To explore the effects of freeze‒thaw cycles on the mechanical properties and crack evolution of fissured sandstone,biaxial compression experiments were carried out on sandstone subjected to freeze‒thaw cycles to char...To explore the effects of freeze‒thaw cycles on the mechanical properties and crack evolution of fissured sandstone,biaxial compression experiments were carried out on sandstone subjected to freeze‒thaw cycles to characterize the changes in the physical and mechanical properties of fissured sandstone caused by freeze‒thaw cycles.The crack evolution and crack change process on the surface of the fissured sandstone were recorded and analysed in detail via digital image technology(DIC).Numerical simulation was used to reveal the expansion process and damage mode of fine-scale cracks under the action of freeze‒thaw cycles,and the simulation results were compared and analysed with the experimental data to verify the reliability of the numerical model.The results show that the mass loss,porosity,peak stress and elastic modulus all increase with increasing number of freeze‒thaw cycles.With an increase in the number of freeze‒thaw cycles,a substantial change in displacement occurs around the prefabricated cracks,and a stress concentration appears at the crack tip.As new cracks continue to sprout at the tips of the prefabricated cracks until the microcracks gradually penetrate into the main cracks,the displacement cloud becomes obviously discontinuous,and the contours of the displacement field in the crack fracture damage area simply intersect with the prefabricated cracks to form an obvious fracture.The damage patterns of the fractured sandstone after freeze‒thaw cycles clearly differ,forming a symmetrical"L"-shaped damage pattern at zero freeze‒thaw cycles,a symmetrical"V"-shaped damage pattern at 10 freeze‒thaw cycles,and a"V"-shaped damage pattern at 20 freeze‒thaw cycles.After 20 freeze‒thaw cycles,a"V"-shaped destruction pattern and"L"-shaped destruction pattern are formed;after 30 freeze‒thaw cycles,an"N"-shaped destruction pattern is formed.This shows that the failure mode of fractured sandstone gradually becomes more complicated with an increasing number of freeze‒thaw cycles.The effects of freeze‒thaw cycles on the direction and rate of crack propagation are revealed through a temperature‒load coupled model,which provides an important reference for an in-depth understanding of the freeze‒thaw failure mechanisms of fractured rock masses.展开更多
The reasonable quantification of the concrete freezing environment on the Qinghai–Tibet Plateau(QTP) is the primary issue in frost resistant concrete design, which is one of the challenges that the QTP engineering ma...The reasonable quantification of the concrete freezing environment on the Qinghai–Tibet Plateau(QTP) is the primary issue in frost resistant concrete design, which is one of the challenges that the QTP engineering managers should take into account. In this paper, we propose a more realistic method to calculate the number of concrete freeze–thaw cycles(NFTCs) on the QTP. The calculated results show that the NFTCs increase as the altitude of the meteorological station increases with the average NFTCs being 208.7. Four machine learning methods, i.e., the random forest(RF) model, generalized boosting method(GBM), generalized linear model(GLM), and generalized additive model(GAM), are used to fit the NFTCs. The root mean square error(RMSE) values of the RF, GBM, GLM, and GAM are 32.3, 4.3, 247.9, and 161.3, respectively. The R^(2) values of the RF, GBM, GLM, and GAM are 0.93, 0.99, 0.48, and 0.66, respectively. The GBM method performs the best compared to the other three methods, which was shown by the results of RMSE and R^(2) values. The quantitative results from the GBM method indicate that the lowest, medium, and highest NFTC values are distributed in the northern, central, and southern parts of the QTP, respectively. The annual NFTCs in the QTP region are mainly concentrated at 160 and above, and the average NFTCs is 200 across the QTP. Our results can provide scientific guidance and a theoretical basis for the freezing resistance design of concrete in various projects on the QTP.展开更多
Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a m...Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a multifunctional F–T cycle system was developed to imitate the groundwater recharge in the subgrade during the freezing process and a large number of dynamic triaxial experiments were conducted after the F–T cycles. Some significant factors including the F–T cycle number, compaction degree, confining pressure, cyclic deviator stress, loading frequency, and water content were investigated for the resilient modulus of soils. The experimental results indicated that the dynamic resilient modulus of the subgrade was negatively correlated with the cyclic deviator stress, F–T cycle number, and initial water content, whereas the degree of compaction, confining pressure, and loading frequency could enhance the resilient modulus. Furthermore, a modified model considering the F–T cycle number and stress state was established to predict the dynamic resilient modulus. The calculated results of this modified model were very close to the experimental results. Consequently, calculation of the resilient modulus for F–T cycles considering the dynamic load was appropriate. This study provides reference for research focusing on F–T cycles with groundwater supply and the dynamic resilient moduli of subgrade soils in seasonally frozen areas.展开更多
