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Effects of Polysaccharides from Pulsatilla Decoction on the Microvascular Endothelial Glycocalyx 被引量:5
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作者 ZHANG Tao WU Shuang +2 位作者 SUN Xiong DUAN Hui-qin MU Xiang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第11期2558-2561,共4页
Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the role... Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction (PPD) on the microvascular endothelial glycocalyx (eGC). The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were ifxed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD signiifcantly increased its thickness from (21.85±1.87) to (28.71±3.61) nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by inlfuencing the eGC. 展开更多
关键词 Pulsatilla decoction herbal polysaccharides glycocalyx microvascular endothelial cells jejunal mucosa transmission electron microscope
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Organization and Ultra-Structural Components of Endothelial Surface Glycocalyx Revealed by Stochastic Optical Reconstruction Microscopy(STORM)
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作者 Jie Fan Yi Sun +2 位作者 Yifan Xia John M.Tarbell Bingmei M.Fua 《医用生物力学》 EI CAS CSCD 北大核心 2019年第A01期6-7,共2页
Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the hom... Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the homeostasis of the circulatory system.In addition to forming a transport barrier between the blood and vessel wall,vascular ECs play important roles in regulating circulation functions.Besides biochemical stimuli,blood flow induced(hemodynamic)mechanical stimuli,such as shear stress,pressure and circumferential stretch,modulate EC morphology and functions by activating mechanosensors,signaling pathways,and gene and protein expressions.The EC responses to the hemodynamic forces(mechano-sensing and transduction)are critical to maintaining normal vascular functions.Failure in the mechano-sensing and transduction leads to serious vascular diseases including hypertension,atherosclerosis,aneurysms and thrombosis,to name a few[1].On the luminal surface of our blood vessels,there is a thin layer called endothelial surface glycocalyx(ESG)which consists of proteoglycans,glycosaminoglycans(GAGs)and glycoproteins.The GAGs in the ESG are heparan sulfate(HS),hyaluronic acid(HA),chondroitin sulfate(CS),and sialic acid(SA)[2].In order to play important roles in vascular functions,such as being a mechanosensor and transducer for the endothelial cells(ECs)to sense the blood flow,a molecular sieve to maintain normal microvessel permeability and a barrier between the circulating cells and endothelial cells forming the vessel wall,the ESG should have an organized structure at the molecular level.Due to the limitations of optical and electron microscopy,the ultra-structure and organization of ESG has not been revealed until recent development of a super high resolution fluorescence optical microscope,STORM(Stochastic Optical Reconstruction Microscopy).The diffraction of a single fluorescence molecule can be described as the point spread function(PSF).When the light of wavelengthλexcites the fluorophore(emitter),the intensity profile of the spot is defined as the PSF with the width^0.6λ/NA,NA is the numerical aperture of the objective.The diffraction-limited image resolution,for a high numerical aperture objective lens,is^200 nm in the lateral direction and^500 nm in the axial direction,for a conventional fluorescence microscope.The key idea of the single-molecule localization microscopy is to light the molecule,in turn,to achieve the nanometer-level accuracy of their position and reconstruction into a super-resolution image,such as STORM.STORM employs photo-switching mechanisms to stochastically activate individual molecules(photo-switchable or photoactivatable fluorophores)within the diffraction-limited region at different times.Then images with sub-diffraction limit resolution are reconstructed from the measured positions of individual fluorophores[3].To trade the super spatial resolution(accuracy),STORM sacrifices its temporal resolution(efficiency)by switching the state and sequentially exciting the emitters at a high density.Rust et al[3]employed organic dyes and fluorescent proteins as photo-switchable emitters to trade temporal resolution for a super spatial resolution(~20 nm lateral and^50 nm axial at present,can go down to a couple of nanometers if using smaller peptides or antibody fragments instead of currently used whole anti-bodies),which is an order of magnitude higher than conventional confocal microscopy.In the current study,we employed STORM to reveal the major ultra-structural components of the ESG,HS and HA,and their organization at the surface of the cultured EC monolayer[4].Materials and methods We used newly acquired Nikon-STORM system to observe the ESG on in vitro EC(bEnd3,mouse brain microvascular endothelial cells)monolayers.After confluency,the bEnd3 cells were immunolabeled with anti-HS,fol-lowed by an ATT0488 conjugated goat anti-mouse IgG,and with biotinylated HA binding protein,followed by an AF647 conjugated anti-biotin.The ESG was then imaged by the STORM with a 100x/1.49 oil immersed lens.Multiple Reporters of ATT0488 and AF647 with alternating illumination were used to acquire the 3D images of HS and HA.The field of 256×256(40×40μm2)of HS and HA at the surface of ECs was obtained based on totally 40,000 of EM-CCD captured images for each reporter at a capturing speed of 19 ms/frame.Results HA is a long molecule weaving into a network which covers the endothelial