Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock ...Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins （MgsHSP22 and MgsHSP24.1） were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 ug/L Cd2＋ exposure. MgsHSP24.1 expression was also significantly inhibited after 50 ug/L Cd2＋ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2＋ stress （5 and 50 ug/L）, while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.展开更多
In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, M ytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thaw...In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, M ytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thawing temperature, sugar addition and sperm to oocyte ratio. The sperm quality was assessed by the fertilization rate or the integrity of sperm component and organelle. The highest post-thaw sperm fertilization rate was 91%, which was produced with sperm cryopreserved in 8% dimethyl sulfoxide at the cooling rate of-4°C/min from 2°C to-30°C before being plunged into liquid nitrogen for at least 12 h, thawed in a 20°C seawater bath and fertilized at sperm to egg ratio of 50 000:1. The addition of glucose, sucrose or trehalose to 8% dimethyl sulfoxide could not further improve fertilization rates. The fluorescent assessments showed that the post-thaw sperm plasma membrane integrity and acrosome integrity were significantly damaged in comparison with fresh sperm.展开更多
Mussel samples from the Dakar coast (Senegal) were analysed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Microwave acid digestion was also employed for metals (Mn, Pb, Cd, As) determination. Dakar coas...Mussel samples from the Dakar coast (Senegal) were analysed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Microwave acid digestion was also employed for metals (Mn, Pb, Cd, As) determination. Dakar coast usually receives numerous domestic and industrial discharges without prior treatment. The contents of Arsenic were, in all cases, higher than other metals. However, the bivalve molluscs present themselves as effective bio-monitors when assessing marine aquatic pollution by contaminants in the Dakar coast. The ANOVA analysis allows concluding that significant differences were found between mussels from different sampling points. In all cases, the February samples have in all cases a higher content than those collected in October.展开更多
Butyltin compounds (BTs) including tributyltin (TBT) and its degradation product (DBT) and concentrations of heavy metals (Sn, Cu and Zn) were determined in mussels (Mytilus galloprovincialis) collected from some West...Butyltin compounds (BTs) including tributyltin (TBT) and its degradation product (DBT) and concentrations of heavy metals (Sn, Cu and Zn) were determined in mussels (Mytilus galloprovincialis) collected from some West Algerian harbours. BTs were detected in all the mussels. Quanti?cation of BTs and heavy metals was carried respectively by Gas Chromatography coupled with Mass Spectrometer (GC-MS) and Atomic Absorption Spectrometry, flame AAS. Concentrations of total butyltin (BTs: DBT + TBT) in mussels ranged from 0.49 to 2438 ng/g wet wt. The concentrations (μg/g wet wt) of heavy metals ranged from 0.40 to 3.55 for Sn, 9.62 to 67.03 for Cu, and 87.13 to 731.51 for Zn. Total of tin in mussels ranged from 0.2 to 1054.78 ng/g. Higher concentrations of butyltin compounds were found in mussels collected from Mers El Kebir shipyard, Arzew, a petroleum harbour (industrial), and Beni Saf fishing harbour. This suggested that maritime activities nearby the harbours play a major role as the source of BTs. Indeed, harbour of Arzew is frequented by voluminous tanker. TBT was the predominant compound in mussels collected from almost all the sampling locations;indicate the fresh input of TBT in harbours.展开更多
为探究海州湾不同养殖区紫贻贝(Mytilusgalloprovincialis)收获期营养成分变化及其影响因素,于2021年1—3月测定了海州湾不同养殖区(H1:离岸1 n mile,H2:离岸3 n mile,H3:离岸7 n mile、H4:离岸13 n mile,H5:离岸21 n mile)水环境理化...为探究海州湾不同养殖区紫贻贝(Mytilusgalloprovincialis)收获期营养成分变化及其影响因素,于2021年1—3月测定了海州湾不同养殖区(H1:离岸1 n mile,H2:离岸3 n mile,H3:离岸7 n mile、H4:离岸13 n mile,H5:离岸21 n mile)水环境理化因子、紫贻贝的条件指数及蛋白质、脂肪、糖原、氨基酸等营养成分,分析了水体理化因子与条件指数和营养成分的关系。结果显示,随着离岸距离的增加,水温和叶绿素a(Chl-a)浓度等关键理化因子均有升高趋势,1—3月H1站位紫贻贝的条件指数逐渐增加,H2~H5站位的条件指数均在2月达到峰值;各站位的紫贻贝总糖含量呈正态分布,于2月达到峰值,其中,H4和H5站位总糖含量在3月显著下降(P<0.05),比2月分别降低了62.7%和61.6%;H5站位紫贻贝的氨基酸含量在1月显著高于其他站位(P<0.05),而后,在3月显著降为各站位中最低(P<0.05);1—3月各站位紫贻贝外套膜的糖原含量呈先上升后下降的趋势,而3月H5站位紫贻贝糖原含量显著高于其他站位。本研究表明,紫贻贝营养物质储备的差异主要与水温和饵料密度有关,离岸养殖更有助于紫贻贝积累营养物质及生长,离岸养殖区紫贻贝性腺成熟早,营养物质积累快,H5站位紫贻贝比近岸站位可提早1个月上市。本研究将为拓展紫贻贝养殖空间、优化养殖布局提供数据参考。展开更多
基金Supported by the 100 Talents Program of the Chinese Academy of Sciencesthe National Natural Science Foundation of China(No.41206105)the Key Deployment Program of Chinese Academy of Sciences(No.KZZD-EW-14-03)
文摘Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins （MgsHSP22 and MgsHSP24.1） were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 ug/L Cd2＋ exposure. MgsHSP24.1 expression was also significantly inhibited after 50 ug/L Cd2＋ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2＋ stress （5 and 50 ug/L）, while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.