Based on salt-frost heave tests of sulfate saline soil under repeated freeze−thaw cycles,this paper discusses the mechanism of the salt-frost heave under long-term freeze−thaw cycles.The results show that the salt-fro...Based on salt-frost heave tests of sulfate saline soil under repeated freeze−thaw cycles,this paper discusses the mechanism of the salt-frost heave under long-term freeze−thaw cycles.The results show that the salt-frost heave can be restricted considerably by loads,and there is a critical load for the salt-frost heave cumulative effect.Under this load,peak values of salt-frost heave approach a constant,and the residual values become 0.There is no longer structure heave or cumulative effect of saline soil exposed to freeze−thaw cycles under the critical load.Taking cumulative effect into account in calculations of salt-frost heave,a salt-frost heave model under freeze−thaw cycles is developed.展开更多
In the Loess Plateau in Northern China,repeated freeze–thaw(FT)cycles deteriorate the strength and structure of loess as a foundation soil,resulting in the instability or failure of supporting structure.Lignosulfonat...In the Loess Plateau in Northern China,repeated freeze–thaw(FT)cycles deteriorate the strength and structure of loess as a foundation soil,resulting in the instability or failure of supporting structure.Lignosulfonate is an eco–material,utilized as an effective and nontraditional stabilizer to improve the engineering properties of metastable soils.A series of laboratory tests,including unconfined compression tests,cyclic loading–unloading tests and scanning electron microscopy,on calcium lignosulfonate(CL)-and sodium lignosulfonate(SL)-stabilized loess were performed to investigate the stabilization effect,deterioration mechanisms of the FT cycles,and the resistance to FT cycles.Two traditional stabilizers,quicklime(QL)and sodium silicate(SS),were selected,and the engineering properties of QL-and SS-stabilized loess were compared with those of CL-and SLstabilized loess.The results showed that the strength values of CL-and SL-stabilized loess specimens decreased by 34.2%and 50%respectively,after 20 FT cycles,whereas those of the traditionally SS-and QL-stabilized specimens decreased by 85.3%and 82.87%,respectively.The elastic moduli of SL-and QL-stabilized loess specimens decreased by 22.1%and 92.0%,respectively.The mean energy dissipations of nontraditionally treated specimens also decreased significantly less than those of traditionally treated specimens.Overall,the results showed CL and SL had better stabilization effects on engineering properties of loess than QL and SS,and their stabilized loess specimens exhibited stronger resistance to FT cycles.The study findings demonstrated the significant potential of lignosulfonate for extensive application in cold loess areas.展开更多
The research of the failure criterion and one-dimensional stress-strain relationship of deteriorated concrete were carried out. Based on the damage mechanics theory, the dsmage which reflects the alternation of intern...The research of the failure criterion and one-dimensional stress-strain relationship of deteriorated concrete were carried out. Based on the damage mechanics theory, the dsmage which reflects the alternation of internal state of material were introduced into the formula presented by Desayi and Krishman and the weighted twin-shear strength theory. As a nondestructive examination method in common use, the ultrasonic technique was adopted in the study, and the ultrasonic velocity was used to establish the damage variable. After that, the failure criterion and one-dimensional stress-strain relationship for deteriorated concrete were obtained. Eventually, tests were carried out to study the evolution laws on the damage. The results show that the more freezing and thawing cycles are, the more apparently the failure surface shrinks. Meanwhile, the comparison between theoretical data and experimental data verifies tile rationality of tile damage-based one-dimensional stress-strain relationship proposed.展开更多