luminal surface.The diameter of the HA segments is 185.3±44.7 nm,155.5±57.2 nm,and 156.9±56.1 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.In contrast,HS is a shorter molecule,perpendicular to the cell surface.HA and HS are partially overlapped with each other at the endothelial luminal surface.We quantified the length,diameter,orientation,and density of HS at the top,middle and bottom regions of the endothelial surface.The diameter of the observed HS is 191.0±46.0 nm,284.3±71.1 nm,and 184.2±59.6 nm,and the length of the HS is 621.0±75.7 nm,651.0±118.0 nm,and 575.2±105.6 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.For the HS orientation,its angle with the cell surface is 92.9±1.9,88.7±8.2,and 96.2±10.9 degree,respectively,at the top,middle and bottom regions.The angle of 90 degree is perfectly perpendicular to the cell surface.For the HS distribution,the average density is0.398 elements/μm2,0.345 elements/μm2 and 0.665 elements/μm2,respectively,and the distance between the adjacent HS is 1 694.4±628.1 nm,1 844.8±758.5 nm,and 1 221.9±450.7 nm,respectively,at the top,middle and bottom regions.Conclusions Our results suggest that HS plays a major role in mechanosensing and HA plays a major role in the molecular sieve,due to their organization,ultra-structure and distribution. 展开更多
关键词 ORGANIZATION Ultra-Structural Components ENDOTHELIAL Surface glycocalyx REVEALED Optical Reconstruction Microscopy STORM
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Early diabetic kidney disease:Focus on the glycocalyx
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作者 Hui Yu Yi-Yun Song Xian-Hua Li 《World Journal of Diabetes》 SCIE 2023年第5期460-480,共21页
The incidence of diabetic kidney disease(DKD)is sharply increasing worldwide.Microalbuminuria is the primary clinical marker used to identify DKD,and its initiating step in diabetes is glomerular endothelial cell dysf... The incidence of diabetic kidney disease(DKD)is sharply increasing worldwide.Microalbuminuria is the primary clinical marker used to identify DKD,and its initiating step in diabetes is glomerular endothelial cell dysfunction,particularly glycocalyx impairment.The glycocalyx found on the surface of glomerular endothelial cells,is a dynamic hydrated layer structure composed of proteoglycans,glycoproteins,and some adsorbed soluble components.It reinforces the negative charge barrier,transduces the shear stress,and mediates the interaction of blood corpuscles and podocytes with endothelial cells.In the highglucose environment of diabetes,excessive reactive oxygen species and proinflammatory cytokines can damage the endothelial glycocalyx(EG)both directly and indirectly,which induces the production of microalbuminuria.Further research is required to elucidate the role of the podocyte glycocalyx,which may,together with endothelial cells,form a line of defense against albumin filtration.Interestingly,recent research has confirmed that the negative charge barrier function of the glycocalyx found in the glomerular basement membrane and its repulsion effect on albumin is limited.Therefore,to improve the early diagnosis and treatment of DKD,the potential mechanisms of EG degradation must be analyzed and more responsive and controllable targets must be explored.The content of this review will provide insights for future research. 展开更多
关键词 glycocalyx Diabetic kidney disease Endothelial cells Reactive oxygen species MICROALBUMINURIA ENZYME
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Hyaluronidase Proof for Endothelial Glycocalyx as Partaker of Microcirculation Disturbances
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作者 Alexander Maksimenko Askar Turashev +2 位作者 Anatoly Rogoza Elena Tischenko Andrey Fedorovicht 《Journal of Life Sciences》 2013年第2期171-188,共18页
Covalent modification of bovine testicular hyaluronidase with chondroitin sulphate led to changes in the pattern of glycation of native and modified enzyme in its reaction with neutral saccharides and N-acetylhexosami... Covalent modification of bovine testicular hyaluronidase with chondroitin sulphate led to changes in the pattern of glycation of native and modified enzyme in its reaction with neutral saccharides and N-acetylhexosamines. Thus, mono- and di-saccharides inactivated the native hyaluronidase to a greater extent than the chondroitin sulfate-modified enzyme. N-acetylhexosamine, on the opposite, inactivated the modified hyaluronidase to a greater extent than the native one. These properties made it possible to use native and modified hyaluronidase as an informative research system for in vivo measurement of the predominant type of saccharide agents in the circulation. The proposed approach was experimentally substantiated by obtained results of the study on these interactions of hyaluronidase derivatives with hyaluronan fragments and their mixture. In a model of post-ischemic perfusion of the rat limb, the effect of hyaluronidase derivatives and their components on restoration of the microcirculation were tracked using laser Doppler flowmetry. Native hyaluronidase accelerated the restoration of initial level of microcirculation, but modified enzyme was markedly inhibited by glycocalyx degradation products. N-acetylhexosamine was positioned at the reducing terminal of these products as a natural label for these glycocalyx fragments. These and other data obtained under various experimental conditions supported the participation of endothelial glycocalyx in microcirculation disturbances. 展开更多
关键词 MICROCIRCULATION vascular biology endothelial glycocalyx chondroitin sulfate HYALURONIDASE laser Doppler flowmetry.
