO Ye 《Animal Husbandry and Feed Science》 CAS 2010年第2期1-4,共4页
[ Objective] To clone follicle-stimulating hormone receptor (FSHR) promoter in the Jintang black goat, study its transcriptional activity, and provide a basis for alternative splicing of FSHR gene. [Method] The tota... [ Objective] To clone follicle-stimulating hormone receptor (FSHR) promoter in the Jintang black goat, study its transcriptional activity, and provide a basis for alternative splicing of FSHR gene. [Method] The total DNA were extracted from the womb of Jintang black goat, and one pair of primers were designed for amplification of FSHR promoter fragments, then the sequences and homology were analyzed. The FSHR promoter fragment was inserted into the pcFSHRB1 expression vector to substitute the CMV promoter and construct the pcFSHRB2 expression vector. The pcFSHRB1 and pcFSHRB2 expression vectors were transformed into HEK293 cells, respectively. Then these cells were collected after 24 and 48 h treatment with 2 mlU/ml follicle-stimulating hormone (FSH), and the cAMP levels were detected. [Result] The FSHR promoter sequence of Jin- tang black goat had 34.2% homology to that of chicken and 41.6% to that of rat, respectively. The transcription initial site of FSHR was at -576 bp and its upstream sequences contained two TATA-boxes, four CAAT-boxes, one E-box and one Wl-box. After treating for 24 and 48 h, the cAMP levels of pcFSHRB2 were respectively 299.581 3 and 125.528 1 pmol/L; and that of pcFSHRB1 were respectively 120.057 1 and 109.940 7 pmoVL. [Conclusion] The FSHR promoter of Jintang black goat is a typical type 2 eukaryotic promoter, and it is also a strong promoter. 展开更多
关键词 Jintang black goat follicle-stimulating hormone receptor promoter Expression vector Cyclic adenosine monoph^osphate
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Evaluation of steroidogenic capacity after follicle stimulating hormone stimulation in bovine granulosa cells of Revalor 200~ implanted heifers
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作者 ANDrea D Stapp Craig A Gifford +1 位作者 Dennis M Hallford Jennifer A Hernandez Gifford 《Journal of Animal Science and Biotechnology》 SCIE CAS 2014年第3期280-285,共6页
Background: Heifers not used as breeding stock are often implanted with steroids to increase growth efficiency thereby altering hormone profiles and potentially changing the environment in which ovarian follicles dev... Background: Heifers not used as breeding stock are often implanted with steroids to increase growth efficiency thereby altering hormone profiles and potentially changing the environment in which ovarian follicles develop. Because bovine granulosa cell culture is a commonly used technique and often bovine ovaries are collected from abattoirs with no record of implant status, the objective of this study was to determine if the presence of an implant during bovine granulosa cell development impacts follicle stimulating hormone-regulated steroidogenic enzyme expression. Paired ovaries were collected from 16 feedlot heifers subjected to 1 of 3 treatments: non-implanted (n = 5), Revalor 200 for 28 d (n = 5), or Revalor 200 for 84 d (n = 6). Small follicle (1 to 5 mm) granulosa cells were isolated from each pair and incubated with phosphate buffered saline (n = 16) or 100 ng/mL follicle stimulating hormone (n = 16) for 24 h. Results: Granulosa cells of implanted heifers treated with follicle stimulating hormone produced medium concentrations of progesterone similar (P = 0.22) to non-implanted heifers, while medium estradiol concentrations were increased (P 〈 0.10) at 28 and 84 d compared to non-implanted heifers indicating efficacy of treatment. Additionally, real-time PCR analysis in response to follicle stimulating hormone treatment demonstrated a decrease in steroidogenic acute regulatory protein (P = 0.05) mRNA expression in heifers implanted for 84 d and an increase in P450 side chain cleavage mRNA in granulosa cells of heifers implanted for 28 (P 〈 0.10) or 84 d (P 〈 0.05) compared to non-implanted females. However, no difference in expression of 3-beta-hydroxysteroid dehydrogenase (P= 0.57) and aromatase (P = 0.23) were demonstrated in implanted or non-implanted heifers. Conclusions: These results indicate follicles which develop in the presence of high concentrations of androgenic and estrogenic steroids via an implant tend to demonstrate an altered capacity to respond to follicle stimulating hormone stimulation. Thus, efforts should be made to avoid the use of implanted heifers to study steroidogenesis in small follicle granulosa cell culture systems. 展开更多