Background:Freeze–thaw events are common in boreal and temperate forest ecosystems and are increasingly infuenced by climate warming.Soil microorganisms play an important role in maintaining ecosystem stability,but t...Background:Freeze–thaw events are common in boreal and temperate forest ecosystems and are increasingly infuenced by climate warming.Soil microorganisms play an important role in maintaining ecosystem stability,but their responses to freeze–thaw cycles(FTCs)are poorly understood.We conducted a feld freeze–thaw experiment in a natural Korean pine and broadleaf mixed forest in the Changbai Mountain Nature Reserve,China,to determine the dynamic responses of soil microbial communities to FTCs.Results:Bacteria were more sensitive than fungi to FTCs.Fungal biomass,diversity and community composition were not signifcantly afected by freeze–thaw regardless of the stage.Moderate initial freeze–thaw resulted in increased bacterial biomass,diversity,and copiotrophic taxa abundance.Subsequent FTCs reduced the bacterial biomass and diversity.Compared with the initial FTC,subsequent FTCs exerted an opposite efect on the direction of change in the composition and function of the bacterial community.Soil water content,dissolved organic carbon,ammonium nitrogen,and total dissolved phosphorus were important factors determining bacterial community diversity and composition during FTCs.Moreover,the functional potentials of the microbial community involved in C and N cycling were also afected by FTCs.Conclusions:Diferent stages of FTCs have diferent ecological efects on the soil environment and microbial activities.Soil FTCs changed the soil nutrients and water availability and then mainly infuenced bacterial community composition,diversity,and functional potentials,which may disturb C and N states in this temperate forest soil.This study also improves our understanding of microbial communities regulating their ecological functions in response to climate change.展开更多
This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strengt...This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strength properties of coarse-grained soil by means of frost heaving tests and static triaxial tests, and the results are as follows: (1) the freezing temperature of coarse-grained soil decreased gradually and then leveled off with incremental increases in the percent content of fines; (2) the fines content proved to be an important factor influencing the frost heave susceptibility and strength properties of coarse-grained soil. With incremental increases in the percent content of fines, the frost heave ratio increased gradually and the cohesion function of fines effectively enhanced the shear strength of coarse-grained soil before freeze-thaw, but the frost susceptibility of fines weakened the shear strength of coarse-grained soil after freeze-thaw; (3) with increasing numbers of freeze-thaw cycles, the shear strength of coarse-grained soil decreased and then stabilized after the ninth freeze-thaw cycle, and therefore the mechanical indexes of the ninth freeze-thaw cycle are recommended for the engi- neering design values; and (4) considering frost susceptibility and strength properties as a whole, the optimal fines content of 5% is recommended for railway sub,fade coarse-~rained soil fillings in frozen re^ions.展开更多
Aims Alpine forest gaps can control understory ecosystem processes by manipulating hydrothermal dynamics.Here,we aimed to test the role of alpine forest gap disturbance on total phenol loss(TPL)from the decomposing li...Aims Alpine forest gaps can control understory ecosystem processes by manipulating hydrothermal dynamics.Here,we aimed to test the role of alpine forest gap disturbance on total phenol loss(TPL)from the decomposing litter of two typical shrub species(willow,Salix paraplesia Schneid.,and bamboo,Fargesia nitida(Mitford)Keng f.).Methods We conducted a field litterbag experiment within a representative fir(Abies faxoniana Rehd.)forest based on‘gap openness treatments’(plot positions in the gap included the gap center south,gap center north,canopy edge,expanded edge and closed canopy).The TPL rate and litter surface microbial abundance(fungi and bacteria)of the two shrub species were measured during the following periods over 2 years:snow formation(SF),snow cover(SC),snow melting(ST),the early growing season(EG)and the late growing season(LG).Important Findings At the end of the study,we found that snow cover depth,freeze–thaw cycle frequency and the fungal copies g−1 to bacterial copies g−1 ratio had significant effects on litter TPL.The abundances of fungi and bacteria decreased from the gap center to the closed canopy during the SF,SC,ST and LG periods and showed the opposite trend during the EG periods.The rate of TPL among plot positions closely followed the same trend as microbial abundance during the first year of incubation.In addition,both species had higher rates of TPL in the gap center than at other positions during the first winter,first year and entire 2-year period.These findings suggest that alpine forest gap formation accelerates litter TPL,although litter TPL exhibits dual responses to gap disturbance during specific critical periods.In conclusion,reduced snow cover depth and duration during winter warming under projected climate change scenarios or as gaps vanish may slow litter TPL in alpine biomes.展开更多