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Resuscitation fluids as drugs:targeting the endothelial glycocalyx 被引量:5
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作者 Guangjian Wang Hongmin Zhang +2 位作者 Dawei Liu Xiaoting Wang Chinese Critical Ultrasound Study Group 《Chinese Medical Journal》 SCIE CAS CSCD 2022年第2期137-144,共8页
Fluid resuscitation is an essential intervention in critically ill patients,and its ultimate goal is to restore tissue perfusion.Critical illnesses are often accompanied by glycocalyx degradation caused by inflammator... Fluid resuscitation is an essential intervention in critically ill patients,and its ultimate goal is to restore tissue perfusion.Critical illnesses are often accompanied by glycocalyx degradation caused by inflammatory reactions,hypoperfusion,shock,and so forth,leading to disturbed microcirculatory perfusion and organ dysfunction.Therefore,maintaining or even restoring the glycocalyx integrity may be of high priority in the therapeutic strategy.Like drugs,however,different resuscitation fluids may have beneficial or harmful effects on the integrity of the glycocalyx.The purpose of this article is to review the effects of different resuscitation fluids on the glycocalyx.Many animal studies have shown that normal saline might be associated with glycocalyx degradation,but clinical studies have not confirmed this finding.Hydroxyethyl starch(HES),rather than other synthetic colloids,may restore the glycocalyx.However,the use of HES also leads to serious adverse events such as acute kidney injury and bleeding tendencies.Some studies have suggested that albumin may restore the glycocalyx,whereas others have suggested that balanced crystalloids might aggravate glycocalyx degradation.Notably,most studies did not correct the effects of the infusion rate or fluid volume;therefore,the results of using balanced crystalloids remain unclear.Moreover,mainly animal studies have suggested that plasma may protect and restore glycocalyx integrity,and this still requires confirmation by high-quality clinical studies. 展开更多
关键词 Fluid resuscitation Resuscitation fluid Fluid therapy Endothelial glycocalyx glycocalyx
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Endothelial glycocalyx as a potential theriapeutic target in organ injuries 被引量:7
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作者 Rui-Na Cao Li Tang +1 位作者 Zhong-Yuan Xia Rui Xia 《Chinese Medical Journal》 SCIE CAS CSCD 2019年第8期963-975,共13页