基金Supported by the China Scholarship Council and South Australian Research and Development Institute(SARDI)
文摘In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, M ytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thawing temperature, sugar addition and sperm to oocyte ratio. The sperm quality was assessed by the fertilization rate or the integrity of sperm component and organelle. The highest post-thaw sperm fertilization rate was 91%, which was produced with sperm cryopreserved in 8% dimethyl sulfoxide at the cooling rate of-4°C/min from 2°C to-30°C before being plunged into liquid nitrogen for at least 12 h, thawed in a 20°C seawater bath and fertilized at sperm to egg ratio of 50 000:1. The addition of glucose, sucrose or trehalose to 8% dimethyl sulfoxide could not further improve fertilization rates. The fluorescent assessments showed that the post-thaw sperm plasma membrane integrity and acrosome integrity were significantly damaged in comparison with fresh sperm.
文摘Mussel samples from the Dakar coast (Senegal) were analysed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Microwave acid digestion was also employed for metals (Mn, Pb, Cd, As) determination. Dakar coast usually receives numerous domestic and industrial discharges without prior treatment. The contents of Arsenic were, in all cases, higher than other metals. However, the bivalve molluscs present themselves as effective bio-monitors when assessing marine aquatic pollution by contaminants in the Dakar coast. The ANOVA analysis allows concluding that significant differences were found between mussels from different sampling points. In all cases, the February samples have in all cases a higher content than those collected in October.
文摘Butyltin compounds (BTs) including tributyltin (TBT) and its degradation product (DBT) and concentrations of heavy metals (Sn, Cu and Zn) were determined in mussels (Mytilus galloprovincialis) collected from some West Algerian harbours. BTs were detected in all the mussels. Quanti?cation of BTs and heavy metals was carried respectively by Gas Chromatography coupled with Mass Spectrometer (GC-MS) and Atomic Absorption Spectrometry, flame AAS. Concentrations of total butyltin (BTs: DBT + TBT) in mussels ranged from 0.49 to 2438 ng/g wet wt. The concentrations (μg/g wet wt) of heavy metals ranged from 0.40 to 3.55 for Sn, 9.62 to 67.03 for Cu, and 87.13 to 731.51 for Zn. Total of tin in mussels ranged from 0.2 to 1054.78 ng/g. Higher concentrations of butyltin compounds were found in mussels collected from Mers El Kebir shipyard, Arzew, a petroleum harbour (industrial), and Beni Saf fishing harbour. This suggested that maritime activities nearby the harbours play a major role as the source of BTs. Indeed, harbour of Arzew is frequented by voluminous tanker. TBT was the predominant compound in mussels collected from almost all the sampling locations;indicate the fresh input of TBT in harbours.
文摘为探究海州湾不同养殖区紫贻贝(Mytilusgalloprovincialis)收获期营养成分变化及其影响因素,于2021年1—3月测定了海州湾不同养殖区(H1:离岸1 n mile,H2:离岸3 n mile,H3:离岸7 n mile、H4:离岸13 n mile,H5:离岸21 n mile)水环境理化因子、紫贻贝的条件指数及蛋白质、脂肪、糖原、氨基酸等营养成分,分析了水体理化因子与条件指数和营养成分的关系。结果显示,随着离岸距离的增加,水温和叶绿素a(Chl-a)浓度等关键理化因子均有升高趋势,1—3月H1站位紫贻贝的条件指数逐渐增加,H2~H5站位的条件指数均在2月达到峰值;各站位的紫贻贝总糖含量呈正态分布,于2月达到峰值,其中,H4和H5站位总糖含量在3月显著下降(P<0.05),比2月分别降低了62.7%和61.6%;H5站位紫贻贝的氨基酸含量在1月显著高于其他站位(P<0.05),而后,在3月显著降为各站位中最低(P<0.05);1—3月各站位紫贻贝外套膜的糖原含量呈先上升后下降的趋势,而3月H5站位紫贻贝糖原含量显著高于其他站位。本研究表明,紫贻贝营养物质储备的差异主要与水温和饵料密度有关,离岸养殖更有助于紫贻贝积累营养物质及生长,离岸养殖区紫贻贝性腺成熟早,营养物质积累快,H5站位紫贻贝比近岸站位可提早1个月上市。本研究将为拓展紫贻贝养殖空间、优化养殖布局提供数据参考。