Functional gradient materials provided us a new concept for artificial articular cartilage design with gradient component and gradient structure where one side of the material is high free water content thereby provid...Functional gradient materials provided us a new concept for artificial articular cartilage design with gradient component and gradient structure where one side of the material is high free water content thereby providing excellent lubrication function and the opposite side of the material is high hydroxyapatite content, thereby improving the bioactivity of the material and stimulating cell growth. The goal of the present study was to develop a multi-layered gradient HA/PVA gel biocomposites through layer-by-layer casting method combing with freeze/thaw cycle technology. The various influence factors on the compressive strength and modulus of the multi-layered gradient biocomposites were investigated. The results showed that the compressive mechanical characteristics of the biocomposites were similar to that of natural articular cartilage. Both the compressive strength and modulus of the multi-layered gradient HA/PVA gel biocomposites increased exponentially with the rise of compressive strain ratio. Both the compressive strength and average compressive modulus of the biocomposites improved with the rise of freeze/thaw cycle times and total concentration of HA particles in the biocomposites, but they showed decreasing tendency with the rise of HA concentration difference between adjacent layers.展开更多
The aim of the research was to evaluate, at site scale, the influence of freezing and freeze/thaw cycles on the survival of faecal coliforms and faecal enterococci in soil, in a climate change perspective. Before the ...The aim of the research was to evaluate, at site scale, the influence of freezing and freeze/thaw cycles on the survival of faecal coliforms and faecal enterococci in soil, in a climate change perspective. Before the winter period and during grazing, viable cells of faecal coliforms and faecal enterococci were detected only in the first 10 cm below ground, while,after the winter period and before the new seasonal grazing, a lower number of viable cells of both faecal indicators was detected only in some of the investigated soil profiles, and within the first 5 cm. Taking into consideration the results of specific investigations, we hypothesise that the non-uniform spatial distribution of grass roots within the studied soil can play an important role in influencing this phenomenon, while several abiotic factors do not play any significant role. Taking into account the local trend in the increase of air temperature, a different distribution of microbial pollution over time is expected in spring waters, in future climate scenarios. The progressive increase in air temperature will cause a progressive decrease in freeze/thaw cycles at higher altitudes, minimising cold shocks on microbial cells, and causing spring water pollution also during winter.展开更多
基金supported by the National Natural Science Foundation of China(Project No.52074123).
文摘To explore the effects of freeze‒thaw cycles on the mechanical properties and crack evolution of fissured sandstone,biaxial compression experiments were carried out on sandstone subjected to freeze‒thaw cycles to characterize the changes in the physical and mechanical properties of fissured sandstone caused by freeze‒thaw cycles.The crack evolution and crack change process on the surface of the fissured sandstone were recorded and analysed in detail via digital image technology(DIC).Numerical simulation was used to reveal the expansion process and damage mode of fine-scale cracks under the action of freeze‒thaw cycles,and the simulation results were compared and analysed with the experimental data to verify the reliability of the numerical model.The results show that the mass loss,porosity,peak stress and elastic modulus all increase with increasing number of freeze‒thaw cycles.With an increase in the number of freeze‒thaw cycles,a substantial change in displacement occurs around the prefabricated cracks,and a stress concentration appears at the crack tip.As new cracks continue to sprout at the tips of the prefabricated cracks until the microcracks gradually penetrate into the main cracks,the displacement cloud becomes obviously discontinuous,and the contours of the displacement field in the crack fracture damage area simply intersect with the prefabricated cracks to form an obvious fracture.The damage patterns of the fractured sandstone after freeze‒thaw cycles clearly differ,forming a symmetrical"L"-shaped damage pattern at zero freeze‒thaw cycles,a symmetrical"V"-shaped damage pattern at 10 freeze‒thaw cycles,and a"V"-shaped damage pattern at 20 freeze‒thaw cycles.After 20 freeze‒thaw cycles,a"V"-shaped destruction pattern and"L"-shaped destruction pattern are formed;after 30 freeze‒thaw cycles,an"N"-shaped destruction pattern is formed.This shows that the failure mode of fractured sandstone gradually becomes more complicated with an increasing number of freeze‒thaw cycles.The effects of freeze‒thaw cycles on the direction and rate of crack propagation are revealed through a temperature‒load coupled model,which provides an important reference for an in-depth understanding of the freeze‒thaw failure mechanisms of fractured rock masses.