Objective:The endothelial glycocalyx(eGC)is a dynamic and multicomponent layer of macromolecules found at the surface of vascular endothelium,which is largely underappreciated.It has recently been recognized that eGC ... Objective:The endothelial glycocalyx(eGC)is a dynamic and multicomponent layer of macromolecules found at the surface of vascular endothelium,which is largely underappreciated.It has recently been recognized that eGC is a major regulator of endothelial function and may have therapeutic value in organ injuries.This study aimed to explore the role of the eGC in various pathologic and physiologic conditions,by reviewing the basic research findings pertaining to the detection of the eGC and its clinical significance.We also explored different pharmacologic agents used to protect and rebuild the eGC.Data sources:An in-depth search was performed in the PubMed database,focusing on research published after 2003 with keywords including eGC,permeability,glycocalyx and injuries,and glycocalyx protection.Study selection:Several authoritative reviews and original studies were identified and reviewed to summarize the characteristics of the eGC under physiologic and pathologic conditions as well as the detection and protection of the eGC.Results:The eGC degradation is closely associated with pathophysiologic changes such as vascular permeability,edema formation,mechanotransduction,and clotting cascade,together with neutrophil and platelet adhesion in diverse injury and disease states including inflammation(sepsis and trauma),ischemia-reperfusion injury,shock,hypervolemia,hypertension,hyperglycemia,and high Na+as well as diabetes and atherosclerosis.Therapeutic strategies for protecting and rebuilding the eGC should be explored through experimental test and clinical verifications.Conclusions:Disturbance of the eGC usually occurs at early stages of various clinical pathophysiologies which can be partly prevented and reversed by protecting and restoring the eGC.The eGC seems to be a promising diagnostic biomarker and therapeutic target in clinical settings. 展开更多
关键词 ENDOTHELIAL glycocalyx PERMEABILITY INJURIES glycocalyx protection
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Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis 被引量:12
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作者 Wen-qiao YU Shao-yang ZHANG +6 位作者 Shui-qiao FU Qing-hui FU Wei-na LU Jian ZHANG Zhong-yan LIANG Yun ZHANG Ting-bo LIANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第4期355-362,共8页