关键词 BOVINE follicle stimulating hormone Granulosa cells IMPLANT Steroidogenesis
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基础黄体生成素和不同LH/FSH比值水平对PCOS患者辅助生殖妊娠结局的影响
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作者 叶子 王菁 +1 位作者 刘嘉茵 马翔 《实用妇产科杂志》 CAS CSCD 北大核心 2024年第3期213-218,共6页
目的:探究基础黄体生成素(bLH)及不同黄体生成素/卵泡刺激素(LH/FSH)比值水平对多囊卵巢综合征(PCOS)患者体外受精或卵胞浆内单精子注射(IVF/ICSI)妊娠结局的影响。方法:选择2013年7月至2020年1月在南京医科大学第一附属医院生殖医学科... 目的:探究基础黄体生成素(bLH)及不同黄体生成素/卵泡刺激素(LH/FSH)比值水平对多囊卵巢综合征(PCOS)患者体外受精或卵胞浆内单精子注射(IVF/ICSI)妊娠结局的影响。方法:选择2013年7月至2020年1月在南京医科大学第一附属医院生殖医学科首次行促性腺激素释放激素(GnRH)拮抗剂灵活方案促排卵,接受IVF/ICSI治疗的424例PCOS患者的临床资料进行回顾性分析。按照bLH水平不同分为正常bLH组(LH≤10 U/L,316例)和高bLH组(LH>10 U/L,108例);同时根据LH/FSH比值水平不同分为低比值组(LH/FSH≤1,227例)、中比值组(1<LH/FSH<2,142例)、高比值组(LH/FSH≥2,55例)。分析比较各组患者一般情况、促排卵情况及妊娠结局的差异,并采用二元Logistic回归分析影响活产的相关因素。结果:①高bLH组基础FSH和抗苗勒管激素(AMH)较正常bLH组高,差异有统计学意义(P<0.05)。在促排卵情况方面,高bLH组使用拮抗剂总天数长于正常组,但获卵数和绒促性素(HCG)日子宫内膜厚度小于正常bLH组,差异有统计学意义(P<0.05)。两组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。②不同LH/FSH比值组患者比较,高比值组AMH和窦卵泡数(AFC)高于其他两组,差异有统计学意义(P<0.05)。在促排卵情况方面,高比值组获卵数、移植胚胎数、HCG日子宫内膜厚度均低于其他两组,差异有统计学意义(P<0.05)。3组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。③二元Logistic回归分析显示活产与bLH、LH/FSH比值无关(P>0.05),与移植胚胎数有关(P<0.05)。结论:bLH水平和LH/FSH值升高可能会影响PCOS患者促排卵结果(如获卵数和HCG日子宫内膜厚度等),但与辅助生殖的活产率未见显著相关性。 展开更多
关键词 黄体生成素 卵泡刺激素 多囊卵巢综合征 辅助生殖 妊娠结局
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Stability of Human Follicle-Stimulating Hormone Receptor mRNA in Stably Transfected Cells
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作者 朱长虹 田红 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2001年第1期8-12,共5页
In order to assess the impact of mRNA degradation on steady state levels of follicle stimulating hormone receptor (FSHR) mRNA and on regulation of FSHR gene expression, the stability and half life of FSHR mRNA were ... In order to assess the impact of mRNA degradation on steady state levels of follicle stimulating hormone receptor (FSHR) mRNA and on regulation of FSHR gene expression, the stability and half life of FSHR mRNA were determined in transfected cells expressing recombinant FSHR. Time dependent changes in FSHR mRNA content were determined by nuclease protection solution hybridization assay (NPA) or by qualitative reverse transcription competitive polymerase chain reaction (RT PCR) in cultured hFSHR YI cells, cell lines stably transfected with a human FSHR cDNA. FSHR mRNA content remained constant during 8 h control incubations of hFSHR Y1 cells (NPA, 2.9±0.3 μg/mg RNA; RT PCR, 2.7±0.3 μg/mg RNA). Actinomycin D (ActD, 5 μg/ml) inhibited mRNA synthesis, as assessed by incorporation of uridine into total RNA, by 90 % within 1 h in hFSHR Y1 cells. No effect of ActD on cellular morphology or viability was observed. ActD caused a time dependent decrease in FSHR mRNA content in hFSHR Y1 cell lines with a lag time of 1 h. There were no significant differences in the rate of FSHR mRNA degradation between the two methods of mRNA quantification. The half life of hFSHR mRNA was 3.6±0.2 h by NPA and 3.1±0.1 h by RT PCR. The results indicated that degradation of mRNA was an important process in maintenance of steady state expression of the FSHR gene in cells stably expressing recombinant receptor. 展开更多
关键词 GONADOTROPIN granulosa cell FOLLICULOGENESIS follicle stimulating hormone mRNA
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Quantification of Porcine Follicle-stimulating Hormone Receptor Messenger Ribonucleic Acid by Reverse Transcription competitive Polymerase Chain Reaction
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作者 朱长虹 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第3期177-182,共6页