基金supported by Shandong Provincial Natural Science Foundation (grant number: ZR2023MD036)Key Research and Development Project in Shandong Province (grant number: 2019GGX101064)project for excellent youth foundation of the innovation teacher team, Shandong (grant number: 2022KJ310)。
文摘The reasonable quantification of the concrete freezing environment on the Qinghai–Tibet Plateau(QTP) is the primary issue in frost resistant concrete design, which is one of the challenges that the QTP engineering managers should take into account. In this paper, we propose a more realistic method to calculate the number of concrete freeze–thaw cycles(NFTCs) on the QTP. The calculated results show that the NFTCs increase as the altitude of the meteorological station increases with the average NFTCs being 208.7. Four machine learning methods, i.e., the random forest(RF) model, generalized boosting method(GBM), generalized linear model(GLM), and generalized additive model(GAM), are used to fit the NFTCs. The root mean square error(RMSE) values of the RF, GBM, GLM, and GAM are 32.3, 4.3, 247.9, and 161.3, respectively. The R^(2) values of the RF, GBM, GLM, and GAM are 0.93, 0.99, 0.48, and 0.66, respectively. The GBM method performs the best compared to the other three methods, which was shown by the results of RMSE and R^(2) values. The quantitative results from the GBM method indicate that the lowest, medium, and highest NFTC values are distributed in the northern, central, and southern parts of the QTP, respectively. The annual NFTCs in the QTP region are mainly concentrated at 160 and above, and the average NFTCs is 200 across the QTP. Our results can provide scientific guidance and a theoretical basis for the freezing resistance design of concrete in various projects on the QTP.
基金Projects(41672312, 41972294) supported by the National Natural Science Foundation of ChinaProject(2017CFA056) supported by the Outstanding Youth Foundation of Hubei Province, ChinaProject(KFJ170104) supported by the Changsha University of Science & Technology via Open Fund of National Engineering Laboratory of Highway Maintenance Technology, China。
文摘Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a multifunctional F–T cycle system was developed to imitate the groundwater recharge in the subgrade during the freezing process and a large number of dynamic triaxial experiments were conducted after the F–T cycles. Some significant factors including the F–T cycle number, compaction degree, confining pressure, cyclic deviator stress, loading frequency, and water content were investigated for the resilient modulus of soils. The experimental results indicated that the dynamic resilient modulus of the subgrade was negatively correlated with the cyclic deviator stress, F–T cycle number, and initial water content, whereas the degree of compaction, confining pressure, and loading frequency could enhance the resilient modulus. Furthermore, a modified model considering the F–T cycle number and stress state was established to predict the dynamic resilient modulus. The calculated results of this modified model were very close to the experimental results. Consequently, calculation of the resilient modulus for F–T cycles considering the dynamic load was appropriate. This study provides reference for research focusing on F–T cycles with groundwater supply and the dynamic resilient moduli of subgrade soils in seasonally frozen areas.
基金the General project of Chongqing Science and Technology Commission Scientific(Grant No.cstc2019jcyj-msxmX0813)Youth Program of Scientific and Technological Innovation Fund of Gansu Academy of Sciences(Grant No.2019QN-04)and is a part of the Scientific Project of Yangtze Normal University(Grant No.2017 XJQN16).
文摘Based on salt-frost heave tests of sulfate saline soil under repeated freeze−thaw cycles,this paper discusses the mechanism of the salt-frost heave under long-term freeze−thaw cycles.The results show that the salt-frost heave can be restricted considerably by loads,and there is a critical load for the salt-frost heave cumulative effect.Under this load,peak values of salt-frost heave approach a constant,and the residual values become 0.There is no longer structure heave or cumulative effect of saline soil exposed to freeze−thaw cycles under the critical load.Taking cumulative effect into account in calculations of salt-frost heave,a salt-frost heave model under freeze−thaw cycles is developed.
基金funded by the China’s Second Tibetan Plateau Scientific Expedition and Research(2019QZKK0905)the National Natural Science Foundation of China(Nos.41672310 and U1703244)+1 种基金the Science and Technology Major Project of Gansu Province(143GKDA007)Research Project of the State Key Laboratory of Frozen Soils Engineering(SKLFSE-ZY-20)。
文摘In the Loess Plateau in Northern China,repeated freeze–thaw(FT)cycles deteriorate the strength and structure of loess as a foundation soil,resulting in the instability or failure of supporting structure.Lignosulfonate is an eco–material,utilized as an effective and nontraditional stabilizer to improve the engineering properties of metastable soils.A series of laboratory tests,including unconfined compression tests,cyclic loading–unloading tests and scanning electron microscopy,on calcium lignosulfonate(CL)-and sodium lignosulfonate(SL)-stabilized loess were performed to investigate the stabilization effect,deterioration mechanisms of the FT cycles,and the resistance to FT cycles.Two traditional stabilizers,quicklime(QL)and sodium silicate(SS),were selected,and the engineering properties of QL-and SS-stabilized loess were compared with those of CL-and SLstabilized loess.The results showed that the strength values of CL-and SL-stabilized loess specimens decreased by 34.2%and 50%respectively,after 20 FT cycles,whereas those of the traditionally SS-and QL-stabilized specimens decreased by 85.3%and 82.87%,respectively.The elastic moduli of SL-and QL-stabilized loess specimens decreased by 22.1%and 92.0%,respectively.The mean energy dissipations of nontraditionally treated specimens also decreased significantly less than those of traditionally treated specimens.Overall,the results showed CL and SL had better stabilization effects on engineering properties of loess than QL and SS,and their stabilized loess specimens exhibited stronger resistance to FT cycles.The study findings demonstrated the significant potential of lignosulfonate for extensive application in cold loess areas.