Objective: This study demonstrated that dexamethasone(DEX) protects the endothelial glycocalyx from damage induced by the inflammatory stimulus tumor necrosis factor-α(TNF-α) during severe acute pancreatitis(SAP), a... Objective: This study demonstrated that dexamethasone(DEX) protects the endothelial glycocalyx from damage induced by the inflammatory stimulus tumor necrosis factor-α(TNF-α) during severe acute pancreatitis(SAP), and improves the renal microcirculation. Methods: Ninety mice were evenly divided into 3 groups(Sham, SAP, and SAP+DEX). The SAP mice model was established by ligature of pancreatic duct and intraperitoneal injection of cerulein. Renal perfusion and function, and morphological changes of the glycocalyx were evaluated by laser Doppler velocimetry, electron microscopy, and histopathology(hematoxylin and eosin(H&E) staining), respectively. Serum levels of syndecan-1 and TNF-α were assessed by enzyme-linked immunosorbent assay(ELISA). The proàtectiveì effects of dexamethasone on the glycocalyx and renal microcirculation were evaluated. Results: Significantly high levels of serum TNF-α were detected 3 h after the onset of SAP. These levels might induce degradation of the glycocalyx and kidney hypoperfusion, resulting in kidney microcirculation dysfunction. The application of dexamethasone reduced the degradation of the glycocalyx and improved perfusion of kidney. Conclusions: Dexamethasone protects the endothelial glycocalyx from inflammatory degradation possibly initiated by TNF-α during SAP. This is might be a significant discovery that helps to prevent tissue edema and hypoperfusion in the future. 展开更多
关键词 Severe acute pancreatitis(SAP) Acute kidney injury(AKI) glycocalyx DEXAMETHASONE Tumor necrosis factor-α(TNF-α)
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Effects of Cryopreservation on Human Sperm Glycocalyx
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作者 Yan-Cheng Wu Ai-Jie Xin +8 位作者 Hui Lu Hua Diao Li Cheng Yi-Hua Gu Bin Wu Sheng-Ce Tao Zheng Li Hui-Juan Shi Yong-Lian Zhang 《Reproductive and Developmental Medicine》 CSCD 2017年第4期233-238,共6页
Background:To study the effects of cryopreservation on human sperm glycocalyx.Methods:The lectin binding profilings of sperm after freeze-thaw were compared by lectin microarray.Results:CryoSperm^(TM) and direct fumig... Background:To study the effects of cryopreservation on human sperm glycocalyx.Methods:The lectin binding profilings of sperm after freeze-thaw were compared by lectin microarray.Results:CryoSperm^(TM) and direct fumigation were confirmed to be the optimized cryoprotectant and method by comparing the sperm recovery rate.In 91 lectins,33 lectins were significantly changed after sperm cryopreservation.Among them,9 lectins greatly decreased and 24 lectins mainly increased.The binding signals of MAA,PSA,ABA,and AIA were verified by FACS,and the results were consistent with that of lectin microarray.Conclusions:Sperm glycocalyx had significant changes after cryopreservation.The sialic acid,playing an important role in protecting sperm,was greatly lost,which exposed the inner carbohydrates.Thus,the glycocalyx damage due to the cryopreservation might be one of the reasons for low sperm fertility. 展开更多