An easy and reliable method was developed for construction and quantification of competitive templates, which shared the same sequence as the amplified target DNA except for a 20 bp insertion in the middle by recombi... An easy and reliable method was developed for construction and quantification of competitive templates, which shared the same sequence as the amplified target DNA except for a 20 bp insertion in the middle by recombinant polymerase chain reaction (PCR). Among the advantages of competitive PCR is that any predictable or unpredictable variable that affects amplification has the same effect on both target and competitor species and that the final ratio of amplified products reflects exactly the initial targets. The utilization of a thermostable reverse transcriptase in the RT step was proposed to overcome the problem of the efficiency of target cDNA synthesis. In addition, to obtain reliable measurements, it was recommended to perform four PCR with amounts of competitive template flanking the concentration of the target mRNA. 展开更多
关键词 follicle stimulating hormone receptor MRNA reverse transcription competitive polymerase chain reaction
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醋酸亮丙瑞林联合腹腔镜子宫肌瘤剔除术对子宫肌瘤FSH、E_(2)及LH水平的影响
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作者 许章山 《中国现代药物应用》 2024年第21期113-116,共4页
目的对子宫肌瘤患者实施醋酸亮瑞林、腹腔镜子宫肌瘤剔除术(LSM)联合方案,观察该联合方案对卵泡刺激素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)水平的影响。方法选取100例子宫肌瘤患者,根据治疗方案的个体差异性分为对照组(LSM治疗)、... 目的对子宫肌瘤患者实施醋酸亮瑞林、腹腔镜子宫肌瘤剔除术(LSM)联合方案,观察该联合方案对卵泡刺激素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)水平的影响。方法选取100例子宫肌瘤患者,根据治疗方案的个体差异性分为对照组(LSM治疗)、观察组(醋酸亮丙瑞林+LSM治疗),各50例。比较两组血清性激素水平、术后月经指标及围术期相关治疗指标。结果治疗前,对照组血清FSH、E_(2)、LH水平分别为(27.57±3.47)U/L、(275.39±33.48)pmol/L、(20.91±2.51)U/L,观察组分别为(27.61±3.36)U/L、(276.82±33.51)pmol/L、(20.85±2.49)U/L;治疗后,对照组血清FSH、E_(2)、LH水平分别为(18.17±2.84)U/L、(216.17±26.57)pmol/L、(17.49±1.85)U/L,观察组分别为(13.05±2.29)U/L、(139.84±18.79)pmol/L、(11.67±1.36)U/L。与治疗前相比,两组血清FSH、E_(2)、LH水平均明显下降,组内差异有统计学意义(P<0.05);观察组治疗后血清FSH、E_(2)、LH水平均低于对照组,组间差异有统计学意义(P<0.05)。观察组术后月经周期时间(29.56±1.32)d短于对照组的(33.13±2.29)d,月经量(41.33±4.32)ml少于对照组的(55.45±6.69)ml,组间差异有统计学意义(P<0.05)。观察组术中出血量、盆腔引流量少于对照组,下床活动时间、住院时间短于对照组,疾病复发率低于对照组,组间差异有统计学意义(P<0.05)。结论醋酸亮丙瑞林联合LSM运用于子宫肌瘤临床治疗过程中,可明显降低患者FSE、E_(2)、LH水平,减少术中出血量与盆腔引流量,加快术后身体恢复,降低疾病复发率,值得推荐。 展开更多
关键词 子宫肌瘤 醋酸亮丙瑞林 腹腔镜子宫肌瘤剔除术 卵泡刺激素 雌二醇 促黄体生成素
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Effect of Follicle-Stimulating Hormone Dosage, Seasons and Treatment Frequency on Super-Ovulation in Goats
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作者 YUAN Yu-guo AN Li-you YU Bao-li YANG Ting-jia CHENG Yong 《Animal Husbandry and Feed Science》 CAS 2011年第3期20-22,共3页
[ Objective] To explore the effects of FSH ( Follicle-stimulating hormone), seasons and treatment frequency on super-ovulation in donor goats and to provide necessary data for research about embryo transfer and embr... [ Objective] To explore the effects of FSH ( Follicle-stimulating hormone), seasons and treatment frequency on super-ovulation in donor goats and to provide necessary data for research about embryo transfer and embryo biotechnology in Yangzhou area. [ Method] Two FSH dosages (240 and 300 I U), two seasons (April- June and October- December), and two treatment frequencies (one or two times) were used to induce super-ovulation in goats. [ Result] Both average ovulation point and number of transferable embryos were significantly different between the goats given 240 IU FSH and those given 300 lU FSH (average ovulation point, 10.12 vs 15.55; number of transferable embryos, 8.82 vs 13.15) at the 0.05 level. Both average ovulation point and number of transferable embryos were also significantly different between April -June and October- December ( average ovulation point, 9.05 vs 15.55; number of transferable embryos, 7.05 vs 13.15) at the 0.05 level. Super-ovulation effect was not significantly different between the two treatment frequencies. [ Conduslonl The FSH dosages and seasons have significant impact on super-ovu- lation, but repeat super-ovulation does not have the same impact. 展开更多
关键词 Super-ovulation GOAT follicle-stimulating hormone SEASONS
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