文摘The research of the failure criterion and one-dimensional stress-strain relationship of deteriorated concrete were carried out. Based on the damage mechanics theory, the dsmage which reflects the alternation of internal state of material were introduced into the formula presented by Desayi and Krishman and the weighted twin-shear strength theory. As a nondestructive examination method in common use, the ultrasonic technique was adopted in the study, and the ultrasonic velocity was used to establish the damage variable. After that, the failure criterion and one-dimensional stress-strain relationship for deteriorated concrete were obtained. Eventually, tests were carried out to study the evolution laws on the damage. The results show that the more freezing and thawing cycles are, the more apparently the failure surface shrinks. Meanwhile, the comparison between theoretical data and experimental data verifies tile rationality of tile damage-based one-dimensional stress-strain relationship proposed.
基金The National Natural Science Foundation of China(31770531,32001174)the Key Research Program of Frontier Sciences,CAS(QYZDB-SSW-DQC006)+1 种基金the Key Laboratory of Geographical Processes and Ecological Security of Changbai Mountains,Ministry of Education(GPES201902)the Youth Innovation Promotion Association CAS to Chao Wang(2018231).
文摘Background:Freeze–thaw events are common in boreal and temperate forest ecosystems and are increasingly infuenced by climate warming.Soil microorganisms play an important role in maintaining ecosystem stability,but their responses to freeze–thaw cycles(FTCs)are poorly understood.We conducted a feld freeze–thaw experiment in a natural Korean pine and broadleaf mixed forest in the Changbai Mountain Nature Reserve,China,to determine the dynamic responses of soil microbial communities to FTCs.Results:Bacteria were more sensitive than fungi to FTCs.Fungal biomass,diversity and community composition were not signifcantly afected by freeze–thaw regardless of the stage.Moderate initial freeze–thaw resulted in increased bacterial biomass,diversity,and copiotrophic taxa abundance.Subsequent FTCs reduced the bacterial biomass and diversity.Compared with the initial FTC,subsequent FTCs exerted an opposite efect on the direction of change in the composition and function of the bacterial community.Soil water content,dissolved organic carbon,ammonium nitrogen,and total dissolved phosphorus were important factors determining bacterial community diversity and composition during FTCs.Moreover,the functional potentials of the microbial community involved in C and N cycling were also afected by FTCs.Conclusions:Diferent stages of FTCs have diferent ecological efects on the soil environment and microbial activities.Soil FTCs changed the soil nutrients and water availability and then mainly infuenced bacterial community composition,diversity,and functional potentials,which may disturb C and N states in this temperate forest soil.This study also improves our understanding of microbial communities regulating their ecological functions in response to climate change.
基金supported by the National Key Technology Support Program of China (No.2012BAG05B00)the National Natural Science Foundation of China (Nos. 51208320 and 51178281)the Key Subject of China Railway Corporation (Nos. 2014G003-F and 2014G003-A)
文摘This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strength properties of coarse-grained soil by means of frost heaving tests and static triaxial tests, and the results are as follows: (1) the freezing temperature of coarse-grained soil decreased gradually and then leveled off with incremental increases in the percent content of fines; (2) the fines content proved to be an important factor influencing the frost heave susceptibility and strength properties of coarse-grained soil. With incremental increases in the percent content of fines, the frost heave ratio increased gradually and the cohesion function of fines effectively enhanced the shear strength of coarse-grained soil before freeze-thaw, but the frost susceptibility of fines weakened the shear strength of coarse-grained soil after freeze-thaw; (3) with increasing numbers of freeze-thaw cycles, the shear strength of coarse-grained soil decreased and then stabilized after the ninth freeze-thaw cycle, and therefore the mechanical indexes of the ninth freeze-thaw cycle are recommended for the engi- neering design values; and (4) considering frost susceptibility and strength properties as a whole, the optimal fines content of 5% is recommended for railway sub,fade coarse-~rained soil fillings in frozen re^ions.