关键词 Cryopreservation Damage glycocalyx Lectin Microarray SPERM
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衰老引发的糖萼损伤与血管功能障碍
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作者 张梓航 高朵岚 +4 位作者 戴鑫源 刘添 李明翰 邓小燕 康红艳 《医用生物力学》 CAS CSCD 北大核心 2024年第2期368-374,共7页
血管内皮糖萼(endothelial glycocalyx,EG)是位于血管内表面的一层多糖蛋白复合结构,其损伤与动脉粥样硬化、中风、脓毒症、糖尿病、肾脏疾病、高血压、肺水肿等诸多疾病存在关联。基于此,糖萼的健康程度已被列为评估血管健康的重要指... 血管内皮糖萼(endothelial glycocalyx,EG)是位于血管内表面的一层多糖蛋白复合结构,其损伤与动脉粥样硬化、中风、脓毒症、糖尿病、肾脏疾病、高血压、肺水肿等诸多疾病存在关联。基于此,糖萼的健康程度已被列为评估血管健康的重要指标。研究表明,衰老会伴随糖萼发生退行性改变,体现在其厚度变薄、合成及降解相关酶基因水平的失调等方面。作为血管的天然保护性屏障,衰老引发的糖萼损伤与血管舒缩功能受损、通透性增大、炎症及免疫反应失调、凝血/抗凝功能失衡等存在关联。从“结构决定功能”角度出发,开展衰老过程中糖萼厚度、成分、微观结构、力学特性等变化规律及其与血管功能障碍的相关性研究,对于预防、诊断、治疗动脉粥样硬化等年龄相关性心血管疾病具有重要意义。 展开更多
关键词 糖萼 衰老 血管功能障碍 动脉粥样硬化
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过劳诱导小鼠血管内皮屏障功能障碍
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作者 廖阅 马雪 +2 位作者 邓三春 陈苏衡 李玉兰 《南方医科大学学报》 CAS CSCD 北大核心 2024年第9期1814-1820,共7页
目的研究过劳对小鼠血管内皮屏障功能的影响。方法将30只KM小鼠随机分为3组(n=10):对照(CON)组、过劳2周(W2)组、过劳4周(W4)组。W2组和W4组采用水中站立+束缚实验分别连续造模2周和4周,观察小鼠一般情况。造模结束后,每组选取4只小鼠... 目的研究过劳对小鼠血管内皮屏障功能的影响。方法将30只KM小鼠随机分为3组(n=10):对照(CON)组、过劳2周(W2)组、过劳4周(W4)组。W2组和W4组采用水中站立+束缚实验分别连续造模2周和4周,观察小鼠一般情况。造模结束后,每组选取4只小鼠腹腔注射Evans蓝染料,检测血管通透性。另6只小鼠采取ELISA法检测血清IL-1β含量,获取动脉组织行切片观察,RT-qPCR法检测内皮细胞间紧密连接中occludin、claudin-5、ZO-1、JAM-A和黏附连接中VE-cadherin的mRNA表达水平;免疫荧光法检测内皮细胞间连接复合物中claudin-5、ZO-1、VE-cadherin和血管内皮糖萼中Syndecan-1的蛋白表达情况。结果与CON组相比,W2和W4组小鼠体质量增长缓慢,毛发脱落,活动减少。与CON组相比,W4组血管通透性明显增加。与CON组相比,W2和W4组血清IL-1β含量增加(P<0.05)。与CON组相比,W2组内皮细胞肿胀、脱落,内膜粗糙不规则,W4组出现内膜断裂现象。与CON组相比,W2组动脉组织中occludin、claudin-5、ZO-1、JAM-A的mRNA表达量增加,W4表达均降低(P<0.05);W2和W4组VE-cadherin的mRNA表达量下降(P<0.05)。与CON组相比,W4组claudin-5、ZO-1、VEcadherin和Syndecan-1蛋白表达减少(P<0.05)。结论过劳随时间的延长可对动脉内皮细胞间连接复合物以及内皮糖萼造成损伤,破坏其屏障功能,导致血管通透性增加。 展开更多
关键词 过劳 内皮屏障功能 内皮细胞间连接复合物 血管内皮糖萼
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糖萼损伤对炎症性肠病的诊断和监测价值
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作者 张乐丹 靳明星 刘艳迪 《天津医药》 CAS 2024年第8期893-896,共4页
炎症性肠病(IBD)目前无法治愈,只能通过药物、手术等手段缓解症状,延缓和减少复发,因此对疾病活动度的监测至关重要。糖萼是覆盖于血管内皮细胞管腔侧的多糖蛋白复合物,对于维持肠道黏膜屏障具有支持作用,而炎症会导致其结构受损。该文... 炎症性肠病(IBD)目前无法治愈,只能通过药物、手术等手段缓解症状,延缓和减少复发,因此对疾病活动度的监测至关重要。糖萼是覆盖于血管内皮细胞管腔侧的多糖蛋白复合物,对于维持肠道黏膜屏障具有支持作用,而炎症会导致其结构受损。该文介绍了目前临床上IBD在实验室检测中的无创性生物标志物,并分析了血清糖萼损伤标志物多配体蛋白聚糖1、硫酸乙酰肝素、透明质酸对IBD的诊断和监测价值。 展开更多
关键词 炎性肠疾病 肠黏膜 多配体蛋白聚糖1 硫酸乙酰肝素 透明质酸 糖萼损伤
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糖萼参与肾脏纤维化的机制
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作者 邢铭辉 许卉妍(综述) 李文斌(审校) 《肾脏病与透析肾移植杂志》 CAS CSCD 2024年第3期254-258,共5页
肾脏纤维化是慢性肾脏病(CKD)进展至终末期肾病的共同病理特征,明确肾脏纤维化发生的分子机制对CKD的防治至关重要。内皮细胞表层的多糖蛋白复合物——糖萼组成了血液和血管壁之间最基本的屏障。最新研究显示,糖萼在肾脏纤维化过程中发... 肾脏纤维化是慢性肾脏病(CKD)进展至终末期肾病的共同病理特征,明确肾脏纤维化发生的分子机制对CKD的防治至关重要。内皮细胞表层的多糖蛋白复合物——糖萼组成了血液和血管壁之间最基本的屏障。最新研究显示,糖萼在肾脏纤维化过程中发挥重要作用。本文就糖萼参与肾脏纤维化发生的相关研究进展作一综述,为其防治提供新的治疗策略。 展开更多
关键词 慢性肾脏病 肾脏纤维化 糖萼
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SphK/S1P在心血管疾病中的作用
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作者 唐丽鸿 陈春玲 《基础医学与临床》 CAS 2024年第8期1175-1179,共5页
磷酸鞘氨醇(S1P)是一种活性高的膜磷脂类代谢物,对心血管系统具有重要的生理作用。鞘氨醇激酶(SphK)是一种脂肪酶,对维持体内S1P水平的稳定非常重要。S1P通过催化调节与细胞表面的G蛋白偶联受体(S1PR1-5)的结合,从而在心血管疾病中发挥... 磷酸鞘氨醇(S1P)是一种活性高的膜磷脂类代谢物,对心血管系统具有重要的生理作用。鞘氨醇激酶(SphK)是一种脂肪酶,对维持体内S1P水平的稳定非常重要。S1P通过催化调节与细胞表面的G蛋白偶联受体(S1PR1-5)的结合,从而在心血管疾病中发挥不同的生理学效应,如参与调控动脉粥样硬化、心肌缺血再灌注损伤、心肌梗死、心力衰竭等疾病的发生发展,维持血管内皮细胞屏障功能,保护内皮糖萼层,白细胞黏附以及炎性反应等生理病理过程。 展开更多
关键词 1-磷酸鞘氨醇 鞘胺醇激酶 心血管疾病 内皮糖萼层
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体外循环围术期血管内皮多糖-蛋白复合物层损伤动态变化研究
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作者 罗远志 赵雷 +4 位作者 耿弘 籍强 左坤 周锴 何庚戌 《中国体外循环杂志》 2024年第5期387-394,共8页
目的 研究体外循环围术期血管内皮多糖-蛋白质复合物层(EGL)损伤标志物动态变化及其临床意义。方法 对连续44例行体外循环下心血管手术患者在术前(麻醉诱导前)、体外循环开始、体外循环结束、术后6 h、术后1 d、术后3 d、术后5 d、术后... 目的 研究体外循环围术期血管内皮多糖-蛋白质复合物层(EGL)损伤标志物动态变化及其临床意义。方法 对连续44例行体外循环下心血管手术患者在术前(麻醉诱导前)、体外循环开始、体外循环结束、术后6 h、术后1 d、术后3 d、术后5 d、术后7 d分别测量血浆血管EGL损伤标志物多配体蛋白复合物-1(Syndecan-1)、透明质酸(HA)、硫酸肝素(HS)、硫酸软骨素(CS)的浓度,在手术前后不同时间点测量动脉血气并记录乳酸浓度。采用方差分析比较各个时间点Syndecan-1、HA、HS、CS的浓度以及乳酸浓度是否具有统计学差异,并采用Pearson相关性分析比较EGL损伤标志物峰值浓度、乳酸峰值浓度、主动脉阻断时间、体外循环时间之间的相关性。结果 血管EGL损伤标志物Syndecan-1、HA、HS、CS的浓度在体外循环开始后即明显升高,在术后6 h均达到峰值,后逐渐下降并在术后7 d均降到与术前相近水平。患者动脉血乳酸浓度在术后6 h达到峰值,并在术后48 h恢复到术前水平。Syndecan-1、HA、HS、CS峰值浓度与主动脉阻断时间、体外循环时间、动脉血乳酸峰值水平呈正相关。结论 体外循环引起的血管EGL损伤可持续到术后一周的时间,且损伤的严重程度与体外循环时间、主动脉阻断时间、血浆乳酸峰值水平相关。 展开更多
关键词 体外循环 内皮多糖-蛋白质复合物层 乳酸 微循环 体外循环时间 主动脉阻断时间
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肠上皮糖萼与肠道疾病
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作者 高建伟 刘艳迪 靳明星 《世界华人消化杂志》 2024年第12期887-896,共10页
随着对糖生物学的不断研究,发现越来越多的疾病与糖萼相关.糖萼根据所在部位不同,大致可分为内皮糖萼与上皮糖萼.在过去的研究中多为内皮糖萼,本综述主要介绍肠上皮糖萼的生物学特性、其降解机制、与肠道不同疾病的生物相关性以及模拟... 随着对糖生物学的不断研究,发现越来越多的疾病与糖萼相关.糖萼根据所在部位不同,大致可分为内皮糖萼与上皮糖萼.在过去的研究中多为内皮糖萼,本综述主要介绍肠上皮糖萼的生物学特性、其降解机制、与肠道不同疾病的生物相关性以及模拟糖萼的纳米颗粒文库对器官的靶向递送药物等方面进行分析,以期为肠上皮糖萼在肠道疾病中潜在的诊断标志物以及治疗靶点的研究提供理论依据. 展开更多
关键词 肠上皮糖萼 结构 功能 黏膜屏障 肠道疾病 模拟糖萼的纳米颗粒
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术中液体治疗对内皮糖萼脱落影响的研究进展
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作者 王志瑶 苏国宁 尚舫 《临床麻醉学杂志》 CAS CSCD 北大核心 2024年第10期1095-1099,共5页
内皮糖萼(EG)是血管内皮屏障的重要组成部分,在体液稳态、炎症反应调节、维持血管通透性、血小板和白细胞粘附过程中起关键作用。创伤、炎症、感染和医源性干预是EG脱落的重要原因。液体治疗是麻醉科医师围术期管理的重要组成部分,根据... 内皮糖萼(EG)是血管内皮屏障的重要组成部分,在体液稳态、炎症反应调节、维持血管通透性、血小板和白细胞粘附过程中起关键作用。创伤、炎症、感染和医源性干预是EG脱落的重要原因。液体治疗是麻醉科医师围术期管理的重要组成部分,根据不同的患者选择适当的液体类型和液体输注策略可能有助于降低术后并发症的发生率,改善患者预后。本文对EG的结构和生理特性进行阐述,对术中液体治疗的现状进行归纳,并探讨术中液体治疗对EG的影响以及液体治疗的优化对EG的保护作用,为术中液体治疗提供参考。 展开更多