基金supported by the National Natural Science Foundation of China(no.31570445 and 31800518).
文摘Aims Alpine forest gaps can control understory ecosystem processes by manipulating hydrothermal dynamics.Here,we aimed to test the role of alpine forest gap disturbance on total phenol loss(TPL)from the decomposing litter of two typical shrub species(willow,Salix paraplesia Schneid.,and bamboo,Fargesia nitida(Mitford)Keng f.).Methods We conducted a field litterbag experiment within a representative fir(Abies faxoniana Rehd.)forest based on‘gap openness treatments’(plot positions in the gap included the gap center south,gap center north,canopy edge,expanded edge and closed canopy).The TPL rate and litter surface microbial abundance(fungi and bacteria)of the two shrub species were measured during the following periods over 2 years:snow formation(SF),snow cover(SC),snow melting(ST),the early growing season(EG)and the late growing season(LG).Important Findings At the end of the study,we found that snow cover depth,freeze–thaw cycle frequency and the fungal copies g−1 to bacterial copies g−1 ratio had significant effects on litter TPL.The abundances of fungi and bacteria decreased from the gap center to the closed canopy during the SF,SC,ST and LG periods and showed the opposite trend during the EG periods.The rate of TPL among plot positions closely followed the same trend as microbial abundance during the first year of incubation.In addition,both species had higher rates of TPL in the gap center than at other positions during the first winter,first year and entire 2-year period.These findings suggest that alpine forest gap formation accelerates litter TPL,although litter TPL exhibits dual responses to gap disturbance during specific critical periods.In conclusion,reduced snow cover depth and duration during winter warming under projected climate change scenarios or as gaps vanish may slow litter TPL in alpine biomes.
基金the financial support from the National Natural Science Foundation of China (No.51175004)the Natural Science Research of Key Projects of Anhui Provincial Universities(No.KJ2010A099)
文摘Functional gradient materials provided us a new concept for artificial articular cartilage design with gradient component and gradient structure where one side of the material is high free water content thereby providing excellent lubrication function and the opposite side of the material is high hydroxyapatite content, thereby improving the bioactivity of the material and stimulating cell growth. The goal of the present study was to develop a multi-layered gradient HA/PVA gel biocomposites through layer-by-layer casting method combing with freeze/thaw cycle technology. The various influence factors on the compressive strength and modulus of the multi-layered gradient biocomposites were investigated. The results showed that the compressive mechanical characteristics of the biocomposites were similar to that of natural articular cartilage. Both the compressive strength and modulus of the multi-layered gradient HA/PVA gel biocomposites increased exponentially with the rise of compressive strain ratio. Both the compressive strength and average compressive modulus of the biocomposites improved with the rise of freeze/thaw cycle times and total concentration of HA particles in the biocomposites, but they showed decreasing tendency with the rise of HA concentration difference between adjacent layers.
基金supported by the European Union (CC-Water S project, SEE/A/022/2.1/X)by the Ministero dell'Istruzione, dell'Università e della Ricerca (PRIN2008TL25YL)
文摘The aim of the research was to evaluate, at site scale, the influence of freezing and freeze/thaw cycles on the survival of faecal coliforms and faecal enterococci in soil, in a climate change perspective. Before the winter period and during grazing, viable cells of faecal coliforms and faecal enterococci were detected only in the first 10 cm below ground, while,after the winter period and before the new seasonal grazing, a lower number of viable cells of both faecal indicators was detected only in some of the investigated soil profiles, and within the first 5 cm. Taking into consideration the results of specific investigations, we hypothesise that the non-uniform spatial distribution of grass roots within the studied soil can play an important role in influencing this phenomenon, while several abiotic factors do not play any significant role. Taking into account the local trend in the increase of air temperature, a different distribution of microbial pollution over time is expected in spring waters, in future climate scenarios. The progressive increase in air temperature will cause a progressive decrease in freeze/thaw cycles at higher altitudes, minimising cold shocks on microbial cells, and causing spring water pollution also during winter.