关键词 液体治疗 内皮糖萼 晶体 胶体
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糖萼与动脉粥样硬化的研究进展
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作者 努尔柯孜·阿卜杜合力力 吴弘 《中国动脉硬化杂志》 CAS 2024年第11期994-998,共5页
动脉粥样硬化(As)是心脑血管疾病中最常见的病变,血管内皮功能障碍是As的初始阶段,糖萼(GCX)作为血管内皮表面覆盖的物理屏障,可通过调节血管通透性、舒张功能、细胞间通讯及炎症细胞黏附等,参与As的发生发展。因此,保持血管内皮GCX完... 动脉粥样硬化(As)是心脑血管疾病中最常见的病变,血管内皮功能障碍是As的初始阶段,糖萼(GCX)作为血管内皮表面覆盖的物理屏障,可通过调节血管通透性、舒张功能、细胞间通讯及炎症细胞黏附等,参与As的发生发展。因此,保持血管内皮GCX完整性可能是As治疗的潜在靶点。此外,GCX脱落水平可反映As严重程度。因此,GCX降解产物水平(如硫酸乙酰肝素、透明质酸等)可能用于评估As的严重程度。本文就GCX在As发生中的研究进展进行综述。 展开更多
关键词 动脉粥样硬化 糖萼 硫酸乙酰肝素 透明质酸
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靶向血管内皮糖萼的抗休克药物
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作者 曾滨 鲁晓红 姚立农 《临床与病理杂志》 CAS 2024年第1期101-107,共7页
内皮糖萼(endothelial glycocalyx,EG)是位于血管内皮腔内侧的1层带负电荷的绒毛状结构,为一种由多糖分子与蛋白质结合而成的复合物。血管EG作为休克期间保护血管内皮的重要屏障,可发挥调节血管张力、调控细胞信号转导、维持血管完整性... 内皮糖萼(endothelial glycocalyx,EG)是位于血管内皮腔内侧的1层带负电荷的绒毛状结构,为一种由多糖分子与蛋白质结合而成的复合物。血管EG作为休克期间保护血管内皮的重要屏障,可发挥调节血管张力、调控细胞信号转导、维持血管完整性、调节炎症及凝血障碍等重要生理功能,从而维持机体血管内稳态。休克发生时EG的结构破坏、脱落是微循环障碍的主要原因之一。靶向EG的抗休克药物主要通过抑制EG降解酶、炎症因子的表达等途径减少EG组分的脱落,维持内皮结构完整性,改善微血管通透性来治疗休克。深入探究血管EG的具体损伤机制及其治疗靶点,可为靶向EG的药物探索和休克的临床治疗提供理论依据和治疗参考。 展开更多
关键词 休克 血管内皮 内皮糖萼
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亚胺培南联合阿奇霉素治疗铜绿假单胞菌生物被膜感染的实验研究 被引量:34
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作者 方向群 刘又宁 《中华医院感染学杂志》 CAS CSCD 北大核心 2007年第3期254-257,共4页
目的研究亚胺培南(IMP)联合阿奇霉素(AZM)在治疗铜绿假单胞菌生物被膜(BF)感染中的作用。方法色氨酸法测定AZM对多糖蛋白复合物(GLX)的抑制作用;通过噻唑蓝(MTT)法测定BF上的活菌数,比较IMP与AZM联合应用时对BF细菌的体外抑制作用;采用... 目的研究亚胺培南(IMP)联合阿奇霉素(AZM)在治疗铜绿假单胞菌生物被膜(BF)感染中的作用。方法色氨酸法测定AZM对多糖蛋白复合物(GLX)的抑制作用;通过噻唑蓝(MTT)法测定BF上的活菌数,比较IMP与AZM联合应用时对BF细菌的体外抑制作用;采用组织笼大鼠皮下埋植法建立细菌BF体内局部感染动物模型,并考察AZM对IMP的体内协同作用。结果AZM可以使细菌BF的GLX的含量显著地下降;在IMP浓度相同的情况下,加入1/4、1/16 MIC的AZM可使BF上的活菌数显著地减少;IMP和AZM联合应用时,铜绿假单胞菌BF感染局部的活菌数较IMP单独作用时显著减少。结论AZM可以在治疗铜绿假单胞菌BF感染中对IMP起到协同作用。 展开更多
关键词 生物被膜 多糖蛋白复合物 大环内酯类 亚胺培南
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海带硫酸多糖蛋白复合物的气相色谱分析 被引量:6
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作者 王长振 丛建波 +2 位作者 先宏 孙存普 吴可 《食品科学》 EI CAS CSCD 北大核心 2007年第2期261-263,共3页
为开发海洋资源,对海带有效成分海带硫酸多糖蛋白复合物(glycocalyx of sulfated polysaccharide from Lami-naria japonica,GPL)的结构组成进行分析。GPL为本实验室制备,经检测GPL中硫酸基含量为29.12%,总糖27.8%,蛋白质12.69%。通过... 为开发海洋资源,对海带有效成分海带硫酸多糖蛋白复合物(glycocalyx of sulfated polysaccharide from Lami-naria japonica,GPL)的结构组成进行分析。GPL为本实验室制备,经检测GPL中硫酸基含量为29.12%,总糖27.8%,蛋白质12.69%。通过衍生化处理,然后采用气相色谱仪分析该多糖蛋白复合物的单糖组成。结果表明GPL是主要有岩藻糖聚合而成的复合物,另外含有甘露糖、半乳糖、葡萄糖和木糖等。 展开更多
关键词 海带硫酸多糖蛋白复合物 乙酰化 气相色谱
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